Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA002199-TA|BGIBMGA002199-PA|IPR000873|AMP-dependent
synthetase and ligase
(150 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 114 5e-27
SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces... 83 2e-17
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 54 7e-09
SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1 |Schiz... 54 9e-09
SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schi... 37 0.001
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 32 0.032
SPACUNK4.17 |||NAD binding dehydrogenase family protein|Schizosa... 26 2.1
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 26 2.8
SPBC12C2.04 |||NAD binding dehydrogenase family protein|Schizosa... 25 3.7
SPAC2F7.14c |||exosome subunit Rrp4 |Schizosaccharomyces pombe|c... 25 4.9
SPBC3B9.07c |rpa43|rpa21|DNA-directed RNA polymerase I complex s... 25 6.5
SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit ... 25 6.5
SPBC244.02c |||U3 snoRNP-associated protein Utp6 |Schizosaccharo... 24 8.5
SPAC1D4.12 |rad15|rhp3|transcription factor TFIIH complex subuni... 24 8.5
>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 512
Score = 114 bits (275), Expect = 5e-27
Identities = 56/136 (41%), Positives = 85/136 (62%)
Query: 8 VMKGYHKNPAATKDTITEDGYFKTGDLGYYKPGVGLFITDRIKELIKVKGMQVAPAELES 67
V KGY NPAA K + T+D +F+TGD G +FIT RIKEL+ G +++PAE+++
Sbjct: 363 VTKGYLNNPAANKSSFTKDRFFRTGDEGKLDKDGYVFITGRIKELVNRGGEKISPAEIDA 422
Query: 68 LLRSHPAVADAAVIGVPHEFYGETPKAFVIRKNGHDISEKELQAHVASKVAVYKQIEEVA 127
+L HP V++A VP E YG+ +A + G ++ K+L ++ KVA +K ++
Sbjct: 423 VLMQHPDVSEAVCFAVPDEKYGQDIQAAINPVAGKTVTPKQLHDYLEQKVAAFKIPKKFY 482
Query: 128 FVDAIPKTASGKILRK 143
F D IPKTA+GK+ R+
Sbjct: 483 FTDRIPKTATGKVQRR 498
>SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 662
Score = 82.6 bits (195), Expect = 2e-17
Identities = 45/127 (35%), Positives = 77/127 (60%), Gaps = 4/127 (3%)
Query: 27 GYFKTGDLGYYKPGVGLFITDRIKELIKVKGMQVAPAELESLLRSHPAVADAAVIGVPHE 86
G++ TGD ++I R+ +++ + G +++ AE+E+ L SH AVA++AV+GV E
Sbjct: 504 GFYFTGDGATRDKDGYIWIRGRVDDVVNISGHRLSTAEIEAALLSHDAVAESAVVGVHDE 563
Query: 87 FYGETPKAFVIRKNGHDIS---EKELQAHVASKVAVYKQIEEVAFVDAIPKTASGKILRK 143
G+ AF++ K G++ + EKEL V S + + ++ F D +PKT SGKI+R+
Sbjct: 564 LTGQAVNAFILLKPGYEATVELEKELIMAVRSTIGPFASPRKLIFSD-LPKTRSGKIMRR 622
Query: 144 DLKKMYA 150
L+K+ A
Sbjct: 623 ILRKILA 629
>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 689
Score = 54.4 bits (125), Expect = 7e-09
Identities = 27/78 (34%), Positives = 44/78 (56%), Gaps = 1/78 (1%)
Query: 1 MYMKSPTVMKGYHKNPAATKDTITEDGYFKTGDLGYYKPGVGLFITDRIKELIK-VKGMQ 59
++++ P V GY P K TEDG+FKTGD+G G L + DR K ++K + G
Sbjct: 493 VWIRGPAVCNGYLNRPEDNKAAFTEDGWFKTGDVGEIAKGNTLRLIDRKKNIVKSLNGEY 552
Query: 60 VAPAELESLLRSHPAVAD 77
+A ++E+ + P V++
Sbjct: 553 IALEKIEAQFFTSPLVSN 570
>SPBC18H10.02 |lcf1||long-chain-fatty-acid-CoA ligase Lcf1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 676
Score = 54.0 bits (124), Expect = 9e-09
Identities = 28/81 (34%), Positives = 47/81 (58%), Gaps = 1/81 (1%)
Query: 1 MYMKSPTVMKGYHKNPAATKDTITEDGYFKTGDLGYYKPGVGLFITDRIKELIKVK-GMQ 59
++++ P++ +GY K++ T DG+F+TGD+G P L I DR K L+K + G
Sbjct: 486 IWLRGPSLTRGYLNRDKENKESFTPDGWFRTGDVGELTPEGLLRIIDRKKNLVKTQNGEY 545
Query: 60 VAPAELESLLRSHPAVADAAV 80
+A +LES R+ V++ V
Sbjct: 546 IALEKLESRYRTSSLVSNICV 566
>SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1419
Score = 36.7 bits (81), Expect = 0.001
Identities = 16/52 (30%), Positives = 29/52 (55%)
Query: 26 DGYFKTGDLGYYKPGVGLFITDRIKELIKVKGMQVAPAELESLLRSHPAVAD 77
D +++GDLG Y P + + R + IK++G ++ E+ + L HP V +
Sbjct: 718 DRMYRSGDLGRYLPTGNVECSGRADDQIKIRGFRIELGEINTHLSRHPNVRE 769
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 32.3 bits (70), Expect = 0.032
Identities = 23/74 (31%), Positives = 36/74 (48%), Gaps = 14/74 (18%)
Query: 17 AATKDTITEDGYFKTGDLGYYK----------PGVG---LFITDRIKELIKVKGMQVAPA 63
A D +GY +TGDLG+ + P V L++ I E +V G+ P+
Sbjct: 1322 ATNSDGFLGNGYVRTGDLGFLQITSHSMGPNAPVVDMQLLYVLGPIGETFEVNGLSHFPS 1381
Query: 64 ELESLL-RSHPAVA 76
++E + RSHP +A
Sbjct: 1382 DIEDTIERSHPRIA 1395
>SPACUNK4.17 |||NAD binding dehydrogenase family
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 405
Score = 26.2 bits (55), Expect = 2.1
Identities = 13/26 (50%), Positives = 16/26 (61%), Gaps = 1/26 (3%)
Query: 69 LRSHPAVA-DAAVIGVPHEFYGETPK 93
L SHP DA +IG+P E +G T K
Sbjct: 113 LDSHPEEKPDAYIIGIPPETHGSTQK 138
>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 25.8 bits (54), Expect = 2.8
Identities = 15/58 (25%), Positives = 27/58 (46%)
Query: 92 PKAFVIRKNGHDISEKELQAHVASKVAVYKQIEEVAFVDAIPKTASGKILRKDLKKMY 149
P + K G ISE+E V + + E VA+ + P +G + KD+ +++
Sbjct: 385 PTVIINAKQGMLISEEETFGPVGALFKFDTEDEVVAWANDSPVGLAGYLFSKDISRVF 442
>SPBC12C2.04 |||NAD binding dehydrogenase family
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 384
Score = 25.4 bits (53), Expect = 3.7
Identities = 14/33 (42%), Positives = 19/33 (57%), Gaps = 1/33 (3%)
Query: 60 VAPAELESLLRSHPA-VADAAVIGVPHEFYGET 91
V+ E L SHP V A +IG+P +F+G T
Sbjct: 83 VSVTEYLEYLDSHPEDVPSAYLIGIPPDFHGCT 115
>SPAC2F7.14c |||exosome subunit Rrp4 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 329
Score = 25.0 bits (52), Expect = 4.9
Identities = 15/55 (27%), Positives = 27/55 (49%), Gaps = 6/55 (10%)
Query: 10 KGYHKNPAATKDTITEDGYFKTGDLGYYKPGVGLFIT-----DRIKELIKVKGMQ 59
K H+N +T+D F G Y++ G G++ + R+ +LI VK ++
Sbjct: 51 KSIHQNLVTPGQLVTDDPQFMRGHGTYFEDG-GIYASVAGSVQRVNKLISVKPLR 104
>SPBC3B9.07c |rpa43|rpa21|DNA-directed RNA polymerase I complex
subunit Rpa43|Schizosaccharomyces pombe|chr 2|||Manual
Length = 173
Score = 24.6 bits (51), Expect = 6.5
Identities = 13/31 (41%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
Query: 21 DTITEDGYFKTGDLGYYKPGVGL-FITDRIK 50
DT E G +KT D +PG L F+ D I+
Sbjct: 128 DTTEEQGRWKTNDGNILEPGKDLEFVVDGIQ 158
>SPCC1840.02c |bgs4|orb11, cwg1|1,3-beta-glucan synthase subunit
Bgs4|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1955
Score = 24.6 bits (51), Expect = 6.5
Identities = 16/44 (36%), Positives = 23/44 (52%), Gaps = 1/44 (2%)
Query: 66 ESLLRSHPAVADAAVIGVPHEFYGETPKAFVIRKNGH-DISEKE 108
E LLR++P + A + P E G P+ F +GH +I E E
Sbjct: 1082 EFLLRAYPDLQIAYLDEDPPEEEGAEPQLFAALIDGHSEIMENE 1125
>SPBC244.02c |||U3 snoRNP-associated protein Utp6
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 488
Score = 24.2 bits (50), Expect = 8.5
Identities = 12/41 (29%), Positives = 19/41 (46%)
Query: 47 DRIKELIKVKGMQVAPAELESLLRSHPAVADAAVIGVPHEF 87
D I K+K + + + L+ HP A+ V+ HEF
Sbjct: 112 DYIHYAQKIKAVNIVGKICVAALQKHPNNAELWVVACDHEF 152
>SPAC1D4.12 |rad15|rhp3|transcription factor TFIIH complex subunit
Rad15|Schizosaccharomyces pombe|chr 1|||Manual
Length = 772
Score = 24.2 bits (50), Expect = 8.5
Identities = 9/20 (45%), Positives = 14/20 (70%)
Query: 29 FKTGDLGYYKPGVGLFITDR 48
++T LGY +P +GL +T R
Sbjct: 92 YRTSQLGYEEPFLGLGLTSR 111
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.134 0.379
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 673,601
Number of Sequences: 5004
Number of extensions: 25108
Number of successful extensions: 65
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 55
Number of HSP's gapped (non-prelim): 14
length of query: 150
length of database: 2,362,478
effective HSP length: 67
effective length of query: 83
effective length of database: 2,027,210
effective search space: 168258430
effective search space used: 168258430
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 50 (24.2 bits)
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