Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA004358-TA|BGIBMGA004358-PA|IPR000299|Band 4.1,
IPR008266|Tyrosine protein kinase, active site, IPR000719|Protein
kinase, IPR011009|Protein kinase-like, IPR001245|Tyrosine protein
kinase
(579 letters)
Database: bee
429 sequences; 140,377 total letters
Searching.....................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein. 184 1e-48
AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protei... 60 5e-11
AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein. 53 7e-09
DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase ... 50 7e-08
DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase ... 50 7e-08
AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II ... 47 4e-07
AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C prot... 45 2e-06
AB194707-1|BAD69622.1| 247|Apis mellifera heme oxygenase protein. 23 5.2
>AY921579-1|AAX14899.1| 996|Apis mellifera ephrin receptor protein.
Length = 996
Score = 184 bits (449), Expect = 1e-48
Identities = 110/303 (36%), Positives = 159/303 (52%), Gaps = 27/303 (8%)
Query: 283 DDADYSTP--AVRDY--ELVRNQIELTGIIGEGQFGDVHKGTCRVTSANHPSLRRQLSIX 338
D Y P AVR++ E+ I + IIG G+FGDV +G ++ P R ++
Sbjct: 610 DPHTYEDPNQAVREFAREIDAGYITIEAIIGGGEFGDVCRGKLKLP----PDGRTEID-- 663
Query: 339 XXXXXXXXXEYVLPVAVKTCKMDADLDTAEKFLEEAYIMQQFSHPHIIGLVGVCS-SSPI 397
VA+KT K + FL EA IM QF HP++I L GV + S+P+
Sbjct: 664 --------------VAIKTLKPGSADKARNDFLTEASIMGQFEHPNVIFLQGVVTKSNPV 709
Query: 398 WIVMELATLGEMRAYLQQNAHRLETCQLVLYIYQLSTALSYLESKKFVHRDIAARNVLVS 457
I+ E G + +L+ N + + QLV + +++ + YL +VHRD+AARNVLV+
Sbjct: 710 MIITEFMENGSLDTFLRANDGKFQVLQLVGMLRGIASGMQYLAEMNYVHRDLAARNVLVN 769
Query: 458 TPTCVKLADFGLSKMVED--KSYYKASRGKLPIKWMAPESINFRRFTSASDVWMFGVCMW 515
K+ADFGLS+ +E + Y GK+P++W APE+I FR+FTSASDVW G+ W
Sbjct: 770 AALVCKIADFGLSREIESATEGAYTTRGGKIPVRWTAPEAIAFRKFTSASDVWSMGIVCW 829
Query: 516 EILMLGVKPFSGVKNNDVIGKLENGEXXXXXXXXXXXXYSVMSRCWAYEPSHRPAAHLLK 575
E++ G +P+ N DVI +E G Y +M CW E +HRP L
Sbjct: 830 EVMSYGERPYWNWSNQDVIKSIEKGYRLPAPMDCPEAIYQLMLDCWQKERTHRPTFANLT 889
Query: 576 ETL 578
+TL
Sbjct: 890 QTL 892
>AF469010-1|AAL93136.1| 678|Apis mellifera cGMP-dependent protein
kinase foraging protein.
Length = 678
Score = 60.1 bits (139), Expect = 5e-11
Identities = 39/131 (29%), Positives = 66/131 (50%), Gaps = 4/131 (3%)
Query: 397 IWIVMELATLGEMRAYLQQNAHRLETCQLVLYIYQLSTALSYLESKKFVHRDIAARNVLV 456
++++ME GE+ L+ H + Y + A YL S+ ++RD+ N+L+
Sbjct: 441 LYMLMEACLGGELWTVLRDKGH-FDDGTTRFYTACVVEAFDYLHSRNIIYRDLKPENLLL 499
Query: 457 STPTCVKLADFGLSKMVEDKSYYKASRGKLPIKWMAPESINFRRFTSASDVWMFGVCMWE 516
+ VKL DFG +K ++ G P +++APE I + ++D W GV M+E
Sbjct: 500 DSQGYVKLVDFGFAKRLDHGRKTWTFCG-TP-EYVAPEVILNKGHDISADYWSLGVLMFE 557
Query: 517 ILMLGVKPFSG 527
L+ G PF+G
Sbjct: 558 -LLTGTPPFTG 567
>AB183889-1|BAD86829.1| 316|Apis mellifera Mos protein.
Length = 316
Score = 52.8 bits (121), Expect = 7e-09
Identities = 31/109 (28%), Positives = 57/109 (52%), Gaps = 3/109 (2%)
Query: 426 VLYIYQLSTALSYLESKKFVHRDIAARNVLVSTPTCVKLADFGLSKMVEDKSYYKASRGK 485
+ + ++ AL + + VH D+ +N+L+S KL DFG S ++ + G
Sbjct: 158 ICILKSITCALQFCHNAGIVHADVKPKNILMSKNGQPKLTDFGSSVLIGAPNEIDKFYG- 216
Query: 486 LPIKWMAPESINFRRFTSASDVWMFGVCMWEILMLGVKPFSGVKNNDVI 534
P + APE I R T A+D++ G+ W++L + PF+G+ ++ +I
Sbjct: 217 TP-GYTAPEVIKQNRPTPAADIYSLGIVAWQMLFRKL-PFAGLHSHTII 263
>DQ013068-1|AAY81956.1| 931|Apis mellifera dusty protein kinase
isoform B protein.
Length = 931
Score = 49.6 bits (113), Expect = 7e-08
Identities = 43/159 (27%), Positives = 71/159 (44%), Gaps = 17/159 (10%)
Query: 420 LETCQLVLYIYQLSTALSYLESKKFVHRDIAARNVLVSTPTCVKLADFGLSKMVEDKSYY 479
LE Q+ L + + + YL S+ VHRD+ +NVL+ KL DFG + +
Sbjct: 697 LERIQIALDVLE---GIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGF--CITEVMML 751
Query: 480 KASRGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMLGVK---PFSGVKNNDVI-- 534
+ G P+ MAPE ++ + S+ DV+ FG+ W + V+ F N +++
Sbjct: 752 GSIVG-TPVH-MAPELLS-GHYDSSVDVYAFGILFWYLCAGHVRLPYTFEQFHNKELLWT 808
Query: 535 ----GKLENGEXXXXXXXXXXXXYSVMSRCWAYEPSHRP 569
+ G + +M +CW+ EPS RP
Sbjct: 809 SVKKALMIVGIRPERLPSFDDECWRLMEQCWSGEPSKRP 847
>DQ013067-1|AAY81955.1| 969|Apis mellifera dusty protein kinase
isoform A protein.
Length = 969
Score = 49.6 bits (113), Expect = 7e-08
Identities = 43/159 (27%), Positives = 71/159 (44%), Gaps = 17/159 (10%)
Query: 420 LETCQLVLYIYQLSTALSYLESKKFVHRDIAARNVLVSTPTCVKLADFGLSKMVEDKSYY 479
LE Q+ L + + + YL S+ VHRD+ +NVL+ KL DFG + +
Sbjct: 735 LERIQIALDVLE---GIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGF--CITEVMML 789
Query: 480 KASRGKLPIKWMAPESINFRRFTSASDVWMFGVCMWEILMLGVK---PFSGVKNNDVI-- 534
+ G P+ MAPE ++ + S+ DV+ FG+ W + V+ F N +++
Sbjct: 790 GSIVG-TPVH-MAPELLS-GHYDSSVDVYAFGILFWYLCAGHVRLPYTFEQFHNKELLWT 846
Query: 535 ----GKLENGEXXXXXXXXXXXXYSVMSRCWAYEPSHRP 569
+ G + +M +CW+ EPS RP
Sbjct: 847 SVKKALMIVGIRPERLPSFDDECWRLMEQCWSGEPSKRP 885
>AB013287-1|BAA87893.1| 190|Apis mellifera calmodulin kinase II
protein.
Length = 190
Score = 47.2 bits (107), Expect = 4e-07
Identities = 30/115 (26%), Positives = 58/115 (50%), Gaps = 5/115 (4%)
Query: 429 IYQLSTALSYLESKKFVHRDIAARNVLVSTPT---CVKLADFGLSKMVEDKSYYKASRGK 485
I Q+ ++ + VHRD+ N+L+++ VKLADFGL+ V+ ++
Sbjct: 15 IQQILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGEAQAWFGFAG 74
Query: 486 LPIKWMAPESINFRRFTSASDVWMFGVCMWEILMLGVKPFSGVKNNDVIGKLENG 540
P +++PE + + D+W GV ++ IL++G PF + + +++ G
Sbjct: 75 TP-GYLSPEVLKKEPYGKPVDIWACGVILY-ILLVGYPPFWDEDQHRLYAQIKTG 127
>AB013288-1|BAA87894.1| 149|Apis mellifera protein kinase C
protein.
Length = 149
Score = 44.8 bits (101), Expect = 2e-06
Identities = 31/122 (25%), Positives = 58/122 (47%), Gaps = 5/122 (4%)
Query: 354 AVKTCKMDADL--DTAEKFLEEAYIMQQFSHPHIIGLVGVC--SSSPIWIVMELATLGEM 409
A+K K D + D E + E ++ + P + + C + ++ VME G++
Sbjct: 13 AIKILKKDIIIQDDDVECTMVEKRVLALSTKPPFLVQLHSCFQTMDRLYFVMEYVNGGDL 72
Query: 410 RAYLQQNAHRLETCQLVLYIYQLSTALSYLESKKFVHRDIAARNVLVSTPTCVKLADFGL 469
+QQ + + V Y +++ L +L + V+RD+ NVL+ +K+ADFG+
Sbjct: 73 MYQIQQ-CGKFKEPVAVFYASEIAIGLFFLHGRGIVYRDLKLDNVLLDQDGHIKIADFGM 131
Query: 470 SK 471
K
Sbjct: 132 CK 133
>AB194707-1|BAD69622.1| 247|Apis mellifera heme oxygenase protein.
Length = 247
Score = 23.4 bits (48), Expect = 5.2
Identities = 13/49 (26%), Positives = 21/49 (42%)
Query: 96 LKKAIQQQFKKVANLSDTECMLKYLETMHTHYGYDRETFAGALGTGWFI 144
LK+ ++ K+A D + K +E T + + E GTG I
Sbjct: 174 LKQHMRDTMNKIAETLDEDTKNKLIEESKTVFILNNEIIRSIQGTGTII 222
Database: bee
Posted date: Oct 5, 2007 11:13 AM
Number of letters in database: 140,377
Number of sequences in database: 429
Lambda K H
0.319 0.133 0.392
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 138,384
Number of Sequences: 429
Number of extensions: 5029
Number of successful extensions: 24
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 3
Number of HSP's gapped (non-prelim): 13
length of query: 579
length of database: 140,377
effective HSP length: 61
effective length of query: 518
effective length of database: 114,208
effective search space: 59159744
effective search space used: 59159744
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 46 (22.6 bits)
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