Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA003395-TA|BGIBMGA003395-PA|IPR000795|Protein synthesis
factor, GTP-binding, IPR004161|Elongation factor Tu, domain 2,
IPR004160|Elongation factor Tu, C-terminal,
IPR009001|EF-Tu/eEF-1alpha/eIF2-gamma, C-terminal
(671 letters)
Database: bee
429 sequences; 140,377 total letters
Searching.....................................................done
Score E
Sequences producing significant alignments: (bits) Value
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 310 2e-86
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 305 7e-85
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 206 5e-55
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 138 1e-34
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 48 2e-07
EF013227-1|ABK54581.1| 119|Apis mellifera elongation factor 1-a... 39 1e-04
AB047034-1|BAB64310.1| 1598|Apis mellifera mblk-1 protein. 28 0.29
AY500239-1|AAR92109.1| 555|Apis mellifera neuronal nicotinic ac... 24 3.5
AF393493-1|AAL60418.1| 142|Apis mellifera odorant binding prote... 24 3.5
AF166497-1|AAD51945.1| 142|Apis mellifera putative odorant-bind... 24 3.5
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 23 8.1
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 310 bits (762), Expect = 2e-86
Identities = 162/443 (36%), Positives = 254/443 (57%), Gaps = 18/443 (4%)
Query: 241 GSDKDHLYIVVIGHVDAGKSTLMGRLLCELGEVSQKTLHKYEQESKKIGKQSFMYAWVLD 300
G +K H+ IVVIGHVD+GKST G L+ + G + ++T+ K+E+E++++GK SF YAWVLD
Sbjct: 2 GKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLD 61
Query: 301 ETGEERVRGITMDIGRAQIETKTKRVIILDAPGHADFIPNMITGAGQADVAMLVVDATRG 360
+ ER RGIT+DI + ET V I+DAPGH DFI NMITG QAD A+L+V A G
Sbjct: 62 KLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGIG 121
Query: 361 EFESGFDFGGQTREHALLVRSLGVSQLAVAINKLDTAN--WSQDRFNEITNKLKVFLKQA 418
EFE+G GQTREHALL +LGV QL V +NK+D + +S+ RF EI ++ ++K+
Sbjct: 122 EFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKKI 181
Query: 419 GFKDTDVTYVPCSGLIGENLVKPASEPELTKWYK------------GPCLLDVIDKFNVP 466
G+ V +VP SG G+N+++P+ + T WYK G L++ +D P
Sbjct: 182 GYNTASVAFVPISGWHGDNMLEPSPK---TPWYKGWKVERKDGNADGKTLIEALDAILPP 238
Query: 467 ERPVSKPLRISINDVFKGTGSGFCVSGRIENGVLKKGDKVLVCPTKEMAEVRSLSINDLT 526
RP K LR+ + DV+K G G GR+E G+LK G V P EV+S+ ++
Sbjct: 239 SRPTDKALRLPLQDVYKIGGIGTVPVGRVETGILKPGMLVTFAPAALTTEVKSVEMHHEA 298
Query: 527 NNVAFAGDQVTLTLSGVDMQNVSVGYILSDPIEQ-VSVTTRFEARIVVFNVKVPITKGFP 585
A GD V + + ++ + GY+ D Q F A+++V N I+ G+
Sbjct: 299 LTEALPGDNVGFNVKNISVKELRRGYVAGDSKNQPPRGAADFTAQVIVLNHPGQISNGYT 358
Query: 586 VLVHYQSLVESANIVKLKALLSKSTGELIKKKPRCLGNNSVAVVEIETSRPICIERYKDV 645
++ + + ++K + TG+ ++ P+ + + A+V ++ ++P+C+E +++
Sbjct: 359 PVLDCHTAHIACKFAEIKEKCDRRTGKTTEENPKSIKSGDAAIVMLQPTKPMCVEAFQEF 418
Query: 646 KELGRVMLRVAGVTIAAGLVTDI 668
LGR +R T+A G++ +
Sbjct: 419 PPLGRFAVRDMRQTVAVGVIKSV 441
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 305 bits (749), Expect = 7e-85
Identities = 162/437 (37%), Positives = 248/437 (56%), Gaps = 12/437 (2%)
Query: 241 GSDKDHLYIVVIGHVDAGKSTLMGRLLCELGEVSQKTLHKYEQESKKIGKQSFMYAWVLD 300
G +K H+ IVVIGHVD+GKST G L+ + G + ++T+ K+E+E++++GK SF YAWVLD
Sbjct: 2 GKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVLD 61
Query: 301 ETGEERVRGITMDIGRAQIETKTKRVIILDAPGHADFIPNMITGAGQADVAMLVVDATRG 360
+ ER RGIT+DI + ET V I+DAPGH DFI NMITG QAD A+L+V A G
Sbjct: 62 KLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 121
Query: 361 EFESGFDFGGQTREHALLVRSLGVSQLAVAINKLDTAN--WSQDRFNEITNKLKVFLKQA 418
EFE+G GQTREHALL +LGV QL V +NK+D+ +S+ RF EI ++ ++K+
Sbjct: 122 EFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKI 181
Query: 419 GFKDTDVTYVPCSGLIGENLVKPASE-PELTKW--------YKGPCLLDVIDKFNVPERP 469
G+ V +VP SG G+N+++ +S+ P W +G CL++ +D P RP
Sbjct: 182 GYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILPPTRP 241
Query: 470 VSKPLRISINDVFKGTGSGFCVSGRIENGVLKKGDKVLVCPTKEMAEVRSLSINDLTNNV 529
K LR+ + DV+K G G GR+E GVLK G V P EV+S+ ++
Sbjct: 242 TDKALRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPAGLTTEVKSVEMHHEALQE 301
Query: 530 AFAGDQVTLTLSGVDMQNVSVGYILSDPIEQ-VSVTTRFEARIVVFNVKVPITKGFPVLV 588
A GD V + V ++ + GY+ D F A+++V N I+ G+ ++
Sbjct: 302 AVPGDNVGFNVKNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVL 361
Query: 589 HYQSLVESANIVKLKALLSKSTGELIKKKPRCLGNNSVAVVEIETSRPICIERYKDVKEL 648
+ + +K + G+ ++ P+ + + A+V + S+P+C E +++ L
Sbjct: 362 DCHTAHIACKFADIKEKCDRRNGKTTEENPKSIKSGDAAIVMLVPSKPMCAEAFQEFPPL 421
Query: 649 GRVMLRVAGVTIAAGLV 665
GR +R T+A G++
Sbjct: 422 GRFAVRDMRQTVAVGVI 438
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 206 bits (503), Expect = 5e-55
Identities = 112/271 (41%), Positives = 155/271 (57%), Gaps = 11/271 (4%)
Query: 297 WVLDETGEERVRGITMDIGRAQIETKTKRVIILDAPGHADFIPNMITGAGQADVAMLVVD 356
WVLD+ ER RGIT+DI + ET V I+DAPGH DFI NMITG QAD A+L+V
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 357 ATRGEFESGFDFGGQTREHALLVRSLGVSQLAVAINKLDTAN--WSQDRFNEITNKLKVF 414
A GEFE+G GQTREHALL +LGV QL V +NK+D+ +S+ RF EI ++ +
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120
Query: 415 LKQAGFKDTDVTYVPCSGLIGENLVKPASE-PELTKW--------YKGPCLLDVIDKFNV 465
+K+ G+ V +VP SG G+N+++ +S+ P W +G CL++ +D
Sbjct: 121 IKKIGYNPAAVAFVPISGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILP 180
Query: 466 PERPVSKPLRISINDVFKGTGSGFCVSGRIENGVLKKGDKVLVCPTKEMAEVRSLSINDL 525
P RP K LR+ + DV+K G G GR+E GVLK G V P EV+S+ ++
Sbjct: 181 PTRPTDKALRLPLQDVYKIGGIGTVPVGRVETGVLKPGMVVTFAPAGLTTEVKSVEMHHE 240
Query: 526 TNNVAFAGDQVTLTLSGVDMQNVSVGYILSD 556
A GD V + V ++ + GY+ D
Sbjct: 241 ALQEAVPGDNVGFNVKNVSVKELRRGYVAGD 271
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 138 bits (335), Expect = 1e-34
Identities = 74/171 (43%), Positives = 102/171 (59%), Gaps = 11/171 (6%)
Query: 313 DIGRAQIETKTKRVIILDAPGHADFIPNMITGAGQADVAMLVVDATRGEFESGFDFGGQT 372
DI + ET V I+DAPGH DFI NMITG QAD A+L+V A GEFE+G GQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 373 REHALLVRSLGVSQLAVAINKLDTAN--WSQDRFNEITNKLKVFLKQAGFKDTDVTYVPC 430
REHALL +LGV QL V +NK+D+ +S+ RF EI ++ ++K+ G+ V +VP
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 431 SGLIGENLVKPASE-PELTKW--------YKGPCLLDVIDKFNVPERPVSK 472
SG G+N+++ +S+ P W +G CL++ +D P RP K
Sbjct: 121 SGWHGDNMLEVSSKMPWFKGWTVERKEGKVEGKCLIEALDAILPPTRPTDK 171
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 48.4 bits (110), Expect = 2e-07
Identities = 85/324 (26%), Positives = 134/324 (41%), Gaps = 33/324 (10%)
Query: 289 GKQSFMYAWVLDETGEERVRGITMDIGRAQIETKT-KRVIILDAPGHADFIPNMITGAGQ 347
GK + + A + GIT IG + ++ +RV LD PGHA FI GA
Sbjct: 157 GKTTLLDALRNTSIAKSEFGGITQCIGAFDVTLESGERVTFLDTPGHAAFISMRHRGAHI 216
Query: 348 ADVAMLVVDATRGEFESGFDFGGQTREHALLVRSLGVSQLAVAINKLDTANWSQDRFNEI 407
D+ +LVV A G E QT + + + V + VAINK+D N +
Sbjct: 217 TDIVVLVVAADDGVKE-------QTLQSIEMAKDAKV-PIIVAINKIDKPNIDIIKVQYE 268
Query: 408 TNKLKVFLKQAGFKDTDVTYVPCSGLIGENLVKPASEPELTKWYKGPCLLDVIDKFNVPE 467
K + +++ G ++ V S L G NL + +E + + +++D E
Sbjct: 269 LAKHGIVIEELG---GEIQCVKISALKGINL-RELTEAIIVQ-------AELMDLKGDLE 317
Query: 468 RPVSKPLRISINDVFKGTGSGFCVSGRIENGVLKKGDKVLVCPTKEMAEVRSLSINDLTN 527
V I D G G V+ I+ G LKKG L+ A+VRS+ ND N
Sbjct: 318 GLVEG----VIIDCSNHIGRGKLVTALIQRGTLKKG--CLLVSGIASAKVRSM-FNDSGN 370
Query: 528 NVAFA-GDQVTLTLSGVDMQNV--SVGYILSDPIEQVSVTTRFEARIVVFNVKVPITKGF 584
+ A +V L ++ NV + + +D I Q + R + R + +
Sbjct: 371 PILKAKPSEVVQILGWKELPNVGDEILEVENDKILQEVIKFRQKQRAEILAKEHKAAADQ 430
Query: 585 PVLVHYQSLVESANIVKLKALLSK 608
+ H L+E ++K+K L K
Sbjct: 431 KLEKH---LIEYKKMLKIKRLFGK 451
Score = 37.1 bits (82), Expect = 5e-04
Identities = 18/43 (41%), Positives = 24/43 (55%)
Query: 224 KVKENKIDAKKQYANERGSDKDHLYIVVIGHVDAGKSTLMGRL 266
K ENK K+ NE K H + ++GHVD GK+TL+ L
Sbjct: 123 KTMENKDITKRPLPNESQLIKRHPIVTIMGHVDHGKTTLLDAL 165
>EF013227-1|ABK54581.1| 119|Apis mellifera elongation factor
1-alpha protein.
Length = 119
Score = 39.1 bits (87), Expect = 1e-04
Identities = 20/107 (18%), Positives = 49/107 (45%), Gaps = 1/107 (0%)
Query: 530 AFAGDQVTLTLSGVDMQNVSVGYILSDPIEQVSV-TTRFEARIVVFNVKVPITKGFPVLV 588
A GD V + + ++ + GY+ D Q F A+++V N I+ G+ ++
Sbjct: 13 ALPGDNVGFNVKNISVKELRRGYVAGDSKNQPPRGAADFTAQVIVLNHPGQISNGYTPVL 72
Query: 589 HYQSLVESANIVKLKALLSKSTGELIKKKPRCLGNNSVAVVEIETSR 635
+ + ++K + TG+ ++ P+ + + A+V ++ ++
Sbjct: 73 DCHTAHIACKFAEIKEKCDRRTGKTTEENPKSIKSGDAAIVMLQPTK 119
>AB047034-1|BAB64310.1| 1598|Apis mellifera mblk-1 protein.
Length = 1598
Score = 27.9 bits (59), Expect = 0.29
Identities = 10/37 (27%), Positives = 19/37 (51%)
Query: 191 QEPKPNSRPQTPRGQSPHPEKSKTEQNDDMKNLKVKE 227
Q+ +P + Q P+ Q P P++ + +Q + KE
Sbjct: 1513 QQQQPQQQSQQPQQQQPQPQQQQQQQQQQQPQQQQKE 1549
Score = 24.2 bits (50), Expect = 3.5
Identities = 11/54 (20%), Positives = 24/54 (44%)
Query: 192 EPKPNSRPQTPRGQSPHPEKSKTEQNDDMKNLKVKENKIDAKKQYANERGSDKD 245
+ + N + RGQS ++ K +++ D ++ K ++Y +D D
Sbjct: 282 DDEENEEEEDGRGQSEAEKRLKLDEDADGAVSPLRREKDRGSREYPTSNATDTD 335
Score = 23.8 bits (49), Expect = 4.6
Identities = 12/68 (17%), Positives = 28/68 (41%)
Query: 175 TSVDQKSAVIKGFKVDQEPKPNSRPQTPRGQSPHPEKSKTEQNDDMKNLKVKENKIDAKK 234
++ D K V + Q+ +P + Q + Q P + + +Q + ++ + +
Sbjct: 1485 SACDSKLIVDHSSQKTQQQQPQQQQQQQQQQQPQQQSQQPQQQQPQPQQQQQQQQQQQPQ 1544
Query: 235 QYANERGS 242
Q E G+
Sbjct: 1545 QQQKEYGA 1552
>AY500239-1|AAR92109.1| 555|Apis mellifera neuronal nicotinic
acetylcholine receptoralpha7-1 protein.
Length = 555
Score = 24.2 bits (50), Expect = 3.5
Identities = 13/25 (52%), Positives = 18/25 (72%), Gaps = 3/25 (12%)
Query: 456 LLDVIDKFNVPERPV---SKPLRIS 477
L D++D +NV ERPV S+PL +S
Sbjct: 28 LNDLLDTYNVLERPVGNESEPLVLS 52
>AF393493-1|AAL60418.1| 142|Apis mellifera odorant binding protein
ASP2 protein.
Length = 142
Score = 24.2 bits (50), Expect = 3.5
Identities = 10/27 (37%), Positives = 15/27 (55%)
Query: 159 NTVKTTTALVAPKVIATSVDQKSAVIK 185
NT+ T T L+A + +DQ + V K
Sbjct: 2 NTLVTVTCLLAALTVVRGIDQDTVVAK 28
>AF166497-1|AAD51945.1| 142|Apis mellifera putative odorant-binding
protein ASP2 protein.
Length = 142
Score = 24.2 bits (50), Expect = 3.5
Identities = 10/27 (37%), Positives = 15/27 (55%)
Query: 159 NTVKTTTALVAPKVIATSVDQKSAVIK 185
NT+ T T L+A + +DQ + V K
Sbjct: 2 NTLVTVTCLLAALTVVRGIDQDTVVAK 28
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 23.0 bits (47), Expect = 8.1
Identities = 15/38 (39%), Positives = 21/38 (55%), Gaps = 2/38 (5%)
Query: 228 NKIDAKKQYANERG--SDKDHLYIVVIGHVDAGKSTLM 263
+K+D K A R S + + I IGHV GKST++
Sbjct: 22 SKLDVSKLTALSREVISRQATINIGTIGHVAHGKSTIV 59
Database: bee
Posted date: Oct 5, 2007 11:13 AM
Number of letters in database: 140,377
Number of sequences in database: 429
Lambda K H
0.316 0.133 0.379
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 171,994
Number of Sequences: 429
Number of extensions: 6940
Number of successful extensions: 40
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 16
Number of HSP's gapped (non-prelim): 15
length of query: 671
length of database: 140,377
effective HSP length: 62
effective length of query: 609
effective length of database: 113,779
effective search space: 69291411
effective search space used: 69291411
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 47 (23.0 bits)
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