Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA002644-TA|BGIBMGA002644-PA|IPR000308|14-3-3 protein
(313 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 258 1e-69
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 247 1e-66
SPBC15C4.04c |||amino acid permease, unknown 10|Schizosaccharomy... 31 0.16
SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces... 30 0.37
SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pomb... 30 0.49
SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1 ... 30 0.49
SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regula... 29 0.65
SPAC2F3.12c |||conserved eukaryotic protein|Schizosaccharomyces ... 27 3.4
SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pomb... 27 3.4
SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl cis-... 27 4.6
SPBC839.10 |usp107|snu71|U1 snRNP-associated protein Usp107|Schi... 27 4.6
SPCC188.13c |dcr1|SPCC584.10c|dicer|Schizosaccharomyces pombe|ch... 27 4.6
SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|ch... 26 8.0
SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase Cdc48|Schizosac... 26 8.0
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 258 bits (631), Expect = 1e-69
Identities = 130/216 (60%), Positives = 160/216 (74%), Gaps = 3/216 (1%)
Query: 2 SVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSW 61
+ +E+ V AKLAEQAERY+ M MK V T EL+ EERNLLSVAYKNV+GARR+SW
Sbjct: 3 TTSREDAVYLAKLAEQAERYEGMVENMKSVASTDQELTVEERNLLSVAYKNVIGARRASW 62
Query: 62 RVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKVF 119
R++SSIEQK E G+ + ++ KEYR K+E+EL IC D+L +L+KHLIP A++ ESKVF
Sbjct: 63 RIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEKHLIPNAASAESKVF 122
Query: 120 YLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSVF 179
Y KMKGDYYRYLAE A GE R + S + Y+ A EI+ A++ PTHPIRLGLALNFSVF
Sbjct: 123 YYKMKGDYYRYLAEFAVGEKRQHSADQSLEGYKAASEIATAELAPTHPIRLGLALNFSVF 182
Query: 180 YYEILNSPDKACQLAKQESRINTSRLSPAVVEDSQK 215
YYEILNSPD+AC LAKQ S L ++ E+S K
Sbjct: 183 YYEILNSPDRACYLAKQAFDEAISELD-SLSEESYK 217
Score = 148 bits (358), Expect = 1e-36
Identities = 79/136 (58%), Positives = 98/136 (72%), Gaps = 6/136 (4%)
Query: 166 HPIRLGLALNFSVFYYEILNSPDKACQLAKQESRINTSRLSPAVVEDSQKAYQEAFDIAK 225
H I + VFYY++ D LA E + R A + S + Y+ A +IA
Sbjct: 109 HLIPNAASAESKVFYYKMKG--DYYRYLA--EFAVGEKRQHSA--DQSLEGYKAASEIAT 162
Query: 226 AKMQPSHPIRLGLALNFSVFYYEIINSPARACHLAKQAFDDAIAELDTLNEDSYKDSTLI 285
A++ P+HPIRLGLALNFSVFYYEI+NSP RAC+LAKQAFD+AI+ELD+L+E+SYKDSTLI
Sbjct: 163 AELAPTHPIRLGLALNFSVFYYEILNSPDRACYLAKQAFDEAISELDSLSEESYKDSTLI 222
Query: 286 MQLLRDNLTLWTSDTQ 301
MQLLRDNLTLWTSD +
Sbjct: 223 MQLLRDNLTLWTSDAE 238
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 247 bits (605), Expect = 1e-66
Identities = 122/198 (61%), Positives = 151/198 (76%), Gaps = 2/198 (1%)
Query: 1 MSVDKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSS 60
MS +E V AKLAEQAERY++M MK+V + +LS EERNLLSVAYKN++GARR+S
Sbjct: 1 MSNSRENSVYLAKLAEQAERYEEMVENMKKVACSNDKLSVEERNLLSVAYKNIIGARRAS 60
Query: 61 WRVISSIEQKTE--GSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPKASNPESKV 118
WR+ISSIEQK E G+ R+ + KEYR K+E EL +IC+DVL +L+KHLIP A+ ESKV
Sbjct: 61 WRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAATTGESKV 120
Query: 119 FYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAFEISKAKMQPTHPIRLGLALNFSV 178
FY KMKGDYYRYLAE GE + S +AY+ A +I+ A++ PT P+RLGLALNFSV
Sbjct: 121 FYYKMKGDYYRYLAEFTVGEVCKEAADSSLEAYKAASDIAVAELPPTDPMRLGLALNFSV 180
Query: 179 FYYEILNSPDKACQLAKQ 196
FYYEIL+SP+ AC LAKQ
Sbjct: 181 FYYEILDSPESACHLAKQ 198
Score = 142 bits (344), Expect = 6e-35
Identities = 65/91 (71%), Positives = 80/91 (87%)
Query: 211 EDSQKAYQEAFDIAKAKMQPSHPIRLGLALNFSVFYYEIINSPARACHLAKQAFDDAIAE 270
+ S +AY+ A DIA A++ P+ P+RLGLALNFSVFYYEI++SP ACHLAKQ FD+AI+E
Sbjct: 147 DSSLEAYKAASDIAVAELPPTDPMRLGLALNFSVFYYEILDSPESACHLAKQVFDEAISE 206
Query: 271 LDTLNEDSYKDSTLIMQLLRDNLTLWTSDTQ 301
LD+L+E+SYKDSTLIMQLLRDNLTLWTSD +
Sbjct: 207 LDSLSEESYKDSTLIMQLLRDNLTLWTSDAE 237
>SPBC15C4.04c |||amino acid permease, unknown
10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 542
Score = 31.5 bits (68), Expect = 0.16
Identities = 21/67 (31%), Positives = 36/67 (53%), Gaps = 4/67 (5%)
Query: 2 SVDKEELVQRAKLAEQAERYDDMAAAMKEVT--ETGVELSNEERN-LLSVAYKNVVGARR 58
SV + ++ K ++ E + ++ + +K V+ ET E+SN+E N LL + YK V
Sbjct: 3 SVSNVSVNEQGKFNDKEEGFSNLKS-LKHVSHSETDFEVSNDEDNQLLELGYKPVFKREF 61
Query: 59 SSWRVIS 65
S+W S
Sbjct: 62 STWATFS 68
>SPBC16C6.02c |vps1302|vps13b|chorein homolog|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 3131
Score = 30.3 bits (65), Expect = 0.37
Identities = 21/91 (23%), Positives = 39/91 (42%), Gaps = 1/91 (1%)
Query: 6 EELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRVIS 65
EEL+ R + + Y+ MA + E E ++ + LLS Y N +R+ +
Sbjct: 3011 EELM-RERFEHLLKAYEKMALMVAEQEEFNAKIEDMALKLLSEKYDNEAYQAELFYRLSN 3069
Query: 66 SIEQKTEGSERKQQMAKEYRVKVEKELREIC 96
+E+ + EY +E+ L++ C
Sbjct: 3070 CVEKVLHNKISITDLKTEYEEILEQTLKKEC 3100
>SPBPJ4664.04 |||coatomer alpha subunit |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1207
Score = 29.9 bits (64), Expect = 0.49
Identities = 37/137 (27%), Positives = 65/137 (47%), Gaps = 9/137 (6%)
Query: 45 LLSVAYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYR-VKVEKELR-EICYDVLGL 102
L+S +N A RS + + I + S + ++ + YR VK K L +IC+ +
Sbjct: 968 LVSYVRRNAETAERS--QALPFITRNL-ASIKSHELHEAYRLVKANKILEAQICFRSIIY 1024
Query: 103 LDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATG-ETRHSVVEDSQKAYQDAFEISKAK 161
L L A++ E + + RY+ ++ E R ED+++A + ++ + A
Sbjct: 1025 LA--LTTVANSEEEADEISALIDECCRYIVALSCELERRRLGEEDTKRALELSYYFASAD 1082
Query: 162 MQPTHP-IRLGLALNFS 177
+QP H I L LA+N S
Sbjct: 1083 LQPMHSIIALRLAINAS 1099
Score = 27.1 bits (57), Expect = 3.4
Identities = 14/34 (41%), Positives = 22/34 (64%), Gaps = 1/34 (2%)
Query: 211 EDSQKAYQEAFDIAKAKMQPSHP-IRLGLALNFS 243
ED+++A + ++ A A +QP H I L LA+N S
Sbjct: 1066 EDTKRALELSYYFASADLQPMHSIIALRLAINAS 1099
>SPAC29E6.03c |uso1|SPAC30.07c|ER to Golgi tethering factor Uso1
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1044
Score = 29.9 bits (64), Expect = 0.49
Identities = 23/91 (25%), Positives = 46/91 (50%), Gaps = 5/91 (5%)
Query: 4 DKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSV--AYKNVVGARRSSW 61
+KE+ V K E ++ +A A +++ E+ + +++ + A N+ + S
Sbjct: 925 NKEKSVNNEKFKEVSQA---LAEANEKLNARDEEIERLKVDIIGLQNASLNMQSLKDSDN 981
Query: 62 RVISSIEQKTEGSERKQQMAKEYRVKVEKEL 92
R IS +E K + E+K + A EY + + +EL
Sbjct: 982 RTISDLESKNKELEKKLKEADEYWLLIVEEL 1012
>SPCC188.11 |prp45|cwf13, snw1, SPCC584.08|transcriptional regulator
Prp45|Schizosaccharomyces pombe|chr 3|||Manual
Length = 557
Score = 29.5 bits (63), Expect = 0.65
Identities = 27/93 (29%), Positives = 45/93 (48%), Gaps = 8/93 (8%)
Query: 4 DKEELVQRAKLAEQAERYDDMAAAMKEVTETGVELSNEERNLLSVAYKNVVGARRSSWRV 63
+K+E QR + Q R D M + +G S+ + SV+ + +R S+
Sbjct: 320 EKQEKEQRLFMLAQKAREDRMG---RNAASSGP--SHAKPRSTSVSSEERSRSRAGSFSH 374
Query: 64 ISSIEQKTEGSE---RKQQMAKEYRVKVEKELR 93
S E + E SE R+Q++ +E R + EK+LR
Sbjct: 375 HSESENEDEDSEAFRRRQELRRERRRQAEKDLR 407
>SPAC2F3.12c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 279
Score = 27.1 bits (57), Expect = 3.4
Identities = 13/40 (32%), Positives = 21/40 (52%)
Query: 253 PARACHLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDN 292
PA C++ Q D I D N+D ++ S L +LL+ +
Sbjct: 208 PAVLCYVNSQLVDKIIGFADLGNKDDFETSLLEFRLLKSS 247
>SPAC1486.05 |nup189||nucleoporin Nup189|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1778
Score = 27.1 bits (57), Expect = 3.4
Identities = 15/49 (30%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Query: 102 LLDKHLIPKASNPESKVFYLKMKGDYYR---YLAEVATGETRHSVVEDS 147
+++K IP++ E+K Y + GD+ +L E A E H V+ D+
Sbjct: 1608 MIEKLCIPESWLNEAKALYARYVGDHLNELYFLQEAALYEDAHKVLLDT 1656
>SPBC16H5.05c |cyp7|cwf27|cyclophilin family peptidyl-prolyl
cis-trans isomerase Cyp7|Schizosaccharomyces pombe|chr
2|||Manual
Length = 463
Score = 26.6 bits (56), Expect = 4.6
Identities = 12/39 (30%), Positives = 20/39 (51%)
Query: 51 KNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVE 89
KN+ S+ R +SS + K +E + M +Y K+E
Sbjct: 350 KNLENDEESTLRALSSFQSKIRNAEDEDVMDSQYGSKIE 388
>SPBC839.10 |usp107|snu71|U1 snRNP-associated protein
Usp107|Schizosaccharomyces pombe|chr 2|||Manual
Length = 695
Score = 26.6 bits (56), Expect = 4.6
Identities = 28/110 (25%), Positives = 47/110 (42%), Gaps = 8/110 (7%)
Query: 4 DKEELVQRAKLAEQA-ERYDDMAAAMK--EVTETGVELSNEERNLLSVAYKNVVGARRSS 60
D ++RA A QA E+ + + +K E+ +L LL V + + R S
Sbjct: 265 DVRSRIERA--ARQAREKNEKLLQNVKTSEIPINAADLEGINPELLPVIEEEIRSFRDQS 322
Query: 61 WRVISSIEQKTEGSERKQQMAKEYRVKVEKELREICYDVLGLLDKHLIPK 110
+K + + KEY K +++LR+ D+ LL KH I +
Sbjct: 323 ---AMKKREKQRSKDEYASLYKEYTRKEQEKLRKQNDDLQNLLSKHRISR 369
>SPCC188.13c |dcr1|SPCC584.10c|dicer|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1374
Score = 26.6 bits (56), Expect = 4.6
Identities = 19/67 (28%), Positives = 28/67 (41%)
Query: 219 EAFDIAKAKMQPSHPIRLGLALNFSVFYYEIINSPARACHLAKQAFDDAIAELDTLNEDS 278
+A + A+ P+ IR + ++I HL F IA+L+TL ED
Sbjct: 578 KACKVPAAQGSPAKSIRKAKQNAAFIMCLDLIRMGLIDKHLKPLDFRRKIADLETLEEDE 637
Query: 279 YKDSTLI 285
KD I
Sbjct: 638 LKDEGYI 644
>SPAC323.04 |||mitochondrial ATPase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 487
Score = 25.8 bits (54), Expect = 8.0
Identities = 12/45 (26%), Positives = 22/45 (48%)
Query: 47 SVAYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKE 91
S +Y + G S W+ I ++ K+ G +R ++ Y +KE
Sbjct: 61 SFSYPFLKGKSDSPWQAIQLLDFKSSGQQRAAYYSERYHSFRDKE 105
>SPAC1565.08 |cdc48|SPAC6F12.01|AAA family ATPase
Cdc48|Schizosaccharomyces pombe|chr 1|||Manual
Length = 815
Score = 25.8 bits (54), Expect = 8.0
Identities = 30/125 (24%), Positives = 60/125 (48%), Gaps = 14/125 (11%)
Query: 36 VELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTEGSERKQQMAKEYRVKVEKELREI 95
+ +EE +L V Y ++ G RR ++ I + E R Q+ K +K + + +
Sbjct: 209 INREDEESSLAEVGYDDIGGCRRQ----MAQIRELVELPLRHPQLFKSIGIKPPRGI--L 262
Query: 96 CYDVLGLLDKHLIPKASNPESKVFYLKMKGDYYRYLAEVATGETRHSVVEDSQKAYQDAF 155
Y G K L+ +A E+ F+ + G ++++A GE+ ++ +KA+++A
Sbjct: 263 MYGPPG-TGKTLMARAVANETGAFFFLINGP--EIMSKMA-GESESNL----RKAFEEAE 314
Query: 156 EISKA 160
+ S A
Sbjct: 315 KNSPA 319
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.313 0.129 0.355
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,405,191
Number of Sequences: 5004
Number of extensions: 58659
Number of successful extensions: 194
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 180
Number of HSP's gapped (non-prelim): 17
length of query: 313
length of database: 2,362,478
effective HSP length: 73
effective length of query: 240
effective length of database: 1,997,186
effective search space: 479324640
effective search space used: 479324640
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
S2: 54 (25.8 bits)
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