GeneModel BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA002087-TA|BGIBMGA002087-PA|IPR001398|Macrophage
migration inhibitory factor
         (120 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g01650.1 68418.m00081 macrophage migration inhibitory factor ...    60   3e-10
At5g57170.1 68418.m07141 macrophage migration inhibitory factor ...    57   4e-09
At3g51660.1 68416.m05665 macrophage migration inhibitory factor ...    34   0.022
At3g49500.1 68416.m05410 RNA-dependent RNA polymerase (SDE1) ide...    31   0.27 
At1g44970.1 68414.m05155 peroxidase, putative similar to peroxid...    29   0.63 
At3g48390.1 68416.m05282 MA3 domain-containing protein similar t...    27   2.5  
At1g07990.1 68414.m00871 SIT4 phosphatase-associated family prot...    27   2.5  
At2g47550.1 68415.m05934 pectinesterase family protein contains ...    27   3.4  
At3g20150.1 68416.m02554 kinesin motor family protein contains P...    27   4.4  
At1g13040.1 68414.m01512 pentatricopeptide (PPR) repeat-containi...    27   4.4  
At5g13470.1 68418.m01550 expressed protein                             26   7.8  
At4g00230.1 68417.m00025 subtilisin-like serine endopeptidase (X...    26   7.8  
At2g37040.1 68415.m04544 phenylalanine ammonia-lyase 1 (PAL1) ne...    26   7.8  

>At5g01650.1 68418.m00081 macrophage migration inhibitory factor
           family protein / MIF family protein contains pfam
           profile: PF001187 Macrophage migration inhibitory factor
          Length = 115

 Score = 60.5 bits (140), Expect = 3e-10
 Identities = 36/117 (30%), Positives = 59/117 (50%), Gaps = 4/117 (3%)

Query: 1   MPCLKIFTNLTNSFIP-NDFVNKIIPVLSKAVQKSPEKFICLI-SPDCALSIGGESSTPG 58
           MPCL + TN+    +  +  +++    ++K + K PE ++ ++      +S GG +  P 
Sbjct: 1   MPCLNLSTNVNLDGVDTSSILSEASSTVAKIIGK-PENYVMIVLKGSVPMSFGG-TEDPA 58

Query: 59  AVATLESIGNLGPSQNKVIAKEISDFVEKELGISKDRFFLTFYDLKNFNVAKGGITV 115
           A   L SIG L    NK ++  +S  +E +L + K RFFL FYD K       G T+
Sbjct: 59  AYGELVSIGGLNADVNKKLSAAVSAILETKLSVPKSRFFLKFYDTKGSFFGWNGATL 115


>At5g57170.1 68418.m07141 macrophage migration inhibitory factor
           family protein / MIF family protein contains Pfam
           profile: PF01187 Macrophage migration inhibitory
           factor(MIF)
          Length = 115

 Score = 56.8 bits (131), Expect = 4e-09
 Identities = 30/103 (29%), Positives = 54/103 (52%), Gaps = 2/103 (1%)

Query: 1   MPCLKIFTNL-TNSFIPNDFVNKIIPVLSKAVQKSPEKFICLISPDCALSIGGESSTPGA 59
           MP L +FTN+  ++   +D +      ++K + K     + L++    ++  G +  P A
Sbjct: 1   MPTLNLFTNIPVDAVTCSDILKDATKAVAKIIGKPESYVMILLNSGVPIAFAG-TEEPAA 59

Query: 60  VATLESIGNLGPSQNKVIAKEISDFVEKELGISKDRFFLTFYD 102
              L SIG LGP  N  +++ IS+ ++ +L I   RF++ FYD
Sbjct: 60  YGELISIGGLGPGVNGKLSETISEILQIKLSIDSSRFYIKFYD 102


>At3g51660.1 68416.m05665 macrophage migration inhibitory factor
           family protein / MIF family protein contains Pfam
           profile: PF01187 Macrophage migration inhibitory factor
           family(MIF)
          Length = 112

 Score = 34.3 bits (75), Expect = 0.022
 Identities = 20/106 (18%), Positives = 44/106 (41%), Gaps = 2/106 (1%)

Query: 1   MPCLKIFTNLTNSFIPND-FVNKIIPVLSKAVQKSPEKFICLISPDCALSIGGESSTPGA 59
           MPCL I TN+    +  D F +++   ++  V + P+  + ++       + G +    A
Sbjct: 1   MPCLYITTNVNFDGVNTDPFYSEVTKAVASIVGR-PQNLVMVVLKGSVEIVFGGNKEAAA 59

Query: 60  VATLESIGNLGPSQNKVIAKEISDFVEKELGISKDRFFLTFYDLKN 105
            A + S+G +     + +   +   +     I   RF    +D+ +
Sbjct: 60  YAEIVSMGGITKQVKRELIATVGSILHTHFSIHPTRFIFKVFDINS 105


>At3g49500.1 68416.m05410 RNA-dependent RNA polymerase (SDE1)
          identical to RNA-dependent RNA polymerase [Arabidopsis
          thaliana] gi|8248473|gb|AAF74208
          Length = 1196

 Score = 30.7 bits (66), Expect = 0.27
 Identities = 16/42 (38%), Positives = 25/42 (59%), Gaps = 3/42 (7%)

Query: 50 IGGESSTPGAVATLESIGNLGPSQNKVIAKEISDFVEKELGI 91
          +G E +   +V T  SIG  G S     AK+++D++E E+GI
Sbjct: 1  MGSEGNMKKSVVTQVSIGGFGESTT---AKQLTDYLEDEVGI 39


>At1g44970.1 68414.m05155 peroxidase, putative similar to peroxidase
           GI:993004 from [Mercurialis annua]
          Length = 346

 Score = 29.5 bits (63), Expect = 0.63
 Identities = 20/70 (28%), Positives = 33/70 (47%), Gaps = 3/70 (4%)

Query: 23  IIPVLSKAVQKSPEKFICLIS---PDCALSIGGESSTPGAVATLESIGNLGPSQNKVIAK 79
           ++ VL KA+ K P     L+     DC +     S      AT+ S  N GP++N V   
Sbjct: 61  VMTVLEKAIAKEPRMAASLLRLHFHDCFVQGCDASILLDDSATIRSEKNAGPNKNSVRGF 120

Query: 80  EISDFVEKEL 89
           ++ D ++ +L
Sbjct: 121 QVIDEIKAKL 130


>At3g48390.1 68416.m05282 MA3 domain-containing protein similar to
           programmed cell death 4 protein [Gallus gallus]
           GI:12958564; contains Pfam profile PF02847: MA3 domain
          Length = 633

 Score = 27.5 bits (58), Expect = 2.5
 Identities = 20/74 (27%), Positives = 34/74 (45%), Gaps = 3/74 (4%)

Query: 19  FVNKIIPVLSKAVQKSPEKFICLISPDCALSIGGESSTPGAVATLESIGNLG---PSQNK 75
           FV +++ +      K  EK   L+S   AL +  +    G +  LES+G+L    P    
Sbjct: 93  FVKRLVSMAMDRGNKEKEKASVLLSRLYALVVSPDQIRVGFIRLLESVGDLALDIPDAVN 152

Query: 76  VIAKEISDFVEKEL 89
           V+A  I+  +  E+
Sbjct: 153 VLALFIARAIVDEI 166


>At1g07990.1 68414.m00871 SIT4 phosphatase-associated family protein
           contains Pfam profile: PF04499 SIT4
           phosphatase-associated protein
          Length = 802

 Score = 27.5 bits (58), Expect = 2.5
 Identities = 16/60 (26%), Positives = 30/60 (50%)

Query: 4   LKIFTNLTNSFIPNDFVNKIIPVLSKAVQKSPEKFICLISPDCALSIGGESSTPGAVATL 63
           L +   L +S +    V+K+ P     VQ +  + +C IS +   ++  + S+PG VA +
Sbjct: 182 LDVMQWLADSNLLEMIVDKLKPSSPPEVQANAAETLCAISRNAPSALATQLSSPGYVAKI 241


>At2g47550.1 68415.m05934 pectinesterase family protein contains
          Pfam profile: PF01095 pectinesterase
          Length = 560

 Score = 27.1 bits (57), Expect = 3.4
 Identities = 22/84 (26%), Positives = 36/84 (42%), Gaps = 6/84 (7%)

Query: 6  IFTNLTNSFIPNDFVNKIIPVLSKAVQKSPEKFICLISPDCALSIGGESSTPGAVATLES 65
          +FT    S +P+ F++  IP +  A   SP   IC  +PD +      S  P     + S
Sbjct: 8  LFTLACLSSLPSPFISAQIPAIGNAT--SPSN-ICRFAPDPSYC---RSVLPNQPGDIYS 61

Query: 66 IGNLGPSQNKVIAKEISDFVEKEL 89
           G L   ++   A+     ++ EL
Sbjct: 62 YGRLSLRRSLSRARRFISMIDAEL 85


>At3g20150.1 68416.m02554 kinesin motor family protein contains Pfam
           domain, PF00225: Kinesin motor domain
          Length = 1114

 Score = 26.6 bits (56), Expect = 4.4
 Identities = 19/80 (23%), Positives = 37/80 (46%), Gaps = 1/80 (1%)

Query: 17  NDFVNKIIPVLSKAVQKSPEKFICLISPDCALSIGGESSTPGAVATLESIGNLGPSQNKV 76
           N+   + +  LS  ++   E+ +  +  D   S+G ++   GA    ES+  L  S N+ 
Sbjct: 443 NEISEEDVNDLSDQIRLLKEE-LSKVKADACHSVGSKNDYFGAKNARESLNQLRVSLNRS 501

Query: 77  IAKEISDFVEKELGISKDRF 96
           +     D  E+E+ + +D F
Sbjct: 502 LMLPKIDNDEEEITVDEDDF 521


>At1g13040.1 68414.m01512 pentatricopeptide (PPR) repeat-containing
           protein contains Pfam profile PF01535: PPR repeat
          Length = 517

 Score = 26.6 bits (56), Expect = 4.4
 Identities = 20/50 (40%), Positives = 30/50 (60%), Gaps = 5/50 (10%)

Query: 74  NKVIAKEISDFVEKELGISKDR-FFLTFYD--LKNFNVAKG-GITVDVLE 119
           N  +AK++ D +  ELG+S DR F+ T  D   K+ NV K  G+  D++E
Sbjct: 375 NSSVAKKLLDQMT-ELGLSPDRIFYTTILDHLCKSGNVDKAYGVFNDMIE 423


>At5g13470.1 68418.m01550 expressed protein
          Length = 159

 Score = 25.8 bits (54), Expect = 7.8
 Identities = 13/37 (35%), Positives = 21/37 (56%)

Query: 29 KAVQKSPEKFICLISPDCALSIGGESSTPGAVATLES 65
          ++V+K P+  + L SP  A   GG+ ++  A A  ES
Sbjct: 48 ESVEKLPDALLLLESPTLAQVTGGDHASVVAAAMAES 84


>At4g00230.1 68417.m00025 subtilisin-like serine endopeptidase
           (XSP1) identical to subtilisin-type serine endopeptidase
           XSP1 GI:6708179 from [Arabidopsis thaliana]
          Length = 749

 Score = 25.8 bits (54), Expect = 7.8
 Identities = 21/74 (28%), Positives = 32/74 (43%), Gaps = 3/74 (4%)

Query: 47  ALSIGGESSTPGAVATLESIGNLGPSQNKVIAKEISDFVEKELGISKDRFFLTFYDLKNF 106
           ++S+G   +    + T+ S GN GPS   V   E         GI  DR F +  DL N 
Sbjct: 292 SISVGSFHAMRKGILTVASAGNDGPSSGTVTNHEPWILTVAASGI--DRTFKSKIDLGN- 348

Query: 107 NVAKGGITVDVLEP 120
             +  G+ + +  P
Sbjct: 349 GKSFSGMGISMFSP 362


>At2g37040.1 68415.m04544 phenylalanine ammonia-lyase 1 (PAL1)
          nearly identical to SP|P35510
          Length = 725

 Score = 25.8 bits (54), Expect = 7.8
 Identities = 10/21 (47%), Positives = 16/21 (76%)

Query: 48 LSIGGESSTPGAVATLESIGN 68
          +++GGE+ T G VA + +IGN
Sbjct: 65 VNLGGETLTIGQVAAISTIGN 85


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.318    0.138    0.397 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,737,624
Number of Sequences: 28952
Number of extensions: 101189
Number of successful extensions: 299
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 8
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 291
Number of HSP's gapped (non-prelim): 13
length of query: 120
length of database: 12,070,560
effective HSP length: 73
effective length of query: 47
effective length of database: 9,957,064
effective search space: 467982008
effective search space used: 467982008
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 54 (25.8 bits)