Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA002541-TA|BGIBMGA002541-PA|IPR001087|Lipolytic enzyme,
G-D-S-L, IPR008265|Lipolytic enzyme, G-D-S-L, active site,
IPR013830|Esterase, SGNH hydrolase-type
(381 letters)
Database: fruitfly
52,641 sequences; 24,830,863 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY069174-1|AAL39319.1| 424|Drosophila melanogaster GH22081p pro... 208 1e-53
AE014296-3278|AAF49069.1| 424|Drosophila melanogaster CG7365-PA... 208 1e-53
AE014134-966|AAF52285.1| 447|Drosophila melanogaster CG11029-PA... 183 3e-46
BT001688-1|AAN71443.1| 235|Drosophila melanogaster RE58281p pro... 36 0.12
BT003239-1|AAO24996.1| 312|Drosophila melanogaster LD48066p pro... 32 1.5
AY051411-1|AAK92835.1| 751|Drosophila melanogaster GH09153p pro... 32 1.5
AE014297-3430|AAF56217.1| 751|Drosophila melanogaster CG13604-P... 32 1.5
AE014134-688|AAN10366.1| 1154|Drosophila melanogaster CG3399-PC,... 31 3.5
BT024458-1|ABC86520.1| 1153|Drosophila melanogaster AT18380p pro... 29 8.1
BT021476-1|AAX33624.1| 1286|Drosophila melanogaster AT04667p pro... 29 8.1
>AY069174-1|AAL39319.1| 424|Drosophila melanogaster GH22081p
protein.
Length = 424
Score = 208 bits (507), Expect = 1e-53
Identities = 118/334 (35%), Positives = 168/334 (50%), Gaps = 9/334 (2%)
Query: 37 RSKERPTSVHKLRPGDIDVVAAIGDSLVAGSGALEEFALGAFVEYRGVSWCMGGDATWRE 96
RS ERPTSVH+LRPGDIDV+ A+GDSL AG+G L VE RGV W +GG WR+
Sbjct: 88 RSPERPTSVHRLRPGDIDVIGAMGDSLTAGNGIFATNLLHVTVENRGVVWSIGGQYDWRK 147
Query: 97 FLTLPNILKEFNPNLKGYSTGTGEWITKNSKLNVAFPVASDEDALKQAKIIVARMRASPD 156
+LTLPNILKEFNPNL GY+ G ++S+ +VA A D AK++V RM+ P
Sbjct: 148 YLTLPNILKEFNPNLYGYAIKDGISTDRSSRFDVAELAAMSRDMPHMAKVLVRRMQRDPR 207
Query: 157 IDIAQDWKMITMFIGANDICSAACINPVSWSPAA-HARKLANALDYLQEKLPR-TIXXXX 214
+++ DWK++T+FIG ND C+ C P H R + YL++ +PR +
Sbjct: 208 VNMTSDWKLVTLFIGNNDFCTDICYYPEPEKTVDWHERNMLKTYRYLRDNVPRLMLNIVP 267
Query: 215 XXXXXXXXXXXXXXMMCRLMHSLFCSC-FHRGGDELQDIVRMSRLYQKHEALLVDSGRYD 273
+C C C +G +L + + + + + + + ++
Sbjct: 268 APNLRFLTNLTGLPPICYSTLRFECPCLMGKGKGQLDYLEGIMKRWIAKDFEIANREEFN 327
Query: 274 AREDFTVVVQPFMRLFNAPFPPKQPLPLVIHQSYITHDCFHFSQKGHXXXXXXXXXXXXE 333
E FT+ VQPF + + P + + + DCFH SQ+GH E
Sbjct: 328 T-ETFTINVQPFSQFQDFP----RTRSGQTDTRFFSEDCFHLSQRGHAAAANSIWNNMLE 382
Query: 334 PVGNKSDNVPPVLMRSFRCPSRQAPFIFTARNTR 367
G+KS L +FRCPS PF+ T N+R
Sbjct: 383 LPGHKS-GFATHLFETFRCPSEMRPFLITRENSR 415
>AE014296-3278|AAF49069.1| 424|Drosophila melanogaster CG7365-PA
protein.
Length = 424
Score = 208 bits (507), Expect = 1e-53
Identities = 118/334 (35%), Positives = 168/334 (50%), Gaps = 9/334 (2%)
Query: 37 RSKERPTSVHKLRPGDIDVVAAIGDSLVAGSGALEEFALGAFVEYRGVSWCMGGDATWRE 96
RS ERPTSVH+LRPGDIDV+ A+GDSL AG+G L VE RGV W +GG WR+
Sbjct: 88 RSPERPTSVHRLRPGDIDVIGAMGDSLTAGNGIFATNLLHVTVENRGVVWSIGGQYDWRK 147
Query: 97 FLTLPNILKEFNPNLKGYSTGTGEWITKNSKLNVAFPVASDEDALKQAKIIVARMRASPD 156
+LTLPNILKEFNPNL GY+ G ++S+ +VA A D AK++V RM+ P
Sbjct: 148 YLTLPNILKEFNPNLYGYAIKDGISTDRSSRFDVAELAAMSRDMPHMAKVLVRRMQRDPR 207
Query: 157 IDIAQDWKMITMFIGANDICSAACINPVSWSPAA-HARKLANALDYLQEKLPR-TIXXXX 214
+++ DWK++T+FIG ND C+ C P H R + YL++ +PR +
Sbjct: 208 VNMTSDWKLVTLFIGNNDFCTDICYYPEPEKTVDWHERNMLKTYRYLRDNVPRLMLNIVP 267
Query: 215 XXXXXXXXXXXXXXMMCRLMHSLFCSC-FHRGGDELQDIVRMSRLYQKHEALLVDSGRYD 273
+C C C +G +L + + + + + + + ++
Sbjct: 268 APNLRFLTNLTGLPPICYSTLRFECPCLMGKGKGQLDYLEGIMKRWIAKDFEIANREEFN 327
Query: 274 AREDFTVVVQPFMRLFNAPFPPKQPLPLVIHQSYITHDCFHFSQKGHXXXXXXXXXXXXE 333
E FT+ VQPF + + P + + + DCFH SQ+GH E
Sbjct: 328 T-ETFTINVQPFSQFQDFP----RTRSGQTDTRFFSEDCFHLSQRGHAAAANSIWNNMLE 382
Query: 334 PVGNKSDNVPPVLMRSFRCPSRQAPFIFTARNTR 367
G+KS L +FRCPS PF+ T N+R
Sbjct: 383 LPGHKS-GFATHLFETFRCPSEMRPFLITRENSR 415
>AE014134-966|AAF52285.1| 447|Drosophila melanogaster CG11029-PA
protein.
Length = 447
Score = 183 bits (446), Expect = 3e-46
Identities = 112/357 (31%), Positives = 170/357 (47%), Gaps = 15/357 (4%)
Query: 12 RSHVRRQRAIPPSTPFPCEDALSWGRSKERPTSVHKLRPGDIDVVAAIGDSLVAGSGALE 71
R + Q + PF C + RS PTS+ LRPGDID++AA GDSL AG+G L
Sbjct: 93 RREGKLQAPVSKKVPFLCPTNNT--RSPSPPTSIEHLRPGDIDIIAAFGDSLSAGNGILS 150
Query: 72 EFALGAFVEYRGVSWCMGGDATWREFLTLPNILKEFNPNLKGYSTGTGEWIT-KNSKLNV 130
A+ E+RG+++ GG A WR F+TLPNILK FNP L G++ I ++S+LN+
Sbjct: 151 NNAIDMINEFRGLTFSGGGLANWRRFVTLPNILKIFNPKLYGFAVSNSLVINHRSSRLNI 210
Query: 131 AFPVASDEDALKQAKIIVARMRASPDIDIAQDWKMITMFIGANDICSAAC--INPVSWSP 188
A P+ D QA++++ +R +D+ + WK++T+++G NDICS C P S+
Sbjct: 211 AEPMIMSRDLPFQARVLIDLLRRDRHVDMKRHWKLLTVYVGNNDICSDLCHWDTPQSFLD 270
Query: 189 AAHARKLANALDYLQEKLPRTIXXXXX--XXXXXXXXXXXXXMMCRLMHSLFCSCFHRGG 246
HAR L A L++ +PR + + C ++H + C C
Sbjct: 271 -QHARDLRQAFRLLRDHVPRLLINLIVVPNIPLVLSTMTKVPLQCFVVHRVGCHCLINDR 329
Query: 247 DELQDIVRMSRLYQKHEALLVDSGRYD--AREDFTVVVQPFMRLFNAPFPPKQPLPLVIH 304
+ + + L ++ R R+DF +V P + AP P
Sbjct: 330 LNRTEFNERMDTLTRWQQLDMEIARLPEFRRQDFAIVAHPMLTKLTAPTLPDGS----TD 385
Query: 305 QSYITHDCFHFSQKGHXXXXXXXXXXXXEPVGNK-SDNVPPVLMRSFRCPSRQAPFI 360
+ +HDCFHFSQ+GH P K +V P L CP+ + P++
Sbjct: 386 WRFFSHDCFHFSQRGHAIISNLLWNSMLLPDDQKPRPSVVPELFERVVCPTAEQPYL 442
>BT001688-1|AAN71443.1| 235|Drosophila melanogaster RE58281p
protein.
Length = 235
Score = 35.5 bits (78), Expect = 0.12
Identities = 23/53 (43%), Positives = 28/53 (52%), Gaps = 5/53 (9%)
Query: 3 SWRVRYPVRR---SHVRRQRAIPPST-PFPCEDALSWG-RSKERPTSVHKLRP 50
SWR R P+RR S RRQR IPPST P W R+ R T++ + P
Sbjct: 141 SWRRRQPLRRRTRSSSRRQRRIPPSTWASPLPSPPFWACRASARTTTMPSISP 193
>BT003239-1|AAO24996.1| 312|Drosophila melanogaster LD48066p
protein.
Length = 312
Score = 31.9 bits (69), Expect = 1.5
Identities = 13/43 (30%), Positives = 23/43 (53%)
Query: 114 YSTGTGEWITKNSKLNVAFPVASDEDALKQAKIIVARMRASPD 156
Y +G +W+TKN F V D + ++ A + AR++ S +
Sbjct: 174 YPSGVPDWLTKNELTEAKFDVDLDYEPVQPASELTARLKESTE 216
>AY051411-1|AAK92835.1| 751|Drosophila melanogaster GH09153p
protein.
Length = 751
Score = 31.9 bits (69), Expect = 1.5
Identities = 13/43 (30%), Positives = 23/43 (53%)
Query: 114 YSTGTGEWITKNSKLNVAFPVASDEDALKQAKIIVARMRASPD 156
Y +G +W+TKN F V D + ++ A + AR++ S +
Sbjct: 613 YPSGVPDWLTKNELTEAKFDVDLDYEPVQPASELTARLKESTE 655
>AE014297-3430|AAF56217.1| 751|Drosophila melanogaster CG13604-PA
protein.
Length = 751
Score = 31.9 bits (69), Expect = 1.5
Identities = 13/43 (30%), Positives = 23/43 (53%)
Query: 114 YSTGTGEWITKNSKLNVAFPVASDEDALKQAKIIVARMRASPD 156
Y +G +W+TKN F V D + ++ A + AR++ S +
Sbjct: 613 YPSGVPDWLTKNELTEAKFDVDLDYEPVQPASELTARLKESTE 655
>AE014134-688|AAN10366.1| 1154|Drosophila melanogaster CG3399-PC,
isoform C protein.
Length = 1154
Score = 30.7 bits (66), Expect = 3.5
Identities = 29/104 (27%), Positives = 45/104 (43%), Gaps = 11/104 (10%)
Query: 31 DALSWGRSKERPTSV-HKLRPGDIDVVAAIGDSLVAGSGALEEFALGAFVEYRG-----V 84
D ++ G S+ R +V K PG +V +G + G G E + A E RG +
Sbjct: 62 DHINMGNSQNRAANVDEKSPPGGASLVR-MG---ITGGGGAGEVGV-ALSERRGSRVRVL 116
Query: 85 SWCMGGDATWREFLTLPNILKEFNPNLKGYSTGTGEWITKNSKL 128
S MG FL P + NL+G G+ +WI +++
Sbjct: 117 SRLMGQKRIREAFLHSPKLGSSSEVNLQGAGNGSSDWIAAETEV 160
>BT024458-1|ABC86520.1| 1153|Drosophila melanogaster AT18380p
protein.
Length = 1153
Score = 29.5 bits (63), Expect = 8.1
Identities = 28/102 (27%), Positives = 42/102 (41%), Gaps = 7/102 (6%)
Query: 31 DALSWGRSKERPTSV-HKLRPGDIDVVAAIGDSLVAGSGALEE-FALGA--FVEYRGVSW 86
D ++ G S+ R +V K PG +V + G GA E AL R +S
Sbjct: 62 DHINMGNSQNRAANVDEKSPPGGASLVRM---GITGGGGAGEVGVALSERRSSRVRVLSR 118
Query: 87 CMGGDATWREFLTLPNILKEFNPNLKGYSTGTGEWITKNSKL 128
MG FL P + NL+G G+ +WI +++
Sbjct: 119 LMGQKRIREAFLHSPKLGSSHEVNLEGAGNGSSDWIAAETEV 160
>BT021476-1|AAX33624.1| 1286|Drosophila melanogaster AT04667p
protein.
Length = 1286
Score = 29.5 bits (63), Expect = 8.1
Identities = 28/102 (27%), Positives = 42/102 (41%), Gaps = 7/102 (6%)
Query: 31 DALSWGRSKERPTSV-HKLRPGDIDVVAAIGDSLVAGSGALEE-FALGA--FVEYRGVSW 86
D ++ G S+ R +V K PG +V + G GA E AL R +S
Sbjct: 4 DHINMGNSQNRAANVDEKSPPGGASLVRM---GITGGGGAGEVGVALSERRSSRVRVLSR 60
Query: 87 CMGGDATWREFLTLPNILKEFNPNLKGYSTGTGEWITKNSKL 128
MG FL P + NL+G G+ +WI +++
Sbjct: 61 LMGQKRIREAFLHSPKLGSSHEVNLEGAGNGSSDWIAAETEV 102
Database: fruitfly
Posted date: Oct 5, 2007 11:13 AM
Number of letters in database: 24,830,863
Number of sequences in database: 52,641
Lambda K H
0.323 0.137 0.434
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 18,808,203
Number of Sequences: 52641
Number of extensions: 775475
Number of successful extensions: 1246
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 1230
Number of HSP's gapped (non-prelim): 13
length of query: 381
length of database: 24,830,863
effective HSP length: 86
effective length of query: 295
effective length of database: 20,303,737
effective search space: 5989602415
effective search space used: 5989602415
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (22.0 bits)
S2: 63 (29.5 bits)
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