Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA002140-TA|BGIBMGA002140-PA|IPR000873|AMP-dependent
synthetase and ligase
(1071 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 175 2e-44
SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb... 87 2e-17
SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase |Schi... 30 2.0
SPBC1539.09c |trp1||anthranilate synthase component II|Schizosac... 29 4.6
SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|c... 28 6.1
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 175 bits (427), Expect = 2e-44
Identities = 151/579 (26%), Positives = 245/579 (42%), Gaps = 35/579 (6%)
Query: 353 HQVGIYCLALVQPNFLPKTPLGG---IHLSECKRRFLEGTLHPSNVLMCPHTCVTNLPKP 409
HQV +YC+ + P LP+ G I C+R F GTL V V NLP
Sbjct: 778 HQVRVYCVLICAPFTLPRVTKNGRQEIGNMMCRRAFEHGTLPFLYVKFAVERAVLNLPVG 837
Query: 410 REIHSDVGP--ASVIVGNLVQGNRLASAQGRDMGYTDDSDAA---RKYQFISQILRWRAQ 464
+ + AS I NL+ L + D D+ + + Q+L+ R
Sbjct: 838 EDAIDGIWSSYASGIRQNLLSDQELQYSGFIDRSLRYDAKTSVDISSCHTMLQLLQLRVA 897
Query: 465 STSDHVIFTLLNAKGAVSKVLTCAELHKKAERIGNLLLEKGRVNTGDHVALIFPPGLDLI 524
++ + + ++ +G K +T + ++ I L +K + G V L++ D +
Sbjct: 898 KNAEDIAYITIDGRGREGKNITWRKFDQRVATIIRYLQKKKYIKPGRVVVLMYTHSEDFV 957
Query: 525 CAFYGCLYVGAVPVTIRPPHPQNLHTTLPTVRMIVDVSKATLVLSNQSVIKLLRSKEASN 584
A Y C Y+G +P+ + P L +P ++ + VL N LR+K S
Sbjct: 958 YALYACFYLGLIPIPVPPLDHMRLSEDVPAFLFLIKHYYVSAVLVNSEADTALRAKTTSQ 1017
Query: 585 VLDSKAW------PMTLDTDDMPKK---------KLPIMYRAPTAEMLAYLDFSVSTTGM 629
L A P + T + K+ KL + P L + +S
Sbjct: 1018 HLKQSAMAAKVVLPSFIVTSKISKQTKSIKELNVKLDPICLDPAFPALVWAFWSPDHRLT 1077
Query: 630 LAGIKMSHAAVTSLCRSMKIACELYPSRHIALCLDPYCGLGFALWCLSSIYSGHHSILIP 689
L +H + S+C+ K C++ R + + G+GF CL ++ G + L+
Sbjct: 1078 LTAY--NHQTLLSICQIHKETCQMTHKRPLLGHVRSMSGIGFFHTCLMGVFLGTTTYLLS 1135
Query: 690 PSEVEVNPALWLSAVSQYKVRDTFCSYGVMELCTKGLGSSVNQLKSKGINLACVRTCVVV 749
P + NP L +S+YK++DT+ ++ + L NQ +K NL+C+ ++
Sbjct: 1136 PVDFANNPLLLFQIISKYKIKDTYATF-------QTLNYIQNQQPTKWPNLSCLENLMIP 1188
Query: 750 AEERPRINLTNSFSKLFSALGLSPRAVSTSFGCRVNIAICLQGASSPEPSTVYVDLRALR 809
+ R S K F GLSP A ST + +N I + P+ +DLRALR
Sbjct: 1189 HDGRISAFYIASLQKYFVKHGLSPYAFSTVYSNCLNPFISTRSYMGAIPTPQLLDLRALR 1248
Query: 810 NDRVSLVERG-SPHSLCLMESGKLLPGVKVITANPETKGQCGDSHLGEIWVQSPHNASGY 868
+ + E P++L L++SG + ++ NP+T+ C GEIW++S NA +
Sbjct: 1249 HGLIQPCESADKPYALPLLDSGMVPVSTQLAIVNPDTRELCRVGEYGEIWMRSSANAISF 1308
Query: 869 FTIYGDESDYADHFSAQLVTGNTGEVYARTGYLGFLRRT 907
F D D F+A G G Y RTG LGFL+ T
Sbjct: 1309 FQ-STDPVDMM-RFNATNSDGFLGNGYVRTGDLGFLQIT 1345
Score = 78.2 bits (184), Expect = 6e-15
Identities = 118/533 (22%), Positives = 220/533 (41%), Gaps = 89/533 (16%)
Query: 450 RKYQFISQILRWRAQSTSDHVIFTLLNAKGAVSKVLTCAELHKKAERIGNLLLEKGRVNT 509
+++ I+ +LR+R ++ F +L+ KG +T +L +AE++ ++ +K +
Sbjct: 159 KQFTNIAAVLRYRGVHSAKKTAFIILDNKGKEFTSITWEKLASRAEKVAQVIRDKSGLFR 218
Query: 510 GDHVALIFP--PGLDLICAFYGCLYVGAVPVTIRPPHPQNLHTTLPTVRMIVDVSKATLV 567
D V L++ +D + + +GC G V V P N + I+ + A L
Sbjct: 219 SDRVVLMYRDCEAIDFVVSLFGCFIAGVVAV------PINRFDDYNELSSILTTTSARLA 272
Query: 568 LSNQSVIKLLRSKEASNVLDSKAWPMTLD---TDDMPKKKLPIMYRAPTAEM--LAYLDF 622
L+ + +K + + L WP ++ T++ L P ++ LAY++F
Sbjct: 273 LTTDANLKAFQRDLNAKKLH---WPKNVEWWKTNEFGGFHLKKKAEMPPLQVPDLAYIEF 329
Query: 623 SVSTTGMLAGIKMSHAAV---TSLCRSMKIACELYPSRHI-------------------A 660
S S G L G+ +SH + + ++ Y S +
Sbjct: 330 SRSPIGELHGVVISHRTILHQMNCLAAIHATAPAYESDKLDYLSIDREYTEGLSKSGLFL 389
Query: 661 LCLDPYCGLGFALWCLSSIYSGHHSILIPPSEVEVNPALWLSAVSQYKVRDTFCSYGVME 720
LD +G L L +++SG+ ++ P + V V P LW + ++Y+ Y ++
Sbjct: 390 TYLDLRQAIGLILGVLHTVFSGYTTVWCPQNAVFV-PGLWANLATRYRASFMLTDYAGLK 448
Query: 721 LCTKGL----GSSVNQLKSKGINLACVRTCVVVAEERPRINLTNSFSKLFS-----ALG- 770
+++ K ++L+ +R C+V +N+ F ++ S LG
Sbjct: 449 TIAYNYQNDPKATLGFSKKHSVDLSSLRMCMVDC-----LNVDCEFQEIVSDRWLKPLGN 503
Query: 771 LSPRAVSTSFGC---RVNIAICL------------QGASSP-----EPSTVYVDLRALR- 809
+PRA C + I + +G SP E S V ++ AL+
Sbjct: 504 QNPRATFVPLLCLPEHGGMVISMKDWIGGEEFMSPKGFKSPRTPENEISEVLLEKEALKL 563
Query: 810 NDRVSLVE-----RGSPHSLCLMESGKLLPGVKVITA--NPETKGQCGDSHLGEIWVQSP 862
N+ V L E R S H + P V A +PET+ C + +GEIWV SP
Sbjct: 564 NEVVVLAEDDKARRQSKHPNTIRVGAFWYPFVDATLAIVDPETQVLCLPNIVGEIWVDSP 623
Query: 863 HNASGYFTIYGDESDYADHFSAQLVTGNT------GEVYARTGYLGFLRRTEI 909
+ G+F + +++ H ++ +T + + RTG LGF+R+ ++
Sbjct: 624 SLSGGFFAL-PKQTEAIFHARTSFISSDTFQPIPSNQEFLRTGLLGFIRKGKV 675
Score = 43.6 bits (98), Expect = 2e-04
Identities = 22/58 (37%), Positives = 35/58 (60%), Gaps = 2/58 (3%)
Query: 952 VFVVGALDETIMLRGMRYHPIDIENSVMRCHKKIAE--CAVFTWTNLLVVVVELDGND 1007
++V+G + ET + G+ + P DIE+++ R H +IA AVF +VVV+E G D
Sbjct: 1361 LYVLGPIGETFEVNGLSHFPSDIEDTIERSHPRIARGGTAVFQSAGRVVVVIEALGQD 1418
Score = 29.5 bits (63), Expect = 2.6
Identities = 14/35 (40%), Positives = 19/35 (54%)
Query: 207 LCKTDEVGEICVLSGATGSGYWGLPGLTNTVFRVR 241
LC + VGEI V S + G++ LP T +F R
Sbjct: 609 LCLPNIVGEIWVDSPSLSGGFFALPKQTEAIFHAR 643
>SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1428
Score = 86.6 bits (205), Expect = 2e-17
Identities = 96/465 (20%), Positives = 182/465 (39%), Gaps = 27/465 (5%)
Query: 455 ISQILRWRAQSTSDHVIFTLLNAKGAVSKVLTCAELHKKAERIGNLLLEKGRVNTGDHVA 514
I L+ ++ T NA LT A K + + H+
Sbjct: 802 ILDFLKTKSAKTPTSPAILTFNAIEKTKVELTWAHFELKVASHVEYMQLSVKAKARSHIL 861
Query: 515 LIFPPGLDLICAFYGCLYVGAVPVTIRPPHPQNLHTTLPTVRMIVDVSKATLVLSNQSVI 574
L++ L+ + + + C ++G +P+ + L + I +L + V+
Sbjct: 862 LLYYDPLEFLISIHSCFHLGVIPIPFQIREISQLIGEIEEFTKIAKAFNVEAILVDHKVL 921
Query: 575 KLLRSKEASNV-------LDSKAWPMTLDTDDMP-----KKKLPIMYRAPTAEM--LAYL 620
L+S++ SN L+ KA P +T +P KK L ++ +A +
Sbjct: 922 ISLKSRDISNHFQQTCIDLNMKA-PKIFETTGIPISKRAKKALNLITPGELNNKGKVALI 980
Query: 621 DFSVSTTGMLAGIKMSHAAVTSLCRSMKIACELYPSRHIALCLDPYCGLGFALWCLSSIY 680
+ G + + SH+++ + C K + I ++ G+G L +Y
Sbjct: 981 SINKLEDGSIIPTQFSHSSLLAFCHEQKEFVIGNEEKPIIGGIEFSSGMGLLHTALLGVY 1040
Query: 681 SGHHSILIPPSEVEVNPALWLSAVSQYKVRDTFCSYGVMELCTKGLGSSVNQLKSKGINL 740
+G ++LI + N +L A+ Q + + +C K S+ S IN
Sbjct: 1041 AGVPTLLIKQENLCNNGSLLFEAIEQNSLSKVMIP---LNICQKSF-STAQGCNSIVIN- 1095
Query: 741 ACVRTCVVVAEERPRINLTNSFSKLFSALGLSPRAVSTSFGCRVNIAICLQGASSPEPST 800
+ + + +V +RP + NS + + +GL+P V ++ +N +C SS E
Sbjct: 1096 STLSSIIVPCYDRPISSRVNSIIEDIARIGLAPNKVKLAYSHPINPFVCWNADSSMEKMK 1155
Query: 801 VYVDLRALRNDRVSLVERGSPHSLCLM-ESGKLLPGVKVITANPETKGQCGDSHLGEIWV 859
Y D LR+ V++ E + L+ SG ++ +PE K C + +GEIW+
Sbjct: 1156 DYFDANQLRSGLVNVREDVLRNRQPLLYGSGTSTLYNEICIVHPEEKYICQEGEIGEIWI 1215
Query: 860 QSPHNASGYFTIYGDESDYADHFSAQLVTGNTGEVYARTGYLGFL 904
H + Y + ++ + + Y+RTG LGF+
Sbjct: 1216 NGKHGS------YCENNELNSGCELLVQETLDKKSYSRTGQLGFI 1254
Score = 36.7 bits (81), Expect = 0.017
Identities = 82/454 (18%), Positives = 173/454 (38%), Gaps = 54/454 (11%)
Query: 453 QFISQILRWRAQSTSDHVIFTLLNAKGAVSKVLTCAELHKKAERIGNLLLEKGRVNTGDH 512
Q I +L +R ++ KG + + ++ +A++ ++ + G
Sbjct: 94 QDIISVLEYRRNKNPTSTAHICIDKKGKEAVSYSWDKILNRAKQFASVAQNHVGLKPGTR 153
Query: 513 VALIF--PPGLDLICAFYGCLYVGAVPVTIRPPHPQNLHTTLPTVRMIVDVSKATLVLSN 570
V L + + I + +GCL +G V + + P ++ +++++V+ K + +
Sbjct: 154 VILYYRKSEASEYIISIFGCLLLGIVVIPLSPS------SSSESLKLVVNEEKVRVAFTT 207
Query: 571 QSVIKLLRSKEASNVLDSKAWPMTLDTDDMPKKKLPIMYRAPTAEMLAYLDFSVSTTGML 630
++ ++ N S AW + D + +++ +L FS ST +
Sbjct: 208 EATYRIFIKDTEVNNAKSLAWWKSNDFTNYKFEQIKQYRMRANDTVLIDYTFSSSTYNDI 267
Query: 631 AGIKMSHAAVTSLCRSMKIACELYPSRHIALCLD--------------PYCGLGFALWCL 676
+ + ++ + R++ + P+R L P LG +
Sbjct: 268 KAVYTTKTFLSQM-RNLTSSMITNPARQAGWKLHNFEDSKDVLITNFHPSMPLGLIMGVF 326
Query: 677 SSIYSGHHSILIPPSEVEVNPALWLSAVSQYKVRDTFCSYGVMELCTKGLG----SSVNQ 732
+S+++G+ +I EV P L +++Y+ + Y ++ S+++
Sbjct: 327 NSVFAGYCTIFCD-EEVLKTPGLLAYLITKYRCTYSLFDYAGLKQTVYNYQEDPKSTLSF 385
Query: 733 LKSKGINLACVRTCVVVAEE-RPRINLTNSFSKLFSALGLS-PRAVSTSFGCRVNIA--- 787
K+ NL+ ++ C+V E P N+ S + LG + + V T C
Sbjct: 386 KKNYTPNLSSLKLCMVECEVVDPEFNIIVS-DRWLQPLGTNNSKEVITPILCLRKFGGIP 444
Query: 788 -------ICLQGASSPE---PSTVYVDLRALRN----DRVSLV-----ERGSPHS-LCLM 827
I E T Y ++ L++ ++V +V + SP+ LC+
Sbjct: 445 ISFKDWMISYTNRQKHERQMADTCYQEILVLKSSIEKEKVEIVPFLDIRKYSPNEVLCMS 504
Query: 828 ESGKLLPGVKVITANPETKGQCGDSHLGEIWVQS 861
+P V N ++K C +GEIWV S
Sbjct: 505 PFWYPIPEASVAVVNEDSKNICKVGEVGEIWVYS 538
>SPAP7G5.04c |lys1||aminoadipate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1419
Score = 29.9 bits (64), Expect = 2.0
Identities = 16/51 (31%), Positives = 25/51 (49%)
Query: 188 GQVMPSCVIVVVKMEGPAYLCKTDEVGEICVLSGATGSGYWGLPGLTNTVF 238
G+ M + ++V+ +C EVGEI + +G GY G LT+ F
Sbjct: 638 GRGMKNVQLLVINRFDTNKICGIGEVGEIYLRAGGLAEGYLGNDELTSKKF 688
Score = 29.1 bits (62), Expect = 3.5
Identities = 18/58 (31%), Positives = 28/58 (48%), Gaps = 3/58 (5%)
Query: 491 HKKAERIGNLL---LEKGRVNTGDHVALIFPPGLDLICAFYGCLYVGAVPVTIRPPHP 545
+++ + N+L L K + GD V + G+DL+ A G L GA I P +P
Sbjct: 315 YRQIDESSNILAHHLVKNGIERGDVVMVYAYRGVDLVVAVMGVLKAGATFSVIDPAYP 372
Score = 28.7 bits (61), Expect = 4.6
Identities = 14/30 (46%), Positives = 17/30 (56%)
Query: 353 HQVGIYCLALVQPNFLPKTPLGGIHLSECK 382
H++G+Y LV FLPK L G L E K
Sbjct: 1369 HEMGLYLSYLVAIGFLPKPTLEGKKLPEVK 1398
>SPBC1539.09c |trp1||anthranilate synthase component
II|Schizosaccharomyces pombe|chr 2|||Manual
Length = 759
Score = 28.7 bits (61), Expect = 4.6
Identities = 17/56 (30%), Positives = 28/56 (50%), Gaps = 5/56 (8%)
Query: 923 LPRDSDTDSLASACGSVSILPDSHD----THDAVFVVGALDETIMLRGMRYHPIDI 974
+P++ S G +S LPD D T + V ++GA + + G++YHP I
Sbjct: 147 IPQNISVTRYHSLAGKISSLPDCLDVTSWTENGV-IMGARHKKYAIEGVQYHPESI 201
>SPAC23C11.16 |plo1||Polo kinase Plo1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 683
Score = 28.3 bits (60), Expect = 6.1
Identities = 16/62 (25%), Positives = 33/62 (53%), Gaps = 2/62 (3%)
Query: 451 KYQFISQILRWRAQSTSDHVIFTLLNAKGAVSKVLTCAELHKKAERIGNLLL-EKGRVNT 509
+++ + ++LR R Q T V + ++ GA+ + +H+ ++GN++L E V
Sbjct: 121 EHKSLMELLRKRKQLTEPEVRYLMMQILGALKYMHKKRVIHRDL-KLGNIMLDESNNVKI 179
Query: 510 GD 511
GD
Sbjct: 180 GD 181
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.322 0.135 0.415
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,432,564
Number of Sequences: 5004
Number of extensions: 172439
Number of successful extensions: 356
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 3
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 331
Number of HSP's gapped (non-prelim): 16
length of query: 1071
length of database: 2,362,478
effective HSP length: 80
effective length of query: 991
effective length of database: 1,962,158
effective search space: 1944498578
effective search space used: 1944498578
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 59 (27.9 bits)
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