Silkworm BLAST Result against bee

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA002069-TA|BGIBMGA002069-PA|IPR001452|Src homology-3,
IPR000980|SH2 motif, IPR001245|Tyrosine protein kinase,
IPR011009|Protein kinase-like, IPR000719|Protein kinase,
IPR008266|Tyrosine protein kinase, active site, IPR001720|PI3 kinase,
P85 regulatory subunit
         (473 letters)

Database: bee 
           429 sequences; 140,377 total letters

Searching.....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

AY921579-1|AAX14899.1|  996|Apis mellifera ephrin receptor protein.   180   3e-47
AF469010-1|AAL93136.1|  678|Apis mellifera cGMP-dependent protei...    61   2e-11
AB013288-1|BAA87894.1|  149|Apis mellifera protein kinase C prot...    57   4e-10
AB183889-1|BAD86829.1|  316|Apis mellifera Mos protein.                47   4e-07
DQ013068-1|AAY81956.1|  931|Apis mellifera dusty protein kinase ...    45   1e-06
DQ013067-1|AAY81955.1|  969|Apis mellifera dusty protein kinase ...    45   1e-06
AB013287-1|BAA87893.1|  190|Apis mellifera calmodulin kinase II ...    45   1e-06

>AY921579-1|AAX14899.1|  996|Apis mellifera ephrin receptor protein.
          Length = 996

 Score =  180 bits (437), Expect = 3e-47
 Identities = 101/229 (44%), Positives = 136/229 (59%), Gaps = 11/229 (4%)

Query: 244 EIDRTDIVMKHKLGGGQYGDVYEAAWK-----RGNITVAVKTLKDDTM--ALKDFLEEAS 296
           EID   I ++  +GGG++GDV     K     R  I VA+KTLK  +   A  DFL EAS
Sbjct: 627 EIDAGYITIEAIIGGGEFGDVCRGKLKLPPDGRTEIDVAIKTLKPGSADKARNDFLTEAS 686

Query: 297 IMKEMRHPNLVQLLGVCTREPPFYIITEFMSRGNLLEYLRAGARECVPGAVVLMYMATQI 356
           IM +  HPN++ L GV T+  P  IITEFM  G+L  +LRA   +     + L+ M   I
Sbjct: 687 IMGQFEHPNVIFLQGVVTKSNPVMIITEFMENGSLDTFLRANDGKFQ--VLQLVGMLRGI 744

Query: 357 ASGMSYLESRSFIHRDLAARNCLVGENHLVKVADFGLARLMRDDTYTAHA--GAKFPIKW 414
           ASGM YL   +++HRDLAARN LV    + K+ADFGL+R +   T  A+   G K P++W
Sbjct: 745 ASGMQYLAEMNYVHRDLAARNVLVNAALVCKIADFGLSREIESATEGAYTTRGGKIPVRW 804

Query: 415 TAPEGLAYNTFSTKSDVWAFGILLWEIATYGMSPYPGVDLADVYHMLEK 463
           TAPE +A+  F++ SDVW+ GI+ WE+ +YG  PY      DV   +EK
Sbjct: 805 TAPEAIAFRKFTSASDVWSMGIVCWEVMSYGERPYWNWSNQDVIKSIEK 853



 Score = 23.0 bits (47), Expect = 5.5
 Identities = 11/38 (28%), Positives = 16/38 (42%)

Query: 308 QLLGVCTREPPFYIITEFMSRGNLLEYLRAGARECVPG 345
           Q +G C           F+ +G+   YL +G   C PG
Sbjct: 215 QTIGTCVANAVVIEQPTFLCKGDGKWYLPSGGCHCKPG 252


>AF469010-1|AAL93136.1|  678|Apis mellifera cGMP-dependent protein
           kinase foraging protein.
          Length = 678

 Score = 60.9 bits (141), Expect = 2e-11
 Identities = 48/181 (26%), Positives = 75/181 (41%), Gaps = 8/181 (4%)

Query: 284 DTMALKDFLEEASIMKEMRHPNLVQLLGVCTREPPFYIITEFMSRGNLLEYLRAGARECV 343
           +T   +  + E  IM E     +V+L          Y++ E    G L   LR       
Sbjct: 406 ETRQQQHIMSEKRIMGEADCDFVVKLFKTFKDRKYLYMLMEACLGGELWTVLRDKGH--F 463

Query: 344 PGAVVLMYMATQIASGMSYLESRSFIHRDLAARNCLVGENHLVKVADFGLARLMRDDTYT 403
                  Y A  +     YL SR+ I+RDL   N L+     VK+ DFG A+ +     T
Sbjct: 464 DDGTTRFYTAC-VVEAFDYLHSRNIIYRDLKPENLLLDSQGYVKLVDFGFAKRLDHGRKT 522

Query: 404 -AHAGAKFPIKWTAPEGLAYNTFSTKSDVWAFGILLWEIATYGMSPYPGVDLADVYHMLE 462
               G     ++ APE +        +D W+ G+L++E+ T G  P+ G D    Y+++ 
Sbjct: 523 WTFCGTP---EYVAPEVILNKGHDISADYWSLGVLMFELLT-GTPPFTGGDPMKTYNIIL 578

Query: 463 K 463
           K
Sbjct: 579 K 579


>AB013288-1|BAA87894.1|  149|Apis mellifera protein kinase C
           protein.
          Length = 149

 Score = 56.8 bits (131), Expect = 4e-10
 Identities = 36/124 (29%), Positives = 65/124 (52%), Gaps = 8/124 (6%)

Query: 277 AVKTLKDDTMALKDFLEEASIMKEM-----RHPNLVQLLGVCTREPPFYIITEFMSRGNL 331
           A+K LK D +   D +E   + K +     + P LVQL          Y + E+++ G+L
Sbjct: 13  AIKILKKDIIIQDDDVECTMVEKRVLALSTKPPFLVQLHSCFQTMDRLYFVMEYVNGGDL 72

Query: 332 LEYLRAGARECVPGAVVLMYMATQIASGMSYLESRSFIHRDLAARNCLVGENHLVKVADF 391
           +  ++   +   P AV   + A++IA G+ +L  R  ++RDL   N L+ ++  +K+ADF
Sbjct: 73  MYQIQQCGKFKEPVAV---FYASEIAIGLFFLHGRGIVYRDLKLDNVLLDQDGHIKIADF 129

Query: 392 GLAR 395
           G+ +
Sbjct: 130 GMCK 133


>AB183889-1|BAD86829.1|  316|Apis mellifera Mos protein.
          Length = 316

 Score = 46.8 bits (106), Expect = 4e-07
 Identities = 46/209 (22%), Positives = 92/209 (44%), Gaps = 14/209 (6%)

Query: 256 LGGGQYGDVYEAAWKRGNITVAVKTLKDDTMALKDFLEEASIMKEMRHPNLVQLLGVCTR 315
           LG G +G VY+A +K     VA K ++  T    + L        ++H N+V++L +  +
Sbjct: 73  LGSGGFGIVYKALYKGEQ--VAAKIIQ--TEKYSNMLNSEKHASFLKHSNIVKVLMI-EQ 127

Query: 316 EPPFYIITEFMSRGNLLEYLRAGARECVPGAVVLMYMATQIASGMSYLESRSFIHRDLAA 375
                +IT  +    L   L     E +      + +   I   + +  +   +H D+  
Sbjct: 128 GASLSLITMELCGTTLQNRLD----EAILIKNERICILKSITCALQFCHNAGIVHADVKP 183

Query: 376 RNCLVGENHLVKVADFGLARLM-RDDTYTAHAGAKFPIKWTAPEGLAYNTFSTKSDVWAF 434
           +N L+ +N   K+ DFG + L+   +      G      +TAPE +  N  +  +D+++ 
Sbjct: 184 KNILMSKNGQPKLTDFGSSVLIGAPNEIDKFYGTP---GYTAPEVIKQNRPTPAADIYSL 240

Query: 435 GILLWEIATYGMSPYPGVDLADVYHMLEK 463
           GI+ W++  +   P+ G+    + ++  K
Sbjct: 241 GIVAWQM-LFRKLPFAGLHSHTIIYLSAK 268


>DQ013068-1|AAY81956.1|  931|Apis mellifera dusty protein kinase
           isoform B protein.
          Length = 931

 Score = 45.2 bits (102), Expect = 1e-06
 Identities = 30/99 (30%), Positives = 48/99 (48%), Gaps = 6/99 (6%)

Query: 352 MATQIASGMSYLESRSFIHRDLAARNCLVGENHLVKVADFGLARLMRDDTYTAHAGAKFP 411
           +A  +  G+ YL S+  +HRD+  +N L+   +  K+ DFG       +     +    P
Sbjct: 702 IALDVLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGFC---ITEVMMLGSIVGTP 758

Query: 412 IKWTAPEGLAYNTFSTKSDVWAFGILLWEI-ATYGMSPY 449
           +   APE L    + +  DV+AFGIL W + A +   PY
Sbjct: 759 VH-MAPE-LLSGHYDSSVDVYAFGILFWYLCAGHVRLPY 795


>DQ013067-1|AAY81955.1|  969|Apis mellifera dusty protein kinase
           isoform A protein.
          Length = 969

 Score = 45.2 bits (102), Expect = 1e-06
 Identities = 30/99 (30%), Positives = 48/99 (48%), Gaps = 6/99 (6%)

Query: 352 MATQIASGMSYLESRSFIHRDLAARNCLVGENHLVKVADFGLARLMRDDTYTAHAGAKFP 411
           +A  +  G+ YL S+  +HRD+  +N L+   +  K+ DFG       +     +    P
Sbjct: 740 IALDVLEGIRYLHSQGLVHRDVKLKNVLLDIENRAKLTDFGFC---ITEVMMLGSIVGTP 796

Query: 412 IKWTAPEGLAYNTFSTKSDVWAFGILLWEI-ATYGMSPY 449
           +   APE L    + +  DV+AFGIL W + A +   PY
Sbjct: 797 VH-MAPE-LLSGHYDSSVDVYAFGILFWYLCAGHVRLPY 833


>AB013287-1|BAA87893.1|  190|Apis mellifera calmodulin kinase II
           protein.
          Length = 190

 Score = 45.2 bits (102), Expect = 1e-06
 Identities = 31/111 (27%), Positives = 51/111 (45%), Gaps = 5/111 (4%)

Query: 355 QIASGMSYLESRSFIHRDLAARNCLVG---ENHLVKVADFGLARLMRDDTYTAHAGAKFP 411
           QI   + +      +HRDL   N L+    +   VK+ADFGLA  ++ +       A  P
Sbjct: 17  QILESVHHCHHNGVVHRDLKPENLLLASKAKGAAVKLADFGLAIEVQGEAQAWFGFAGTP 76

Query: 412 IKWTAPEGLAYNTFSTKSDVWAFGILLWEIATYGMSPYPGVDLADVYHMLE 462
             + +PE L    +    D+WA G++L+ I   G  P+   D   +Y  ++
Sbjct: 77  -GYLSPEVLKKEPYGKPVDIWACGVILY-ILLVGYPPFWDEDQHRLYAQIK 125


  Database: bee
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 140,377
  Number of sequences in database:  429
  
Lambda     K      H
   0.320    0.135    0.416 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 131,482
Number of Sequences: 429
Number of extensions: 5574
Number of successful extensions: 21
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 4
Number of HSP's gapped (non-prelim): 9
length of query: 473
length of database: 140,377
effective HSP length: 60
effective length of query: 413
effective length of database: 114,637
effective search space: 47345081
effective search space used: 47345081
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 45 (22.2 bits)