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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA002675-TA|BGIBMGA002675-PA|IPR002048|Calcium-binding
EF-hand, IPR001611|Leucine-rich repeat
         (703 letters)

Database: bee 
           429 sequences; 140,377 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor pr...    38   3e-04
AF084556-1|AAC71015.1|  652|Apis mellifera pipsqueak protein.          27   0.39 
AF134817-1|AAD40233.1|  105|Apis mellifera FABP-like protein pro...    25   2.1  
AB083011-1|BAC54132.1|  135|Apis mellifera fatty acid binding pr...    25   2.1  
Z26318-1|CAA81227.1|  544|Apis mellifera royal jelly protein RJP...    24   3.7  
EF531707-1|ABP57431.1|  138|Apis mellifera structural cuticle pr...    23   6.4  
AF023666-1|AAC14552.1|  363|Apis mellifera sn-glycerol-3-phospha...    23   8.5  

>AY937243-1|AAX33677.1| 1370|Apis mellifera Toll-like receptor
           protein.
          Length = 1370

 Score = 37.9 bits (84), Expect = 3e-04
 Identities = 33/105 (31%), Positives = 55/105 (52%), Gaps = 6/105 (5%)

Query: 61  INILDLSHNNITKL-NHELDRLTEVVTLDLSTNGIQNLNKFLHNAKKLVH-LNLANNRIK 118
           + ILDL +N+I ++ ++    L  + TL+LS N ++ +   L N   +++ L L+ N I 
Sbjct: 361 LQILDLRNNSIDRIESNAFLPLYNLHTLELSDNKLRTVGAQLFNGLFVLNRLTLSGNAIA 420

Query: 119 E---LAMSHLPASVSSLDLTNNLLRDVPSDLGHLTSLEHLELEGN 160
               LA  +  + +  LDL+ N L  VP  L  L  L+ L+L  N
Sbjct: 421 SIDPLAFRNC-SDLKELDLSGNELTSVPDALRDLALLKTLDLGEN 464



 Score = 36.7 bits (81), Expect = 6e-04
 Identities = 33/101 (32%), Positives = 45/101 (44%), Gaps = 4/101 (3%)

Query: 64  LDLSHNNITKLNHELDRLTEVVTLDLSTNGIQNL-NKFLHNAKKLVHLNLANNRIKELAM 122
           LDLS N +T +   L  L  + TLDL  N I N  N    N  +L  L L  N I  L+ 
Sbjct: 436 LDLSGNELTSVPDALRDLALLKTLDLGENRISNFYNGSFRNLDQLTGLRLIGNDIGNLSR 495

Query: 123 SHL--PASVSSLDLTNNLLRDVPS-DLGHLTSLEHLELEGN 160
             L    ++  L+L  N ++ V          LE + L+GN
Sbjct: 496 GMLWDLPNLQILNLARNKVQHVERYAFERNMRLEAIRLDGN 536



 Score = 36.7 bits (81), Expect = 6e-04
 Identities = 19/84 (22%), Positives = 42/84 (50%), Gaps = 1/84 (1%)

Query: 60  NINILDLSHNNITKLN-HELDRLTEVVTLDLSTNGIQNLNKFLHNAKKLVHLNLANNRIK 118
           N+ IL+L+ N +  +  +  +R   +  + L  N + ++N    +   L+ LNL+ N I+
Sbjct: 503 NLQILNLARNKVQHVERYAFERNMRLEAIRLDGNFLSDINGVFTSIASLLLLNLSENHIE 562

Query: 119 ELAMSHLPASVSSLDLTNNLLRDV 142
               + +P ++  LD+  N +  +
Sbjct: 563 WFDYAFIPGNLKWLDIHGNFIESL 586



 Score = 34.7 bits (76), Expect = 0.003
 Identities = 28/103 (27%), Positives = 53/103 (51%), Gaps = 3/103 (2%)

Query: 63  ILDLSHNNITKLNHELDRLTEVVTLDLSTNGIQNL-NKFLHNAKKLVHLNLANNRIKELA 121
           +L+LS N+I   ++       +  LD+  N I++L N +     K+  L+ ++NRI EL+
Sbjct: 553 LLNLSENHIEWFDYAFIP-GNLKWLDIHGNFIESLGNYYKIRDSKVKTLDASHNRITELS 611

Query: 122 MSHLPASVSSLDLTNNLLRDV-PSDLGHLTSLEHLELEGNPLD 163
              +P SV  L + NN +  V P+      +L  +++  N ++
Sbjct: 612 PLSVPDSVELLFINNNYINLVRPNTFTDKVNLTRVDMYANMIE 654



 Score = 33.5 bits (73), Expect = 0.006
 Identities = 35/109 (32%), Positives = 48/109 (44%), Gaps = 6/109 (5%)

Query: 60  NINILDLSHNNITKL--NHELDRLTEVVTLDLSTNGIQNL-NKFLHNAKKLVHLNLANNR 116
           +I ILDLS N IT+L  N  L  L ++  L L  N I  +    L     L   N + N 
Sbjct: 213 DIRILDLSRNEITRLQENSPLLDLRQLQELHLQRNAIVEIAGDALTGLTVLRTFNASYNS 272

Query: 117 IKELAMSHLPAS--VSSLDLTNNLLRDVPSDL-GHLTSLEHLELEGNPL 162
           +  L      ++  +  + L  N LRD+P  +   L  L  L L GN L
Sbjct: 273 LDSLPEGLFASTRDLREIHLAYNGLRDLPKGIFTRLEQLLVLNLAGNRL 321



 Score = 27.9 bits (59), Expect = 0.30
 Identities = 27/91 (29%), Positives = 45/91 (49%), Gaps = 6/91 (6%)

Query: 61  INILDLSHNNITKLNHELDR-LTEVVTLDLSTNGIQNLNKFLHNAKKLVHLNLANNRIKE 119
           +N L LS N I  ++    R  +++  LDLS N + ++   L +   L  L+L  NRI  
Sbjct: 409 LNRLTLSGNAIASIDPLAFRNCSDLKELDLSGNELTSVPDALRDLALLKTLDLGENRIS- 467

Query: 120 LAMSHLPASVSSLDLTNNLLRDVPSDLGHLT 150
              +    S  +LD     LR + +D+G+L+
Sbjct: 468 ---NFYNGSFRNLDQLTG-LRLIGNDIGNLS 494


>AF084556-1|AAC71015.1|  652|Apis mellifera pipsqueak protein.
          Length = 652

 Score = 27.5 bits (58), Expect = 0.39
 Identities = 18/65 (27%), Positives = 26/65 (40%), Gaps = 2/65 (3%)

Query: 614 NDDENPRLNGTNDSDTSQPLINGDQNTDSDFLSPSREYVPVYSPDMGRVRIKMTETPKPK 673
           N++ N   NG ND+       N + N D    +    Y   + PD G V +     P P 
Sbjct: 244 NNNNNNNNNGANDNGNGNGASNNNNNGDMFCHTGLGHY--GHHPDPGEVDLPPETQPTPP 301

Query: 674 TPVLV 678
           +  LV
Sbjct: 302 SATLV 306



 Score = 23.4 bits (48), Expect = 6.4
 Identities = 9/35 (25%), Positives = 15/35 (42%)

Query: 542 NGDVANGINHAENNDPKTESPRDTNQNRSSSKDNN 576
           N + +N  N+  NN+       D      +S +NN
Sbjct: 234 NTNASNNNNNNNNNNNNNNGANDNGNGNGASNNNN 268


>AF134817-1|AAD40233.1|  105|Apis mellifera FABP-like protein
           protein.
          Length = 105

 Score = 25.0 bits (52), Expect = 2.1
 Identities = 20/76 (26%), Positives = 32/76 (42%), Gaps = 3/76 (3%)

Query: 525 GKYELVPTHELHQKKSENGDVANGINHAENNDPKTESPRDTNQ--NRSSSKDNNLAKTPS 582
           GK++ V  +   +     GD  N +N      P  E  ++ ++    SSS DN   KT  
Sbjct: 4   GKFQFVSQNNFEEFAKVLGD-QNLVNTVLQPRPSFELSKNGDEWTFTSSSGDNTYTKTFK 62

Query: 583 QQETSFESAPASPDSR 598
                 E+ P+ PD +
Sbjct: 63  MNVPFEETLPSLPDRK 78


>AB083011-1|BAC54132.1|  135|Apis mellifera fatty acid binding
           protein protein.
          Length = 135

 Score = 25.0 bits (52), Expect = 2.1
 Identities = 20/76 (26%), Positives = 32/76 (42%), Gaps = 3/76 (3%)

Query: 525 GKYELVPTHELHQKKSENGDVANGINHAENNDPKTESPRDTNQ--NRSSSKDNNLAKTPS 582
           GK++ V  +   +     GD  N +N      P  E  ++ ++    SSS DN   KT  
Sbjct: 6   GKFQFVSQNNFEEFAKVLGD-QNLVNTVLQPRPSFELSKNGDEWTFTSSSGDNTYTKTFK 64

Query: 583 QQETSFESAPASPDSR 598
                 E+ P+ PD +
Sbjct: 65  MNVPFEETLPSLPDRK 80


>Z26318-1|CAA81227.1|  544|Apis mellifera royal jelly protein
           RJP57-1 protein.
          Length = 544

 Score = 24.2 bits (50), Expect = 3.7
 Identities = 14/83 (16%), Positives = 32/83 (38%)

Query: 546 ANGINHAENNDPKTESPRDTNQNRSSSKDNNLAKTPSQQETSFESAPASPDSRKDNNSLS 605
           AN  N    ND +    +     ++ +K N   +  ++Q  + ++     D++++ N  +
Sbjct: 452 ANKQNGNRQNDNRQNDNKQNGNRQNDNKQNGNRQNDNKQNGNRQNGNKQNDNKQNGNRQN 511

Query: 606 SEDLFVPLNDDENPRLNGTNDSD 628
                    +D     N  N +D
Sbjct: 512 DNKRNGNRQNDNQNNQNDNNRND 534


>EF531707-1|ABP57431.1|  138|Apis mellifera structural cuticle
           protein protein.
          Length = 138

 Score = 23.4 bits (48), Expect = 6.4
 Identities = 8/17 (47%), Positives = 12/17 (70%)

Query: 542 NGDVANGINHAENNDPK 558
           N + +NGI+H E+  PK
Sbjct: 45  NFETSNGISHQESGQPK 61


>AF023666-1|AAC14552.1|  363|Apis mellifera sn-glycerol-3-phosphate
           dehydrogenase protein.
          Length = 363

 Score = 23.0 bits (47), Expect = 8.5
 Identities = 13/49 (26%), Positives = 25/49 (51%), Gaps = 1/49 (2%)

Query: 429 VTAVSEVL-GQRPADKDETPTPHKTGTYVCIAIIVMLLVSLIGFAIIKG 476
           + A+ +V+   + AD      PH+    +C A+   +  + IG ++IKG
Sbjct: 74  IIAIPDVVEAAKDADILTFVVPHQFIKRICSALFGKIKPTAIGLSLIKG 122


  Database: bee
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 140,377
  Number of sequences in database:  429
  
Lambda     K      H
   0.311    0.130    0.371 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 195,467
Number of Sequences: 429
Number of extensions: 8529
Number of successful extensions: 29
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 18
Number of HSP's gapped (non-prelim): 14
length of query: 703
length of database: 140,377
effective HSP length: 63
effective length of query: 640
effective length of database: 113,350
effective search space: 72544000
effective search space used: 72544000
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.8 bits)
S2: 47 (23.0 bits)

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