Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA005448-TA|BGIBMGA005448-PA|IPR008994|Nucleic
acid-binding, OB-fold, IPR004591|Replication factor-a protein 1 Rpa1,
IPR007199|Replication factor-A protein 1, N-terminal,
IPR004365|nucleic acid binding, OB-fold, tRNA/helicase-type,
IPR013955|Replication factor-A, C-terminal
(585 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit S... 383 e-107
SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces... 33 0.11
SPCC1919.04 |||sequence orphan|Schizosaccharomyces pombe|chr 3||... 29 2.4
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 29 2.4
SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein k... 28 3.1
SPAC1805.17 |crm1|caf2, SPAC1B2.01|nuclear export receptor Crm1|... 28 4.1
SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces pom... 28 4.1
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 27 5.5
SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo... 27 7.2
SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase... 27 9.5
SPBC359.04c |||DIPSY family|Schizosaccharomyces pombe|chr 2|||Ma... 27 9.5
>SPBC660.13c |ssb1|rpa1, rad11|DNA replication factor A subunit Ssb1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 609
Score = 383 bits (943), Expect = e-107
Identities = 207/594 (34%), Positives = 339/594 (57%), Gaps = 32/594 (5%)
Query: 9 PIIQMLGTKKIQGND---AGDRYRLLVSDGKYTYSFAMLATQLNIMLLSGELTDYSVIQV 65
PI+Q+L K++ N A RYR+++SD Y+ +ML+TQLN ++ +L + +Q+
Sbjct: 25 PILQVLTVKELNSNPTSGAPKRYRVVLSDS-INYAQSMLSTQLNHLVAENKLQKGAFVQL 83
Query: 66 DQFVTTLLKNVGKGEKRVIIILALTIIANGRDVRHKIGNP---------QPWSEEATSAQ 116
QF ++K E++++I+L L ++ V KIGNP + E +A
Sbjct: 84 TQFTVNVMK-----ERKILIVLGLNVLTE-LGVMDKIGNPAGLETVDALRQQQNEQNNAS 137
Query: 117 RPEPKPAPVTPKF-------TAKVEPKAANLDSSMLSVK-MTNPISSLSPYQNKWVIRAR 168
P + T F TA P ++ S+ + PI LSPYQNKW IRAR
Sbjct: 138 APRTGISTSTNSFYGNNAAATAPAPPPMMKKPAAPNSLSTIIYPIEGLSPYQNKWTIRAR 197
Query: 169 VMSKTPIRTWSNAKGEGKLFSMYLCDETGEIRATAFKNECDTFYDMIQVDKVYYISRCAI 228
V +K+ ++ W N +GEGKLFS+ L DE+GEIRAT F ++ D FYD++Q VYYISRC +
Sbjct: 198 VTNKSEVKHWHNQRGEGKLFSVNLLDESGEIRATGFNDQVDAFYDILQEGSVYYISRCRV 257
Query: 229 KTANKKFTTLKNDYEMTFTAETVVGEYVGDESAVPTVQYDFLPISEIANKETETLIDLIG 288
A K++T ++N+YE+ F +T + + D++AVP ++ F+ + E+ + + +ID+IG
Sbjct: 258 NIAKKQYTNVQNEYELMFERDTEIRK-AEDQTAVPVAKFSFVSLQEVGDVAKDAVIDVIG 316
Query: 289 VCKSASDVQELTAKSTGKLLKKREVMLVDASGGAVTLTLWGNEAEKFEGNTNPILAVKGA 348
V ++ VQ++T+++T + KR++ +VD +G + +TLWG A +F + ILA KG
Sbjct: 317 VLQNVGPVQQITSRATSRGFDKRDITIVDQTGYEMRVTLWGKTAIEFSVSEESILAFKGV 376
Query: 349 RLTEFNGSKSLSCLSSTMLRLNPDVPEAHKLRGWYDNGGADADIVNISAKSGNF--TGGS 406
++ +F G +SLS L+S+ + ++PD+ E+H L+GWYD G + S S TG S
Sbjct: 377 KVNDFQG-RSLSMLTSSTMSVDPDIQESHLLKGWYDGQGRGQEFAKHSVISSTLSTTGRS 435
Query: 407 NEWISFSEAESRQLGTGEKGDYYSLLGVLTFTFSENAVYKACPQEQCNKKLVDQENGLFR 466
E + +E ++ LG E DY+SL G + + +N Y ACP CNKK+ DQ G +R
Sbjct: 436 AERKNIAEVQAEHLGMSETPDYFSLKGTIVYIRKKNVSYPACPAADCNKKVFDQ-GGSWR 494
Query: 467 CEKCNREYPNYKHRILLAANVSDPTGDQRVTLFNEAAETMLGKSAEEIARLSEYDKNEYN 526
CEKCN+EY ++R ++ V D TG + +F++ + ++ K+A+E+ L E D+N +
Sbjct: 495 CEKCNKEYDAPQYRYIITIAVGDHTGQLWLNVFDDVGKLIMHKTADELNDLQENDENAFM 554
Query: 527 KIFDEXXXXXXXXXXXXXMENFNDESRLKTTVVNVQPVDYKDGIARLLKNIKTA 580
E +NF E R++ TV+++ +D+K+ RL+ I++A
Sbjct: 555 NCMAEACYMPYIFQCRAKQDNFKGEMRVRYTVMSINQMDWKEESKRLINFIESA 608
>SPAC1486.04c |alm1||medial ring protein Alm1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1727
Score = 33.1 bits (72), Expect = 0.11
Identities = 20/70 (28%), Positives = 33/70 (47%), Gaps = 2/70 (2%)
Query: 450 QEQCNKKLVDQENGLFRCEKCNREYPNYKHRILLAANVSDPTGDQRVTLFNE--AAETML 507
+EQ +L ++ + + E N EY + ILL+ N S T L NE + E ++
Sbjct: 1274 KEQLESQLQTEKAAVKKLENSNEEYKRHNQEILLSLNSSTSTSSDASRLKNELVSKENLI 1333
Query: 508 GKSAEEIARL 517
+ +EI L
Sbjct: 1334 EELNQEIGHL 1343
>SPCC1919.04 |||sequence orphan|Schizosaccharomyces pombe|chr
3|||Manual
Length = 256
Score = 28.7 bits (61), Expect = 2.4
Identities = 29/95 (30%), Positives = 40/95 (42%), Gaps = 10/95 (10%)
Query: 282 TLIDLIGVCKSASDVQE-LTAKSTGKLL----KKREVMLVDASGGAVTLTLWGNEAEKFE 336
T ID S S V E +T K+L K R +ML D + + ++EK +
Sbjct: 135 TFIDSFATPSSNSTVTEAMTKMKNEKVLEYPYKDRNIMLGDLAS-----RIPSKDSEKLD 189
Query: 337 GNTNPILAVKGARLTEFNGSKSLSCLSSTMLRLNP 371
PI K L + S S S SST L++ P
Sbjct: 190 EERQPIALEKTKSLDSISHSSSSSRKSSTELKIPP 224
>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 468
Score = 28.7 bits (61), Expect = 2.4
Identities = 13/33 (39%), Positives = 17/33 (51%)
Query: 457 LVDQENGLFRCEKCNREYPNYKHRILLAANVSD 489
LVD E+G+ RC +C E N H +SD
Sbjct: 184 LVDSEDGVLRCARCQWELENPYHCDHCGFQISD 216
>SPAC16C9.07 |ppk5|SPAC2G11.01, mug189|serine/threonine protein
kinase Ppk5 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 836
Score = 28.3 bits (60), Expect = 3.1
Identities = 13/32 (40%), Positives = 20/32 (62%), Gaps = 2/32 (6%)
Query: 437 FTFSENA--VYKACPQEQCNKKLVDQENGLFR 466
FT SEN + K P ++CN+ L D +NG ++
Sbjct: 476 FTGSENCQKLSKQIPLDECNEALFDDDNGDYK 507
>SPAC1805.17 |crm1|caf2, SPAC1B2.01|nuclear export receptor
Crm1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1078
Score = 27.9 bits (59), Expect = 4.1
Identities = 18/43 (41%), Positives = 22/43 (51%)
Query: 50 IMLLSGELTDYSVIQVDQFVTTLLKNVGKGEKRVIIILALTII 92
+ LLS E+ DYS Q+ Q T LKN GE I L I+
Sbjct: 159 LRLLSEEIFDYSAEQMTQLKTKNLKNQMCGEFAEIFQLCSQIL 201
>SPAC23D3.06c |nup146||nucleoporin Nup146|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1325
Score = 27.9 bits (59), Expect = 4.1
Identities = 13/67 (19%), Positives = 34/67 (50%), Gaps = 1/67 (1%)
Query: 228 IKTANKKFTTLKNDYEMTFTAETVVGEYVGDESAVPTVQYDFLPISEIANKETETLIDLI 287
+ T + + KN+ ++ + ++ EY+ +++ P+ + ++ + ++ TE L DL+
Sbjct: 1074 VNTFERLYLQFKNELDLVWQNINIIAEYIEGQTSTPSAVSEQATMA-MLSQNTEELEDLL 1132
Query: 288 GVCKSAS 294
S S
Sbjct: 1133 DTVTSFS 1139
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 27.5 bits (58), Expect = 5.5
Identities = 22/65 (33%), Positives = 29/65 (44%), Gaps = 11/65 (16%)
Query: 103 GNPQPWSEEATSAQRPEPKPAP-------VTPKFTAKVEPKAANLDSSMLSVKMTNPISS 155
G+P PW+ +TS PAP P A + AA + LS+ NP SS
Sbjct: 404 GHPPPWAAASTSVSSSTSSPAPWAKPASSAAPSNPAPWQQPAAPQSAPALSM---NP-SS 459
Query: 156 LSPYQ 160
L P+Q
Sbjct: 460 LPPWQ 464
>SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase
Swr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1288
Score = 27.1 bits (57), Expect = 7.2
Identities = 19/73 (26%), Positives = 32/73 (43%), Gaps = 3/73 (4%)
Query: 162 KWVIRARVMSKTPIRTWSNAKGEGKLFSMYLCDETGEIRATAFKNECDTFYDMIQVDKVY 221
K ++ M+K + T + + + F ++ DE + AT N TF M Q+D+
Sbjct: 774 KRLLHEEPMTKLDLSTLRLIRTDSEAFDTFVSDELNSLCATNAYNRISTFLRM-QIDE-- 830
Query: 222 YISRCAIKTANKK 234
C K +N K
Sbjct: 831 ECKPCQFKKSNFK 843
>SPAC10F6.17c ||SPAC56E4.01c|mitochondrial pyruvate dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 444
Score = 26.6 bits (56), Expect = 9.5
Identities = 17/52 (32%), Positives = 28/52 (53%), Gaps = 4/52 (7%)
Query: 478 KHRILLAANVS---DPTGDQRVTLFNEAAETMLGKSAEEIARLSEYDKNEYN 526
KHR L+ A+ + +Q V L E A+T+LGK+ E ++ DK ++
Sbjct: 335 KHRFLIMASDGLWDTMSSEQAVQLVGEWADTVLGKTTNE-KNTTQDDKQSWS 385
>SPBC359.04c |||DIPSY family|Schizosaccharomyces pombe|chr
2|||Manual
Length = 358
Score = 26.6 bits (56), Expect = 9.5
Identities = 28/124 (22%), Positives = 49/124 (39%), Gaps = 6/124 (4%)
Query: 140 LDSSMLSVKMTNPISSLSPYQNKWVIRARVMSKTPIRTWSNAKGEGKLFSMYLCDETGEI 199
L +S +S + PI++ P + + + + + +P+ T S+ + S + I
Sbjct: 86 LSNSTISTSSSTPITASVPTSSSILSNSTIPTTSPVPTTSSTPTSSSILSNSTIPSSSSI 145
Query: 200 RATAFKNEC---DTFYDMIQVDKVYYISRCAIKTANKKFTTLK--NDYEM-TFTAETVVG 253
A+ T + VD+ I TA TTL + Y + T T +TV G
Sbjct: 146 SASTITTTIISGSTQFTTTFVDQSIDTVEVVIPTAGYITTTLTSGSSYPVSTTTLQTVSG 205
Query: 254 EYVG 257
G
Sbjct: 206 TQSG 209
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.314 0.131 0.375
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,582,147
Number of Sequences: 5004
Number of extensions: 107641
Number of successful extensions: 212
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 201
Number of HSP's gapped (non-prelim): 14
length of query: 585
length of database: 2,362,478
effective HSP length: 77
effective length of query: 508
effective length of database: 1,977,170
effective search space: 1004402360
effective search space used: 1004402360
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (22.0 bits)
S2: 56 (26.6 bits)
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