Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA002569-TA|BGIBMGA002569-PA|IPR000886|Endoplasmic
reticulum targeting sequence, IPR006662|Thioredoxin-related,
IPR012336|Thioredoxin-like fold, IPR005788|Disulphide isomerase,
IPR013766|Thioredoxin domain
(419 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces ... 122 7e-29
SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces po... 86 1e-17
SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces po... 80 7e-16
SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces ... 50 8e-07
SPBC12D12.07c |trx2||mitochondrial thioredoxin Trx2|Schizosaccha... 49 1e-06
SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyc... 46 8e-06
SPBC577.08c |txl1|trx3|thioredoxin-like I protein Txl1|Schizosac... 40 7e-04
SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|c... 40 9e-04
SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|ch... 32 0.17
SPBC211.01 |rsm10|SPBC23E6.11|mitochondrial ribosomal protein su... 31 0.40
SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces ... 29 0.92
>SPAC17H9.14c |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 359
Score = 122 bits (295), Expect = 7e-29
Identities = 78/250 (31%), Positives = 117/250 (46%), Gaps = 19/250 (7%)
Query: 18 SSDVIELTPSN-FDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALK--GIVKVGA 74
+S V+EL N + + S + +IEF+A WCGHCKSL P Y++ + V +G
Sbjct: 19 ASGVVELQSLNELENTIRASKKGALIEFYATWCGHCKSLAPVYEELGALFEDHNDVLIGK 78
Query: 75 LDADEHRSVSQKYGVTGFPTIKIF--TGSKHTPYQGQRTAEGFVXXXXXXXXXXXXXNLX 132
+DAD H V+ KY +TGFPT+ F GS+ Y R +
Sbjct: 79 IDADTHSDVADKYHITGFPTLIWFPPDGSEPVQYSNARDVDSL---------TQFVSEKT 129
Query: 133 XXXXXXXXXXXXVITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELKG 192
V+ L NF ++V+D LVEFYA WCG+CK L P + K
Sbjct: 130 GIKKRKIVLPSNVVELDSLNFDKVVMDDKKDVLVEFYADWCGYCKRLAPTYETLGKVFKN 189
Query: 193 K--VKLGALDATVHTTMASRYQVQGYPTIKLFPSGKKSSDSAEDYNGGRTSSDIVTWALE 250
+ V++ ++A V + ++V +PTIK FP K D E Y G R+ ++ + +
Sbjct: 190 EPNVEIVKINADVFADIGRLHEVASFPTIKFFP--KDDKDKPELYEGDRSLESLIEY-IN 246
Query: 251 KLAENVPAPD 260
K + +PD
Sbjct: 247 KKSGTQRSPD 256
>SPAC13F5.05 |||thioredoxin family protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 363
Score = 85.8 bits (203), Expect = 1e-17
Identities = 47/114 (41%), Positives = 66/114 (57%), Gaps = 4/114 (3%)
Query: 146 ITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKLGAL--DATV 203
I L NF++ V LV FYAPWCG+CK L P + K A+ L + + A+ DA
Sbjct: 34 IELNSKNFRKFVKAKGPS-LVVFYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCDADQ 92
Query: 204 HTTMASRYQVQGYPTIKL-FPSGKKSSDSAEDYNGGRTSSDIVTWALEKLAENV 256
+ + S+YQVQG+PTIKL +PS K SS S+ DYNG R+ + + + + V
Sbjct: 93 NRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRSYKSLQKFVSDSIPSKV 146
Score = 79.0 bits (186), Expect = 1e-15
Identities = 44/101 (43%), Positives = 60/101 (59%), Gaps = 9/101 (8%)
Query: 19 SDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGAL--D 76
S+ IEL NF K V ++ F+APWCG+CK LVP Y+K A L ++ V A+ D
Sbjct: 31 SNTIELNSKNFRKFVKAKGPSLVV-FYAPWCGYCKKLVPTYQKLASNLHSLLPVTAVDCD 89
Query: 77 ADEHRSVSQKYGVTGFPTIK-IFTGSK-----HTPYQGQRT 111
AD++R+V +Y V GFPTIK ++ SK T Y G R+
Sbjct: 90 ADQNRAVCSQYQVQGFPTIKLVYPSSKGSSLSSTDYNGDRS 130
>SPAC1F5.02 |||protein disulfide isomerase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 492
Score = 79.8 bits (188), Expect = 7e-16
Identities = 49/134 (36%), Positives = 68/134 (50%), Gaps = 15/134 (11%)
Query: 145 VITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELK--GKVKLGALDAT 202
++ L NF ++V+D LVEFYAPWCGHCKNL P + K A E V + +DAT
Sbjct: 357 LVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVAKIDAT 416
Query: 203 VHTTMASRYQVQGYPTIKLFPSGKKSSDSAEDYNGGRTSSDIVTW--------ALEKLAE 254
+ S + G+PTI F + K + Y G RT D+ + ++K E
Sbjct: 417 ENDISVS---ISGFPTIMFFKANDKVNPVR--YEGDRTLEDLSAFIDKHASFEPIKKEKE 471
Query: 255 NVPAPDIIQVVGEE 268
+VPAPD+ V E
Sbjct: 472 SVPAPDLEDQVAVE 485
Score = 78.6 bits (185), Expect = 2e-15
Identities = 38/101 (37%), Positives = 59/101 (58%), Gaps = 5/101 (4%)
Query: 157 VLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATEL-KGKVKLGALDATVHTTMASRYQVQG 215
++ +D + +V+FYAPWCGHCK L P + AA EL K + L +D T + S Y ++G
Sbjct: 35 LITADKVLMVKFYAPWCGHCKALAPEYESAADELEKDGISLVEVDCTEEGDLCSEYSIRG 94
Query: 216 YPTIKLFPSGKKSSDSAEDYNGGRTSSDIVTWALEKLAENV 256
YPT+ +F +GK+ S Y+G R +V + ++L V
Sbjct: 95 YPTLNVFKNGKQIS----QYSGPRKHDALVKYMRKQLLPTV 131
Score = 77.8 bits (183), Expect = 3e-15
Identities = 37/118 (31%), Positives = 69/118 (58%), Gaps = 5/118 (4%)
Query: 1 MLGILLCATGSLALYDSSSDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYK 60
+L L +G + +S++V ++ ++L+T +D++ +++F+APWCGHCK+L PEY+
Sbjct: 6 LLAAFLAFSGGF--FCASAEVPKVNKEGLNELIT-ADKVLMVKFYAPWCGHCKALAPEYE 62
Query: 61 KAARAL-KGIVKVGALDADEHRSVSQKYGVTGFPTIKIF-TGSKHTPYQGQRTAEGFV 116
AA L K + + +D E + +Y + G+PT+ +F G + + Y G R + V
Sbjct: 63 SAADELEKDGISLVEVDCTEEGDLCSEYSIRGYPTLNVFKNGKQISQYSGPRKHDALV 120
Score = 74.9 bits (176), Expect = 2e-14
Identities = 41/103 (39%), Positives = 58/103 (56%), Gaps = 8/103 (7%)
Query: 16 DSSSDVIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALK--GIVKVG 73
+S D++ L NFD +V + + ++EF+APWCGHCK+L P Y+K A V V
Sbjct: 352 ESQEDLVVLVADNFDDIVMDETKDVLVEFYAPWCGHCKNLAPTYEKLAEEYSDDSNVVVA 411
Query: 74 ALDADEHRSVSQKYGVTGFPTIKIF-TGSKHTP--YQGQRTAE 113
+DA E+ +S ++GFPTI F K P Y+G RT E
Sbjct: 412 KIDATEN-DIS--VSISGFPTIMFFKANDKVNPVRYEGDRTLE 451
>SPBC3D6.13c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 726
Score = 49.6 bits (113), Expect = 8e-07
Identities = 24/114 (21%), Positives = 50/114 (43%), Gaps = 5/114 (4%)
Query: 155 ELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKLGALDATVHTTMASRYQVQ 214
+ L + W ++FY+ C C ++ W A ++GK+ + ++ V +Y +Q
Sbjct: 292 DAALTDKEGWFIQFYSSECDDCDDVSTAWYAMANRMRGKLNVAHINCAVSKRACKQYSIQ 351
Query: 215 GYPTIKLFPSGKKSSDSAEDYNGGRTSSDIVTWALEKLAENVPAPDIIQVVGEE 268
+PT F ++ +Y G D+V++A E + +++ V E
Sbjct: 352 YFPTFLFF-----KEEAFVEYVGLPNEGDLVSFAEEAANFEIREVELLDTVNAE 400
Score = 43.2 bits (97), Expect = 7e-05
Identities = 25/86 (29%), Positives = 45/86 (52%), Gaps = 9/86 (10%)
Query: 146 ITLTDSNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHW----AKAATELKG-KVKLGALD 200
+ LTD++ + V S W +++Y P CG CK L P W KA +++G G +D
Sbjct: 29 VPLTDNDLESEV--SKGTWFIKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVD 86
Query: 201 ATVHTTMASRYQVQGYPTIKLFPSGK 226
+ ++S ++ PT+ L+ +G+
Sbjct: 87 CS--KELSSCANIRAVPTLYLYQNGE 110
Score = 37.1 bits (82), Expect = 0.005
Identities = 18/82 (21%), Positives = 36/82 (43%), Gaps = 1/82 (1%)
Query: 27 SNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQK 86
++ D +T+ E W I+F++ C C + + A ++G + V ++ + ++
Sbjct: 289 ADIDAALTDK-EGWFIQFYSSECDDCDDVSTAWYAMANRMRGKLNVAHINCAVSKRACKQ 347
Query: 87 YGVTGFPTIKIFTGSKHTPYQG 108
Y + FPT F Y G
Sbjct: 348 YSIQYFPTFLFFKEEAFVEYVG 369
Score = 30.3 bits (65), Expect = 0.53
Identities = 18/80 (22%), Positives = 35/80 (43%), Gaps = 5/80 (6%)
Query: 22 IELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGAL---DAD 78
+ LT ++ + V+ W I+++ P CG CK L P + K V+ + D
Sbjct: 29 VPLTDNDLESEVSKGT--WFIKYYLPSCGACKRLGPMWDNMVEKAKEQVEGSNFHFGEVD 86
Query: 79 EHRSVSQKYGVTGFPTIKIF 98
+ +S + PT+ ++
Sbjct: 87 CSKELSSCANIRAVPTLYLY 106
>SPBC12D12.07c |trx2||mitochondrial thioredoxin
Trx2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 121
Score = 49.2 bits (112), Expect = 1e-06
Identities = 21/64 (32%), Positives = 40/64 (62%), Gaps = 1/64 (1%)
Query: 35 NSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPT 94
++D++ +++F+A WCG CK L P +K + + + A++AD+ ++QK GV PT
Sbjct: 33 SADKVTVVDFYADWCGPCKYLKPFLEKLSEQNQKASFI-AVNADKFSDIAQKNGVYALPT 91
Query: 95 IKIF 98
+ +F
Sbjct: 92 MVLF 95
Score = 47.6 bits (108), Expect = 3e-06
Identities = 24/70 (34%), Positives = 39/70 (55%), Gaps = 1/70 (1%)
Query: 158 LDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKLGALDATVHTTMASRYQVQGYP 217
+ +D + +V+FYA WCG CK L+P K +E K A++A + +A + V P
Sbjct: 32 ISADKVTVVDFYADWCGPCKYLKPFLEK-LSEQNQKASFIAVNADKFSDIAQKNGVYALP 90
Query: 218 TIKLFPSGKK 227
T+ LF G++
Sbjct: 91 TMVLFRKGQE 100
>SPAC7D4.07c |trx1||cytosolic thioredoxin Trx1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 103
Score = 46.4 bits (105), Expect = 8e-06
Identities = 22/94 (23%), Positives = 49/94 (52%), Gaps = 2/94 (2%)
Query: 21 VIELTPSNFDKLVTNSDEIWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADEH 80
V +++ S+ K + D++ +++FFA WCG CK++ P++++ + + +D D+
Sbjct: 2 VKQVSDSSEFKSIVCQDKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFI-KVDVDQL 60
Query: 81 RSVSQKYGVTGFPTIKIF-TGSKHTPYQGQRTAE 113
++ + GV P+ ++ G K G A+
Sbjct: 61 SEIAAEAGVHAMPSFFLYKNGEKIEEIVGANPAK 94
Score = 40.3 bits (90), Expect = 5e-04
Identities = 22/77 (28%), Positives = 39/77 (50%), Gaps = 2/77 (2%)
Query: 151 SNFKELVLDSDDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKLGALDATVHTTMASR 210
S FK +V D L +V+F+A WCG CK + P + + + + +D + +A+
Sbjct: 9 SEFKSIVCQ-DKLVVVDFFATWCGPCKAIAPKFEQFSNTYSDATFI-KVDVDQLSEIAAE 66
Query: 211 YQVQGYPTIKLFPSGKK 227
V P+ L+ +G+K
Sbjct: 67 AGVHAMPSFFLYKNGEK 83
>SPBC577.08c |txl1|trx3|thioredoxin-like I protein
Txl1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 290
Score = 39.9 bits (89), Expect = 7e-04
Identities = 19/62 (30%), Positives = 30/62 (48%), Gaps = 1/62 (1%)
Query: 42 IEFFAPWCGHCKSLVPEYKKAARALKGIVKVGA-LDADEHRSVSQKYGVTGFPTIKIFTG 100
++ +A WCG CK++ P + + A V A ++ DE R ++ GV PT F
Sbjct: 24 VDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFFEN 83
Query: 101 SK 102
K
Sbjct: 84 GK 85
Score = 38.3 bits (85), Expect = 0.002
Identities = 19/63 (30%), Positives = 32/63 (50%), Gaps = 1/63 (1%)
Query: 166 VEFYAPWCGHCKNLEPHWAKAATELKG-KVKLGALDATVHTTMASRYQVQGYPTIKLFPS 224
V+ YA WCG CK + P +++ A++ K ++ +AS V+ PT F +
Sbjct: 24 VDCYADWCGPCKAISPLFSQLASKYASPKFVFAKVNVDEQRQIASGLGVKAMPTFVFFEN 83
Query: 225 GKK 227
GK+
Sbjct: 84 GKQ 86
>SPBC26H8.06 |grx4||glutaredoxin Grx4|Schizosaccharomyces pombe|chr
2|||Manual
Length = 244
Score = 39.5 bits (88), Expect = 9e-04
Identities = 20/83 (24%), Positives = 43/83 (51%), Gaps = 3/83 (3%)
Query: 22 IELT-PSNFDKLVTNSDE-IWIIEFFAPWCGHCKSLVPEYKKAARALKGIVKVGALDADE 79
+E+T F +++ N E I ++ F+APW CK + + + A+ K V + ++A++
Sbjct: 3 VEITFVEQFQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAVFL-KIEAEK 61
Query: 80 HRSVSQKYGVTGFPTIKIFTGSK 102
+++ + V P + G+K
Sbjct: 62 FSDIAESFDVNAVPLFVLIHGAK 84
Score = 35.5 bits (78), Expect = 0.014
Identities = 20/75 (26%), Positives = 36/75 (48%), Gaps = 2/75 (2%)
Query: 153 FKELVLDS-DDLWLVEFYAPWCGHCKNLEPHWAKAATELKGKVKLGALDATVHTTMASRY 211
F+E++ + + + L+ FYAPW CK + + + A + K V L ++A + +A +
Sbjct: 11 FQEILQNGKEQIILLNFYAPWAAPCKQMNQVFDQFAKDTKNAVFL-KIEAEKFSDIAESF 69
Query: 212 QVQGYPTIKLFPSGK 226
V P L K
Sbjct: 70 DVNAVPLFVLIHGAK 84
>SPBC20F10.07 |||GRAM domain protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 764
Score = 31.9 bits (69), Expect = 0.17
Identities = 16/37 (43%), Positives = 20/37 (54%)
Query: 9 TGSLALYDSSSDVIELTPSNFDKLVTNSDEIWIIEFF 45
TGS + SDV+ L+ S L+ SD WII FF
Sbjct: 428 TGSPLAHVLCSDVVNLSVSTVFNLLCGSDTTWIINFF 464
>SPBC211.01 |rsm10|SPBC23E6.11|mitochondrial ribosomal protein
subunit S10|Schizosaccharomyces pombe|chr 2|||Manual
Length = 224
Score = 30.7 bits (66), Expect = 0.40
Identities = 15/39 (38%), Positives = 22/39 (56%)
Query: 219 IKLFPSGKKSSDSAEDYNGGRTSSDIVTWALEKLAENVP 257
+K F S KS+D+ ++ G + DI+T EKL N P
Sbjct: 174 LKNFESQSKSTDNFKELLNGPSKKDIITQNAEKLLRNDP 212
>SPAC959.05c |||protein disulfide isomerase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 632
Score = 29.5 bits (63), Expect = 0.92
Identities = 13/60 (21%), Positives = 24/60 (40%)
Query: 49 CGHCKSLVPEYKKAARALKGIVKVGALDADEHRSVSQKYGVTGFPTIKIFTGSKHTPYQG 108
C C + R +K+ ++ DE + + + + FPT ++F G Y G
Sbjct: 209 CEDCFHWEAVWSSITRNTDERLKMAQVNCDEEKEMCNHFHIKKFPTFRVFQGFDSIQYNG 268
Score = 28.3 bits (60), Expect = 2.1
Identities = 14/65 (21%), Positives = 30/65 (46%), Gaps = 5/65 (7%)
Query: 173 CGHCKNLEPHWAKAATELKGKVKLGALDATVHTTMASRYQVQGYPTIKLFPSGKKSSDSA 232
C C + E W+ ++K+ ++ M + + ++ +PT ++F + DS
Sbjct: 209 CEDCFHWEAVWSSITRNTDERLKMAQVNCDEEKEMCNHFHIKKFPTFRVF----QGFDSI 264
Query: 233 EDYNG 237
+ YNG
Sbjct: 265 Q-YNG 268
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.317 0.134 0.416
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,726,400
Number of Sequences: 5004
Number of extensions: 67112
Number of successful extensions: 193
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 156
Number of HSP's gapped (non-prelim): 26
length of query: 419
length of database: 2,362,478
effective HSP length: 75
effective length of query: 344
effective length of database: 1,987,178
effective search space: 683589232
effective search space used: 683589232
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 55 (26.2 bits)
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