Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA002719-TA|BGIBMGA002719-PA|IPR008266|Tyrosine protein
kinase, active site, IPR000649|Initiation factor 2B related
(716 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC21E11.06 |tif224||translation initiation factor eIF2B delta ... 205 1e-53
SPCC11E10.07c |||translation initiation factor eIF2B alpha subun... 40 0.001
SPAC343.14c |||translation initiation factor eIF2B|Schizosacchar... 37 0.008
SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 31 0.73
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo... 27 6.8
SPAC1142.07c |vps32|snf7|vacuolar sorting protein Vps32|Schizosa... 27 9.0
SPAC20G8.01 |cdc17||ATP-dependent DNA ligase Cdc17|Schizosacchar... 27 9.0
SPAC3F10.08c |||rRNA processing protein Faf1|Schizosaccharomyces... 27 9.0
>SPAC21E11.06 |tif224||translation initiation factor eIF2B delta
subunit|Schizosaccharomyces pombe|chr 1|||Manual
Length = 467
Score = 205 bits (501), Expect = 1e-53
Identities = 109/323 (33%), Positives = 175/323 (54%), Gaps = 5/323 (1%)
Query: 390 INSNLHPAIVKLGVQLSSRVVKGSNARCIALLDALNKMVRDYVLPAKTEFSRGLESHLAS 449
I ++HPA+++LG++L++ + GSN RCI LL +++DY P T SR L +H+ S
Sbjct: 137 IPKDIHPAVIRLGLKLANYKIFGSNQRCIDLLKTFKIVIQDYQTPYGTTLSRHLTTHINS 196
Query: 450 SLEFLWTTREPCASQINAVKFFRHHLAQLPNNVDEFDAKKTLQEEIDRYIREQIEMAGEA 509
+ +L +TR S NA++F + ++ L ++ + + K+ L E+ID YIR++I +AG+
Sbjct: 197 QIAYLVSTRPLSISMGNAIRFLKLEISVLDIDLTDDEGKELLLEKIDSYIRDRIIIAGQV 256
Query: 510 ISIAVRNKISNGDTILTYGCSSLIEQILCEAWEAGLQFRTLVVGSRVSPDAAEMLRRLCA 569
I A KI +GD ILTY SS + +L A G +FR +VV SR + L+ L
Sbjct: 257 IVQAATEKIQDGDVILTYLHSSTVNDVLIHAKNVGKKFRVVVVDSRPEFEGRVCLKLLTE 316
Query: 570 RGLPCTYVDFTAVNYVMKTVSKXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVLVAC 629
G+ CTYV +A++Y+M+ V+K PV+ C
Sbjct: 317 HGIECTYVMISALSYIMQEVTKIFLGGHAMLSNGALYSRAGTSLISLLGHESNVPVIACC 376
Query: 630 HTHKFIDSLYTDAFMHNQIGDPDDLLEKSDDN-----SPLKDWRSNLNLTPLNLTYDVTP 684
++KF + + D+ ++N++ D L+ D+ L +W+S NL L+L YDVTP
Sbjct: 377 ESYKFTERIQLDSLVYNELAPGDQLVNMGVDDFEEKPGVLANWKSVKNLKLLSLKYDVTP 436
Query: 685 PCLITAVVTELAILPCTSAPVVL 707
P LIT V E+ +LP TS P ++
Sbjct: 437 PRLITVCVCEMGLLPSTSVPAII 459
>SPCC11E10.07c |||translation initiation factor eIF2B alpha
subunit|Schizosaccharomyces pombe|chr 3|||Manual
Length = 341
Score = 40.3 bits (90), Expect = 0.001
Identities = 53/245 (21%), Positives = 91/245 (37%), Gaps = 13/245 (5%)
Query: 471 FRHHLAQLPNNVDEFDAKKTLQEEIDRYIREQIEMAGEAISIAVRNKISNGDTILTYGCS 530
F+ + + ++V +F+ K E + ++ + I+ I +G ILT+G S
Sbjct: 86 FQRFVTRSLHDVGDFEQCKRHLVENGKLFIQRARACRQRIAHLGYPLIRDGSVILTHGFS 145
Query: 531 SLIEQILCEAWEAGLQFRTLVVGSRVSPDAAEMLRRLCARGLPCTYVDFTAVNYVMKTVS 590
+ +L A + ++F+ V SR S M R L +P V +AV++ M V
Sbjct: 146 RGVAAVLLAAAKRHVRFKVFVTESRPSGSGCLMTRTLKNACIPTCMVLDSAVSFTMNRVD 205
Query: 591 KXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXXPVLVACHTHKFID----SLYTDAFMHN 646
P +HKF+ S Y F
Sbjct: 206 LVLVGAEGVVENGGLINQIGTFQLAVFAKHAHKPFYAVAESHKFVRMFPLSQYDIPFSRP 265
Query: 647 --QIGDPD-DLLEKSDDNSPL-KDWRSN---LNLTPL--NLTYDVTPPCLITAVVTELAI 697
+ DP + + + P D N +N + N T DVTPP ++ ++T+L I
Sbjct: 266 ILEFDDPSPETVHPEPEPIPTPSDAIHNELIMNEEQIRNNPTLDVTPPEFVSGLITDLGI 325
Query: 698 LPCTS 702
+ S
Sbjct: 326 IDSKS 330
>SPAC343.14c |||translation initiation factor
eIF2B|Schizosaccharomyces pombe|chr 1|||Manual
Length = 393
Score = 37.1 bits (82), Expect = 0.008
Identities = 40/201 (19%), Positives = 70/201 (34%), Gaps = 10/201 (4%)
Query: 501 EQIEMAGEAISIAVRNKISNGDTILTYGCSSLIEQILCEAWEAGLQFRTLVVGS--RVSP 558
++++ I + + + + + ILT GCS +E L A + +F +V
Sbjct: 182 DELDKINTDIEVQSMDHLHSNEIILTQGCSKTVEAFLRFAAKK-RKFSVIVAEGFPNNQK 240
Query: 559 DAAEMLRRLCARGLPCTYVDFTAVNYVMKTVSKXXXXXXXXXXXXXXXXXXXXXXXXXXX 618
+ M +RL G+ T + + +M V+K
Sbjct: 241 GSHAMAKRLAQAGIDTTVISDATIFAIMSRVNKVILGTHAILGNGGLVTYSGAQLVAQAA 300
Query: 619 XXXXXPVLVACHTHKFIDSLYTDAFMHNQIGDPDDLLEKSDDNSPLKDWRSNLNLTPLNL 678
PV+V +K D Q+ PD ++ ++ D S + LN
Sbjct: 301 RHHATPVVVCSGIYKLSPVYPYDLESIIQLSSPDKIMSFNEG-----DLISRAEI--LNP 353
Query: 679 TYDVTPPCLITAVVTELAILP 699
YD PP L+ +T L P
Sbjct: 354 YYDYIPPDLVDLFITNLGGYP 374
>SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1526
Score = 30.7 bits (66), Expect = 0.73
Identities = 25/129 (19%), Positives = 50/129 (38%), Gaps = 4/129 (3%)
Query: 165 NKTTENPQLTKXXXXXXXXXXXSKPKVESTDEVDKSVVINPNAV----DISTSREQIKAD 220
+ T + QL K K + S EV++ +V N++ ++ T+ I D
Sbjct: 818 SSTQNDKQLKKRDAEIIELKYELKKQQNSKSEVERDLVETNNSLTAVENLLTTERAIALD 877
Query: 221 RXXXXXXXXXXXXXXXDGESNVQSSVKKELKEDDSVKQNEDMTVKDVVETLKNIVSVAKE 280
+ D S + + +E S+KQ + +++E + ++ E
Sbjct: 878 KEEILRRTQERLANIEDSFSETKQQNENLQRESASLKQINNELESELLEKTSKVETLLSE 937
Query: 281 VNEITTKVS 289
NE+ K+S
Sbjct: 938 QNELKEKLS 946
>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7
domain|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1811
Score = 27.5 bits (58), Expect = 6.8
Identities = 18/68 (26%), Positives = 34/68 (50%), Gaps = 3/68 (4%)
Query: 237 DGESNVQSSVKKELKEDDSVKQNEDMTVKDVVETLKNIVSVAKEVNEITTKVSALDLGGK 296
D ES ++S V E+ E DS+ + + T K + KN+ + + ++ + DL +
Sbjct: 30 DSESTIKSRVSDEIDEHDSIPEQSN-TEKSIEINDKNLEAEKDIESNLSLRPPEDDLDSR 88
Query: 297 --KQEETG 302
+ E+TG
Sbjct: 89 SIESEQTG 96
>SPAC1142.07c |vps32|snf7|vacuolar sorting protein
Vps32|Schizosaccharomyces pombe|chr 1|||Manual
Length = 222
Score = 27.1 bits (57), Expect = 9.0
Identities = 23/83 (27%), Positives = 38/83 (45%), Gaps = 9/83 (10%)
Query: 492 QEEIDRYIREQIEMAGE--------AISIAVRNKISNGDTILTYGCSSLIEQILCEAWEA 543
+E ++R I EQ E+A + A++ R K+ + + G + IEQ L A
Sbjct: 35 EEVLERQIAEQTEIARKNATTNKRLALTALKRKKMHENELVKIEGSRNNIEQQLFSIQNA 94
Query: 544 GLQFRTLVVGSRVSPDAAEMLRR 566
L F TL R +A + ++R
Sbjct: 95 NLNFETL-QAMRQGAEAMKSIQR 116
>SPAC20G8.01 |cdc17||ATP-dependent DNA ligase
Cdc17|Schizosaccharomyces pombe|chr 1|||Manual
Length = 768
Score = 27.1 bits (57), Expect = 9.0
Identities = 19/57 (33%), Positives = 27/57 (47%), Gaps = 1/57 (1%)
Query: 237 DGESNVQSSVKKELK-EDDSVKQNEDMTVKDVVETLKNIVSVAKEVNEITTKVSALD 292
DG S + + +K D+S N D T VE NIVS AK+ + + S+ D
Sbjct: 55 DGRSTSEKRKVESVKLVDESKHNNHDDTGTQNVERENNIVSEAKKQKTLGSSSSSSD 111
>SPAC3F10.08c |||rRNA processing protein Faf1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 259
Score = 27.1 bits (57), Expect = 9.0
Identities = 14/47 (29%), Positives = 25/47 (53%)
Query: 237 DGESNVQSSVKKELKEDDSVKQNEDMTVKDVVETLKNIVSVAKEVNE 283
+ E + + ++E K +QN+D + D VE LKN + + K + E
Sbjct: 101 EDEEEILAKKREEQKLRKRSRQNDDGSDDDEVENLKNDLELQKLLRE 147
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.131 0.377
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,658,802
Number of Sequences: 5004
Number of extensions: 95871
Number of successful extensions: 304
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 296
Number of HSP's gapped (non-prelim): 10
length of query: 716
length of database: 2,362,478
effective HSP length: 78
effective length of query: 638
effective length of database: 1,972,166
effective search space: 1258241908
effective search space used: 1258241908
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 57 (27.1 bits)
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