GeneModel BLAST Result against fruitfly

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA002845-TA|BGIBMGA002845-PA|IPR009543|Vacuolar protein
sorting-associated protein
         (1354 letters)

Database: fruitfly 
           52,641 sequences; 24,830,863 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AE014296-2182|AAF49887.2| 3892|Drosophila melanogaster CG32113-P...   180   9e-45
AE013599-453|AAF59228.1| 3242|Drosophila melanogaster CG2093-PA ...    44   0.001
AE014297-4492|AAF56978.3| 3731|Drosophila melanogaster CG15523-P...    43   0.003

>AE014296-2182|AAF49887.2| 3892|Drosophila melanogaster CG32113-PA
            protein.
          Length = 3892

 Score =  180 bits (439), Expect = 9e-45
 Identities = 97/225 (43%), Positives = 136/225 (60%), Gaps = 5/225 (2%)

Query: 1028 QIRLSMCTANXXXXXXXXXXXXXXXXXIKFEDAAVELEPF-ERVHVFETASYLGRQMLAH 1086
            Q+RLS  T++                 IKFEDA +EL+ + +++H FET     +++  H
Sbjct: 3504 QVRLSAFTSSKLPVELHETKKALGLTLIKFEDALIELDRYSDKLH-FETMDVYRKELKKH 3562

Query: 1087 FKDELKWQAAKILGSVDFLGNPLGFVADVSEGVTGLLLEGNVGALLKNVTHGISNSAAKV 1146
            + +++KW AA ILGSVDFLGNPLGF  D+SEGV+GL+ EG+V +L+KNVTHGISNS AK+
Sbjct: 3563 YINQVKWHAAAILGSVDFLGNPLGFANDLSEGVSGLIFEGSVKSLVKNVTHGISNSTAKL 3622

Query: 1147 SESLGDGLERVVWDEAHEETRRRI---RSXXXXXXXXXXXXXXXXXXXXXMTSLVKHSYE 1203
            +E+L D L +VV D+   ETR+RI   +S                     +TS+V+H+Y+
Sbjct: 3623 TETLSDSLGKVVLDDHDNETRQRILELQSNTSGGHLAAGLKGLGFGLLGGVTSIVRHTYD 3682

Query: 1204 GATQEXXXXXXXXXXXXXXXXXXXPVIGVLDLAAETASALRDTSR 1248
            GAT +                   P+IGVLDLA+ETASA+R+TSR
Sbjct: 3683 GATSDGVPGFLSGLGKGLVGTVTKPIIGVLDLASETASAVRETSR 3727



 Score =  151 bits (366), Expect = 7e-36
 Identities = 115/405 (28%), Positives = 175/405 (43%), Gaps = 28/405 (6%)

Query: 409  EARIVFEVSLEGSARKLVTVRSALQLVNKLPHPVEVRMDHAPSTAQWVGGGVRSARV-SP 467
            ++RIVF++SL GSA+KL+ V+S+L ++NKL   V ++M    + +     G+ S  V  P
Sbjct: 2572 KSRIVFDISLIGSAQKLIKVKSSLGVINKLDRNVFLKMTLKGTHSD----GLSSISVIKP 2627

Query: 468  GDSWAAPLEAEGGALWXXXXXXXXXXXXXXXXXX--IHWKDASPSKPL-LHYSCTAPPDH 524
             D  + PL+    +L+                    I WK         L          
Sbjct: 2628 NDELSVPLKFIDASLYVAHNTSESDAYEDTGFSNEEILWKACGKDDTRQLLAGYDTNKSI 2687

Query: 525  VYRFCCVMIRERFPPDRGPPIAGHTLTXXXXXXXXXXXXXXXQYRSVNKAGLVVHGSVAP 584
            +Y F  V I       +   + GH +T                ++    A     G +  
Sbjct: 2688 LYTF--VNISREIYHCKEQNLPGHKITLLPPLKINNMLCCDLMFKIHEHAT----GRINS 2741

Query: 585  GDTKPFHQVNVEEGVELSIKLEGFGWXXXXXXXXXXXXXXXXXXXXXFSAKIKLRDTRSR 644
             ++   + VN+ + + LSI L+ F                          K+KL D + R
Sbjct: 2742 SESVNIYNVNICQPLNLSITLDNF--------QLSGQLKIPVSHRGVIEPKLKLIDIQKR 2793

Query: 645  RLYLNARVTVKKTDGMKVSVSAAYWLVNRTGLPLVYRAEGSAADAAGQFAEHELARVVAP 704
             L++   +      GM++ +SA  W++N+TGLPL+Y+ EG++  AAGQF EHE AR VAP
Sbjct: 2794 ELHVRVSIQSVPGKGMELYISAPVWIINKTGLPLIYKQEGTSHTAAGQFEEHETARQVAP 2853

Query: 705  LLFSFAEPDARPTLCARLGRGLPGNPEWCSPFGLGPGVSVKRLXXXXXXXXXXXXXXXXX 764
            L+FSF++ +  P L  RLG+    N  WC  F     ++ + L                 
Sbjct: 2854 LMFSFSDQEGSPALVLRLGKAYGSNNMWCKSFSTHKDLADRDL------RAENTKGSYAI 2907

Query: 765  XXSVRAGRGRYRRTNIVTLAPRYQLHNNTTRTLQFAQACAATTLD 809
              SVR GRG Y  T  VTL+PR+ LHN +   L+F Q C     D
Sbjct: 2908 GISVRRGRGLYACTTFVTLSPRFHLHNRSGYKLEFMQLCDIVNYD 2952



 Score = 57.2 bits (132), Expect = 1e-07
 Identities = 25/69 (36%), Positives = 35/69 (50%)

Query: 134  DYYNRTLSSWEPMVEPWRFXXXXXXXXXXXXXXXXAQVHVTSAETLNLNVTSALVELLML 193
            +YYNR LS WEP+ E W                   ++ ++S + L LNVTS  +EL  +
Sbjct: 2385 NYYNRRLSGWEPVAETWESNLNWKYTKGHLDNKKRFEIGISSKQMLKLNVTSTFIELFHM 2444

Query: 194  VRANWTADY 202
            V  NWT D+
Sbjct: 2445 VLKNWTNDF 2453



 Score = 45.6 bits (103), Expect = 5e-04
 Identities = 26/101 (25%), Positives = 46/101 (45%), Gaps = 2/101 (1%)

Query: 249  RRRSPFVPYALRNHTGHKLWFTTLLTTVDEMRPTGRGDGCGPDDTWMLVRAQHTEPFSFG 308
            R+RSPF+P+AL+N +G  L F  +   + ++  +           W  V+   T+ F F 
Sbjct: 2462 RQRSPFIPFALQNLSGTPLLFKPIYAPLGDLTRSDL-QQVELIKNWYSVQPNETKTFDFS 2520

Query: 309  -GXXXXXXXXXXXXXWTVLLRLDGWDPPDPVCVDRVGLYFR 348
                             + +++ GW    P+ VD+VG++FR
Sbjct: 2521 QKSKLRHVHSHQLNLHQIFVQIHGWTLIGPISVDKVGMFFR 2561



 Score = 42.3 bits (95), Expect = 0.004
 Identities = 29/75 (38%), Positives = 41/75 (54%), Gaps = 3/75 (4%)

Query: 876  MQWDNQLLGTPSPVLLYCLPDRSGANAPALHASV-ELQRSPSDKYNAYFFRHLVVALKPL 934
            +Q DNQLL   SP+ L+     S       +A V +L+  PS   NA  F++L + LKP 
Sbjct: 3389 IQIDNQLLDAVSPIALHT--QTSNDLEETQNAVVLKLKMLPSPNKNAIIFKYLTLDLKPS 3446

Query: 935  AVRLEERLILVLWSW 949
               LEE+LIL + S+
Sbjct: 3447 TASLEEKLILKVASF 3461



 Score = 31.5 bits (68), Expect = 8.4
 Identities = 16/40 (40%), Positives = 25/40 (62%), Gaps = 2/40 (5%)

Query: 4    LNLRLSYHDMRMFASMLQSLPVQVRAA--LSGKAEEPLKK 41
            LN+RLSY D+++F+ M + LP Q      +  KA+  L+K
Sbjct: 2233 LNIRLSYVDVKLFSRMARLLPTQTSRPKNVISKADSDLEK 2272


>AE013599-453|AAF59228.1| 3242|Drosophila melanogaster CG2093-PA
            protein.
          Length = 3242

 Score = 44.4 bits (100), Expect = 0.001
 Identities = 32/128 (25%), Positives = 58/128 (45%), Gaps = 17/128 (13%)

Query: 1059 DAAVELEPFERVHVFETASYLGRQMLAHFKDELKWQAAKILGSVDFLGNPLGFVADVSEG 1118
            D    L  FER + F +   L  ++ +H+  +   Q   ++  +D LGNP G V  + +G
Sbjct: 2872 DVVFRLAFFEREYQFFSQKQLINEITSHYTGQALKQLYVLVLGLDVLGNPYGLVVGLKKG 2931

Query: 1119 VTGLLLEGNVGA-----------------LLKNVTHGISNSAAKVSESLGDGLERVVWDE 1161
            V  L  E   GA                 L  +   G + + +K++ ++G GL  + +DE
Sbjct: 2932 VEDLFYEPFQGAIQGPGEFAEGLVLGVKSLFGHTVGGAAGAVSKITGAMGKGLAALTFDE 2991

Query: 1162 AHEETRRR 1169
             +++ RR+
Sbjct: 2992 DYQKKRRQ 2999



 Score = 31.9 bits (69), Expect = 6.3
 Identities = 13/59 (22%), Positives = 33/59 (55%), Gaps = 5/59 (8%)

Query: 653  TVKKTDGMKVSVSAAYWLVNRTGLPLVYRAEGSAADAAGQFAEHELARVVAPLLFSFAE 711
            T  +   + +++ + +W++N+TG+ L Y++E ++ +      E+       P+LF+F +
Sbjct: 2263 TENRHGSLMLTLFSPFWMINKTGMMLTYKSETTSVEVLYHPPEYS-----GPILFTFRD 2316


>AE014297-4492|AAF56978.3| 3731|Drosophila melanogaster CG15523-PA
            protein.
          Length = 3731

 Score = 43.2 bits (97), Expect = 0.003
 Identities = 36/137 (26%), Positives = 58/137 (42%), Gaps = 20/137 (14%)

Query: 1055 IKFEDAAVELEPFERVHVFETASYLGRQMLAHFKDELKWQAAKILGSVDFLGNPLGFVAD 1114
            I  + + +    +ER  +       G+ +  H+     + A  ++GS++ LG+P G    
Sbjct: 3341 IALDHSPLSFSRYERQQILTVPLRFGQSLGLHYLSGAIFGAGWVVGSLEILGSPSGLARS 3400

Query: 1115 VSEGVTGL-------LLEGNVG----------ALLKNVTHGISNSAAKVSESLGDGLERV 1157
             S G+          L  G  G          +LL+NVT G  NS  K++ S+   L+R+
Sbjct: 3401 FSTGLRDFVSMPVQGLFRGPWGFLVGVTQGSASLLRNVTAGTVNSVTKLAGSVARNLDRL 3460

Query: 1158 VWDEAH---EETRRRIR 1171
              D  H    E RRR R
Sbjct: 3461 TLDAEHIELTEARRRAR 3477


  Database: fruitfly
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 24,830,863
  Number of sequences in database:  52,641
  
Lambda     K      H
   0.320    0.134    0.413 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 53,214,433
Number of Sequences: 52641
Number of extensions: 1941298
Number of successful extensions: 4086
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 4064
Number of HSP's gapped (non-prelim): 16
length of query: 1354
length of database: 24,830,863
effective HSP length: 94
effective length of query: 1260
effective length of database: 19,882,609
effective search space: 25052087340
effective search space used: 25052087340
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 68 (31.5 bits)