GeneModel BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA002346-TA|BGIBMGA002346-PA|IPR006140|D-isomer specific
2-hydroxyacid dehydrogenase, NAD-binding, IPR006139|D-isomer specific
2-hydroxyacid dehydrogenase, catalytic region
         (487 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase |Schi...   120   5e-28
SPAC186.07c |||hydroxyacid dehydrogenase |Schizosaccharomyces po...   113   7e-26
SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces po...   109   8e-25
SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces po...   105   1e-23
SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase |Schizosac...    96   8e-21
SPBC56F2.12 |ilv5||acetohydroxyacid reductoisomerase|Schizosacch...    28   3.4  

>SPCC364.07 ||SPCC4G3.01|D-3 phosphoglycerate dehydrogenase
           |Schizosaccharomyces pombe|chr 3|||Manual
          Length = 466

 Score =  120 bits (289), Expect = 5e-28
 Identities = 76/218 (34%), Positives = 119/218 (54%), Gaps = 14/218 (6%)

Query: 82  LTKEDLEKFKALRIIVRIGSGVDNIDVKAAGELGIAVCNVPGYGVEEVADTTMCLILNLY 141
           LT+  LE   +L +I     G + +D+  A E GIAV N P      VA+  +  I++L 
Sbjct: 109 LTRRVLEAADSLIVIGCFCIGTNQVDLDFAAERGIAVFNSPYANSRSVAELVIGYIISLA 168

Query: 142 RRTYWLANMVREGKKFTGPEQVREASAGCARIRGDTLGIVGLGRIGSAVALRAKAFGFNV 201
           R+    +  +  G       +  + S+GC  IRG TLGI+G G IGS +++ A+A G +V
Sbjct: 169 RQVGDRSLELHRG-------EWNKVSSGCWEIRGKTLGIIGYGHIGSQLSVLAEAMGLHV 221

Query: 202 IFYD--PYLPDGIEKSLGLTRVYTLQDLLFQSDCVSLHCSLNEHNHHLINEFTIKQMRPG 259
           ++YD  P +P G  K L      +L +LL ++D VSLH   +    ++I+      M+ G
Sbjct: 222 VYYDILPIMPLGSAKQLS-----SLPELLHRADFVSLHVPASPETKNMISSKEFAAMKEG 276

Query: 260 AFLVNTARGGLVDDEGLAAALKQGRIRAAALDVHENEP 297
           ++L+N +RG +VD   L  A K G+I  AA+DV+ +EP
Sbjct: 277 SYLINASRGTVVDIPALVDASKSGKIAGAAIDVYPSEP 314


>SPAC186.07c |||hydroxyacid dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 332

 Score =  113 bits (271), Expect = 7e-26
 Identities = 79/246 (32%), Positives = 126/246 (51%), Gaps = 20/246 (8%)

Query: 62  EIHEKVLNEAVGALMWHTIILT-----KEDLEKFKAL-----RIIVRIGSGVDNIDVKAA 111
           + HE +LN+    L     ++      K D +  K L     ++I    +G +N+D+KAA
Sbjct: 28  DYHESILNKKTAVLAEKAPVVCVFVNDKVDADTLKVLAKNGTKLIALRCAGFNNVDLKAA 87

Query: 112 GELGIAVCNVPGYGVEEVADTTMCLILNLYRRTYWLANMVREGKKFTGPEQVREASAGCA 171
            + GI V  VP Y    VA+ T+ L+L+L R+ +     VRE       +          
Sbjct: 88  ADNGITVVRVPAYSPYAVAEYTIGLLLSLNRKIHRAYVRVRE-------DDFNLNGLLGH 140

Query: 172 RIRGDTLGIVGLGRIGSAVALRAK-AFGFNVIFYDPYLPDGIEKSLGLTRVYTLQDLLFQ 230
            + G T+G++G GRIG  VA   K  FG  V+ +D      +EK  G+  V   Q++L +
Sbjct: 141 DLHGKTIGLLGTGRIGGLVAKCLKLGFGCEVLAHDIKPNKELEK-FGIQFV-EQQEVLAK 198

Query: 231 SDCVSLHCSLNEHNHHLINEFTIKQMRPGAFLVNTARGGLVDDEGLAAALKQGRIRAAAL 290
           +D + LHC L     HL++E  +  M+ G  ++NT+RGGLVD + L  A++ G++   A+
Sbjct: 199 ADFLCLHCPLTPDTEHLVDEKLLASMKKGVKIINTSRGGLVDTKALVKAIESGQVGGCAM 258

Query: 291 DVHENE 296
           DV+E E
Sbjct: 259 DVYEGE 264


>SPAC186.02c |||hydroxyacid dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 332

 Score =  109 bits (262), Expect = 8e-25
 Identities = 72/204 (35%), Positives = 114/204 (55%), Gaps = 13/204 (6%)

Query: 95  IIVRIGSGVDNIDVKAAGELGIAVCNVPGYGVEEVADTTMCLILNLYRRTYWLANMVREG 154
           + +R G G +N+++KAA E  I V +VP Y    V++ T+ L+L+L R+ +     VRE 
Sbjct: 72  VALRCG-GYNNVNLKAASEYKITVVHVPSYSPFAVSEFTVGLLLSLNRKIHRAYVRVRED 130

Query: 155 K-KFTGPEQVREASAGCARIRGDTLGIVGLGRIGSAVALRAK-AFGFNVIFYDPYLPDGI 212
                G         GC  I G T+G++G G+IGS VA   K  FG +V+ YD   PD  
Sbjct: 131 DFNIVG-------LLGCD-IHGKTVGVIGTGKIGSNVAKCFKMGFGCDVLAYD-INPDKK 181

Query: 213 EKSLGLTRVYTLQDLLFQSDCVSLHCSLNEHNHHLINEFTIKQMRPGAFLVNTARGGLVD 272
            ++ G+  V    ++L ++D + LHC L     H++N  ++  M+ G  +VNT+RGGL+D
Sbjct: 182 LENYGVQFVEQ-NEVLKKADFLCLHCPLTPSTTHIVNSDSLALMKKGVTIVNTSRGGLID 240

Query: 273 DEGLAAALKQGRIRAAALDVHENE 296
            + L  A+  G++   A+DV+E E
Sbjct: 241 TKALVDAIDSGQVGGCAIDVYEGE 264


>SPACUNK4.10 |||hydroxyacid dehydrogenase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 334

 Score =  105 bits (252), Expect = 1e-23
 Identities = 73/219 (33%), Positives = 116/219 (52%), Gaps = 12/219 (5%)

Query: 81  ILTKEDLEKFK-ALRIIVRIGSGVDNIDVKAAGELGIAVCNVPGYGVEEVADTTMCLILN 139
           I  KE ++    +++ I  +G+G + +DV A    GI V +VP    +  AD  + L+L 
Sbjct: 68  IFDKEIIDNLPPSVKFICHLGAGYETVDVAACTARGIQVSHVPKAVDDATADVGIFLMLG 127

Query: 140 LYRRTYWLANMVREGKKFTGPEQVREASAGCARIRGDTLGIVGLGRIGSAVALRAKAFGF 199
             R        + E  K       + +        G TLGI+GLG IG  +A RA+AF  
Sbjct: 128 ALRG---FNQGIFELHKNNWNANCKPSHDP----EGKTLGILGLGGIGKTMAKRARAFDM 180

Query: 200 NVIFYDPY-LPDGIEKSLGLTRVYTLQDLLFQSDCVSLHCSLNEHNHHLINEFTIKQMRP 258
            +++++   LP+  E++ G   V +  DLL +SD +SL+  LN H  H+I +   ++M+ 
Sbjct: 181 KIVYHNRTPLPE--EEAEGAEFV-SFDDLLAKSDVLSLNLPLNAHTRHIIGKPEFQKMKR 237

Query: 259 GAFLVNTARGGLVDDEGLAAALKQGRIRAAALDVHENEP 297
           G  +VNTARG ++D+  L  AL +G + +A LDV E EP
Sbjct: 238 GIVIVNTARGAVMDEAALVEALDEGIVYSAGLDVFEEEP 276


>SPBC1773.17c ||SPBP26C9.01c|hydroxyacid dehydrogenase
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 340

 Score = 96.3 bits (229), Expect = 8e-21
 Identities = 69/248 (27%), Positives = 118/248 (47%), Gaps = 15/248 (6%)

Query: 57  AQSTSEIHEKVLNEAVGALMW---HTII--LTKEDLEKF-KALRIIVRIGSGVDNIDVKA 110
           +++  +IHE        A  W   +  I   T+E L       ++ V   +G +N+DV  
Sbjct: 42  SKTIEKIHEAASKSTFDACFWLFRNAAISPFTEEMLGPLLPTCKLFVTGAAGYNNVDVDW 101

Query: 111 AGELGIAVCNVPGYGVEEVADTTMCLILNLYRRTYWLANMVREGKKFTGPEQVREASAGC 170
           A   G+ V N P    E  A+  + L +   R        +R GK        R+  +  
Sbjct: 102 ATRNGVYVANTPNGPTEGTANMNLMLFMCTLRGAREAEQSLRLGKW-------RQNLSLT 154

Query: 171 ARIRGDTLGIVGLGRIGSAVALRAKAFGFNVIFYDPY-LPDGIEKSLGLTRVYTLQDLLF 229
               G  +GI+G+G IG + A +    G  +++++   L    EK LG + V +  +LL 
Sbjct: 155 DDPYGKRVGIIGMGAIGKSFAQKILPLGCEIVYHNRNRLEAEEEKRLGASFV-SFDELLS 213

Query: 230 QSDCVSLHCSLNEHNHHLINEFTIKQMRPGAFLVNTARGGLVDDEGLAAALKQGRIRAAA 289
            SD +S++C L    H LI+    ++M+ G +++NTARG +++++    A+K G++  A 
Sbjct: 214 SSDVISINCPLTPATHDLISTKEFEKMKDGVYIINTARGAIINEDAFIKAIKSGKVARAG 273

Query: 290 LDVHENEP 297
           LDV  NEP
Sbjct: 274 LDVFLNEP 281


>SPBC56F2.12 |ilv5||acetohydroxyacid
           reductoisomerase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 404

 Score = 27.9 bits (59), Expect = 3.4
 Identities = 13/29 (44%), Positives = 16/29 (55%)

Query: 174 RGDTLGIVGLGRIGSAVALRAKAFGFNVI 202
           + DTL I+G G  G    L A+  G NVI
Sbjct: 83  KNDTLAIIGYGSQGHGQGLNARDQGLNVI 111


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.322    0.137    0.410 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,779,912
Number of Sequences: 5004
Number of extensions: 64580
Number of successful extensions: 137
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 119
Number of HSP's gapped (non-prelim): 10
length of query: 487
length of database: 2,362,478
effective HSP length: 76
effective length of query: 411
effective length of database: 1,982,174
effective search space: 814673514
effective search space used: 814673514
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 56 (26.6 bits)