Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA004684-TA|BGIBMGA004684-PA|IPR002018|Carboxylesterase,
type B, IPR002168|Lipolytic enzyme
(539 letters)
Database: bee
429 sequences; 140,377 total letters
Searching.....................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY647436-1|AAU81605.1| 567|Apis mellifera juvenile hormone este... 262 4e-72
AB083009-1|BAC54130.1| 567|Apis mellifera esterase protein. 262 4e-72
AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase ... 178 8e-47
AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase ... 156 5e-40
AY526235-1|AAS20468.1| 169|Apis mellifera esterase protein. 128 2e-31
AF213011-1|AAG43567.1| 62|Apis mellifera esterase A2 protein. 53 5e-09
AY601637-1|AAT11850.1| 683|Apis mellifera hexamerin 70b protein. 24 2.8
>AY647436-1|AAU81605.1| 567|Apis mellifera juvenile hormone
esterase protein.
Length = 567
Score = 262 bits (643), Expect = 4e-72
Identities = 169/541 (31%), Positives = 265/541 (48%), Gaps = 29/541 (5%)
Query: 3 DNPLVTVEQGTLRGSKKTLLDGSPYYSFKGIPYAEPPIGELRFQAPKPRKPWNGVLDAVE 62
D P V G ++G K +G Y +++GIPYA PP+G+ RF+AP+ W G L A +
Sbjct: 21 DAPRVKTPLGAIKGYYKISGNGKQYEAYEGIPYALPPVGKFRFKAPQKIPAWIGELSATK 80
Query: 63 HGPVCPQYD---ISTSSTIEGNEDCLYLNVYTRSLQSDSK-IPVMVYIHXXXXXXXXXXX 118
G C QY ++ IEG EDCLYLNVY + ++ S+ +PV+ +IH
Sbjct: 81 FGFPCLQYTQLPVNPRDKIEGAEDCLYLNVYVPADRTPSQSLPVIFWIHGGAFQFGSGIP 140
Query: 119 XXXXPEFIIQHNVVLVTINYRLELLGFLSLDTAEVPGNAGMKDQVAALRWVKGNIVRFGG 178
++++ +V+ VTINYRL +LGFLS + VPGN G+KDQ ALRWV NI FGG
Sbjct: 141 MGA--KYLMDSDVIFVTINYRLGILGFLSTEDEVVPGNMGLKDQSMALRWVSENIEWFGG 198
Query: 179 DPDNVTIFGESAGAASVTYHMLSPMSKGLFHKVIAQSGVCINDWAIGNKTLERVYRVANL 238
+P +T+ G SAG ASV YH LSP+S GLF I+ SG +N W +LE+ +V
Sbjct: 199 NPKRITLIGLSAGGASVHYHYLSPLSAGLFQGGISISGTALNCWTQTENSLEKAKQVGAF 258
Query: 239 LGKECSDVQDLLEFLRSVDPLKLTCVTYRTRTQDEKYRGLPMYFVPIVEKKFDGVEA--F 296
+G +V++++ LR P + T + P F P+ EK + + F
Sbjct: 259 MGCPTRNVKEMIRCLR-YRPARAIVETLANFMRFYYNPFTP--FGPVTEKVNNDSNSLPF 315
Query: 297 LSEEPIETLMSGNINNVPILLGYNKSEGVLMVADVLKKKE---IMNEKPSFIVP--REIA 351
+ PIE + SG++ +VP + G EG+ VA+ + K E +++E I P +
Sbjct: 316 IDRTPIEIINSGDVQDVPWVTGVTSEEGLYPVAEFIAKPEALKLLDENWDLIAPYFLDYN 375
Query: 352 QTVSEEKIKEFGERIKRFYFGDEGISADNTATIVDFQTDLYFAYNVHRFAYLYSVYN-YP 410
T+ +EK E I+ +YF I ++D +D +F + + A + + N P
Sbjct: 376 YTIPKEKHVEVARLIRNYYFESNKIDETTLKHLIDVASDRFFITDGEKAARMQAKVNRQP 435
Query: 411 IYLYRFDYETELNFIKNALGQGGIKGAAHADDLFYFFYNPI--NKHLIGDERLKIIIYEV 468
++ Y + Y+ + + G G HADD + P + D +++ ++ +
Sbjct: 436 VWFYYYTYKGAHSISEIMSGTSNKYGVCHADDAYMVVDTPFLASTTTTNDIKMQKVLID- 494
Query: 469 TKLWTDFSKTGNPTPDNALGVKWYPYSVNGNKIMFL----DEPLRFEQCFEIDRVDFWNK 524
W F G P N V+W + N + +L ++ + R DFWN
Sbjct: 495 --FWVSFVNNGVP---NVNSVQWPRLNPNEKSLHYLHIAGPGKIQMDSSTNFGREDFWNS 549
Query: 525 L 525
+
Sbjct: 550 I 550
>AB083009-1|BAC54130.1| 567|Apis mellifera esterase protein.
Length = 567
Score = 262 bits (643), Expect = 4e-72
Identities = 169/541 (31%), Positives = 265/541 (48%), Gaps = 29/541 (5%)
Query: 3 DNPLVTVEQGTLRGSKKTLLDGSPYYSFKGIPYAEPPIGELRFQAPKPRKPWNGVLDAVE 62
D P V G ++G K +G Y +++GIPYA PP+G+ RF+AP+ W G L A +
Sbjct: 21 DAPRVKTPLGAIKGYYKISGNGKQYEAYEGIPYALPPVGKFRFKAPQKIPAWIGELSATK 80
Query: 63 HGPVCPQYD---ISTSSTIEGNEDCLYLNVYTRSLQSDSK-IPVMVYIHXXXXXXXXXXX 118
G C QY ++ IEG EDCLYLNVY + ++ S+ +PV+ +IH
Sbjct: 81 FGFPCLQYTQLPVNPRDKIEGAEDCLYLNVYVPADRTPSQSLPVIFWIHGGAFQFGSGIP 140
Query: 119 XXXXPEFIIQHNVVLVTINYRLELLGFLSLDTAEVPGNAGMKDQVAALRWVKGNIVRFGG 178
++++ +V+ VTINYRL +LGFLS + VPGN G+KDQ ALRWV NI FGG
Sbjct: 141 MGA--KYLMDSDVIFVTINYRLGILGFLSTEDEVVPGNMGLKDQSMALRWVSENIEWFGG 198
Query: 179 DPDNVTIFGESAGAASVTYHMLSPMSKGLFHKVIAQSGVCINDWAIGNKTLERVYRVANL 238
+P +T+ G SAG ASV YH LSP+S GLF I+ SG +N W +LE+ +V
Sbjct: 199 NPKRITLIGLSAGGASVHYHYLSPLSAGLFQGGISISGTALNCWTQTENSLEKAKQVGAF 258
Query: 239 LGKECSDVQDLLEFLRSVDPLKLTCVTYRTRTQDEKYRGLPMYFVPIVEKKFDGVEA--F 296
+G +V++++ LR P + T + P F P+ EK + + F
Sbjct: 259 MGCPTRNVKEMIRCLR-YRPARAIVETLANFMRFYYNPFTP--FGPVTEKVNNDSNSLPF 315
Query: 297 LSEEPIETLMSGNINNVPILLGYNKSEGVLMVADVLKKKE---IMNEKPSFIVP--REIA 351
+ PIE + SG++ +VP + G EG+ VA+ + K E +++E I P +
Sbjct: 316 IDRTPIEIINSGDVQDVPWVTGVTSEEGLYPVAEFIAKPEALKLLDENWDLIAPYFLDYN 375
Query: 352 QTVSEEKIKEFGERIKRFYFGDEGISADNTATIVDFQTDLYFAYNVHRFAYLYSVYN-YP 410
T+ +EK E I+ +YF I ++D +D +F + + A + + N P
Sbjct: 376 YTIPKEKHVEVARLIRNYYFESNKIDETTLKHLIDVASDRFFITDGEKAARMQAKVNRQP 435
Query: 411 IYLYRFDYETELNFIKNALGQGGIKGAAHADDLFYFFYNPI--NKHLIGDERLKIIIYEV 468
++ Y + Y+ + + G G HADD + P + D +++ ++ +
Sbjct: 436 VWFYYYTYKGAHSISEIMSGTSNKYGVCHADDAYMVVDTPFLASTTTTNDIKMQKVLID- 494
Query: 469 TKLWTDFSKTGNPTPDNALGVKWYPYSVNGNKIMFL----DEPLRFEQCFEIDRVDFWNK 524
W F G P N V+W + N + +L ++ + R DFWN
Sbjct: 495 --FWVSFVNNGVP---NVNSVQWPRLNPNEKSLHYLHIAGPGKIQMDSSTNFGREDFWNS 549
Query: 525 L 525
+
Sbjct: 550 I 550
>AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase
protein.
Length = 628
Score = 178 bits (434), Expect = 8e-47
Identities = 152/545 (27%), Positives = 252/545 (46%), Gaps = 62/545 (11%)
Query: 3 DNPLVT-VEQGTLRGSKKTLLDGSPYYSFKGIPYAEPPIGELRFQAPKPRKPWNGVLDAV 61
++PLV G +RG +T+LD + F GIP+A+PPIG LRF+ P P +PW+GVL+A
Sbjct: 35 NDPLVVETTSGLVRGFPRTVLD-KEVHVFYGIPFAKPPIGPLRFRKPLPIEPWHGVLNAT 93
Query: 62 -----------EHGPVCPQYDISTSSTIEGNEDCLYLNV-----YTRSLQSDSK------ 99
E+ P P ++ +T +EDCLYLN+ Y + D
Sbjct: 94 VLPNSCYQERYEYFPGFPGEEMWNPNT-NISEDCLYLNIWVPQKYRLRHKGDGSPGGNGG 152
Query: 100 -----IPVMVYIHXXXXXXXXXXXXXXXPEFIIQ-HNVVLVTINYRLELLGFLSLD---- 149
+P++V+I+ + + NV++ ++ YR+ GFL L+
Sbjct: 153 PRNGLLPLLVWIYGGGFMSGTATLDVYNADIMAATSNVIIASMQYRVGAFGFLYLNKHFT 212
Query: 150 -TAEVPGNAGMKDQVAALRWVKGNIVRFGGDPDNVTIFGESAGAASVTYHMLSPMSKGLF 208
+ E PGN G+ DQ ALRW++ N FGGDP+ +TIFGESAG +SV+ H++SP+++GL
Sbjct: 213 NSEEAPGNMGLWDQALALRWLRDNAEAFGGDPELITIFGESAGGSSVSLHLISPVTRGLV 272
Query: 209 HKVIAQSGVCINDWAIGNKTLERVYRVANLLGKECSDVQDLLEFLRSVDPLKLTCVTYRT 268
+ I QSG W+ + E+ VA +L +C +L + + V +T
Sbjct: 273 RRGILQSGTLNAPWSY--MSGEKANEVATILVDDCGCNSTMLNENPARVMACMRSVDAKT 330
Query: 269 RT--QDEKYRGLPMYFVPIVEKKFDGVEAFLSEEPIETLMSGNINNVPILLGYNKSEGVL 326
+ Q Y G+ + P DG+ FL + P++ L + + IL+G N++EG
Sbjct: 331 ISVQQWNSYWGILGF--PSA-PTIDGI--FLPKHPLDLLREADFKDTEILIGNNENEGTY 385
Query: 327 MVADVLKKKEIMNEKPSFIVPRE----IAQTVSEEKIKEFGERIKRFYFGDEGI--SADN 380
+ + +I + + + RE I + + + E I Y E +
Sbjct: 386 FI--LYDFNDIFEKDQASFLERERFLGIINNIFKNMSQIEREAITFQYTDWEEVYNGYIY 443
Query: 381 TATIVDFQTDLYFAYNVHRFAYLYSVYNYPIYLYRFDYETELNFIKNALGQGGIKGAAHA 440
+ D D +F FA L++ +Y Y F T N G G H
Sbjct: 444 QKMVADVVGDYFFICPSIHFAQLFADRGMKVYYYFFTQRTSTNL------WGEWMGVLHG 497
Query: 441 DDLFYFFYNPINKHLIGDERLKIIIYEVTKLWTDFSKTGNPTPDNALGVKWYPYSVNGNK 500
D++ Y F +P+NK L ++ + + + +++F+ G PT +++ +W YS + K
Sbjct: 498 DEVEYVFGHPLNKSLKYSDKERDLSLRMILYFSEFAYLGKPTKEDS---EWPSYSRDEPK 554
Query: 501 IMFLD 505
D
Sbjct: 555 YFIFD 559
>AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase
protein.
Length = 492
Score = 156 bits (378), Expect = 5e-40
Identities = 133/463 (28%), Positives = 215/463 (46%), Gaps = 53/463 (11%)
Query: 3 DNPLVT-VEQGTLRGSKKTLLDGSPYYSFKGIPYAEPPIGELRFQAPKPRKPWNGVLDAV 61
++PLV G +RG +T+LD + F GIP+A+PPIG LRF+ P P +PW+GVL+A
Sbjct: 35 NDPLVVETTSGLVRGFPRTVLD-KEVHVFYGIPFAKPPIGPLRFRKPLPIEPWHGVLNAT 93
Query: 62 -----------EHGPVCPQYDISTSSTIEGNEDCLYLNV-----YTRSLQSDSK------ 99
E+ P P ++ +T +EDCLYLN+ Y + D
Sbjct: 94 VLPNSCYQERYEYFPGFPGEEMWNPNT-NISEDCLYLNIWVPQKYRLRHKGDGSPGGNGG 152
Query: 100 -----IPVMVYIHXXXXXXXXXXXXXXXPEFIIQ-HNVVLVTINYRLELLGFLSLD---- 149
+P++V+I+ + + NV++ ++ YR+ GFL L+
Sbjct: 153 PRNGLLPLLVWIYGGGFMSGTATLDVYNADIMAATSNVIIASMQYRVGAFGFLYLNKHFT 212
Query: 150 -TAEVPGNAGMKDQVAALRWVKGNIVRFGGDPDNVTIFGESAGAASVTYHMLSPMSKGLF 208
+ E PGN G+ DQ ALRW++ N FGGDP+ +TIFGESAG +SV+ H++SP+++GL
Sbjct: 213 NSEEAPGNMGLWDQALALRWLRDNAEAFGGDPELITIFGESAGGSSVSLHLISPVTRGLV 272
Query: 209 HKVIAQSGVCINDWAIGNKTLERVYRVANLLGKECSDVQDLLEFLRSVDPLKLTCVTYRT 268
+ I QSG W+ + E+ VA +L +C +L + + V +T
Sbjct: 273 RRGILQSGTLNAPWSY--MSGEKANEVATILVDDCGCNSTMLNENPARVMACMRSVDAKT 330
Query: 269 RT--QDEKYRGLPMYFVPIVEKKFDGVEAFLSEEPIETLMSGNINNVPILLGYNKSEGVL 326
+ Q Y G+ + P DG+ FL + P++ L + + IL+G N++EG
Sbjct: 331 ISVQQWNSYWGILGF--PSA-PTIDGI--FLPKHPLDLLREADFKDTEILIGNNENEGTY 385
Query: 327 MVADVLKKKEIMNEKPSFIVPRE----IAQTVSEEKIKEFGERIKRFYFGDEGI--SADN 380
+ + +I + + + RE I + + + E I Y E +
Sbjct: 386 FI--LYDFNDIFEKDQASFLERERFLGIINNIFKNMSQIEREAITFQYTDWEEVYNGYIY 443
Query: 381 TATIVDFQTDLYFAYNVHRFAYLYSVYNYPIYLYRFDYETELN 423
+ D D +F FA L++ +Y Y F T N
Sbjct: 444 QKMVADVVGDYFFICPSIHFAQLFADRGMKVYYYFFTQRTSTN 486
>AY526235-1|AAS20468.1| 169|Apis mellifera esterase protein.
Length = 169
Score = 128 bits (308), Expect = 2e-31
Identities = 61/131 (46%), Positives = 85/131 (64%)
Query: 124 EFIIQHNVVLVTINYRLELLGFLSLDTAEVPGNAGMKDQVAALRWVKGNIVRFGGDPDNV 183
++++ +V+ VTINYRL +LGFLS + VPGN G+KDQ ALRWV NI FGG+P +
Sbjct: 15 KYLMDSDVIFVTINYRLGILGFLSTEDEVVPGNMGLKDQSMALRWVSENIEWFGGNPKRI 74
Query: 184 TIFGESAGAASVTYHMLSPMSKGLFHKVIAQSGVCINDWAIGNKTLERVYRVANLLGKEC 243
T+ G SAG ASV YH LSP+S GLF I+ SG +N W +LE+ +V +G
Sbjct: 75 TLIGLSAGGASVHYHYLSPLSAGLFQGGISISGTALNCWTQTENSLEKAKQVGAFMGCPT 134
Query: 244 SDVQDLLEFLR 254
+V++++ LR
Sbjct: 135 RNVKEMIRCLR 145
>AF213011-1|AAG43567.1| 62|Apis mellifera esterase A2 protein.
Length = 62
Score = 53.2 bits (122), Expect = 5e-09
Identities = 25/63 (39%), Positives = 35/63 (55%), Gaps = 2/63 (3%)
Query: 82 EDCLYLNVYTRSLQSDSKIPVMVYIHXXXXXXXXXXXXXXXPEFIIQHNVVLVTINYRLE 141
EDCLYL+VYT SL D PVM Y+H P++++ +VV+V+ NYR+
Sbjct: 2 EDCLYLDVYTNSL--DQSKPVMFYVHEGAFISGTSSFHEMRPDYLLPKDVVVVSSNYRVG 59
Query: 142 LLG 144
G
Sbjct: 60 AFG 62
>AY601637-1|AAT11850.1| 683|Apis mellifera hexamerin 70b protein.
Length = 683
Score = 24.2 bits (50), Expect = 2.8
Identities = 10/20 (50%), Positives = 14/20 (70%)
Query: 169 VKGNIVRFGGDPDNVTIFGE 188
V GNIV+ GD NV ++G+
Sbjct: 360 VLGNIVQGNGDSVNVQLYGQ 379
Database: bee
Posted date: Oct 5, 2007 11:13 AM
Number of letters in database: 140,377
Number of sequences in database: 429
Lambda K H
0.320 0.140 0.422
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 163,022
Number of Sequences: 429
Number of extensions: 7969
Number of successful extensions: 39
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 11
Number of HSP's gapped (non-prelim): 13
length of query: 539
length of database: 140,377
effective HSP length: 61
effective length of query: 478
effective length of database: 114,208
effective search space: 54591424
effective search space used: 54591424
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 46 (22.6 bits)
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