Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA002504-TA|BGIBMGA002504-PA|IPR005225|Small GTP-binding
protein domain, IPR006687|GTP-binding protein SAR1, IPR006689|ARF/SAR
superfamily, IPR006688|ADP-ribosylation factor, IPR001806|Ras GTPase
(193 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC31F10.06c |sar1||ADP-ribosylation factor Sar1|Schizosaccharo... 278 3e-76
SPAC22F3.05c |alp41||ADP-ribosylation factor Alp41|Schizosacchar... 102 3e-23
SPBC1539.08 |||ADP-ribosylation factor, Arf family|Schizosacchar... 101 7e-23
SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomy... 97 2e-21
SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit... 40 2e-04
SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr 2||... 35 0.009
SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces ... 33 0.021
SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|ch... 32 0.063
SPAC20H4.11c |rho5||Rho family GTPase Rho5|Schizosaccharomyces p... 31 0.11
SPAC23H4.07c |srp102||signal recognition particle receptor beta ... 29 0.59
SPBC1271.12 |kes1||oxysterol binding protein |Schizosaccharomyce... 29 0.59
SPCC70.07c |tmp1|tmp|thymidylate kinase Tmp1|Schizosaccharomyces... 27 1.4
SPAC20G4.01 ||SPAC22F8.13|CCR4-Not complex subunit Caf16|Schizos... 27 1.8
SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|ch... 27 1.8
SPAC26F1.09 |gyp51||GTPase activating protein Gyp51 |Schizosacch... 27 2.4
SPAPB1A10.10c |ypt71||GTPase Ypt71|Schizosaccharomyces pombe|chr... 26 3.1
SPAC4C5.02c |ryh1|hos1|GTPase Ryh1|Schizosaccharomyces pombe|chr... 26 4.2
SPCC306.04c |set1||histone lysine methyltransferase Set1|Schizos... 26 4.2
SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit Mcm5|Schi... 25 7.3
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 25 9.6
SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomy... 25 9.6
>SPBC31F10.06c |sar1||ADP-ribosylation factor
Sar1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 190
Score = 278 bits (682), Expect = 3e-76
Identities = 137/193 (70%), Positives = 157/193 (81%), Gaps = 4/193 (2%)
Query: 1 MFILDWFTGVLGFLGLWKKSGKLLFLGLDNAGKTTLLHMLKDDRLAQHVPTLHPTSEELS 60
MFI++WF L LGL K K+LFLGLDNAGKTTLLHMLK+DRLA PTLHPTSEEL+
Sbjct: 1 MFIINWFYDALAMLGLVNKHAKMLFLGLDNAGKTTLLHMLKNDRLAVMQPTLHPTSEELA 60
Query: 61 IGSMRFTTFDLGGHQQARRVWRDYFPAVDAIVFLVDACDRPRLPESKAELDSLLTDETLS 120
IG++RFTTFDLGGHQQARR+WRDYFP V+ IV+LVD CD RL ESKAELD+LL E L+
Sbjct: 61 IGNVRFTTFDLGGHQQARRLWRDYFPEVNGIVYLVDCCDFERLSESKAELDALLAMEELA 120
Query: 121 NCPVLILGNKIDKPGAASEDELRHFFNLYQQTTGKGKVSRSELPG-RPLELFMCSVLKRQ 179
P LILGNKID PGA SEDEL+ LY QTTGKG VS+ +PG RP+E+FMCSV+ RQ
Sbjct: 121 RVPFLILGNKIDAPGAISEDELKAALGLY-QTTGKG-VSK-PVPGIRPIEVFMCSVVLRQ 177
Query: 180 GYGEGFRWLAQYI 192
GYGEGF+WLAQY+
Sbjct: 178 GYGEGFKWLAQYV 190
>SPAC22F3.05c |alp41||ADP-ribosylation factor
Alp41|Schizosaccharomyces pombe|chr 1|||Manual
Length = 186
Score = 102 bits (245), Expect = 3e-23
Identities = 54/151 (35%), Positives = 87/151 (57%), Gaps = 1/151 (0%)
Query: 16 LWKKSGKLLFLGLDNAGKTTLLHMLKDDRLAQHVPTLHPTSEELSIGSMRFTTFDLGGHQ 75
L ++ ++L LGLDNAGKTT+L L ++ + + PT L + +RFT +D+GG +
Sbjct: 12 LKEREVRVLLLGLDNAGKTTILKCLLNEDVNEVSPTFGFQIRTLEVEGLRFTIWDIGGQK 71
Query: 76 QARRVWRDYFPAVDAIVFLVDACDRPRLPESKAELDSLLTDETLSNCPVLILGNKIDKPG 135
R W++YF + +AI+++VD+ D RL E + L LL +E L +L+L NK D G
Sbjct: 72 TLRNFWKNYFESTEAIIWVVDSLDDLRLEECRNTLQELLVEEKLLFTSILVLANKSDVSG 131
Query: 136 AASEDELRHFFNLYQQTTGKGKV-SRSELPG 165
A S +E+ N+ + + ++ S S L G
Sbjct: 132 ALSSEEISKILNISKYKSSHWRIFSVSALTG 162
>SPBC1539.08 |||ADP-ribosylation factor, Arf
family|Schizosaccharomyces pombe|chr 2|||Manual
Length = 184
Score = 101 bits (242), Expect = 7e-23
Identities = 46/130 (35%), Positives = 79/130 (60%)
Query: 19 KSGKLLFLGLDNAGKTTLLHMLKDDRLAQHVPTLHPTSEELSIGSMRFTTFDLGGHQQAR 78
K ++L LGLD AGKTT+L+ LK ++ +PT+ E ++ +++F +D+GG + R
Sbjct: 20 KEMRILMLGLDAAGKTTILYKLKLNQSVVTIPTVGFNVETVTYKNIKFNVWDVGGQDKIR 79
Query: 79 RVWRDYFPAVDAIVFLVDACDRPRLPESKAELDSLLTDETLSNCPVLILGNKIDKPGAAS 138
+WR YF ++F+VD+ D R+ E++ EL +++D + +C +L+L NK D PGA S
Sbjct: 80 PLWRHYFTGTKGLIFVVDSADSNRISEARQELHRIISDREMRDCLLLVLANKQDLPGALS 139
Query: 139 EDELRHFFNL 148
++ L
Sbjct: 140 PAQITDVLQL 149
>SPBC4F6.18c |arf1||ADP-ribosylation factor Arf1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 180
Score = 96.7 bits (230), Expect = 2e-21
Identities = 46/132 (34%), Positives = 76/132 (57%)
Query: 18 KKSGKLLFLGLDNAGKTTLLHMLKDDRLAQHVPTLHPTSEELSIGSMRFTTFDLGGHQQA 77
K+ ++L +GLD AGKTT+L+ LK + +PT+ E + ++ FT +D+GG +
Sbjct: 15 KREMRILMVGLDAAGKTTILYKLKLGEIVTTIPTIGFNVETVEYRNISFTVWDVGGQDKI 74
Query: 78 RRVWRDYFPAVDAIVFLVDACDRPRLPESKAELDSLLTDETLSNCPVLILGNKIDKPGAA 137
R +WR YF I+F+VD+ DR R+ E+ EL +L ++ L + +L+ NK D P A
Sbjct: 75 RPLWRHYFQNTQGIIFVVDSNDRERISEAHEELQRMLNEDELRDALLLVFANKQDLPNAM 134
Query: 138 SEDELRHFFNLY 149
+ E+ L+
Sbjct: 135 NAAEITDKLGLH 146
>SPAC23H3.13c |gpa2|git8|heterotrimeric G protein alpha-2 subunit
Gpa2 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 354
Score = 39.9 bits (89), Expect = 2e-04
Identities = 35/131 (26%), Positives = 57/131 (43%), Gaps = 18/131 (13%)
Query: 42 DDRLAQHVPTLHPTSEELSIGSMRFTTFDLGGHQQARRVWRDYFPAVDAIVFLVD----- 96
DD L TL + ++ ++ FD+GG + RR W F V++I+F V
Sbjct: 170 DDILRSRNSTLGISEISFTLDHLQIRMFDVGGQRTERRKWIYCFENVNSIIFCVSLNDYD 229
Query: 97 -------ACDRPRLPESKAELDSLLTDETLSNCPVLILGNKIDKPGAASEDELRH--FFN 147
A +R RL ES + DS++ + + +++ NK D +L H F +
Sbjct: 230 KKLYERAAPERNRLVESISLFDSIINSQWFMHSSIILFLNKFD----LFRKKLEHVPFQD 285
Query: 148 LYQQTTGKGKV 158
+ Q GK V
Sbjct: 286 YFPQYEGKNSV 296
>SPBC1703.10 |ypt1||GTPase Ypt1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 203
Score = 34.7 bits (76), Expect = 0.009
Identities = 27/116 (23%), Positives = 49/116 (42%), Gaps = 6/116 (5%)
Query: 22 KLLFLGLDNAGKTTLLHMLKDDRLAQ-HVPTLHPTSE----ELSIGSMRFTTFDLGGHQQ 76
KLL +G GK+ LL DD + ++ T+ + EL +++ +D G ++
Sbjct: 10 KLLLIGDSGVGKSCLLLRFADDTYTESYISTIGVDFKIRTFELEGKTVKLQIWDTAGQER 69
Query: 77 ARRVWRDYFPAVDAIVFLVDACDRPRLPESKAELDSLLTDETLSNCPVLILGNKID 132
R + Y+ I+ + D D+ K L + + L++GNK D
Sbjct: 70 FRTITSSYYRGAHGIIIVYDVTDQDSFNNVKQWLQE-IDRYAVEGVNRLLVGNKSD 124
>SPBC24C6.06 |gpa1||G-protein alpha subunit |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 407
Score = 33.5 bits (73), Expect = 0.021
Identities = 26/100 (26%), Positives = 42/100 (42%), Gaps = 10/100 (10%)
Query: 43 DRLAQHVPTLHPTSEELSIGSMRFTTFDLGGHQQARRVWRDYFPAVDAIVFLV-----DA 97
D L + T + E + + FD+GG + RR W F V A++FLV D
Sbjct: 213 DILHCRIKTTGISEETFLLNRHHYRFFDVGGQRSERRKWIHCFENVTALLFLVSLAGYDQ 272
Query: 98 C-----DRPRLPESKAELDSLLTDETLSNCPVLILGNKID 132
C ++ E+ DS+ S +++ NK+D
Sbjct: 273 CLVEDNSGNQMQEALLLWDSICNSSWFSESAMILFLNKLD 312
>SPBC1289.03c |spi1||Ran GTPase Spi1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 216
Score = 31.9 bits (69), Expect = 0.063
Identities = 26/116 (22%), Positives = 47/116 (40%), Gaps = 7/116 (6%)
Query: 22 KLLFLGLDNAGKTTLLHM-LKDDRLAQHVPTL----HPTSEELSIGSMRFTTFDLGGHQQ 76
KL+ +G GKTT + L + +++ TL HP + G + F +D G ++
Sbjct: 11 KLVLVGDGGTGKTTFVKRHLTGEFEKKYIATLGVEVHPLHFHTNFGEICFNVWDTAGQEK 70
Query: 77 ARRVWRDYFPAVDAIVFLVDACDRPRLPESKAELDSLLTDETLSNCPVLILGNKID 132
+ Y+ + + D R L+ N P+++ GNK+D
Sbjct: 71 LGGLRDGYYIQGQCGIIMFDVTSRITYKNVPHWWRDLV--RVCENIPIVLCGNKVD 124
>SPAC20H4.11c |rho5||Rho family GTPase Rho5|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 200
Score = 31.1 bits (67), Expect = 0.11
Identities = 32/118 (27%), Positives = 52/118 (44%), Gaps = 11/118 (9%)
Query: 22 KLLFLGLDNAGKTTLLHMLKDDRLAQ-HVPTL---HPTSEELSIGSMRFTTFDLGGHQQA 77
KL+ +G GKT LL + + +VPT+ + E+ + +D G +
Sbjct: 8 KLVIVGDGACGKTCLLIVFSKGTFPEVYVPTVFENYVADVEVDGRHIELALWDTAGQEDY 67
Query: 78 RRVWRDYFPAVDAIVFLVDACDRPRLPESKAELDSLLTDETL---SNCPVLILGNKID 132
R+ +P D+ V L+ C PES + E L SN P+L++G K+D
Sbjct: 68 DRLRPLSYP--DSHVVLI--CFSVDAPESLDNVQEKWISEVLHFCSNLPILLVGCKVD 121
>SPAC23H4.07c |srp102||signal recognition particle receptor beta
subunit Srp102 |Schizosaccharomyces pombe|chr 1|||Manual
Length = 227
Score = 28.7 bits (61), Expect = 0.59
Identities = 23/83 (27%), Positives = 38/83 (45%), Gaps = 6/83 (7%)
Query: 18 KKSGKLLFLGLDNAGKTTLLHMLKDDRLAQHVPTLHPTSEELSIGSMRFTTFDLGGHQQA 77
KK + +G ++GKT+L L VP++ P G+ DL GH +A
Sbjct: 35 KKLPAVFLIGPSDSGKTSLFCELIYKEKKTTVPSIEPNEAVWKYGAW---LVDLPGHPRA 91
Query: 78 RRVWRDYFPA---VDAIVFLVDA 97
+R F V A+VF++++
Sbjct: 92 KRWITTKFSGNYNVKAVVFVLNS 114
>SPBC1271.12 |kes1||oxysterol binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 388
Score = 28.7 bits (61), Expect = 0.59
Identities = 11/38 (28%), Positives = 24/38 (63%)
Query: 73 GHQQARRVWRDYFPAVDAIVFLVDACDRPRLPESKAEL 110
G ++RRVW+++ A+DA + + ++ ++ E + EL
Sbjct: 306 GEYESRRVWKNFAVALDAGDYAAASQEKSKIEEGQREL 343
>SPCC70.07c |tmp1|tmp|thymidylate kinase Tmp1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 210
Score = 27.5 bits (58), Expect = 1.4
Identities = 12/29 (41%), Positives = 20/29 (68%), Gaps = 1/29 (3%)
Query: 21 GKLLFL-GLDNAGKTTLLHMLKDDRLAQH 48
G+L+ + GLD +GK+T +L D ++QH
Sbjct: 7 GRLIVIEGLDRSGKSTQCQLLVDKLISQH 35
>SPAC20G4.01 ||SPAC22F8.13|CCR4-Not complex subunit
Caf16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 280
Score = 27.1 bits (57), Expect = 1.8
Identities = 12/29 (41%), Positives = 18/29 (62%)
Query: 19 KSGKLLFLGLDNAGKTTLLHMLKDDRLAQ 47
K + L +G + AGK+TLL +L LA+
Sbjct: 29 KGSRTLLVGANGAGKSTLLKLLSGKSLAK 57
>SPAC17H9.09c |ras1|ste5|GTPase Ras1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 219
Score = 27.1 bits (57), Expect = 1.8
Identities = 27/151 (17%), Positives = 58/151 (38%), Gaps = 10/151 (6%)
Query: 22 KLLFLGLDNAGKTTL-LHMLKDDRLAQHVPTLHPTSE---ELSIGSMRFTTFDLGGHQQA 77
KL+ +G GK+ L + +++ + ++ PT+ + E+ D G ++
Sbjct: 10 KLVVVGDGGVGKSALTIQLIQSHFVDEYDPTIEDSYRKKCEIDGEGALLDVLDTAGQEEY 69
Query: 78 RRVWRDYFPAVDAIVFLVDACDRPRLPESKAELDSLLTDETLSNCPVLILGNKIDKPG-- 135
+ Y + + + + R E +L + PV+++ NK D
Sbjct: 70 SAMREQYMRTGEGFLLVYNITSRSSFDEISTFYQQILRVKDKDTFPVVLVANKCDLEAER 129
Query: 136 ----AASEDELRHFFNLYQQTTGKGKVSRSE 162
A E + LY +T+ K +++ E
Sbjct: 130 VVSRAEGEQLAKSMHCLYVETSAKLRLNVEE 160
>SPAC26F1.09 |gyp51||GTPase activating protein Gyp51
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1031
Score = 26.6 bits (56), Expect = 2.4
Identities = 14/48 (29%), Positives = 22/48 (45%)
Query: 142 LRHFFNLYQQTTGKGKVSRSELPGRPLELFMCSVLKRQGYGEGFRWLA 189
L HFF+ QQ ++ S + + VL + GY +G W+A
Sbjct: 662 LSHFFSNRQQLEPTDNIAESTANLHRVLRSLAIVLPQVGYTQGMSWIA 709
>SPAPB1A10.10c |ypt71||GTPase Ypt71|Schizosaccharomyces pombe|chr
1|||Manual
Length = 208
Score = 26.2 bits (55), Expect = 3.1
Identities = 22/119 (18%), Positives = 52/119 (43%), Gaps = 7/119 (5%)
Query: 22 KLLFLGLDNAGKTTLLHMLKDDRLA-QHVPTLHPT--SEELSIGSMRFTT--FDLGGHQQ 76
K++ LG GKT L++ + + + ++ T+ ++++ + T +D G ++
Sbjct: 10 KVVILGDSGVGKTCLMNQFVNQKFSREYKATIGADFLTKDVVVDDKLVTLQLWDTAGQER 69
Query: 77 ARRVWRDYFPAVDAIVFLVDACDRPRLPESKAELDSLLTDETLSNC--PVLILGNKIDK 133
+ + ++ D V + + + + L + C P +I+GN+IDK
Sbjct: 70 FQSLGMAFYRGADCCVIVYNVNNSKSFDSVENWRQEFLYQTSQDECAFPFIIVGNQIDK 128
>SPAC4C5.02c |ryh1|hos1|GTPase Ryh1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 201
Score = 25.8 bits (54), Expect = 4.2
Identities = 24/116 (20%), Positives = 52/116 (44%), Gaps = 6/116 (5%)
Query: 22 KLLFLGLDNAGKTTLL-HMLKD--DRLAQHVPTLHPTSEELSI--GSMRFTTFDLGGHQQ 76
KL+FLG + GKT+L+ + D D Q + S+ + + ++R +D G ++
Sbjct: 13 KLVFLGEQSVGKTSLITRFMYDQFDNTYQATIGIDFLSKTMYLEDRTVRLQLWDTAGQER 72
Query: 77 ARRVWRDYFPAVDAIVFLVDACDRPRLPESKAELDSLLTDETLSNCPVLILGNKID 132
R + Y + + D + ++ ++ + E + ++++GNK D
Sbjct: 73 FRSLIPSYIRDSSVAIIVYDITNHNSFVNTEKWIEDVRA-ERGDDVIIVLVGNKTD 127
>SPCC306.04c |set1||histone lysine methyltransferase
Set1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 920
Score = 25.8 bits (54), Expect = 4.2
Identities = 14/36 (38%), Positives = 20/36 (55%)
Query: 107 KAELDSLLTDETLSNCPVLILGNKIDKPGAASEDEL 142
K E+D + ET S+ +L I K G+A +DEL
Sbjct: 556 KMEVDYTSSSETESDASILSPSAAIPKSGSAIKDEL 591
>SPAC1B2.05 |mcm5|nda4, SPAC3F10.01|MCM complex subunit
Mcm5|Schizosaccharomyces pombe|chr 1|||Manual
Length = 720
Score = 25.0 bits (52), Expect = 7.3
Identities = 14/33 (42%), Positives = 19/33 (57%), Gaps = 4/33 (12%)
Query: 41 KDDRLAQHVPTLH----PTSEELSIGSMRFTTF 69
KD +A+HV LH +SE L+IG + F F
Sbjct: 520 KDRNIARHVINLHTNLQESSETLAIGEIPFDKF 552
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 24.6 bits (51), Expect = 9.6
Identities = 12/44 (27%), Positives = 21/44 (47%)
Query: 138 SEDELRHFFNLYQQTTGKGKVSRSELPGRPLELFMCSVLKRQGY 181
S D + H +Y+ + R +L GR L ++ +L +GY
Sbjct: 155 SGDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGY 198
>SPAC26F1.07 |||2-methylbutyraldehyde reductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 321
Score = 24.6 bits (51), Expect = 9.6
Identities = 14/38 (36%), Positives = 21/38 (55%), Gaps = 2/38 (5%)
Query: 19 KSGKLLFLGLDNAGKTTLLHMLKDDRL--AQHVPTLHP 54
++GK+ +GL N T L +LK ++ A H LHP
Sbjct: 157 ETGKVRHIGLSNFNDTNLERILKVAKVKPAVHQMELHP 194
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.323 0.141 0.440
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 926,108
Number of Sequences: 5004
Number of extensions: 37909
Number of successful extensions: 113
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 98
Number of HSP's gapped (non-prelim): 23
length of query: 193
length of database: 2,362,478
effective HSP length: 69
effective length of query: 124
effective length of database: 2,017,202
effective search space: 250133048
effective search space used: 250133048
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 51 (24.6 bits)
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