GeneModel BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA002478-TA|BGIBMGA002478-PA|IPR000649|Initiation factor
2B related, IPR011559|Initiation factor 2B alpha/beta/delta
         (352 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC23E6.10c |||methylthioribose-1-phosphate isomerase |Schizosa...   307   2e-84
SPCC11E10.07c |||translation initiation factor eIF2B alpha subun...    76   9e-15
SPAC343.14c |||translation initiation factor eIF2B|Schizosacchar...    63   7e-11
SPAC21E11.06 |tif224||translation initiation factor eIF2B delta ...    36   0.009
SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit Psm3|Sch...    27   4.0  
SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr 2|||M...    26   7.0  

>SPBC23E6.10c |||methylthioribose-1-phosphate isomerase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 359

 Score =  307 bits (753), Expect = 2e-84
 Identities = 173/359 (48%), Positives = 226/359 (62%), Gaps = 29/359 (8%)

Query: 1   MSLESIKYTRGSXXXXXXXXXXXQTRYIKVRGVEDGWKVINKMQVRGAPAIAIVGCLSLA 60
           MSL++I Y               + +YI V  VED + VI KMQVRGAPAIAIV  LS+A
Sbjct: 1   MSLQAIIYNEDKLLVLDQLLLPYERKYISVSNVEDAFAVIKKMQVRGAPAIAIVAALSVA 60

Query: 61  VELSPDNESSKKNMRQEIEGKLNYLVSARPTAVNIKLAADELINLANTLCADDSISAEIF 120
           V L   +E +  + ++ +     +L  +RPTAVN+   A  +  +A           +  
Sbjct: 61  VGLKHLSEDA--DAKEYVINSFEHLKQSRPTAVNLFETAKFMQGIALQ-------EGKNC 111

Query: 121 KERFIGSIEDMLTKDIHDNKAIGSFGCEAILKNIDGDSPVRVLTHCNTGSLATAGYGTAL 180
           + + I   E ML KD+ DN  IG+ G + +L++      + VLTHCNTGSLAT+GYGTAL
Sbjct: 112 RNKIIQEAERMLVKDLEDNHNIGTAGRKFLLQHYKDKDKLTVLTHCNTGSLATSGYGTAL 171

Query: 181 GVIRSLHATKRLEHVFCTETRPYNQGARLTAYELVHEKIPSTLIVDRMVSALMHTRKIHA 240
           G+IRSLH +  LEH +CTETRPYNQG+RLTA+ELVH+KIP+TL+ D  V+++MH  KI A
Sbjct: 172 GIIRSLHESGNLEHAYCTETRPYNQGSRLTAFELVHDKIPATLVTDSTVASIMH--KIDA 229

Query: 241 VIVGADRVAANGDTANKIGTYQIAIVAKYHDVPFYVAAPLTSIDMSLPYGEKINIEERPD 300
           ++VGADRV  NGDTANKIGTY +AI+AK+   PF VAAP +SID SL  G +I IE+RP 
Sbjct: 230 IVVGADRVTRNGDTANKIGTYNLAILAKHFGKPFIVAAPFSSIDTSLASGSQITIEQRPP 289

Query: 301 REMTHI-------GEHR-----------IAAPGINCWNPSFDVTPASLIAGIITEKGVF 341
            EMT I          R           IAAPGI+ ++P+FDVTPA+LI  I TEKG +
Sbjct: 290 IEMTTITGPIITDSNSRNLEDPKRVRISIAAPGIDVYSPAFDVTPANLITAIATEKGFY 348


>SPCC11E10.07c |||translation initiation factor eIF2B alpha
           subunit|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 341

 Score = 75.8 bits (178), Expect = 9e-15
 Identities = 57/192 (29%), Positives = 90/192 (46%), Gaps = 27/192 (14%)

Query: 171 LATAGYGTALGVIRSLHATKRLEH-VFCTETRPYNQGARLTAYELVHEKIPSTLIVDRMV 229
           + T G+   +  +    A + +   VF TE+RP   G  +T   L +  IP+ +++D  V
Sbjct: 139 ILTHGFSRGVAAVLLAAAKRHVRFKVFVTESRPSGSGCLMTR-TLKNACIPTCMVLDSAV 197

Query: 230 SALMHTRKIHAVIVGADRVAANGDTANKIGTYQIAIVAKYHDVPFYVAA---------PL 280
           S  M+  ++  V+VGA+ V  NG   N+IGT+Q+A+ AK+   PFY  A         PL
Sbjct: 198 SFTMN--RVDLVLVGAEGVVENGGLINQIGTFQLAVFAKHAHKPFYAVAESHKFVRMFPL 255

Query: 281 TSIDMSLPYGEKINIEERPDREMTHIGEHRIAAPGINCW------------NPSFDVTPA 328
           +  D  +P+   I   + P  E  H     I  P                 NP+ DVTP 
Sbjct: 256 SQYD--IPFSRPILEFDDPSPETVHPEPEPIPTPSDAIHNELIMNEEQIRNNPTLDVTPP 313

Query: 329 SLIAGIITEKGV 340
             ++G+IT+ G+
Sbjct: 314 EFVSGLITDLGI 325


>SPAC343.14c |||translation initiation factor
           eIF2B|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 393

 Score = 62.9 bits (146), Expect = 7e-11
 Identities = 54/199 (27%), Positives = 83/199 (41%), Gaps = 7/199 (3%)

Query: 151 LKNIDGDSPVRVLTHCNTGSLA-TAGYGTALGVIRSLHATKRLEHVFCTETRPYNQ-GAR 208
           L  I+ D  V+ + H ++  +  T G    +       A KR   V   E  P NQ G+ 
Sbjct: 184 LDKINTDIEVQSMDHLHSNEIILTQGCSKTVEAFLRFAAKKRKFSVIVAEGFPNNQKGSH 243

Query: 209 LTAYELVHEKIPSTLIVDRMVSALMHTRKIHAVIVGADRVAANGDTANKIGTYQIAIVAK 268
             A  L    I +T+I D  + A+M   +++ VI+G   +  NG      G   +A  A+
Sbjct: 244 AMAKRLAQAGIDTTVISDATIFAIMS--RVNKVILGTHAILGNGGLVTYSGAQLVAQAAR 301

Query: 269 YHDVPFYVAAPLTSIDMSLPYG-EKINIEERPDREMTHIGEHRIAAPGINCWNPSFDVTP 327
           +H  P  V + +  +    PY  E I     PD+ M+      I+   I   NP +D  P
Sbjct: 302 HHATPVVVCSGIYKLSPVYPYDLESIIQLSSPDKIMSFNEGDLISRAEI--LNPYYDYIP 359

Query: 328 ASLIAGIITEKGVFAPDNL 346
             L+   IT  G + P  L
Sbjct: 360 PDLVDLFITNLGGYPPSYL 378


>SPAC21E11.06 |tif224||translation initiation factor eIF2B delta
           subunit|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 467

 Score = 35.9 bits (79), Expect = 0.009
 Identities = 62/300 (20%), Positives = 123/300 (41%), Gaps = 35/300 (11%)

Query: 64  SPDNESSKKNMRQEIEGKLNYLVSARPTAVNIKLAADELINLANTLCAD--DSISAEIFK 121
           +P   +  +++   I  ++ YLVS RP ++++  A   L    + L  D  D    E+  
Sbjct: 180 TPYGTTLSRHLTTHINSQIAYLVSTRPLSISMGNAIRFLKLEISVLDIDLTDDEGKELLL 239

Query: 122 ERFIGSIEDMLTKDIHDNKAIGSFGCEAILKNIDGDSPVRVLTHCNTGSLATAGYGTALG 181
           E+    I D +   I   + I     E I    DGD    +LT+ ++ ++          
Sbjct: 240 EKIDSYIRDRI---IIAGQVIVQAATEKIQ---DGDV---ILTYLHSSTVNDV------- 283

Query: 182 VIRSLHATKRLEHVFCTETRPYNQGARLTAYELVHEKIPSTLIVDRMVSALMH-TRKIHA 240
           +I + +  K+   V   ++RP  +G R+    L    I  T +   M+SAL +  +++  
Sbjct: 284 LIHAKNVGKKF-RVVVVDSRPEFEG-RVCLKLLTEHGIECTYV---MISALSYIMQEVTK 338

Query: 241 VIVGADRVAANGDTANKIGTYQIAIVAKYHDVPFYVAAPLTSIDMSLPYGEKINIEERPD 300
           + +G   + +NG   ++ GT  I+++    +VP             +     +  E  P 
Sbjct: 339 IFLGGHAMLSNGALYSRAGTSLISLLGHESNVPVIACCESYKFTERIQLDSLVYNELAPG 398

Query: 301 REMTHIG-EHRIAAPGINC-WNP---------SFDVTPASLIAGIITEKGVFAPDNLKSV 349
            ++ ++G +     PG+   W            +DVTP  LI   + E G+    ++ ++
Sbjct: 399 DQLVNMGVDDFEEKPGVLANWKSVKNLKLLSLKYDVTPPRLITVCVCEMGLLPSTSVPAI 458


>SPAC10F6.09c |psm3|smc3|mitotic cohesin complex subunit
           Psm3|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1194

 Score = 27.1 bits (57), Expect = 4.0
 Identities = 12/27 (44%), Positives = 18/27 (66%)

Query: 57  LSLAVELSPDNESSKKNMRQEIEGKLN 83
           LS  +E S  +ESSK N+  E+E K++
Sbjct: 312 LSRQIEFSKKDESSKLNILSELESKIS 338


>SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 685

 Score = 26.2 bits (55), Expect = 7.0
 Identities = 10/27 (37%), Positives = 13/27 (48%)

Query: 296 EERPDREMTHIGEHRIAAPGINCWNPS 322
           E+ P  +      H  A P INCW P+
Sbjct: 479 EDGPTHQPIETFAHFRAMPNINCWRPA 505


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.385 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,460,391
Number of Sequences: 5004
Number of extensions: 57974
Number of successful extensions: 117
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 105
Number of HSP's gapped (non-prelim): 7
length of query: 352
length of database: 2,362,478
effective HSP length: 73
effective length of query: 279
effective length of database: 1,997,186
effective search space: 557214894
effective search space used: 557214894
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 54 (25.8 bits)