Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA002140-TA|BGIBMGA002140-PA|IPR000873|AMP-dependent
synthetase and ligase
(1071 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 / 4-coumaroyl... 42 0.003
At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 / 4-coumaroyl... 41 0.004
At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 / 4-coumaroyl... 41 0.004
At1g20480.1 68414.m02552 4-coumarate--CoA ligase family protein ... 36 0.14
At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein ... 35 0.25
At3g48990.1 68416.m05351 AMP-dependent synthetase and ligase fam... 33 1.3
At5g38120.1 68418.m04592 4-coumarate--CoA ligase family protein ... 32 1.8
At5g36880.1 68418.m04418 acetyl-CoA synthetase, putative / aceta... 32 1.8
At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative / 4-c... 32 1.8
At2g47830.2 68415.m05970 cation efflux family protein / metal to... 32 2.4
At2g47830.1 68415.m05969 cation efflux family protein / metal to... 32 2.4
At1g20490.1 68414.m02553 AMP-dependent synthetase and ligase fam... 32 2.4
At4g21170.1 68417.m03062 pentatricopeptide (PPR) repeat-containi... 31 3.1
At3g47570.1 68416.m05179 leucine-rich repeat transmembrane prote... 30 9.5
At1g71060.1 68414.m08201 pentatricopeptide (PPR) repeat-containi... 30 9.5
>At3g21240.1 68416.m02684 4-coumarate--CoA ligase 2 /
4-coumaroyl-CoA synthase 2 (4CL2) identical to SP|Q9S725
4-coumarate--CoA ligase 2 (EC 6.2.1.12) (4CL 2)
(4-coumaroyl-CoA synthase 2) {Arabidopsis thaliana}
Length = 556
Score = 41.5 bits (93), Expect = 0.003
Identities = 50/212 (23%), Positives = 94/212 (44%), Gaps = 22/212 (10%)
Query: 479 GAVSKVLTCAELHKKAERIGNLLLEKGRVNTGDHVALIFPPGLDLICAFYGCLYVGAVPV 538
G +V T A++H + ++ L G V D V ++ P +++ F ++GA+
Sbjct: 58 GPTGEVYTYADVHVTSRKLAAGLHNLG-VKQHDVVMILLPNSPEVVLTFLAASFIGAITT 116
Query: 539 TIRPPHPQNLHTTLPTVRMIVDVSKATLVLSNQSVIKLLRSKEASNVL----DSKAWPMT 594
+ P T + S A L+++ + +++ + VL DS A P
Sbjct: 117 SANP------FFTPAEISKQAKASAAKLIVTQSRYVDKIKNLQNDGVLIVTTDSDAIPEN 170
Query: 595 -LDTDDMPKKKLPIMYRAP---TAEMLAYLDFSVSTTGMLAGIKMSH-AAVTSLCRSMK- 648
L ++ + + P + P + E + L FS TTG+ G+ ++H VTS+ + +
Sbjct: 171 CLRFSELTQSEEPRVDSIPEKISPEDVVALPFSSGTTGLPKGVMLTHKGLVTSVAQQVDG 230
Query: 649 IACELYPSR-HIALCLDPYCGLGFALWCLSSI 679
LY +R + LC+ P F ++ L+SI
Sbjct: 231 ENPNLYFNRDDVILCVLPM----FHIYALNSI 258
>At1g65060.2 68414.m07376 4-coumarate--CoA ligase 3 /
4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
(4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
Length = 495
Score = 41.1 bits (92), Expect = 0.004
Identities = 53/210 (25%), Positives = 93/210 (44%), Gaps = 20/210 (9%)
Query: 479 GAVSKVLTCAELHKKAERIGNLLLEKGRVNTGDHVALIFPPGLDLICAFYGCLYVGAVPV 538
G+ K T E H R+ + L + G + GD + ++ + + +F G +GAV
Sbjct: 70 GSTGKSYTYGETHLICRRVASGLYKLG-IRKGDVIMILLQNSAEFVFSFMGASMIGAVST 128
Query: 539 TIRPPH-PQNLHTTLPT--VRMIVDVSKATLVLSNQSV-IKLLRSKEASNVLDSKAWPM- 593
T P + Q L+ L + ++I+ S+ L N + L+ + E + P
Sbjct: 129 TANPFYTSQELYKQLKSSGAKLIITHSQYVDKLKNLGENLTLITTDEPT---PENCLPFS 185
Query: 594 TLDTDDMPKKKLPIMYRAPT-AEMLAYLDFSVSTTGMLAGIKMSH-AAVTSLCRSMK-IA 650
TL TDD + P + A L FS TTG+ G+ ++H + +TS+ + +
Sbjct: 186 TLITDD---ETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDN 242
Query: 651 CELY-PSRHIALCLDPYCGLGFALWCLSSI 679
LY S + LC+ P F ++ L+S+
Sbjct: 243 PNLYLKSNDVILCVLPL----FHIYSLNSV 268
>At1g65060.1 68414.m07375 4-coumarate--CoA ligase 3 /
4-coumaroyl-CoA synthase 3 (4CL3) identical to SP|Q9S777
4-coumarate--CoA ligase 3 (EC 6.2.1.12) (4CL 3)
(4-coumaroyl-CoA synthase 3) {Arabidopsis thaliana}
Length = 561
Score = 41.1 bits (92), Expect = 0.004
Identities = 53/210 (25%), Positives = 93/210 (44%), Gaps = 20/210 (9%)
Query: 479 GAVSKVLTCAELHKKAERIGNLLLEKGRVNTGDHVALIFPPGLDLICAFYGCLYVGAVPV 538
G+ K T E H R+ + L + G + GD + ++ + + +F G +GAV
Sbjct: 70 GSTGKSYTYGETHLICRRVASGLYKLG-IRKGDVIMILLQNSAEFVFSFMGASMIGAVST 128
Query: 539 TIRPPH-PQNLHTTLPT--VRMIVDVSKATLVLSNQSV-IKLLRSKEASNVLDSKAWPM- 593
T P + Q L+ L + ++I+ S+ L N + L+ + E + P
Sbjct: 129 TANPFYTSQELYKQLKSSGAKLIITHSQYVDKLKNLGENLTLITTDEPT---PENCLPFS 185
Query: 594 TLDTDDMPKKKLPIMYRAPT-AEMLAYLDFSVSTTGMLAGIKMSH-AAVTSLCRSMK-IA 650
TL TDD + P + A L FS TTG+ G+ ++H + +TS+ + +
Sbjct: 186 TLITDD---ETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDN 242
Query: 651 CELY-PSRHIALCLDPYCGLGFALWCLSSI 679
LY S + LC+ P F ++ L+S+
Sbjct: 243 PNLYLKSNDVILCVLPL----FHIYSLNSV 268
>At1g20480.1 68414.m02552 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to
SP|Q9S725 from Arabidopsis thaliana and SP|P17814 from
Oryza sativa; contains Pfam AMP-binding enzyme domain
PF00501
Length = 565
Score = 35.9 bits (79), Expect = 0.14
Identities = 16/41 (39%), Positives = 23/41 (56%)
Query: 829 SGKLLPGVKVITANPETKGQCGDSHLGEIWVQSPHNASGYF 869
SG L P V+ +P+T G + GE+W++SP GYF
Sbjct: 385 SGLLAPNVEGKIVDPDTGRVLGVNQTGELWIRSPTVMKGYF 425
>At5g63380.1 68418.m07955 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL2
[gi:12229665] from Arabidopsis thaliana, 4CL1
[gi:12229631] from Nicotiana tabacum; contains Pfam
AMP-binding enzyme domain PF00501
Length = 562
Score = 35.1 bits (77), Expect = 0.25
Identities = 53/232 (22%), Positives = 95/232 (40%), Gaps = 20/232 (8%)
Query: 485 LTCAELHKKAERIGNLLLEKGRVNTGDHVALIFPPG-LDLICAFYGCLYVGAVPVTIRPP 543
LT EL ++ + L E+ +VA I P LD+ + + +G V + P
Sbjct: 78 LTYGELLRRVRSLAVSLRERFPSLASRNVAFILSPSSLDIPVLYLALMSIGVV---VSPA 134
Query: 544 HPQNLHTTLPTVRMIVDVSKATLVLSNQSVIKLLRSKE---ASNVLDSKAWPMTLDTDDM 600
+P + V V+VS+ + + +K L+S + ++DS + L+ D
Sbjct: 135 NPIGSESE---VSHQVEVSEPVIAFATSQTVKKLQSSSLPLGTVLMDSTEFLSWLNRSDS 191
Query: 601 PKKKLPIMYRAPTAEMLAYLDFSVSTTGMLAGIKMSH---AAVTSLCRSMKIACELYPSR 657
P + ++ A L FS TTG + G+ ++H A T++ + + R
Sbjct: 192 SSVN-PFQVQVNQSDPAAIL-FSSGTTGRVKGVLLTHRNLIASTAVSHQRTLQDPVNYDR 249
Query: 658 HIALCLDPYCGLGFALWCLSSIYSGHHSILIPPSEVEVNPALWLSAVSQYKV 709
L + GF + + +I G +L+ E+E AV +YKV
Sbjct: 250 VGLFSLPLFHVFGF-MMMIRAISLGETLVLLGRFELEA----MFKAVEKYKV 296
>At3g48990.1 68416.m05351 AMP-dependent synthetase and ligase family
protein similar to peroxisomal-coenzyme A synthetase
(FAT2) [gi:586339] from Saccharomyces cerevisiae;
contains Pfam AMP-binding enzyme domain PF00501;
identical to cDNA; identical to cDNA adenosine
monophosphate binding protein 3 AMPBP3
(AMPBP3)GI:20799714
Length = 514
Score = 32.7 bits (71), Expect = 1.3
Identities = 41/202 (20%), Positives = 78/202 (38%), Gaps = 7/202 (3%)
Query: 485 LTCAELHKKAERIGNLLLEKGRVNTGDHVALIFPPGLDLICAFYGCLYVGAVPVTIRPPH 544
LT A LH ER + L+ + GD VAL FP ++ + F + A + +
Sbjct: 31 LTHARLHDLIERAASRLVSDAGIKPGDVVALTFPNTVEFVIMFLAVIRARATAAPLNAAY 90
Query: 545 -PQNLHTTL-PTVRMIVDVSKATLVLSNQSVIKLLRSKEASNVLD--SKAWPMTLDTDDM 600
+ L + ++ SK + ++ KL S + +LD S D+D +
Sbjct: 91 TAEEFEFYLSDSDSKLLLTSKEGNAPAQEAASKLKISHVTATLLDAGSDLVLSVADSDSV 150
Query: 601 PKKKLPIMYRAPTAEMLAYLDFSVSTTGMLAGIKMSHAAVTSLCRSMKIACELYPSRHIA 660
++ + + + TT G+ ++ + S +++K +L S
Sbjct: 151 VDSATELVNHPDDGALFLH---TSGTTSRPKGVPLTQLNLASSVKNIKAVYKLTESDSTV 207
Query: 661 LCLDPYCGLGFALWCLSSIYSG 682
+ L + G LSS+ +G
Sbjct: 208 IVLPLFHVHGLLAGLLSSLGAG 229
>At5g38120.1 68418.m04592 4-coumarate--CoA ligase family protein /
4-coumaroyl-CoA synthase family protein similar to 4CL2,
Arabidopsis thaliana [gi:12229665], 4CL1, Nicotiana
tabacum [gi:12229631]; contains Pfam AMP-binding enzyme
domain PF00501
Length = 550
Score = 32.3 bits (70), Expect = 1.8
Identities = 15/40 (37%), Positives = 21/40 (52%)
Query: 830 GKLLPGVKVITANPETKGQCGDSHLGEIWVQSPHNASGYF 869
G L GV+ +P T G + GE+W++ P A GYF
Sbjct: 374 GLLSCGVEARIVDPNTGQVMGLNQTGELWLKGPSIAKGYF 413
>At5g36880.1 68418.m04418 acetyl-CoA synthetase, putative /
acetate-CoA ligase, putative similar to SP|P27550
(Escherichia coli) and gi:8439651 (Homo sapiens);
contains Pfam AMP-binding enzyme domain PF00501
Length = 693
Score = 32.3 bits (70), Expect = 1.8
Identities = 19/56 (33%), Positives = 29/56 (51%), Gaps = 1/56 (1%)
Query: 481 VSKVLTCAELHKKAERIGNLLLEKGRVNTGDHVALIFPPGLDLICAFYGCLYVGAV 536
V LT +EL ++ ++ N L + G V GD V + P ++L A C +GAV
Sbjct: 144 VDASLTYSELLQRVCQLANYLKDNG-VKKGDAVVIYLPMLMELPIAMLACARIGAV 198
>At4g05160.1 68417.m00775 4-coumarate--CoA ligase, putative /
4-coumaroyl-CoA synthase, putative similar to 4CL2
[gi:12229665] from Arabidopsis thaliana, 4CL1
[gi:12229631] from Nicotiana tabacum; contains Pfam
AMP-binding enzyme domain PF00501; acyl-activating
enzyme superfamily; identical to cDNA 4-coumarate-CoA
ligase-like protein (At4g05160) GI:29893226
Length = 544
Score = 32.3 bits (70), Expect = 1.8
Identities = 15/40 (37%), Positives = 21/40 (52%)
Query: 829 SGKLLPGVKVITANPETKGQCGDSHLGEIWVQSPHNASGY 868
+G L PGV+ + ET + GEIWV+ P+ GY
Sbjct: 364 AGMLAPGVEAQIVSVETGKSQPPNQQGEIWVRGPNMMKGY 403
>At2g47830.2 68415.m05970 cation efflux family protein / metal
tolerance protein, putative (MTPc1) member of the cation
diffusion facilitator (CDF) family, or cation efflux
(CE) family, PMID:11500563
Length = 468
Score = 31.9 bits (69), Expect = 2.4
Identities = 47/208 (22%), Positives = 79/208 (37%), Gaps = 11/208 (5%)
Query: 491 HKKAERIGNLLLEKGRVNTGDHVALIFPPGLDLICAFYGCLYVGAVPVTIRPPHPQNLHT 550
H K E +G L + + TG +A LDL+ + + A P I H +
Sbjct: 139 HGKFETLGALGISAMLLATGSGIAW---HALDLLS-----IALSAAPEVIHSGHHHGIDM 190
Query: 551 TLPTVRMIVDVSKATLVLSNQSVIKLLRSKEASNVLDSKAWPMTLDTDDMPKKKLPIMYR 610
P + + V ++ ++ + K K+ S ++ + AW D + +
Sbjct: 191 NHPILALTVTIASISIKEGLYWITKRAGEKQGSGLMMANAWHHRSDAISSLVALVGVGGS 250
Query: 611 APTAEMLAYL-DFSVSTTGMLAGIKMSHAAVTSLCRSMKIACELYPSRHIALCLDPY--C 667
L L VST + AG+K H ++ L + A +L P R L ++ C
Sbjct: 251 ILGVNFLDPLAGLVVSTMIVNAGLKTGHQSILELVDAAIPAQQLEPIRQTILQVEGVKGC 310
Query: 668 GLGFALWCLSSIYSGHHSILIPPSEVEV 695
SS+Y H ++ P S V V
Sbjct: 311 HRLRGRRAGSSLYLDVHIVVDPFSSVSV 338
>At2g47830.1 68415.m05969 cation efflux family protein / metal
tolerance protein, putative (MTPc1) member of the cation
diffusion facilitator (CDF) family, or cation efflux
(CE) family, PMID:11500563
Length = 471
Score = 31.9 bits (69), Expect = 2.4
Identities = 47/208 (22%), Positives = 79/208 (37%), Gaps = 11/208 (5%)
Query: 491 HKKAERIGNLLLEKGRVNTGDHVALIFPPGLDLICAFYGCLYVGAVPVTIRPPHPQNLHT 550
H K E +G L + + TG +A LDL+ + + A P I H +
Sbjct: 139 HGKFETLGALGISAMLLATGSGIAW---HALDLLS-----IALSAAPEVIHSGHHHGIDM 190
Query: 551 TLPTVRMIVDVSKATLVLSNQSVIKLLRSKEASNVLDSKAWPMTLDTDDMPKKKLPIMYR 610
P + + V ++ ++ + K K+ S ++ + AW D + +
Sbjct: 191 NHPILALTVTIASISIKEGLYWITKRAGEKQGSGLMMANAWHHRSDAISSLVALVGVGGS 250
Query: 611 APTAEMLAYL-DFSVSTTGMLAGIKMSHAAVTSLCRSMKIACELYPSRHIALCLDPY--C 667
L L VST + AG+K H ++ L + A +L P R L ++ C
Sbjct: 251 ILGVNFLDPLAGLVVSTMIVNAGLKTGHQSILELVDAAIPAQQLEPIRQTILQVEGVKGC 310
Query: 668 GLGFALWCLSSIYSGHHSILIPPSEVEV 695
SS+Y H ++ P S V V
Sbjct: 311 HRLRGRRAGSSLYLDVHIVVDPFSSVSV 338
>At1g20490.1 68414.m02553 AMP-dependent synthetase and ligase family
protein similar to SP|Q42524 and SP|Q9S725; contains
Pfam AMP-binding enzyme domain PF00501
Length = 447
Score = 31.9 bits (69), Expect = 2.4
Identities = 13/40 (32%), Positives = 22/40 (55%)
Query: 830 GKLLPGVKVITANPETKGQCGDSHLGEIWVQSPHNASGYF 869
G L G++ +P+T G + GE+W++ P + GYF
Sbjct: 373 GLLSSGIEARIVDPDTGRVMGVNQPGELWLKGPSISKGYF 412
>At4g21170.1 68417.m03062 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 534
Score = 31.5 bits (68), Expect = 3.1
Identities = 20/66 (30%), Positives = 29/66 (43%), Gaps = 1/66 (1%)
Query: 688 IPPSEVEVNPALWLSAVSQYKVRDTFCSYGVMELCTKGLGSSVNQLKSKGINLACVRTCV 747
+ PS+ N L S V + + R C Y M LC +G SV +L G+ + T +
Sbjct: 184 LSPSQSAYNSLLG-SLVKENQFRVALCLYSAMILCEQGRSKSVFKLMETGVESCKIYTNL 242
Query: 748 VVAEER 753
V R
Sbjct: 243 VECYSR 248
>At3g47570.1 68416.m05179 leucine-rich repeat transmembrane protein
kinase, putative protein kinase Xa21 - Oryza sativa,
PIR:A57676
Length = 1010
Score = 29.9 bits (64), Expect = 9.5
Identities = 28/90 (31%), Positives = 41/90 (45%), Gaps = 6/90 (6%)
Query: 441 GYTDDSDAARKYQFISQILR-WRAQSTSDHVIFTLLNAKGAVSKVLTCAELHKKAERIGN 499
G+TD++D QF SQ+ R +S + F L N KG +TC +K+ +
Sbjct: 19 GFTDETDRQALLQFKSQVSEDKRVVLSSWNHSFPLCNWKG-----VTCGRKNKRVTHLEL 73
Query: 500 LLLEKGRVNTGDHVALIFPPGLDLICAFYG 529
L+ G V + L F LDL F+G
Sbjct: 74 GRLQLGGVISPSIGNLSFLVSLDLYENFFG 103
>At1g71060.1 68414.m08201 pentatricopeptide (PPR) repeat-containing
protein low similarity to CRP1 [Zea mays] GI:3289002;
contains Pfam profile PF01535: PPR repeat
Length = 510
Score = 29.9 bits (64), Expect = 9.5
Identities = 21/84 (25%), Positives = 41/84 (48%), Gaps = 5/84 (5%)
Query: 573 VIKLLRSKEASNVLDSKAWPMTLDTDDMPKKKLPIMYRAPTAEMLAYLDFSVSTTGMLAG 632
+I++ RSKEA V + + T+ T ++ + + +M + + G+L G
Sbjct: 382 LIRMQRSKEAYEVYQTMSCEPTVSTYEIMVR---MFCNKERLDMAIKIWDEMKGKGVLPG 438
Query: 633 IKMSHAAVTSLCRSMKI--ACELY 654
+ M + +T+LC K+ ACE +
Sbjct: 439 MHMFSSLITALCHENKLDEACEYF 462
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.322 0.135 0.415
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 23,666,448
Number of Sequences: 28952
Number of extensions: 949483
Number of successful extensions: 2058
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 2042
Number of HSP's gapped (non-prelim): 26
length of query: 1071
length of database: 12,070,560
effective HSP length: 89
effective length of query: 982
effective length of database: 9,493,832
effective search space: 9322943024
effective search space used: 9322943024
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 64 (29.9 bits)
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