GeneModel BLAST Result against bee

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA004216-TA|BGIBMGA004216-
PA|IPR000175|Sodium:neurotransmitter symporter
         (614 letters)

Database: bee 
           429 sequences; 140,377 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY395072-1|AAQ96728.1|  593|Apis mellifera GABA neurotransmitter...   291   1e-80
AY395071-1|AAQ96727.1|  646|Apis mellifera GABA neurotransmitter...   291   1e-80
AY395073-1|AAQ96729.1|  203|Apis mellifera GABA neurotransmitter...   122   9e-30

>AY395072-1|AAQ96728.1|  593|Apis mellifera GABA neurotransmitter
           transporter-1B protein.
          Length = 593

 Score =  291 bits (713), Expect = 1e-80
 Identities = 139/326 (42%), Positives = 205/326 (62%), Gaps = 26/326 (7%)

Query: 51  DRAAWSGKLQFFLSIIGYSVGLGNIWRFPYLCQQNGGGAFLIPFLIMLVLEGVPLFLIEM 110
           DR  WS K++F LS++G ++GLGN+WRFPYLC +NGGGAF++P+ I L L GVP+FL+E+
Sbjct: 18  DRGMWSSKIEFILSVVGLAIGLGNLWRFPYLCYKNGGGAFMVPYFIALALAGVPMFLMEL 77

Query: 111 AIGQKMRLGSLGVWNTIHPWLGGIGISSCIVTLFVALYYNVIITWVFFYLFNSIRLTADQ 170
           ++GQ M +G LGV+  I P   GIG ++C+++ +  +YY +I+ W  FYL  S+R+    
Sbjct: 78  SLGQMMTIGGLGVFK-IAPIFKGIGYATCVLSCWTNIYYIIILAWALFYLLVSLRIDLPW 136

Query: 171 L----PWSS--CPQDNGTAEAEC--------------NKASATVY-----FWYREALDAA 205
                PW++  C       EA C              N + A +      FW R  L  +
Sbjct: 137 RTCGNPWNTRYCLTPTERLEALCWTQDEDVICSTPIGNLSHALLKDPVKEFWERRTLQIS 196

Query: 206 PSIEDPGVPRWWIVLYLLLAWVIVFCIVMKGIQSSGKVVYFTSLFPYVVLTIFFIRGITL 265
             IE+ G  RW +   L + W++ +  + KG++ +GKVVYFTSLFPY +LTI  IRG+TL
Sbjct: 197 DGIENIGSIRWELAGTLAVVWIMCYFCIWKGVKWTGKVVYFTSLFPYALLTILLIRGLTL 256

Query: 266 PGSSDGILHMYKPKLEKLLDPTVWLDAATQVFYSFGLAFGSLIAFGSYNPPNNNCVRDVL 325
           PG+ +G+ +   P L KL DP VW+DA TQ+F+S+ L  G+L+A GSYN  NNN  +D L
Sbjct: 257 PGAMEGLKYYVTPNLSKLSDPEVWIDAVTQIFFSYALGLGALVALGSYNKFNNNVYKDAL 316

Query: 326 LVSVCNALTAIYASVVIFSILGFKAY 351
           +V   N+ T++ + +VIFS++GF A+
Sbjct: 317 IVCTVNSCTSMLSGIVIFSVVGFMAH 342



 Score = 70.9 bits (166), Expect = 3e-14
 Identities = 46/171 (26%), Positives = 76/171 (44%), Gaps = 3/171 (1%)

Query: 436 AAEGTGLAFIVFTQAILKLTPAPXXXXXXXXXXXXXXXXXXXXIMEGMLCTIFDI-DFFK 494
           A  G GLAF+V+  A+L+L  +                      +EG +    D      
Sbjct: 352 AVSGPGLAFLVYPSAVLELPGSSIWSCLFFFMLILIGLDSQFCTVEGFITAAVDEWPRLL 411

Query: 495 RLSKPVITGIVCSFCFLVGLIFTTGAGEYWLKMFDSFAGT-IGLVVVALLEMVSVIYIYG 553
           R  K +   IVC   +L+GL   T  G Y  ++ DS+A +   L+ +   E +++ + +G
Sbjct: 412 RKRKELFIAIVCFVSYLIGLFCITEGGMYVFQLLDSYAVSGFCLLFLMFFECIAISWAFG 471

Query: 554 HERFSNDIYEMTGYRPGLYWQVTWRYVGPFI-VTCILFSSLVFMLINPPTY 603
             RF + I +M GY P  +W++ W    P I V    F+ + F+ +   TY
Sbjct: 472 VNRFYDGIRDMIGYYPCCWWKICWTITTPAICVGVFTFNIIKFVPVKYLTY 522


>AY395071-1|AAQ96727.1|  646|Apis mellifera GABA neurotransmitter
           transporter-1B protein.
          Length = 646

 Score =  291 bits (713), Expect = 1e-80
 Identities = 139/326 (42%), Positives = 205/326 (62%), Gaps = 26/326 (7%)

Query: 51  DRAAWSGKLQFFLSIIGYSVGLGNIWRFPYLCQQNGGGAFLIPFLIMLVLEGVPLFLIEM 110
           DR  WS K++F LS++G ++GLGN+WRFPYLC +NGGGAF++P+ I L L GVP+FL+E+
Sbjct: 71  DRGMWSSKIEFILSVVGLAIGLGNLWRFPYLCYKNGGGAFMVPYFIALALAGVPMFLMEL 130

Query: 111 AIGQKMRLGSLGVWNTIHPWLGGIGISSCIVTLFVALYYNVIITWVFFYLFNSIRLTADQ 170
           ++GQ M +G LGV+  I P   GIG ++C+++ +  +YY +I+ W  FYL  S+R+    
Sbjct: 131 SLGQMMTIGGLGVFK-IAPIFKGIGYATCVLSCWTNIYYIIILAWALFYLLVSLRIDLPW 189

Query: 171 L----PWSS--CPQDNGTAEAEC--------------NKASATVY-----FWYREALDAA 205
                PW++  C       EA C              N + A +      FW R  L  +
Sbjct: 190 RTCGNPWNTRYCLTPTERLEALCWTQDEDIICSTPIGNLSHALLKDPVKEFWERRTLQIS 249

Query: 206 PSIEDPGVPRWWIVLYLLLAWVIVFCIVMKGIQSSGKVVYFTSLFPYVVLTIFFIRGITL 265
             IE+ G  RW +   L + W++ +  + KG++ +GKVVYFTSLFPY +LTI  IRG+TL
Sbjct: 250 DGIENIGSIRWELAGTLAVVWIMCYFCIWKGVKWTGKVVYFTSLFPYALLTILLIRGLTL 309

Query: 266 PGSSDGILHMYKPKLEKLLDPTVWLDAATQVFYSFGLAFGSLIAFGSYNPPNNNCVRDVL 325
           PG+ +G+ +   P L KL DP VW+DA TQ+F+S+ L  G+L+A GSYN  NNN  +D L
Sbjct: 310 PGAMEGLKYYVTPNLSKLSDPEVWIDAVTQIFFSYALGLGALVALGSYNKFNNNVYKDAL 369

Query: 326 LVSVCNALTAIYASVVIFSILGFKAY 351
           +V   N+ T++ + +VIFS++GF A+
Sbjct: 370 IVCTVNSCTSMLSGIVIFSVVGFMAH 395



 Score = 72.5 bits (170), Expect = 9e-15
 Identities = 47/171 (27%), Positives = 77/171 (45%), Gaps = 3/171 (1%)

Query: 436 AAEGTGLAFIVFTQAILKLTPAPXXXXXXXXXXXXXXXXXXXXIMEGMLCTIFDI-DFFK 494
           AA G GLAF+V+  A+L+L  +                      +EG +    D      
Sbjct: 405 AASGPGLAFLVYPSAVLELPGSSIWSCLFFFMLILIGLDSQFCTVEGFITAAVDEWPRLL 464

Query: 495 RLSKPVITGIVCSFCFLVGLIFTTGAGEYWLKMFDSFAGT-IGLVVVALLEMVSVIYIYG 553
           R  K +   IVC   +L+GL   T  G Y  ++ DS+A +   L+ +   E +++ + +G
Sbjct: 465 RKRKELFIAIVCFVSYLIGLFCITEGGMYVFQLLDSYAVSGFCLLFLMFFECIAISWAFG 524

Query: 554 HERFSNDIYEMTGYRPGLYWQVTWRYVGPFI-VTCILFSSLVFMLINPPTY 603
             RF + I +M GY P  +W++ W    P I V    F+ + F+ +   TY
Sbjct: 525 VNRFYDGIRDMIGYYPCCWWKICWTITTPAICVGVFTFNIIKFVPVKYLTY 575


>AY395073-1|AAQ96729.1|  203|Apis mellifera GABA neurotransmitter
           transporter-1A protein.
          Length = 203

 Score =  122 bits (294), Expect = 9e-30
 Identities = 52/105 (49%), Positives = 75/105 (71%), Gaps = 4/105 (3%)

Query: 72  LGNIWRFPYLCQQNGGGAFLIPFLIMLVLEGVPLFLIEMAIGQKMRLGSLGVWNTIHPWL 131
           L N+WRFPYLC +NGGGAFLIP+ + LVL G+P+F +E+A+GQ + +G LGV+  I P  
Sbjct: 1   LANVWRFPYLCYKNGGGAFLIPYFLTLVLAGIPMFFMELALGQMLTVGGLGVFK-IAPLF 59

Query: 132 GGIGISSCIVTLFVALYYNVIITWVFFYLFNSIRLTADQLPWSSC 176
            GIG ++ +++ ++ +YY VI+ W  FY F S+R    +LPW SC
Sbjct: 60  KGIGYAAAVMSCWMNVYYIVILAWAIFYFFMSMR---SELPWGSC 101


  Database: bee
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 140,377
  Number of sequences in database:  429
  
Lambda     K      H
   0.327    0.142    0.456 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 173,586
Number of Sequences: 429
Number of extensions: 7100
Number of successful extensions: 38
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 21
Number of HSP's gapped (non-prelim): 10
length of query: 614
length of database: 140,377
effective HSP length: 62
effective length of query: 552
effective length of database: 113,779
effective search space: 62806008
effective search space used: 62806008
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.7 bits)
S2: 46 (22.6 bits)