Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA004147-TA|BGIBMGA004147-PA|IPR002168|Lipolytic enzyme,
IPR002018|Carboxylesterase, type B
(549 letters)
Database: bee
429 sequences; 140,377 total letters
Searching.....................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY647436-1|AAU81605.1| 567|Apis mellifera juvenile hormone este... 143 4e-36
AB083009-1|BAC54130.1| 567|Apis mellifera esterase protein. 143 4e-36
AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase ... 107 2e-25
AY526235-1|AAS20468.1| 169|Apis mellifera esterase protein. 99 5e-23
AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase ... 99 8e-23
AF213011-1|AAG43567.1| 62|Apis mellifera esterase A2 protein. 29 0.075
DQ067178-1|AAZ20250.1| 448|Apis mellifera conserved ATPase doma... 23 8.6
>AY647436-1|AAU81605.1| 567|Apis mellifera juvenile hormone
esterase protein.
Length = 567
Score = 143 bits (346), Expect = 4e-36
Identities = 129/499 (25%), Positives = 213/499 (42%), Gaps = 30/499 (6%)
Query: 11 WSLWAARLVRQPTATVRVSNGLLRGAVAHDGS-YVQYNGIPFG-SVTDETKFQAPGPEPK 68
W+L A V+ P ++ G + ++ +G Y Y GIP+ + +F+AP P
Sbjct: 17 WTLEDAPRVKTPLGAIK---GYYK--ISGNGKQYEAYEGIPYALPPVGKFRFKAPQKIPA 71
Query: 69 WDGIYDAIHEEIRCNQ--QFSTN---LTIGRRDCLTLNIYTPFNTDPGDKLVVMXXXXXX 123
W G A C Q Q N G DCL LN+Y P + P L V+
Sbjct: 72 WIGELSATKFGFPCLQYTQLPVNPRDKIEGAEDCLYLNVYVPADRTPSQSLPVIFWIHGG 131
Query: 124 XXXXXXXXXXXXXPRYLVSKGVILVTINYRLNIQGFLCLGIKEAPGNAALKDMVAALKWV 183
+YL+ VI VTINYRL I GFL + PGN LKD AL+WV
Sbjct: 132 AFQFGSGIPMGA--KYLMDSDVIFVTINYRLGILGFLSTEDEVVPGNMGLKDQSMALRWV 189
Query: 184 QRNIASFGGDPDNVTIFGESAGAASVSFLVLSPMAKGLFHKAITQSGSSLAPFGLQFKPI 243
NI FGG+P +T+ G SAG ASV + LSP++ GLF I+ SG++L + +
Sbjct: 190 SENIEWFGGNPKRITLIGLSAGGASVHYHYLSPLSAGLFQGGISISGTALNCWTQTENSL 249
Query: 244 FLASLLAKVMGFISQDPVKLYEYFMTKSDDELILTRVPRKEGHVIISEILYTPCVEKVIE 303
A + MG +++ ++ + ++ T + + P EKV
Sbjct: 250 EKAKQVGAFMGCPTRNVKEMIRCLRYRPARAIVET-LANFMRFYYNPFTPFGPVTEKVNN 308
Query: 304 GVE--PFLIQSPQEALSKGEFNKVPMIIGSNTQEGI-----MILEMDN-STMRNNIKIEK 355
PF+ ++P E ++ G+ VP + G ++EG+ I + + + N +
Sbjct: 309 DSNSLPFIDRTPIEIINSGDVQDVPWVTGVTSEEGLYPVAEFIAKPEALKLLDENWDLIA 368
Query: 356 SLPKNLNITSQEAVTEIVGNFKKIYMGDGDSFGD--LVSLSKWYGEPYLNYPSLEETELI 413
+ N T + V + Y + + + L L + + + +
Sbjct: 369 PYFLDYNYTIPKEKHVEVARLIRNYYFESNKIDETTLKHLIDVASDRFFITDGEKAARMQ 428
Query: 414 LKSSKQPIYNYLFNYSGWRNLPKLGGGSAYKDVPGATHADDLFYMFSQEWLSGFF---EN 470
K ++QP++ Y + Y G ++ ++ G++ K G HADD + + +L+ +
Sbjct: 429 AKVNRQPVWFYYYTYKGAHSISEIMSGTSNK--YGVCHADDAYMVVDTPFLASTTTTNDI 486
Query: 471 KMIERMTTLWTNFAKYGDP 489
KM + + W +F G P
Sbjct: 487 KMQKVLIDFWVSFVNNGVP 505
>AB083009-1|BAC54130.1| 567|Apis mellifera esterase protein.
Length = 567
Score = 143 bits (346), Expect = 4e-36
Identities = 129/499 (25%), Positives = 213/499 (42%), Gaps = 30/499 (6%)
Query: 11 WSLWAARLVRQPTATVRVSNGLLRGAVAHDGS-YVQYNGIPFG-SVTDETKFQAPGPEPK 68
W+L A V+ P ++ G + ++ +G Y Y GIP+ + +F+AP P
Sbjct: 17 WTLEDAPRVKTPLGAIK---GYYK--ISGNGKQYEAYEGIPYALPPVGKFRFKAPQKIPA 71
Query: 69 WDGIYDAIHEEIRCNQ--QFSTN---LTIGRRDCLTLNIYTPFNTDPGDKLVVMXXXXXX 123
W G A C Q Q N G DCL LN+Y P + P L V+
Sbjct: 72 WIGELSATKFGFPCLQYTQLPVNPRDKIEGAEDCLYLNVYVPADRTPSQSLPVIFWIHGG 131
Query: 124 XXXXXXXXXXXXXPRYLVSKGVILVTINYRLNIQGFLCLGIKEAPGNAALKDMVAALKWV 183
+YL+ VI VTINYRL I GFL + PGN LKD AL+WV
Sbjct: 132 AFQFGSGIPMGA--KYLMDSDVIFVTINYRLGILGFLSTEDEVVPGNMGLKDQSMALRWV 189
Query: 184 QRNIASFGGDPDNVTIFGESAGAASVSFLVLSPMAKGLFHKAITQSGSSLAPFGLQFKPI 243
NI FGG+P +T+ G SAG ASV + LSP++ GLF I+ SG++L + +
Sbjct: 190 SENIEWFGGNPKRITLIGLSAGGASVHYHYLSPLSAGLFQGGISISGTALNCWTQTENSL 249
Query: 244 FLASLLAKVMGFISQDPVKLYEYFMTKSDDELILTRVPRKEGHVIISEILYTPCVEKVIE 303
A + MG +++ ++ + ++ T + + P EKV
Sbjct: 250 EKAKQVGAFMGCPTRNVKEMIRCLRYRPARAIVET-LANFMRFYYNPFTPFGPVTEKVNN 308
Query: 304 GVE--PFLIQSPQEALSKGEFNKVPMIIGSNTQEGI-----MILEMDN-STMRNNIKIEK 355
PF+ ++P E ++ G+ VP + G ++EG+ I + + + N +
Sbjct: 309 DSNSLPFIDRTPIEIINSGDVQDVPWVTGVTSEEGLYPVAEFIAKPEALKLLDENWDLIA 368
Query: 356 SLPKNLNITSQEAVTEIVGNFKKIYMGDGDSFGD--LVSLSKWYGEPYLNYPSLEETELI 413
+ N T + V + Y + + + L L + + + +
Sbjct: 369 PYFLDYNYTIPKEKHVEVARLIRNYYFESNKIDETTLKHLIDVASDRFFITDGEKAARMQ 428
Query: 414 LKSSKQPIYNYLFNYSGWRNLPKLGGGSAYKDVPGATHADDLFYMFSQEWLSGFF---EN 470
K ++QP++ Y + Y G ++ ++ G++ K G HADD + + +L+ +
Sbjct: 429 AKVNRQPVWFYYYTYKGAHSISEIMSGTSNK--YGVCHADDAYMVVDTPFLASTTTTNDI 486
Query: 471 KMIERMTTLWTNFAKYGDP 489
KM + + W +F G P
Sbjct: 487 KMQKVLIDFWVSFVNNGVP 505
>AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase
protein.
Length = 628
Score = 107 bits (258), Expect = 2e-25
Identities = 105/400 (26%), Positives = 183/400 (45%), Gaps = 53/400 (13%)
Query: 142 SKGVILVTINYRLNIQGFLCLG-----IKEAPGNAALKDMVAALKWVQRNIASFGGDPDN 196
+ VI+ ++ YR+ GFL L +EAPGN L D AL+W++ N +FGGDP+
Sbjct: 187 TSNVIIASMQYRVGAFGFLYLNKHFTNSEEAPGNMGLWDQALALRWLRDNAEAFGGDPEL 246
Query: 197 VTIFGESAGAASVSFLVLSPMAKGLFHKAITQSGSSLAPFGLQF--KPIFLASLLAKVMG 254
+TIFGESAG +SVS ++SP+ +GL + I QSG+ AP+ K +A++L G
Sbjct: 247 ITIFGESAGGSSVSLHLISPVTRGLVRRGILQSGTLNAPWSYMSGEKANEVATILVDDCG 306
Query: 255 ----FISQDPVKLYEYFMTKSDDELILTRVPRKEGHVIISEILYTPCVEKVIEGVEPFLI 310
++++P ++ + + + + G IL P I+G+ FL
Sbjct: 307 CNSTMLNENPARVMACMRSVDAKTISVQQWNSYWG------ILGFPSA-PTIDGI--FLP 357
Query: 311 QSPQEALSKGEFNKVPMIIGSNTQEGIM--------ILEMDNSTMRNN---IKIEKSLPK 359
+ P + L + +F ++IG+N EG I E D ++ + I ++ K
Sbjct: 358 KHPLDLLREADFKDTEILIGNNENEGTYFILYDFNDIFEKDQASFLERERFLGIINNIFK 417
Query: 360 NLNITSQEAVTEIVGNFKKIYMGDGDSFGDLVSLSKWYGEPYLNYPSLEETELILKSSKQ 419
N++ +EA+T +++++Y +G + +V + G+ + PS+ +L +
Sbjct: 418 NMSQIEREAITFQYTDWEEVY--NGYIYQKMV--ADVVGDYFFICPSIHFAQLFADRGMK 473
Query: 420 PIYNYLFNYSGWRNLPKLGGGSAYKDVPGATHADDLFYMFSQEWLSGF----FENKMIER 475
+Y Y F NL + + G H D++ Y+F E + R
Sbjct: 474 -VYYYFFTQRTSTNL--------WGEWMGVLHGDEVEYVFGHPLNKSLKYSDKERDLSLR 524
Query: 476 MTTLWTNFAKYGDPTPEATDLLPVKWYPTNLTTPMNFVLD 515
M ++ FA G PT E ++ W + P F+ D
Sbjct: 525 MILYFSEFAYLGKPTKEDSE-----WPSYSRDEPKYFIFD 559
>AY526235-1|AAS20468.1| 169|Apis mellifera esterase protein.
Length = 169
Score = 99 bits (238), Expect = 5e-23
Identities = 49/96 (51%), Positives = 62/96 (64%)
Query: 138 RYLVSKGVILVTINYRLNIQGFLCLGIKEAPGNAALKDMVAALKWVQRNIASFGGDPDNV 197
+YL+ VI VTINYRL I GFL + PGN LKD AL+WV NI FGG+P +
Sbjct: 15 KYLMDSDVIFVTINYRLGILGFLSTEDEVVPGNMGLKDQSMALRWVSENIEWFGGNPKRI 74
Query: 198 TIFGESAGAASVSFLVLSPMAKGLFHKAITQSGSSL 233
T+ G SAG ASV + LSP++ GLF I+ SG++L
Sbjct: 75 TLIGLSAGGASVHYHYLSPLSAGLFQGGISISGTAL 110
>AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase
protein.
Length = 492
Score = 99.1 bits (236), Expect = 8e-23
Identities = 77/263 (29%), Positives = 133/263 (50%), Gaps = 31/263 (11%)
Query: 142 SKGVILVTINYRLNIQGFLCLG-----IKEAPGNAALKDMVAALKWVQRNIASFGGDPDN 196
+ VI+ ++ YR+ GFL L +EAPGN L D AL+W++ N +FGGDP+
Sbjct: 187 TSNVIIASMQYRVGAFGFLYLNKHFTNSEEAPGNMGLWDQALALRWLRDNAEAFGGDPEL 246
Query: 197 VTIFGESAGAASVSFLVLSPMAKGLFHKAITQSGSSLAPFGLQF--KPIFLASLLAKVMG 254
+TIFGESAG +SVS ++SP+ +GL + I QSG+ AP+ K +A++L G
Sbjct: 247 ITIFGESAGGSSVSLHLISPVTRGLVRRGILQSGTLNAPWSYMSGEKANEVATILVDDCG 306
Query: 255 ----FISQDPVKLYEYFMTKSDDELILTRVPRKEGHVIISEILYTPCVEKVIEGVEPFLI 310
++++P ++ + + + + G IL P I+G+ FL
Sbjct: 307 CNSTMLNENPARVMACMRSVDAKTISVQQWNSYWG------ILGFPSA-PTIDGI--FLP 357
Query: 311 QSPQEALSKGEFNKVPMIIGSNTQEGIM--------ILEMDNSTMRNN---IKIEKSLPK 359
+ P + L + +F ++IG+N EG I E D ++ + I ++ K
Sbjct: 358 KHPLDLLREADFKDTEILIGNNENEGTYFILYDFNDIFEKDQASFLERERFLGIINNIFK 417
Query: 360 NLNITSQEAVTEIVGNFKKIYMG 382
N++ +EA+T +++++Y G
Sbjct: 418 NMSQIEREAITFQYTDWEEVYNG 440
>AF213011-1|AAG43567.1| 62|Apis mellifera esterase A2 protein.
Length = 62
Score = 29.5 bits (63), Expect = 0.075
Identities = 20/63 (31%), Positives = 28/63 (44%), Gaps = 3/63 (4%)
Query: 96 DCLTLNIYTPFNTDPGDKLVVMXXXXXXXXXXXXXXXXXXXPRYLVSKGVILVTINYRLN 155
DCL L++YT N+ K VM P YL+ K V++V+ NYR+
Sbjct: 3 DCLYLDVYT--NSLDQSK-PVMFYVHEGAFISGTSSFHEMRPDYLLPKDVVVVSSNYRVG 59
Query: 156 IQG 158
G
Sbjct: 60 AFG 62
>DQ067178-1|AAZ20250.1| 448|Apis mellifera conserved ATPase domain
protein protein.
Length = 448
Score = 22.6 bits (46), Expect = 8.6
Identities = 9/18 (50%), Positives = 11/18 (61%)
Query: 220 GLFHKAITQSGSSLAPFG 237
G FH+ TQ GSS+ G
Sbjct: 190 GKFHRVCTQIGSSMKSVG 207
Database: bee
Posted date: Oct 5, 2007 11:13 AM
Number of letters in database: 140,377
Number of sequences in database: 429
Lambda K H
0.319 0.137 0.419
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 164,313
Number of Sequences: 429
Number of extensions: 7301
Number of successful extensions: 23
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 8
Number of HSP's gapped (non-prelim): 13
length of query: 549
length of database: 140,377
effective HSP length: 61
effective length of query: 488
effective length of database: 114,208
effective search space: 55733504
effective search space used: 55733504
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 46 (22.6 bits)
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