Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA002339-TA|BGIBMGA002339-PA|IPR000886|Endoplasmic
reticulum targeting sequence
(321 letters)
Database: uniref50
1,657,284 sequences; 575,637,011 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
UniRef50_Q54YW1 Cluster: Putative uncharacterized protein; n=1; ... 42 0.028
UniRef50_Q6NUA7 Cluster: Nucleotide exchange factor SIL1 precurs... 40 0.11
UniRef50_UPI00015B4D04 Cluster: PREDICTED: hypothetical protein;... 38 0.26
UniRef50_UPI0000D56F2F Cluster: PREDICTED: similar to SIL1 prote... 36 1.1
UniRef50_Q6NP53 Cluster: SD02276p; n=6; Bilateria|Rep: SD02276p ... 33 7.4
UniRef50_A3IDX2 Cluster: Methyl-accepting chemotaxis protein; n=... 33 9.8
UniRef50_A1ZM70 Cluster: Leucine-rich-repeat protein; n=1; Micro... 33 9.8
>UniRef50_Q54YW1 Cluster: Putative uncharacterized protein; n=1;
Dictyostelium discoideum AX4|Rep: Putative
uncharacterized protein - Dictyostelium discoideum AX4
Length = 977
Score = 41.5 bits (93), Expect = 0.028
Identities = 33/129 (25%), Positives = 66/129 (51%), Gaps = 4/129 (3%)
Query: 60 IESFKDKIYAKEFLKSRGIERVIVPNLMMPHLELKTSILVLVKIL-FEVTPTATKAAVPI 118
++ K++I K+F++ GIE ++ + L ++ L I+ +E T T+ +
Sbjct: 158 LKDLKEEILIKKFVEKNGIEVIVCQLKELTGNTLSYALSALQTIMSYEFTITSMTSTDTA 217
Query: 119 ALVDRLLDIFENDGNLALKAHSVDILVLWLPKNPLLQARVMKLKGLEPFYNQITKLNGSI 178
+L+ +LL + EN N ++ S+ +L L+L ++ L + L YN+ TK N +
Sbjct: 218 SLITQLLPLTENTSNPSISKTSLSLLCLFLNQSNNLNFKQFS-STLVLEYNEKTKRNYN- 275
Query: 179 VKTLLELFN 187
TL++L +
Sbjct: 276 -HTLVQLLS 283
>UniRef50_Q6NUA7 Cluster: Nucleotide exchange factor SIL1 precursor;
n=1; Xenopus laevis|Rep: Nucleotide exchange factor SIL1
precursor - Xenopus laevis (African clawed frog)
Length = 456
Score = 39.5 bits (88), Expect = 0.11
Identities = 43/228 (18%), Positives = 96/228 (42%), Gaps = 11/228 (4%)
Query: 10 EPDAVTEIAKHIEDIEKLSKAAAKANDGVQIPIQD-TNII--YEGIIQVIQTIIESFKDK 66
E D + +I + IE L KA N V+ + T I+ + + + + +
Sbjct: 148 EKDYLQDIKQRFRPIEDLQKAFNDLNINVETDFEIMTKIVNRFNSSSSTTEKVSALYDLE 207
Query: 67 IYAKEFLKSRGIERVIVPNLMMPHLELKTSILV-----LVKILFEVTPTATKAAVPIALV 121
Y + ++ + ++ L++ L ++L+ ++ P A +
Sbjct: 208 YYVHQVDNAQNLLKLGALQLLINSLNSTDTLLIENSAFVIGSALSSNPKVQIEAFEAGAL 267
Query: 122 DRLLDIFENDGNLALKAHSVDILVLWLPKNPLLQARVMKLKGLEPFYNQITKLNGS--IV 179
+LL I D +++K ++ L L + P Q R MKL GL+ N + N +
Sbjct: 268 QKLLVILAADQEVSVKKKTLYALSSMLRQFPYAQQRFMKLGGLQILKNFFKEKNAESLYI 327
Query: 180 KTLLELFNVILKEHIRARDGKIQKGKNDKSRLYQRIGLIERMSTPPVC 227
+ + L+++I+++ + ++ ++ K + Y +I L+E ++ C
Sbjct: 328 RVITLLYDMIMEKMLLYKENNTEQ-YEQKYQQYNQINLLESITEQGWC 374
>UniRef50_UPI00015B4D04 Cluster: PREDICTED: hypothetical protein;
n=1; Nasonia vitripennis|Rep: PREDICTED: hypothetical
protein - Nasonia vitripennis
Length = 471
Score = 38.3 bits (85), Expect = 0.26
Identities = 36/188 (19%), Positives = 75/188 (39%), Gaps = 1/188 (0%)
Query: 44 DTNIIYEGIIQVIQTIIESFKDKIYAKEFLKSRGIERVIVPNLMMPHLELKTSILVLVKI 103
D N + E I++++ + A+ F G+ ++I P+L + E+K L L+
Sbjct: 196 DANKV-EDILEILNNLEYLIHQIDNAQLFTDMGGMAKIISPSLNSTNWEVKAEALKLLGA 254
Query: 104 LFEVTPTATKAAVPIALVDRLLDIFENDGNLALKAHSVDILVLWLPKNPLLQARVMKLKG 163
+ P A+ V +LL + + +K+ + L + P Q ++ G
Sbjct: 255 AVQSNPKVQLKALESDFVQKLLHMLTVHNKVEVKSRCLFALGALVRHFPAAQKALVNNGG 314
Query: 164 LEPFYNQITKLNGSIVKTLLELFNVILKEHIRARDGKIQKGKNDKSRLYQRIGLIERMST 223
LE F + + +L L N + E ++ + ++ + ++R Y ++
Sbjct: 315 LEVFGKILIDGQSQVQTRVLNLINDLTIERQNLKEIQDEQQRLRRTREYDLTNFEPKLLM 374
Query: 224 PPVCSGLV 231
C LV
Sbjct: 375 HSYCQNLV 382
>UniRef50_UPI0000D56F2F Cluster: PREDICTED: similar to SIL1 protein
precursor; n=1; Tribolium castaneum|Rep: PREDICTED:
similar to SIL1 protein precursor - Tribolium castaneum
Length = 379
Score = 36.3 bits (80), Expect = 1.1
Identities = 39/191 (20%), Positives = 77/191 (40%), Gaps = 1/191 (0%)
Query: 3 EIDPFTNEPDAVTEIAKHIEDIEKLSKAAAKANDGVQIPIQDTNIIYEGIIQVIQTIIES 62
E+ NE + EI K + D++ K A+ + Q+ + ++++++ +
Sbjct: 74 ELKKIKNEFRSYDEIKKTLGDLKLTPKMDAEIIADLLQRHQEV-VDKTELLKILEDLDFL 132
Query: 63 FKDKIYAKEFLKSRGIERVIVPNLMMPHLELKTSILVLVKILFEVTPTATKAAVPIALVD 122
A+EF+K G +I NL E+K L L+ L + A+ V
Sbjct: 133 AHQYDNAREFVKQNGFREMIYKNLNSTDSEVKKETLKLMTALMQNNVNPKIHALESGAVG 192
Query: 123 RLLDIFENDGNLALKAHSVDILVLWLPKNPLLQARVMKLKGLEPFYNQITKLNGSIVKTL 182
LL + + +L +K ++ L L P Q + ++ GL + + L
Sbjct: 193 VLLRLVNFESDLGVKTRALSALGALLRSFPAAQRKFVESGGLSVLSKFFDSDDIKLQIKL 252
Query: 183 LELFNVILKEH 193
+ + + +L EH
Sbjct: 253 VTMISDLLVEH 263
>UniRef50_Q6NP53 Cluster: SD02276p; n=6; Bilateria|Rep: SD02276p -
Drosophila melanogaster (Fruit fly)
Length = 972
Score = 33.5 bits (73), Expect = 7.4
Identities = 40/142 (28%), Positives = 60/142 (42%), Gaps = 9/142 (6%)
Query: 17 IAKHIEDIEKLSKAAAKANDGVQIPIQDTNIIYEGII----QVIQTIIESFKDKIYAKEF 72
+A H+ DI K A + PIQ I G + Q IQ + ES + K
Sbjct: 462 LAPHVHDIIKSDAKKPLAEENFSYPIQSAIIASNGQLTEGFQYIQELRESQSHRSRHKME 521
Query: 73 LKSRGIERVIVPNLMM---PHLE-LKTSILVLVKILFEVTPTATKAAVPIALVDRL-LDI 127
S ++V+V M P +E L V + + +V A + A A VD + LD+
Sbjct: 522 GSSESDKKVLVLGAGMVSAPLVEWLHREKDVSITVCSQVKEEADRLAQQYAGVDSVYLDV 581
Query: 128 FENDGNLALKAHSVDILVLWLP 149
E+ G+L D++V LP
Sbjct: 582 NESTGHLQELCGRADVVVSLLP 603
>UniRef50_A3IDX2 Cluster: Methyl-accepting chemotaxis protein; n=1;
Bacillus sp. B14905|Rep: Methyl-accepting chemotaxis
protein - Bacillus sp. B14905
Length = 582
Score = 33.1 bits (72), Expect = 9.8
Identities = 27/104 (25%), Positives = 48/104 (46%), Gaps = 4/104 (3%)
Query: 14 VTEIAKHIEDIEKLSKAAAKANDGVQIPIQDTNIIYEGIIQVIQTIIESFK--DKIYAKE 71
V+E K +E +E + A K ND V + N + + + Q++++I+ K D + +
Sbjct: 462 VSETTKTVETVEGTMETAKKLNDDVVLTQSKFNQMSDSVKQIVESILAVNKEMDVMTSYS 521
Query: 72 FLKSRGIERV--IVPNLMMPHLELKTSILVLVKILFEVTPTATK 113
L S GIE + E+ +SI +K + +V A K
Sbjct: 522 KLMSEGIESASSVSEQTAASVQEIASSIDEHIKAITDVAHAAEK 565
>UniRef50_A1ZM70 Cluster: Leucine-rich-repeat protein; n=1;
Microscilla marina ATCC 23134|Rep: Leucine-rich-repeat
protein - Microscilla marina ATCC 23134
Length = 317
Score = 33.1 bits (72), Expect = 9.8
Identities = 41/141 (29%), Positives = 67/141 (47%), Gaps = 15/141 (10%)
Query: 53 IQVIQTIIESFKDKIYAKEFLKSRGIERVIVPN--LMMPHLELKTSILVLVKILFEVTPT 110
I++I I+S K+ KEF + I+P+ L +PHL+ IL L + LF P
Sbjct: 59 IEIIPDEIKSLKN---LKEFHLGFA-QLTILPDALLELPHLK----ILGLTRNLFTEVPE 110
Query: 111 ATK---AAVPIALVDRLLDIFENDGNLALKAHSVDILVLWLPKNPLLQARVMKLKGLEPF 167
A ++L LL+ F D ++ + HS+DI + P ++ KL+ L
Sbjct: 111 QVMKMTALENLSLAGNLLESFPEDMSIFTELHSLDISGNDFAEIPASVFQLSKLEELYAN 170
Query: 168 YNQITKLNGSI--VKTLLELF 186
+N +T + I +K L EL+
Sbjct: 171 FNLLTAIPEEIANLKELKELY 191
Database: uniref50
Posted date: Oct 5, 2007 11:19 AM
Number of letters in database: 575,637,011
Number of sequences in database: 1,657,284
Lambda K H
0.322 0.140 0.392
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 307,102,911
Number of Sequences: 1657284
Number of extensions: 11129636
Number of successful extensions: 30360
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 30358
Number of HSP's gapped (non-prelim): 7
length of query: 321
length of database: 575,637,011
effective HSP length: 101
effective length of query: 220
effective length of database: 408,251,327
effective search space: 89815291940
effective search space used: 89815291940
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 72 (33.1 bits)
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