Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA002894-TA|BGIBMGA002894-PA|IPR002048|Calcium-binding
EF-hand, IPR000795|Protein synthesis factor, GTP-binding,
IPR004161|Elongation factor Tu, domain 2, IPR004160|Elongation factor
Tu, C-terminal, IPR009001|EF-Tu/eEF-1alpha/eIF2-gamma, C-terminal,
IPR009000|Translation factor
(635 letters)
Database: bee
429 sequences; 140,377 total letters
Searching.....................................................done
Score E
Sequences producing significant alignments: (bits) Value
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 64 4e-12
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 63 6e-12
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 42 1e-05
EF013227-1|ABK54581.1| 119|Apis mellifera elongation factor 1-a... 42 2e-05
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 42 2e-05
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 38 3e-04
AY686596-1|AAT96374.1| 1946|Apis mellifera Dscam protein. 24 4.4
AY268031-1|AAP23056.1| 810|Apis mellifera dorsal protein splice... 24 4.4
DQ485319-1|ABF21078.1| 175|Apis mellifera icarapin variant 2 pr... 23 5.8
DQ485318-1|ABF21077.1| 223|Apis mellifera icarapin variant 1 pr... 23 5.8
DQ435335-1|ABD92650.1| 135|Apis mellifera OBP18 protein. 23 5.8
AY939856-1|AAX33236.1| 223|Apis mellifera venom carbohydrate-ri... 23 5.8
AY897570-1|AAW81036.1| 223|Apis mellifera venom protein 2 protein. 23 5.8
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 63.7 bits (148), Expect = 4e-12
Identities = 74/311 (23%), Positives = 119/311 (38%), Gaps = 24/311 (7%)
Query: 224 EKSSKVITFIDLAGHERYLKTTVFGMTGHAPDFGMLMIGAN-----AGIV--GMTKEHLG 276
E + +T ID GH ++K + G T A D +L++ A AGI G T+EH
Sbjct: 81 ETAKYYVTIIDAPGHRDFIKNMITG-TSQA-DCAVLIVAAGIGEFEAGISKNGQTREHAL 138
Query: 277 LALALSVPVFVV-VTKIDMCPPNVLQDNLKLLIRILKSSGCRKVPVMVKSKDDVIVSA-- 333
LA L V +V V K+DM P + + + + + SS +K+ S V +S
Sbjct: 139 LAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEV-SSYIKKIGYNTASVAFVPISGWH 197
Query: 334 -TNFVSQR-LCPIFQ---VSNVSGXXXXXXXXXXXXXKTRMPSHEDKPAEFQIDDTYXXX 388
N + P ++ V G DK + D Y
Sbjct: 198 GDNMLEPSPKTPWYKGWKVERKDGNADGKTLIEALDAILPPSRPTDKALRLPLQDVYKIG 257
Query: 389 XXXXXXXXXALAGVVRAGDTLALGPDALGRFHPVAVKSIHRKRMPVHEVRGGQTASFALK 448
G+++ G + P AL VKS+ + E G F +K
Sbjct: 258 GIGTVPVGRVETGILKPGMLVTFAPAAL----TTEVKSVEMHHEALTEALPGDNVGFNVK 313
Query: 449 KVKRSQIRKGMVM-VSPSLNPQACWQFEGEILVLHHPTTISSRYQAMVHCGSIRQTASIL 507
+ ++R+G V S + P+ F +++VL+HP IS+ Y ++ C +
Sbjct: 314 NISVKELRRGYVAGDSKNQPPRGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKFA 373
Query: 508 SMSRDC-LRTG 517
+ C RTG
Sbjct: 374 EIKEKCDRRTG 384
Score = 27.9 bits (59), Expect = 0.27
Identities = 11/24 (45%), Positives = 17/24 (70%)
Query: 126 MEIRVAVVGNVDAGKSTLLGVLTH 149
+ I + V+G+VD+GKST G L +
Sbjct: 6 IHINIVVIGHVDSGKSTTTGHLIY 29
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 63.3 bits (147), Expect = 6e-12
Identities = 75/313 (23%), Positives = 118/313 (37%), Gaps = 25/313 (7%)
Query: 224 EKSSKVITFIDLAGHERYLKTTVFGMTGHAPDFGMLMIGAN-----AGIV--GMTKEHLG 276
E S +T ID GH ++K + G T A D +L++ A AGI G T+EH
Sbjct: 81 ETSKYYVTIIDAPGHRDFIKNMITG-TSQA-DCAVLIVAAGTGEFEAGISKNGQTREHAL 138
Query: 277 LALALSVPVFVV-VTKIDMCPPNVLQDNLKLLIRILKSSGCRKVPVMVKSKDDVIVSATN 335
LA L V +V V K+D P + + + + + SS +K+ + V +S +
Sbjct: 139 LAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEV-SSYIKKIGYNPAAVAFVPISGWH 197
Query: 336 F-----VSQRLCPIFQ---VSNVSGXXXXXXXXXXXXXKTRMPSHEDKPAEFQIDDTYXX 387
VS ++ P F+ V G DK + D Y
Sbjct: 198 GDNMLEVSSKM-PWFKGWTVERKEGKVEGKCLIEALDAILPPTRPTDKALRLPLQDVYKI 256
Query: 388 XXXXXXXXXXALAGVVRAGDTLALGPDALGRFHPVAVKSIHRKRMPVHEVRGGQTASFAL 447
GV++ G + P L VKS+ + E G F +
Sbjct: 257 GGIGTVPVGRVETGVLKPGMVVTFAPAGL----TTEVKSVEMHHEALQEAVPGDNVGFNV 312
Query: 448 KKVKRSQIRKGMVMVSPSLN-PQACWQFEGEILVLHHPTTISSRYQAMVHCGSIRQTASI 506
K V ++R+G V N P+ F +++VL+HP IS+ Y ++ C +
Sbjct: 313 KNVSVKELRRGYVAGDSKNNPPKGAADFTAQVIVLNHPGQISNGYTPVLDCHTAHIACKF 372
Query: 507 LSMSRDCLRTGDK 519
+ C R K
Sbjct: 373 ADIKEKCDRRNGK 385
Score = 27.9 bits (59), Expect = 0.27
Identities = 11/24 (45%), Positives = 17/24 (70%)
Query: 126 MEIRVAVVGNVDAGKSTLLGVLTH 149
+ I + V+G+VD+GKST G L +
Sbjct: 6 IHINIVVIGHVDSGKSTTTGHLIY 29
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 42.3 bits (95), Expect = 1e-05
Identities = 25/88 (28%), Positives = 45/88 (51%), Gaps = 6/88 (6%)
Query: 225 KSSKVITFIDLAGHERYLKTTVFGMTGHAPDFGMLMIGANAGIVGMTKEHLGLALALSVP 284
+S + +TF+D GH ++ G H D +L++ A+ G+ T + + +A VP
Sbjct: 190 ESGERVTFLDTPGHAAFISMRHRG--AHITDIVVLVVAADDGVKEQTLQSIEMAKDAKVP 247
Query: 285 VFVVVTKIDMCPPNVLQDNLKLLIRILK 312
+ V + KID PN+ D +K+ + K
Sbjct: 248 IIVAINKID--KPNI--DIIKVQYELAK 271
Score = 24.6 bits (51), Expect = 2.5
Identities = 10/18 (55%), Positives = 14/18 (77%)
Query: 130 VAVVGNVDAGKSTLLGVL 147
V ++G+VD GK+TLL L
Sbjct: 148 VTIMGHVDHGKTTLLDAL 165
>EF013227-1|ABK54581.1| 119|Apis mellifera elongation factor
1-alpha protein.
Length = 119
Score = 41.9 bits (94), Expect = 2e-05
Identities = 24/95 (25%), Positives = 42/95 (44%), Gaps = 2/95 (2%)
Query: 425 KSIHRKRMPVHEVRGGQTASFALKKVKRSQIRKGMVM-VSPSLNPQACWQFEGEILVLHH 483
KS+ + E G F +K + ++R+G V S + P+ F +++VL+H
Sbjct: 1 KSVEMHHEALTEALPGDNVGFNVKNISVKELRRGYVAGDSKNQPPRGAADFTAQVIVLNH 60
Query: 484 PTTISSRYQAMVHCGSIRQTASILSMSRDC-LRTG 517
P IS+ Y ++ C + + C RTG
Sbjct: 61 PGQISNGYTPVLDCHTAHIACKFAEIKEKCDRRTG 95
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 41.5 bits (93), Expect = 2e-05
Identities = 61/253 (24%), Positives = 94/253 (37%), Gaps = 24/253 (9%)
Query: 224 EKSSKVITFIDLAGHERYLKTTVFGMTGHAPDFGMLMIGAN-----AGIV--GMTKEHLG 276
E S +T ID GH ++K + G T A D +L++ A AGI G T+EH
Sbjct: 24 ETSKYYVTIIDAPGHRDFIKNMITG-TSQA-DCAVLIVAAGTGEFEAGISKNGQTREHAL 81
Query: 277 LALALSVPVFVV-VTKIDMCPPNVLQDNLKLLIRILKSSGCRKVPVMVKSKDDVIVSATN 335
LA L V +V V K+D P + + + + + SS +K+ + V +S +
Sbjct: 82 LAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEV-SSYIKKIGYNPAAVAFVPISGWH 140
Query: 336 -----FVSQRLCPIFQ---VSNVSGXXXXXXXXXXXXXKTRMPSHEDKPAEFQIDDTYXX 387
VS ++ P F+ V G DK + D Y
Sbjct: 141 GDNMLEVSSKM-PWFKGWTVERKEGKVEGKCLIEALDAILPPTRPTDKALRLPLQDVYKI 199
Query: 388 XXXXXXXXXXALAGVVRAGDTLALGPDALGRFHPVAVKSIHRKRMPVHEVRGGQTASFAL 447
GV++ G + P L VKS+ + E G F +
Sbjct: 200 GGIGTVPVGRVETGVLKPGMVVTFAPAGL----TTEVKSVEMHHEALQEAVPGDNVGFNV 255
Query: 448 KKVKRSQIRKGMV 460
K V ++R+G V
Sbjct: 256 KNVSVKELRRGYV 268
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 37.5 bits (83), Expect = 3e-04
Identities = 30/82 (36%), Positives = 40/82 (48%), Gaps = 10/82 (12%)
Query: 224 EKSSKVITFIDLAGHERYLKTTVFGMTGHAPDFGMLMIGAN-----AGIV--GMTKEHLG 276
E S +T ID GH ++K + G T A D +L++ A AGI G T+EH
Sbjct: 8 ETSKYYVTIIDAPGHRDFIKNMITG-TSQA-DCAVLIVAAGTGEFEAGISKNGQTREHAL 65
Query: 277 LALALSVPVFVV-VTKIDMCPP 297
LA L V +V V K+D P
Sbjct: 66 LAFTLGVKQLIVGVNKMDSTEP 87
>AY686596-1|AAT96374.1| 1946|Apis mellifera Dscam protein.
Length = 1946
Score = 23.8 bits (49), Expect = 4.4
Identities = 11/35 (31%), Positives = 16/35 (45%), Gaps = 4/35 (11%)
Query: 555 PEPMSLKPARHDRRNPQHNNHAADESDSQHVQESD 589
PEP P HD+ N N D +S + E++
Sbjct: 1857 PEPPPPPPRNHDQNNSSFN----DSKESNEISEAE 1887
>AY268031-1|AAP23056.1| 810|Apis mellifera dorsal protein splice
variant B protein.
Length = 810
Score = 23.8 bits (49), Expect = 4.4
Identities = 12/36 (33%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
Query: 110 LTGQYLLRTELDN-NDFMEIRVAVVGNVDAGKSTLL 144
L Y ++ LD+ N MEI + N+D K+T++
Sbjct: 394 LNDLYNVKNTLDSYNGSMEINQNIAQNIDHAKNTII 429
>DQ485319-1|ABF21078.1| 175|Apis mellifera icarapin variant 2
precursor protein.
Length = 175
Score = 23.4 bits (48), Expect = 5.8
Identities = 13/49 (26%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
Query: 212 PDHGTLDWVKICEKSSKVITFIDLAGHERYLKTTVFGMTGHAPDFGMLM 260
P+ G ++W KI E ++ T + GH + T + T + D+ L+
Sbjct: 63 PEQGVVNWNKIPEGANTTSTTKIIDGHVVTINETTY--TDGSDDYSTLI 109
>DQ485318-1|ABF21077.1| 223|Apis mellifera icarapin variant 1
precursor protein.
Length = 223
Score = 23.4 bits (48), Expect = 5.8
Identities = 13/49 (26%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
Query: 212 PDHGTLDWVKICEKSSKVITFIDLAGHERYLKTTVFGMTGHAPDFGMLM 260
P+ G ++W KI E ++ T + GH + T + T + D+ L+
Sbjct: 111 PEQGVVNWNKIPEGANTTSTTKIIDGHVVTINETTY--TDGSDDYSTLI 157
>DQ435335-1|ABD92650.1| 135|Apis mellifera OBP18 protein.
Length = 135
Score = 23.4 bits (48), Expect = 5.8
Identities = 15/52 (28%), Positives = 25/52 (48%), Gaps = 2/52 (3%)
Query: 184 GFDNMGNALDAVINSTCTNILEGNVVNK--PDHGTLDWVKICEKSSKVITFI 233
G+D+ GN + VI L+ N VNK + + + KS+K++ I
Sbjct: 72 GYDDGGNFNEVVIREIAEIFLDENGVNKLITECSAISDADLAVKSAKLLKCI 123
>AY939856-1|AAX33236.1| 223|Apis mellifera venom carbohydrate-rich
protein precursor protein.
Length = 223
Score = 23.4 bits (48), Expect = 5.8
Identities = 13/49 (26%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
Query: 212 PDHGTLDWVKICEKSSKVITFIDLAGHERYLKTTVFGMTGHAPDFGMLM 260
P+ G ++W KI E ++ T + GH + T + T + D+ L+
Sbjct: 111 PEQGVVNWNKIPEGANTTSTTKIIDGHVVTINETTY--TDGSDDYSTLI 157
>AY897570-1|AAW81036.1| 223|Apis mellifera venom protein 2 protein.
Length = 223
Score = 23.4 bits (48), Expect = 5.8
Identities = 13/49 (26%), Positives = 24/49 (48%), Gaps = 2/49 (4%)
Query: 212 PDHGTLDWVKICEKSSKVITFIDLAGHERYLKTTVFGMTGHAPDFGMLM 260
P+ G ++W KI E ++ T + GH + T + T + D+ L+
Sbjct: 111 PEQGVVNWNKIPEGANTTSTTKIIDGHVVAINETTY--TDGSDDYSTLI 157
Database: bee
Posted date: Oct 5, 2007 11:13 AM
Number of letters in database: 140,377
Number of sequences in database: 429
Lambda K H
0.318 0.134 0.392
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 160,269
Number of Sequences: 429
Number of extensions: 6074
Number of successful extensions: 31
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 8
Number of HSP's that attempted gapping in prelim test: 20
Number of HSP's gapped (non-prelim): 19
length of query: 635
length of database: 140,377
effective HSP length: 62
effective length of query: 573
effective length of database: 113,779
effective search space: 65195367
effective search space used: 65195367
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 47 (23.0 bits)
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