Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA004687-TA|BGIBMGA004687-PA|IPR000719|Protein kinase,
IPR008271|Serine/threonine protein kinase, active site,
IPR011009|Protein kinase-like, IPR002290|Serine/threonine protein
kinase
(383 letters)
Database: bee
429 sequences; 140,377 total letters
Searching.....................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protei... 58 1e-10
AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II ... 55 9e-10
DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase ... 52 1e-08
DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase ... 52 1e-08
AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. 44 2e-06
AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C prot... 42 7e-06
AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. 38 1e-04
AF388659-4|AAK71996.1| 1308|Apis mellifera NFRKB-like protein pr... 23 5.8
>AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protein
kinase foraging protein.
Length = 678
Score = 58.0 bits (134), Expect = 1e-10
Identities = 65/227 (28%), Positives = 102/227 (44%), Gaps = 32/227 (14%)
Query: 3 LRVKKVIAEGGFAFVFVAQDV-TTGTEYALKRLMAADEQANKNIIQEISILKKLSGHPNI 61
LR + GGF V + Q + +ALK++ A + Q I K++ G +
Sbjct: 367 LRPLATLGVGGFGRVELVQIAGDSSRSFALKQMKKAQIVETRQQ-QHIMSEKRIMGEAD- 424
Query: 62 IKYIAASFIDKTKTSHGMGEYL-LLTDLCSGGSLMEALQNRGQAFPLPTILRVFYQTC-- 118
F+ K + +YL +L + C GG L L+++G F T FY C
Sbjct: 425 -----CDFVVKLFKTFKDRKYLYMLMEACLGGELWTVLRDKGH-FDDGTTR--FYTACVV 476
Query: 119 RAVQHMHAQVPPIAHRDLKLENFLISNEGTMKLCDFGSATTDVYSPNPSWSANQRNMLEE 178
A ++H++ I +RDLK EN L+ ++G +KL DFG A + +W+
Sbjct: 477 EAFDYLHSR--NIIYRDLKPENLLLDSQGYVKLVDFGFAKR-LDHGRKTWT--------- 524
Query: 179 NLALFTTPMYRAPEMLDTWDNRKIDHSVDMWALGCILYTLCYMQHPF 225
TP Y APE++ N+ D S D W+LG +++ L PF
Sbjct: 525 ---FCGTPEYVAPEVI---LNKGHDISADYWSLGVLMFELLTGTPPF 565
>AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II
protein.
Length = 190
Score = 55.2 bits (127), Expect = 9e-10
Identities = 55/167 (32%), Positives = 77/167 (46%), Gaps = 26/167 (15%)
Query: 116 QTCRAVQHMHAQVPPIAHRDLKLENFLISNEG---TMKLCDFGSATTDVYSPNPSWSANQ 172
Q +V H H + HRDLK EN L++++ +KL DFG A +V +W
Sbjct: 17 QILESVHHCHHN--GVVHRDLKPENLLLASKAKGAAVKLADFGLAI-EVQGEAQAW---- 69
Query: 173 RNMLEENLALFTTPMYRAPEMLDTWDNRKIDHSVDMWALGCILYTLCYMQHPF--EDSAK 230
TP Y +PE+L K VD+WA G ILY L PF ED +
Sbjct: 70 -------FGFAGTPGYLSPEVLKKEPYGK---PVDIWACGVILYILLVGYPPFWDEDQHR 119
Query: 231 L--AILNGNYNL-NPN-DQRYKCFHEIISGCLTVNPADRMTITNVLE 273
L I G+Y+ +P D +I+ LTVNP+ R+T + L+
Sbjct: 120 LYAQIKTGSYDYPSPEWDTVTPEAKNLINQMLTVNPSKRITASEALK 166
>DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase
isoform B protein.
Length = 931
Score = 51.6 bits (118), Expect = 1e-08
Identities = 52/191 (27%), Positives = 90/191 (47%), Gaps = 26/191 (13%)
Query: 30 ALKRLMAADEQANKNIIQEISILKKLSGHPNIIKYIAASFIDKTKTS-HGMGEYLLLTDL 88
A+K ++ +DE ++ E + + H I+K + S ID + G G +LL
Sbjct: 622 AIKSVVPSDESHWNDLAMEFYYNRSIPDHKRIVK-LRGSIIDHSYGGGFGFGSAVLLISD 680
Query: 89 CSGGSLMEALQNRGQAFPLPTILRVFYQTCRAVQHMHAQVPPIAHRDLKLENFLISNEGT 148
L ++ G ++ L I ++ ++++H+Q + HRD+KL+N L+ E
Sbjct: 681 RLSRDLYCGIR-AGLSW-LERI-QIALDVLEGIRYLHSQ--GLVHRDVKLKNVLLDIENR 735
Query: 149 MKLCDFGSATTDVYSPNPSWSANQRNMLEENLALFTTPMYRAPEMLDTWDNRKIDHSVDM 208
KL DFG T+V ML ++ TP++ APE+L + D SVD+
Sbjct: 736 AKLTDFGFCITEVM------------MLG---SIVGTPVHMAPELL----SGHYDSSVDV 776
Query: 209 WALGCILYTLC 219
+A G + + LC
Sbjct: 777 YAFGILFWYLC 787
>DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase
isoform A protein.
Length = 969
Score = 51.6 bits (118), Expect = 1e-08
Identities = 52/191 (27%), Positives = 90/191 (47%), Gaps = 26/191 (13%)
Query: 30 ALKRLMAADEQANKNIIQEISILKKLSGHPNIIKYIAASFIDKTKTS-HGMGEYLLLTDL 88
A+K ++ +DE ++ E + + H I+K + S ID + G G +LL
Sbjct: 660 AIKSVVPSDESHWNDLAMEFYYNRSIPDHKRIVK-LRGSIIDHSYGGGFGFGSAVLLISD 718
Query: 89 CSGGSLMEALQNRGQAFPLPTILRVFYQTCRAVQHMHAQVPPIAHRDLKLENFLISNEGT 148
L ++ G ++ L I ++ ++++H+Q + HRD+KL+N L+ E
Sbjct: 719 RLSRDLYCGIR-AGLSW-LERI-QIALDVLEGIRYLHSQ--GLVHRDVKLKNVLLDIENR 773
Query: 149 MKLCDFGSATTDVYSPNPSWSANQRNMLEENLALFTTPMYRAPEMLDTWDNRKIDHSVDM 208
KL DFG T+V ML ++ TP++ APE+L + D SVD+
Sbjct: 774 AKLTDFGFCITEVM------------MLG---SIVGTPVHMAPELL----SGHYDSSVDV 814
Query: 209 WALGCILYTLC 219
+A G + + LC
Sbjct: 815 YAFGILFWYLC 825
>AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein.
Length = 316
Score = 44.0 bits (99), Expect = 2e-06
Identities = 57/217 (26%), Positives = 91/217 (41%), Gaps = 35/217 (16%)
Query: 9 IAEGGFAFVFVAQDVTTGTEYALKRLMAADEQANKNIIQEISILKKLSGHPNIIKYIAAS 68
+ GGF V+ A + G + A K + N + S LK H NI+K +
Sbjct: 73 LGSGGFGIVYKA--LYKGEQVAAKIIQTEKYSNMLNSEKHASFLK----HSNIVKVLMIE 126
Query: 69 FIDKTKTSHGMGEYLLLTDLCSGGSLMEALQNRGQAFPLPTILRVFYQTCRAVQHMHAQV 128
G L+ +LC G+ ++ + I + TC A+Q H
Sbjct: 127 --------QGASLSLITMELC--GTTLQNRLDEAILIKNERICILKSITC-ALQFCHNA- 174
Query: 129 PPIAHRDLKLENFLISNEGTMKLCDFGSATTDVYSPNPSWSANQRNMLEENLALFTTPMY 188
I H D+K +N L+S G KL DFGS+ + +PN E + TP Y
Sbjct: 175 -GIVHADVKPKNILMSKNGQPKLTDFGSSVL-IGAPN------------EIDKFYGTPGY 220
Query: 189 RAPEMLDTWDNRKIDHSVDMWALGCILYTLCYMQHPF 225
APE++ NR + D+++LG + + + + + PF
Sbjct: 221 TAPEVIK--QNRPTP-AADIYSLGIVAWQMLFRKLPF 254
>AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C
protein.
Length = 149
Score = 42.3 bits (95), Expect = 7e-06
Identities = 26/77 (33%), Positives = 40/77 (51%), Gaps = 3/77 (3%)
Query: 79 MGEYLLLTDLCSGGSLMEALQNRGQAFPLPTILRVFYQTCRAVQHMHAQVPPIAHRDLKL 138
M + + +GG LM +Q G+ F P + VFY + A+ I +RDLKL
Sbjct: 57 MDRLYFVMEYVNGGDLMYQIQQCGK-FKEP--VAVFYASEIAIGLFFLHGRGIVYRDLKL 113
Query: 139 ENFLISNEGTMKLCDFG 155
+N L+ +G +K+ DFG
Sbjct: 114 DNVLLDQDGHIKIADFG 130
>AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein.
Length = 996
Score = 37.9 bits (84), Expect = 1e-04
Identities = 51/259 (19%), Positives = 106/259 (40%), Gaps = 28/259 (10%)
Query: 22 DVTTGTEYALKRLMAAD-EQANKNIIQEISILKKLSGHPNIIKYIAASFIDKTKTSHGMG 80
D T + A+K L ++A + + E SI+ + HPN+I F+ T
Sbjct: 657 DGRTEIDVAIKTLKPGSADKARNDFLTEASIMGQFE-HPNVI------FLQGVVTKSN-- 707
Query: 81 EYLLLTDLCSGGSLMEALQNRGQAFPLPTILRVFYQTCRAVQHMHAQVPPIAHRDLKLEN 140
+++T+ GSL L+ F + ++ + +Q++ A++ + HRDL N
Sbjct: 708 PVMIITEFMENGSLDTFLRANDGKFQVLQLVGMLRGIASGMQYL-AEMNYV-HRDLAARN 765
Query: 141 FLISNEGTMKLCDFGSATTDVYSPNPSWSANQRNMLEENLALFTTPMYRAPEMLDTWDNR 200
L++ K+ DFG + ++ S + + + APE + R
Sbjct: 766 VLVNAALVCKIADFG-LSREIESATEGAYTTRGGKIPVR--------WTAPEAIAF---R 813
Query: 201 KIDHSVDMWALGCILY-TLCYMQHPFEDSAKLAILNG---NYNLNPNDQRYKCFHEIISG 256
K + D+W++G + + + Y + P+ + + ++ Y L + ++++
Sbjct: 814 KFTSASDVWSMGIVCWEVMSYGERPYWNWSNQDVIKSIEKGYRLPAPMDCPEAIYQLMLD 873
Query: 257 CLTVNPADRMTITNVLERL 275
C R T N+ + L
Sbjct: 874 CWQKERTHRPTFANLTQTL 892
>AF388659-4|AAK71996.1| 1308|Apis mellifera NFRKB-like protein
protein.
Length = 1308
Score = 22.6 bits (46), Expect = 5.8
Identities = 11/59 (18%), Positives = 25/59 (42%)
Query: 251 HEIISGCLTVNPADRMTITNVLERLAAIAETQNVNLKQPLKFERKKVEQSVATSSPANR 309
H ++ +V + +TNV L I V +Q + +++ +Q ++ A +
Sbjct: 939 HSVLHSAQSVVASSASNVTNVTTNLTTILPPVKVQSQQQSQQSQQQQQQQTIVTNQAGK 997
Database: bee
Posted date: Oct 5, 2007 11:13 AM
Number of letters in database: 140,377
Number of sequences in database: 429
Lambda K H
0.318 0.132 0.385
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 97,625
Number of Sequences: 429
Number of extensions: 3873
Number of successful extensions: 16
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 3
Number of HSP's gapped (non-prelim): 11
length of query: 383
length of database: 140,377
effective HSP length: 59
effective length of query: 324
effective length of database: 115,066
effective search space: 37281384
effective search space used: 37281384
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 45 (22.2 bits)
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