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Last updated: 2022/11/18

Bombyx mori Library Information

Gene model    Old gene model (2008)    The complete sequences of the FL-cDNA clones    cDNA   
Assembled RNA-seq    ORF from Assembled RNA-seq   piRNA    ChIP-seq    SAGE    MPSS   Transposon   
Genome    Old scaffold    Fosmid   BAC
Hypothetically reconstructed genomes of 18 Bombyx mori strains    18 Re-sequenced Genome DNA libraries (Partial RAW Reads)

Gene model libraries

Lib. name #clones Description
KWMTBOMO 16880GeneModel based on the new assembled genome
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Old gene model (2008) libraries

Lib. name #clones Description
BGIBMGA 14623GeneModel based on scaffold build2
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The complete sequences of the FL-cDNA clone libraries

Lib. name #clones Description
Complete_Seq11833The complete sequences of the FL-cDNA clones
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cDNA libraries

Lib. name #clones Strain/Race Organ/Tissue Development stage Sex Vector Cloning site Sequence direction
an-- 606  Strain: Shuko x Ryuhaku  Antenna  Adult moth    pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
bmmt 21180  p50T  Malpighan tube  5th instar larva, spinning stage: day-0 to day-6 mixed  Female/male mixed  pGCAP10,V-capping method    single-pass sequencing from both ends (5' end 3') 
BmN 20736  Strain: Unknown; BmN cultured cell  cultured cell (derived from ovary)      Oligo-cap, full-length    sequenced from 5' 
bmnc 20635  BmN4  cultured cell (derived from ovary)  NPV infected; 20hr postinfection    pGCAP10,V-capping method    single-pass sequencing from both ends (5' end 3') 
bmov 21573  p50T  ovary  P4  Female  pGCAP10,V-capping method    single-pass sequencing from both ends (5' end 3') 
bmte 21503  p50T  testis  P4  Male  pGCAP10,V-capping method    single-pass sequencing from both ends (5' end 3') 
br-- 1017  Strain: p50  Brain  P0 (just after pupation)  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
brP- 1587  Strain: p50  Brain  P1-P7  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
brS- 798  Strain: p50  Brain  Larva of S0-S4  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
caL- 10831  Strain: p50  corpora allata  5th instar larva  Female/male mixed  pBluescript SK-  EcoR1 for 5' Xho1for 3'  sequenced from T3 primer (5' -> 3') 
ce-- 1621  Strain: C202 x J201  Compound eyes  Mixed stages 5th instar to pupa  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
ceN- 3812  The same as ce-- but a different preparation  Compound eyes  Mixed stages 5th instar to pupa  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
cesb 83  The same as ce--: Subtracted library from ce-- library with the mixed probe containing 20 highly expressed housekeeping genes  Compound eyes  Mixed stages 5th instar to pupa  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
e40h 756  Strain: p50  Diapause-destined embryo  40 hr after oviposition  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
e96h 710  Strain: p50  Diapause-destined embryo  96 hr after HCl treatment (HCl at 20 hr after oviposition)  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
epV3 1969  Strain: p50  Epidemis  5th-instar day-3 larva  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
F1mg 2128  Strain: F1 (J150 x J203)  midgut  fourth instar larval stage D2  Female/male mixed  pBluescript SK-  EcoR1 for 5' Xho1for 3'  sequenced from T3 primer (5' -> 3') 
famL 25972  Strain: N137 X C146  mixture of antenna and maxillary galea  fifth instar larval stage (day-2 to day-3)  Female/male mixed  pGCAP10  V-capping  full-length 
fbpv 698  Strain: Shuko x Ryuhaku  fat body  S2 (Spinning day 2); BmNPV infected; 2 hr postinfection  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
fbS2 281  Strain: C202 x J201  fat body  S2 (Spinning day 2)  Male  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
fbVf 298  Strain: p50  fat body  5th-instar day-3 larva  Female  cloned into pUC18 SmaI  SmaI-Blunt-end  primer (direction unknown) 
fbVm 708  Strain: p50  fat body  5th-instar day-3 larva  Male  cloned into pUC18 SmaI  SmaI-Blunt-end  primer (direction unknown) 
fcaL 33462  Strain: p50T  corpora allata  5th instar larvae    pGCAP1,V-capping method    single-pass sequencing from both ends (5' end 3') 
fcP8 3779  Strain: p50T  follicle cells  pupal stage P8  Female  pBluescript SK-  EcoR1 for 5' Xho1for 3'  sequenced from T3 primer (5' -> 3') 
fdpe 9984    diapause-destinated embryos  12-40hr after oviposition  Female/male mixed  pGCAPI, G-capping, full-length    Sequenced from 5' with T8 primer 
fe100 5024  Strain: p50T  whole egg includeing embryo  100 hours after oviposition    pGCAP1,V-capping method    single-pass sequencing from both ends (5' end 3') 
fe8d 18731  Strain: p50T  embryo  fertilized egg stage H200  Female/male mixed  pGCAP10  V-capping  full-length 
fepM 5620  Strain: p50T  epidermis  fourth molting stage  Female/male mixed  pGCAPI  G-capping  full-length 
ffbm 9843  Strain: p50T  fat body  fifth instar larval stage  Male  pGCAP10  V-capping  full-length 
FJsb 1030  Strain: J150-F1  Midgut  4th-instar larval stage  Female/male mixed  pBluescript SK-  EcoR1 for 5' Xho1for 3'  sequenced from T3 primer (5' -> 3') 
fmgV 38020  Strain: p50T  midgut  5th instar larvae    pGCAP1,V-capping method    single-pass sequencing from both ends (5' end 3') 
fmxg 4370  Strain: p50T  maxillary galea  fifth instar larval stage D3  Female/male mixed  pGCAPI  G-capping  full-length 
fner 37074  Strain: p50T  ventral nerve cord including brain  5th instar larvae    pGCAP1,V-capping method    single-pass sequencing from both ends (5' end 3') 
fphe 334  Strain: p50T  pheromone gland  adult stage D0  Female  pGCAPI  G-capping  full-length 
fprw 6008  Strain: p50T  prothoracic gland  5th instar larvae    pGCAP1,V-capping method    single-pass sequencing from both ends (5' end 3') 
ftes 29670  Strain: p50T  testis  fifth instar larval stage  Male  pGCAP10  V-capping  full-length 
fufe 17095  Strain: p50T  embryo  unfertilized egg stage  Female/male mixed  pGCAP10  V-capping  full-length 
FWD 24960  Strain: p50T  Wing disc  5th-instar larva - spinning stage: mixed  Female/male mixed  Oligo-cap, full-length    sequenced from 5' 
fwgP 17521  Strain: p50T  wing  pupal stage  Female/male mixed  pGCAP10  V-capping  full-length 
heS0 831  Strain: C108  Hemocyte  Spinning stage day-0  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
heS3 606  Strain: C108  Hemocyte  Spinning stage day-3  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
J150 2847  Strain: J150  midgut  fifth instar larval stage D2  Female/male mixed  pBluescript SK-  EcoR1 for 5' Xho1for 3'  sequenced from T3 primer (5' -> 3') 
JFsb 500  Strain: J150-F1  midgut  fifth instar larval stage D3  Female/male mixed  pBluescript SK-  EcoR1 for 5' Xho1for 3'  sequenced from T3 primer (5' -> 3') 
maV3 1697  Strain: p50  Malpighian tubule  5th-instar larval stage D3  Female/male mixed  pBluescript SK-  EcoR1 for 5' Xho1for 3'  sequenced from T3 primer (5' -> 3') 
MFB 10368  Strain: p51T  Microbe-infected fat body  5th-instar larva  Female/male mixed  pGCAPI, G-capping, full-length    Sequenced from 5' with T9 primer 
mg-- 605  Strain: p50  Midgut  5th-instar day-3 larva  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
msgV 645  Strain: N02 x C02  Middle silkgland  5th-instar larva  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
N--- 757  Strain: Unknown; BmN clutured cell  cultured cell (derived from ovary)      pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
Nnor 221  BmN normalized library  Ovary-derived cell-line      pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
NRPG 1521  Strain: p50, Normalized Library  Pheromone gland  Adult  Female  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
NV02 558  Strain: Unknown, BmN cultured cell  cultured cell (derived from ovary)  BmNPV infected; 2 hr postinfection    pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
NV06 684  Strain: Unknown, BmN cultured cell  cultured cell (derived from ovary)  BmNPV infected; 6 hr postinfection    pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
NV12 646  Strain: Unknown, BmN cultured cell  cultured cell (derived from ovary)  BmNPV infected; 12 hr postinfection    pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
ovS0 2600  Strain: p50T  ovary  spinning stage S0  Female  pBluescript SK-  EcoR1 for 5' Xho1for 3'  sequenced from T3 primer (5' -> 3') 
ovS3 2709  Strain: p50  ovary  Spinning stage S3  Female  pBluescript SK-  EcoR1 for 5' Xho1for 3'  sequenced from T3 primer (5' -> 3') 
P5PG 811  Strain: p50  Pheromone gland  Pupation day-5 stage  Female  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
pg-- 553  Strain: Shuko x Ryuhaku  Pheromone gland  Newly-eclosed adult  Female  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
prgv 813  Strain: p50  Prothoracic gland  5th-instar day-4 larva  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
ps4M 312  Strain: p50  Posterior silkgland  4th molting stage  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
psV3 740  Strain: p50  Posterior silkgland  5th-instar day-3 larva    pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
tesS 944  Strain: p50  testis  Spinning stage day-3  Male  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
tesV 324  Strain: p50  testis  5th-instar day-3 larva  Male  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
vg4M 2481  Strain: p50T  Verson's gland  fourth molting stage D2  Male  pBluescript SK-  EcoR1 for 5' Xho1for 3'  sequenced from T3 primer (5' -> 3') 
wdS0 866  Strain: C108  Wing disc  Spinning stage day-0  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
wdS2 760  Strain: C108  Wing disc  Spinning stage day-2  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
wdS3 774  Strain: C108  Wing disc  Spinning stage day-3  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
wdV1 327  Strain: C108  Wing disc  5th-instar day-1 larva  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
wdV3 843  Strain: C108  Wing disc  5th-instar day-3 larva  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
wdV4 749  Strain: C108  Wing disc  5th-instar day-4 larva  Female/male mixed  pBluescript SK-  EcoR1 for 5', Xho1for 3'  sequenced from T3 primer (5' -> 3') 
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Assembled RNA-seq libraries

Lib. name #reads Strain Organ/Tissue Development stage Sex
A_BomoASG 58621  p50T  Anterior silk gland  5th instar  Female/male mixed 
A_BomoBR 206205  p50T  Brain  Adult (virgin 0h + 10h)  Female 
A_BomoEE 221677  p50T  Whole  Early embryo  Female/male mixed 
A_BomoFB 35635  p50T  Fat body  5th instar  Female/male mixed 
A_BomoIG 206471  p50T  Internal genitalia  Adult (virgin 0h + 10h)  Female 
A_BomoMG 43914  p50T  Midgut  5th instar  Female/male mixed 
A_BomoMSG 47701  p50T  Middle silk gland  5th instar  Female/male mixed 
A_BomoN4EE 144953  N4  Whole  Early embryo  Female/male mixed 
A_BomoOtmSK 47999  otm  Epidermis  5th instar  Female/male mixed 
A_BomoSK 49310  p50T  Epidermis  5th instar  Female/male mixed 
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ORF from assembled RNA-seq libraries

Lib. name #reads Strain Organ/Tissue Development stage Sex
O_BomoASG 32479  p50T  Anterior silk gland  5th instar  Female/male mixed 
O_BomoBR 103968  p50T  Brain  Adult (virgin 0h + 10h)  Female 
O_BomoEE 95730  p50T  Whole  Early embryo  Female/male mixed 
O_BomoFB 25536  p50T  Fat body  5th instar  Female/male mixed 
O_BomoIG 104180  p50T  Internal genitalia  Adult (virgin 0h + 10h)  Female 
O_BomoMG 16985  p50T  Midgut  5th instar  Female/male mixed 
O_BomoMSG 29137  p50T  Middle silk gland  5th instar  Female/male mixed 
O_BomoN4EE 51699  N4  Whole  Early embryo  Female/male mixed 
O_BomoOtmSK 35878  otm  Epidermis  5th instar  Female/male mixed 
O_BomoSK 33939  p50T  Epidermis  5th instar  Female/male mixed 
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piRNA libraries

Lib. name #reads Strain Organ/Tissue Development stage Sex
OV687407p50TOvary-Female
TE601621p50TTestis-Male
MW7869133MW (Bombyx mori with Bombyx mandarina W chromosome)Ovary-Female
WF7766937WF (DfZ-DfW) (Fujii et al. 2006)Testis-Male
LY8485411LY (T(W:2)Y-Abe) (Abe et al. 2008)Ovary-Female
Siwi3566756p50TWhole--
BmAgo32497217p50TWhole--
GFP #89548321----
0h Egg8987706p50TWhole0 hour post-fertilization embryo-
6h Egg9724369p50TWhole6 hours post-fertilization embryo-
12h Egg13619042p50TWhole12 hours post-fertilization embryo-
24h Egg10304686p50TWhole24 hours post-fertilization embryo-
40h Diapause Egg325606----
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ChIP-seq libraries

Lib. name #reads Strain Description
Input22560725BmN4-
pIZ33593500BmN4Control for BmHP1a and Cdp1
BmHP1a38801571BmN4Bombyx mori heterochromatin protein 1 homolog
Cdp136251377BmN4Chromodomain protein 1
IgG-R36337532BmN4Rabbit IgG (Control for H3K4me1, H3K4me2, H3K4me3, H3K9ac, H3K9me2, H3K9me3, H3K27ac, H3K27me3, and H3K36me3)
H3K4me131735270BmN4Histone H3 monomethyl Lys4 (Euchromatic modifications)
H3K4me237613798BmN4Histone H3 dimethyl Lys4 (Euchromatic modifications)
H3K4me325595873BmN4Histone H3 trimethyl Lys4 (Euchromatic modifications)
H3K9ac35094666BmN4Histone H3 acetyl Lys9 (Euchromatic modifications)
H3K9me229450531BmN4Histone H3 dimethyl Lys9 (Heterochromatic modifications)
H3K9me334783792BmN4Histone H3 trimethyl Lys9 (Heterochromatic modifications)
H3K27ac34707965BmN4Histone H3 acetyl Lys27 (Euchromatic modifications)
H3K27me334719405BmN4Histone H3 trimethyl Lys27 (Heterochromatic modifications)
H3K36me333361047BmN4Histone H3 trimethyl Lys36 (Heterochromatic modifications)
IgG-M27303327BmN4Mouse IgG (Control for Pol2)
Pol234323768BmN4RNA polymerase II
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SAGE libraries

Lib. name #clones Strain Organ/Tissue Development stage Sex
3'SAGE57051p50TOviduct (germarium to stage3)PupaFemale
5'SAGE25176p50Twhole96 hours after oviposition-
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MPSS libraries

Lib. name #clones Strain Organ/Tissue Development stage Sex
MPSS44872p50TOviduct (germarium to stage3)PupaFemale
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Transposon libraries

Lib. name #clones Description
121TEs121121 well-annotated transposons
BmTE16901690 transposons library
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Genome library

Lib. name #clones Description
Bomo_Chr28Genome assembly (2017) sequencing was performed on the PacBio RS II. Chromosome number was determined. Scaffolds were connected with N.
Bomo_Scaf668Genome assembly (2017) sequencing was performed on the PacBio RS II. Chromosome number was not determined.
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Old scaffold library

Lib. name #clones Description
Bm_scaf43462Scaffold build2 (2008)
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Fosmid library

Lib. name #clones Description
RO, GBMFno274342Fosmid end
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BAC libraries

Lib. name #clones Description
BE137219BAC end
BAC55BAC (assembled)
W-BAC_BABU479W chromosome-derived BAC
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Hypothetically reconstructed genomes of 18 Bombyx mori strains

Lib. name #clones Strain Geographic race Sex
R_C108T696C108T (p62)Chinese practical racesMale
R_N4696N4 (p55)Tropical polyvoltinismMale
R_b20696b20Ryukyu localMale
R_c10696c10Japanese bivoltineMale
R_c51696c51South Chinese localMale
R_d18696d18Korean univoltineMale
R_e10696e10-Male
R_f35696f35Chinese bivoltineMale
R_g53696g53-Male
R_k25696k25Chinese univoltineMale
R_n16696n16Japanese and Europian practical racesMale
R_o55696o55Japanese bivoltineMale
R_o56696o56Chinese univoltineMale
R_p20696p20Japanese univoltineMale
R_p21696p21Japanese bivoltineMale
R_p22696p22Japanese bivoltineMale
R_p44696p44Chinese practical racesMale
R_u48696u48European univoltineMale
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18 Re-sequenced Genome DNA libraries (Partial RAW Reads)

Lib. name #reads Strain Geographic race Sex
DNA_C108T101790778C108T (p62)Chinese practical racesMale
DNA_N493536086N4 (p55)Tropical polyvoltinismMale
DNA_b2087439810b20Ryukyu localMale
DNA_c1094615302c10Japanese bivoltineMale
DNA_c5187443686c51South Chinese localMale
DNA_d1886189734d18Korean univoltineMale
DNA_e1093725210e10-Male
DNA_f3586379202f35Chinese bivoltineMale
DNA_g5399718872g53-Male
DNA_k2591863488k25Chinese univoltineMale
DNA_n1692723530n16Japanese and Europian practical racesMale
DNA_o55106945032o55Japanese bivoltineMale
DNA_o5693238876o56Chinese univoltineMale
DNA_p2094862490p20Japanese univoltineMale
DNA_p2187157824p21Japanese bivoltineMale
DNA_p2284086750p22Japanese bivoltineMale
DNA_p4494415124p44Chinese practical racesMale
DNA_u4895997146u48European univoltineMale
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