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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA002431-TA|BGIBMGA002431-PA|IPR007371|Thiamin
pyrophosphokinase, catalytic region, IPR007373|Thiamin
pyrophosphokinase, vitamin B1-binding region
         (128 letters)

Database: nematostella 
           59,808 sequences; 16,821,457 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SB_18293| Best HMM Match : TPK_B1_binding (HMM E-Value=1e-24)          51   3e-07
SB_19314| Best HMM Match : Rho_N (HMM E-Value=5.2e-05)                 31   0.30 
SB_19492| Best HMM Match : Ank (HMM E-Value=1.5e-05)                   28   2.8  
SB_31804| Best HMM Match : No HMM Matches (HMM E-Value=.)              28   2.8  
SB_54591| Best HMM Match : No HMM Matches (HMM E-Value=.)              27   3.6  
SB_49608| Best HMM Match : No HMM Matches (HMM E-Value=.)              26   8.4  
SB_41135| Best HMM Match : Ribonuc_red_lgN (HMM E-Value=0.78)          26   8.4  
SB_25961| Best HMM Match : No HMM Matches (HMM E-Value=.)              26   8.4  

>SB_18293| Best HMM Match : TPK_B1_binding (HMM E-Value=1e-24)
          Length = 262

 Score = 51.2 bits (117), Expect = 3e-07
 Identities = 26/81 (32%), Positives = 42/81 (51%), Gaps = 3/81 (3%)

Query: 8  RYAVLILNRPISQEEAFMKNFWEKATLRITVDGGALRWDQFLTRLPEEISNKMK---IPD 64
          ++A+LILN P  + + F+   W KA   + VDG A           + + ++ +   +PD
Sbjct: 14 KFALLILNCPFGKVKKFLPILWRKAVFTVCVDGAANHLHTHFFSDIDSVFSEYELDFVPD 73

Query: 65 LITGDFDSITEETLQKYKKKG 85
           ITGDFDSI +  L+    +G
Sbjct: 74 FITGDFDSINKHVLEDLSARG 94



 Score = 41.9 bits (94), Expect = 2e-04
 Identities = 19/51 (37%), Positives = 31/51 (60%)

Query: 74  TEETLQKYKKKGYNQSLKFGELVSTSNAFDGSDTVKIKCSHTILWSMKVPS 124
           T+ T    K    + +L+FG L+STSN  DG+  V I+ + T+LW++ + S
Sbjct: 201 TDVTTSGLKWNLEHSTLEFGSLISTSNMLDGTGLVNIETNETLLWTIGIKS 251


>SB_19314| Best HMM Match : Rho_N (HMM E-Value=5.2e-05)
          Length = 708

 Score = 31.1 bits (67), Expect = 0.30
 Identities = 16/47 (34%), Positives = 25/47 (53%), Gaps = 2/47 (4%)

Query: 29  WEKATLRITVDGGALRWDQFLTRLPEEISNKMKIPDLITGDFDSITE 75
           W K   ++ V G   RW   +T   +  S KM++P ++  DF+S TE
Sbjct: 467 WIKDVSKL-VSGVKWRWRN-ITSTAQRTSRKMRVPFVVYADFESFTE 511


>SB_19492| Best HMM Match : Ank (HMM E-Value=1.5e-05)
          Length = 559

 Score = 27.9 bits (59), Expect = 2.8
 Identities = 20/74 (27%), Positives = 33/74 (44%), Gaps = 4/74 (5%)

Query: 49  LTRLPEEISNKMKIPDLITGDFDSITEETLQKYK---KKGYNQSLKFGELV-STSNAFDG 104
           + RL    ++K KIPD+ T    ++ E+  + YK   K   +  + F EL+    N  D 
Sbjct: 396 IKRLINITTDKQKIPDMCTEQTRTLIEDVTRDYKIENKNFQDYYMSFLELLEEAKNLADE 455

Query: 105 SDTVKIKCSHTILW 118
            D   +  +   LW
Sbjct: 456 EDISALNKTMLCLW 469


>SB_31804| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 2047

 Score = 27.9 bits (59), Expect = 2.8
 Identities = 20/74 (27%), Positives = 33/74 (44%), Gaps = 4/74 (5%)

Query: 49   LTRLPEEISNKMKIPDLITGDFDSITEETLQKYK---KKGYNQSLKFGELV-STSNAFDG 104
            + RL    ++K KIPD+ T    ++ E+  + YK   K   +  + F EL+    N  D 
Sbjct: 1844 IKRLINITTDKQKIPDMCTEQTRTLIEDVTRDYKIENKNFQDYYMSFLELLEEAKNLADE 1903

Query: 105  SDTVKIKCSHTILW 118
             D   +  +   LW
Sbjct: 1904 EDISALNKTMLCLW 1917


>SB_54591| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 173

 Score = 27.5 bits (58), Expect = 3.6
 Identities = 14/53 (26%), Positives = 22/53 (41%)

Query: 49  LTRLPEEISNKMKIPDLITGDFDSITEETLQKYKKKGYNQSLKFGELVSTSNA 101
           +  +PE I+ ++         FD+   +     +K GYN  LKF       NA
Sbjct: 104 IRNIPESINRRLSKISYDEETFDNAAPQYQNTLRKSGYNYMLKFQPRAHRPNA 156


>SB_49608| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 197

 Score = 26.2 bits (55), Expect = 8.4
 Identities = 11/23 (47%), Positives = 15/23 (65%)

Query: 20 QEEAFMKNFWEKATLRITVDGGA 42
          Q  A+   F +  T+R+TVDGGA
Sbjct: 71 QSPAYFDVFADHLTVRVTVDGGA 93


>SB_41135| Best HMM Match : Ribonuc_red_lgN (HMM E-Value=0.78)
          Length = 230

 Score = 26.2 bits (55), Expect = 8.4
 Identities = 18/57 (31%), Positives = 22/57 (38%)

Query: 5   PSLRYAVLILNRPISQEEAFMKNFWEKATLRITVDGGALRWDQFLTRLPEEISNKMK 61
           P  RY + I   P  Q E      WE  T     + GAL +D    +  E   NK K
Sbjct: 170 PGKRYRLAIGGSPFIQNERPKWQCWEAMTDEEYQEAGALAFDISNLKYKEPPPNKKK 226


>SB_25961| Best HMM Match : No HMM Matches (HMM E-Value=.)
          Length = 180

 Score = 26.2 bits (55), Expect = 8.4
 Identities = 12/25 (48%), Positives = 16/25 (64%)

Query: 51 RLPEEISNKMKIPDLITGDFDSITE 75
          RL E ++ K KI +L   DF+ ITE
Sbjct: 51 RLEEFLNEKRKIGELSNDDFEKITE 75


  Database: nematostella
    Posted date:  Oct 22, 2007  1:22 PM
  Number of letters in database: 16,821,457
  Number of sequences in database:  59,808
  
Lambda     K      H
   0.316    0.133    0.390 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,239,771
Number of Sequences: 59808
Number of extensions: 157670
Number of successful extensions: 393
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 385
Number of HSP's gapped (non-prelim): 9
length of query: 128
length of database: 16,821,457
effective HSP length: 74
effective length of query: 54
effective length of database: 12,395,665
effective search space: 669365910
effective search space used: 669365910
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 55 (26.2 bits)

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