Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA002052-TA|BGIBMGA002052-PA|IPR011765|Peptidase M16,
N-terminal, IPR007863|Peptidase M16, C-terminal
(466 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g02090.1 68416.m00174 mitochondrial processing peptidase beta... 310 2e-84
At3g02090.2 68416.m00175 mitochondrial processing peptidase beta... 281 9e-76
At1g51980.1 68414.m05863 mitochondrial processing peptidase alph... 147 2e-35
At3g16480.1 68416.m02103 mitochondrial processing peptidase alph... 134 1e-31
At5g56730.1 68418.m07080 peptidase M16 family protein / insulina... 48 1e-05
At5g42390.1 68418.m05161 metalloendopeptidase identical to chlor... 41 0.002
At3g13170.1 68416.m01647 DNA topoisomerase VIA (SPO11-1) identic... 31 1.6
At1g06900.1 68414.m00733 peptidase M16 family protein / insulina... 30 2.8
At3g11490.1 68416.m01401 rac GTPase activating protein, putative... 29 6.6
>At3g02090.1 68416.m00174 mitochondrial processing peptidase beta
subunit, putative similar to mitochondrial processing
peptidase beta subunit, mitochondrial precursor,
Beta-MPP [Human] SWISS-PROT:O75439
Length = 531
Score = 310 bits (760), Expect = 2e-84
Identities = 160/450 (35%), Positives = 255/450 (56%), Gaps = 7/450 (1%)
Query: 23 PYPI-PFIDFLKNIPDTQYSRLDNGLTIATEERESYNTC-VGLFIDAGSRYEDNFENGVG 80
P+PI + + + P+T+ + L NGL +ATE S T VG++IDAGSR+E + NG
Sbjct: 81 PHPILASHNHILSAPETRVTTLPNGLRVATESNLSAKTATVGVWIDAGSRFESDETNGTA 140
Query: 81 HFFEHLAFKGTKCRTKTLLEDQISSSGAIFKCFTTREMVAYYADCLSYDLPRVVEILTDC 140
HF EH+ FKGT RT LE++I G +T+RE YYA L ++ + +++L D
Sbjct: 141 HFLEHMIFKGTDRRTVRALEEEIEDIGGHLNAYTSREQTTYYAKVLDSNVNQALDVLADI 200
Query: 141 IYNNNFATADIELQRKIVYAEMIEQDKNSNTVLYDYLHSTAFQGTPLAQTVMGPSCNLYN 200
+ N+ F I +R ++ EM E + ++ V+ D+LH+TAFQ TPL +T++GP+ N+ +
Sbjct: 201 LQNSKFEEQRINRERDVILREMQEVEGQTDEVVLDHLHATAFQYTPLGRTILGPAQNVKS 260
Query: 201 FTDMTISRYLAKRFDPARTVLVAVGGVKHDQMVFLANNYLNKL-----TPLKCVDIGVAR 255
T + Y+ + +R V+ A G VKH+++V KL T + V A
Sbjct: 261 ITREDLQNYIKTHYTASRMVIAAAGAVKHEEVVEQVKKLFTKLSSDPTTTSQLVANEPAS 320
Query: 256 YTGSEIRFRNDSLPVANCVMVIEGPSFCHKDQIVMEVAASIISGWDKSQPGGINHAVRIA 315
+TGSE+R +D LP+A + EG S+ D + + V +++ W+K+ GG + +
Sbjct: 321 FTGSEVRMIDDDLPLAQFAVAFEGASWTDPDSVALMVMQTMLGSWNKNVGGGKHVGSDLT 380
Query: 316 REASTDKFCDSYKAVNITYKDTSLWGVQFMGPSVELEDMVLSIQGEWMNMCHTITDAEVE 375
+ + ++ +S A N YKDT L+GV + + L+D+ +I E + + ++DA+V
Sbjct: 381 QRVAINEIAESIMAFNTNYKDTGLFGVYAVAKADCLDDLSYAIMYEVTKLAYRVSDADVT 440
Query: 376 RAKRELKTKVLSKTESCAGTCHEIGRWVLYNGRRPPLHERICAIDSVFAQDIRRVCNKYI 435
RA+ +LK+ +L + + +IGR +L GRR P E ID+V A ++RV NKYI
Sbjct: 441 RARNQLKSSLLLHMDGTSPIAEDIGRQLLTYGRRIPTAELFARIDAVDASTVKRVANKYI 500
Query: 436 YDKCPAVAAVGPTEGLPDYTKIRAGMYWLR 465
YDK A++A+GP + LPDY K R YW R
Sbjct: 501 YDKDIAISAIGPIQDLPDYNKFRRRTYWNR 530
>At3g02090.2 68416.m00175 mitochondrial processing peptidase beta
subunit, putative similar to mitochondrial processing
peptidase beta subunit, mitochondrial precursor,
Beta-MPP [Human] SWISS-PROT:O75439
Length = 535
Score = 281 bits (688), Expect = 9e-76
Identities = 147/423 (34%), Positives = 238/423 (56%), Gaps = 7/423 (1%)
Query: 23 PYPI-PFIDFLKNIPDTQYSRLDNGLTIATEERESYNTC-VGLFIDAGSRYEDNFENGVG 80
P+PI + + + P+T+ + L NGL +ATE S T VG++IDAGSR+E + NG
Sbjct: 81 PHPILASHNHILSAPETRVTTLPNGLRVATESNLSAKTATVGVWIDAGSRFESDETNGTA 140
Query: 81 HFFEHLAFKGTKCRTKTLLEDQISSSGAIFKCFTTREMVAYYADCLSYDLPRVVEILTDC 140
HF EH+ FKGT RT LE++I G +T+RE YYA L ++ + +++L D
Sbjct: 141 HFLEHMIFKGTDRRTVRALEEEIEDIGGHLNAYTSREQTTYYAKVLDSNVNQALDVLADI 200
Query: 141 IYNNNFATADIELQRKIVYAEMIEQDKNSNTVLYDYLHSTAFQGTPLAQTVMGPSCNLYN 200
+ N+ F I +R ++ EM E + ++ V+ D+LH+TAFQ TPL +T++GP+ N+ +
Sbjct: 201 LQNSKFEEQRINRERDVILREMQEVEGQTDEVVLDHLHATAFQYTPLGRTILGPAQNVKS 260
Query: 201 FTDMTISRYLAKRFDPARTVLVAVGGVKHDQMVFLANNYLNKL-----TPLKCVDIGVAR 255
T + Y+ + +R V+ A G VKH+++V KL T + V A
Sbjct: 261 ITREDLQNYIKTHYTASRMVIAAAGAVKHEEVVEQVKKLFTKLSSDPTTTSQLVANEPAS 320
Query: 256 YTGSEIRFRNDSLPVANCVMVIEGPSFCHKDQIVMEVAASIISGWDKSQPGGINHAVRIA 315
+TGSE+R +D LP+A + EG S+ D + + V +++ W+K+ GG + +
Sbjct: 321 FTGSEVRMIDDDLPLAQFAVAFEGASWTDPDSVALMVMQTMLGSWNKNVGGGKHVGSDLT 380
Query: 316 REASTDKFCDSYKAVNITYKDTSLWGVQFMGPSVELEDMVLSIQGEWMNMCHTITDAEVE 375
+ + ++ +S A N YKDT L+GV + + L+D+ +I E + + ++DA+V
Sbjct: 381 QRVAINEIAESIMAFNTNYKDTGLFGVYAVAKADCLDDLSYAIMYEVTKLAYRVSDADVT 440
Query: 376 RAKRELKTKVLSKTESCAGTCHEIGRWVLYNGRRPPLHERICAIDSVFAQDIRRVCNKYI 435
RA+ +LK+ +L + + +IGR +L GRR P E ID+V A ++RV NKYI
Sbjct: 441 RARNQLKSSLLLHMDGTSPIAEDIGRQLLTYGRRIPTAELFARIDAVDASTVKRVANKYI 500
Query: 436 YDK 438
YDK
Sbjct: 501 YDK 503
>At1g51980.1 68414.m05863 mitochondrial processing peptidase alpha
subunit, putative similar to mitochondrial processing
peptidase alpha subunit, mitochondrial precursor,
Alpha-MPP (Ubiquinol-cytochrome C reductase subunit II)
[Potato] SWISS-PROT:P29677
Length = 503
Score = 147 bits (356), Expect = 2e-35
Identities = 117/451 (25%), Positives = 201/451 (44%), Gaps = 22/451 (4%)
Query: 18 PVRNRPYPIPFIDFLKNIPDTQYSRLDNGLTIATEERESYNTCVGLFIDAGSRYEDNFEN 77
P++ P P D ++ Q + L NGL IA+E + +GL++D GS YE + +
Sbjct: 59 PLQGVSLPPPLADKVEP-SKLQITTLPNGLKIASETTPNPAASIGLYVDCGSIYEAPYFH 117
Query: 78 GVGHFFEHLAFKGTKCRTKTLLEDQISSSGAIFKCFTTREMVAYYADCLSYDLPRVVEIL 137
G H E +AFK T RT L +I + G +RE ++Y D L +P +VE+L
Sbjct: 118 GATHLLERMAFKSTLNRTHFRLVREIEAIGGNTSASASREQMSYTIDALKTYVPEMVEVL 177
Query: 138 TDCIYNNNFATADIELQRKIVYAEMIEQDKNSNTVLYDYLHSTAFQGTPLAQTVMGPSCN 197
D + N F ++ + + + E+ E KN L + +HS + G PLA + P
Sbjct: 178 IDSVRNPAFLDWEVNEELRKMKVEIAELAKNPMGFLLEAIHSAGYSG-PLASPLYAPESA 236
Query: 198 LYNFTDMTISRYLAKRFDPARTVLVAVGGVKHDQMVFLANNYLNKLTPLKCVDIGVARYT 257
L + ++ + F AR VL A GV+H++++ +A + L + ++Y
Sbjct: 237 LDRLNGELLEEFMTENFTAARMVL-AASGVEHEELLKVAEPLTSDLPNVPPQLAPKSQYV 295
Query: 258 GSEIRFRNDSLPVANCVMVIEGPSFCHKDQIVMEVAASIISG----WDKSQPGGINHAVR 313
G + R ++ + + E P + ++ + V ++ G + PG H+
Sbjct: 296 GGDFR-QHTGGEATHFAVAFEVPGWNNEKEAVTATVLQMLMGGGGSFSAGGPGKGMHSWL 354
Query: 314 IAREASTDKFCDSYKAVNITYKDTSLWGV------QFMGPSVELEDMVL-SIQGEWMNMC 366
R + + S A + DT L+G+ QF ++EL L + G +N
Sbjct: 355 YRRVLNEYQEVQSCTAFTSIFNDTGLFGIYGCSSPQFAAKAIELAAKELKDVAGGKVNQA 414
Query: 367 HTITDAEVERAKRELKTKVLSKTESCAGTCHEIGRWVLYNGRRPPLHERICAIDSVFAQD 426
H ++RAK K+ VL ES +IGR +L G R P+ + + ++D + +D
Sbjct: 415 H------LDRAKAATKSAVLMNLESRMIAAEDIGRQILTYGERKPVDQFLKSVDQLTLKD 468
Query: 427 IRRVCNKYIYDKCPAVAAVGPTEGLPDYTKI 457
I +K I K + + G +P Y I
Sbjct: 469 IADFTSKVI-SKPLTMGSFGDVLAVPSYDTI 498
>At3g16480.1 68416.m02103 mitochondrial processing peptidase alpha
subunit, putative similar to mitochondrial processing
peptidase alpha subunit, mitochondrial precursor,
Alpha-MPP (Ubiquinol-cytochrome C reductase subunit II)
[Potato] SWISS-PROT:P29677
Length = 499
Score = 134 bits (324), Expect = 1e-31
Identities = 110/446 (24%), Positives = 193/446 (43%), Gaps = 12/446 (2%)
Query: 18 PVRNRPYPIPFIDFLKNIPDTQYSRLDNGLTIATEERESYNTCVGLFIDAGSRYEDNFEN 77
P+ P P D ++ + + L NGLTIATE + +GL++D GS YE
Sbjct: 55 PLAGVSLPPPLSDHVEP-SKLKTTTLPNGLTIATEMSPNPAASIGLYVDCGSIYETPQFR 113
Query: 78 GVGHFFEHLAFKGTKCRTKTLLEDQISSSGAIFKCFTTREMVAYYADCLSYDLPRVVEIL 137
G H E +AFK T R+ L +I + G +RE + Y D L +P +VE+L
Sbjct: 114 GATHLLERMAFKSTLNRSHFRLVREIEAIGGNTSASASREQMGYTIDALKTYVPEMVEVL 173
Query: 138 TDCIYNNNFATADIELQRKIVYAEMIEQDKNSNTVLYDYLHSTAFQGTPLAQTVMGPSCN 197
D + N F ++ + + V E+ E N L + +HS + G LA + P
Sbjct: 174 IDSVRNPAFLDWEVNEELRKVKVEIGEFATNPMGFLLEAVHSAGYSGA-LANPLYAPESA 232
Query: 198 LYNFTDMTISRYLAKRFDPARTVLVAVGGVKHDQMVFLANNYLNKLTPLKCVDIGVARYT 257
+ T + ++ + + +R VL A GV H++++ + L+ L + ++Y
Sbjct: 233 ITGLTGEVLENFVFENYTASRMVL-AASGVDHEELLKVVEPLLSDLPNVPRPAEPKSQYV 291
Query: 258 GSEIRFRNDSLPVANCVMVIEGPSFCH-KDQIVMEVAASIISG---WDKSQPGGINHAVR 313
G + R ++ + + E P + + K+ I+ V ++ G + PG H+
Sbjct: 292 GGDFR-QHTGGEATHFALAFEVPGWNNEKEAIIATVLQMLMGGGGSFSAGGPGKGMHSWL 350
Query: 314 IAREASTDKFCDSYKAVNITYKDTSLWGVQFMGPSVELEDMVLSIQGEWMNMC--HTITD 371
R + + S A + +T L+G+ + S E + + MN +
Sbjct: 351 YLRLLNQHQQFQSCTAFTSVFNNTGLFGI-YGCTSPEFASQGIELVASEMNAVADGKVNQ 409
Query: 372 AEVERAKRELKTKVLSKTESCAGTCHEIGRWVLYNGRRPPLHERICAIDSVFAQDIRRVC 431
++RAK K+ +L ES +IGR +L G R P+ + + +D + +DI
Sbjct: 410 KHLDRAKAATKSAILMNLESRMIAAEDIGRQILTYGERKPVDQFLKTVDQLTLKDIADFT 469
Query: 432 NKYIYDKCPAVAAVGPTEGLPDYTKI 457
+K I K +A G +P Y +
Sbjct: 470 SKVI-TKPLTMATFGDVLNVPSYDSV 494
>At5g56730.1 68418.m07080 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain
Length = 956
Score = 48.4 bits (110), Expect = 1e-05
Identities = 48/197 (24%), Positives = 80/197 (40%), Gaps = 13/197 (6%)
Query: 40 YSRLDNGLT--IATEERESYNTCVGLFIDAGSRYEDNFENGVGHFFEHLAFKGTKCRTKT 97
Y RLDNGL + + + L + GS E+ + GV H EHLAF T T
Sbjct: 42 YGRLDNGLIYYVRRNSKPRMRAALALAVKVGSVLEEEDQRGVAHIVEHLAFSATTRYTNH 101
Query: 98 LLEDQISSSGAIF----KCFTTREMVAYYADCLSYDLP----RVVEILTDCIYNNNFATA 149
+ + S GA F TT + Y + D P + + IL + +
Sbjct: 102 DIVKFLESIGAEFGPCQNAMTTADETIYEL-FVPVDKPELLSQAISILAEFSSEIRVSKE 160
Query: 150 DIELQRKIVYAEMIEQDKNSNTVLYDYLHSTAFQGTPLAQTV-MGPSCNLYNFTDMTISR 208
D+E +R V E ++N+ + D +G+ A+ + +G + + T+ +
Sbjct: 161 DLEKERGAVMEE-YRGNRNATGRMQDSHWQLMMEGSKYAERLPIGLEKVIRSVPAATVKQ 219
Query: 209 YLAKRFDPARTVLVAVG 225
+ K + +VAVG
Sbjct: 220 FYQKWYHLCNMAVVAVG 236
>At5g42390.1 68418.m05161 metalloendopeptidase identical to
chloroplast processing enzyme metalloendopeptidase
[Arabidopsis thaliana] gi|2827039|gb|AAC39482
Length = 1265
Score = 41.1 bits (92), Expect = 0.002
Identities = 26/99 (26%), Positives = 47/99 (47%), Gaps = 1/99 (1%)
Query: 65 IDAGSRYEDNFENGVGHFFEHLAFKGTKCRTKTLLEDQISSSGAIFKCFTTREMVAYYAD 124
+ GS E+ E G+ H EH+AF G+K R K L S++ F +
Sbjct: 224 VHVGSIDEEEDEQGIAHMIEHVAFLGSKKREKLLGTGARSNAYTDFHHTVFHIHSPTHTK 283
Query: 125 CLSYDL-PRVVEILTDCIYNNNFATADIELQRKIVYAEM 162
DL P V++ L + ++ F ++ +E +R+ + +E+
Sbjct: 284 DSEDDLFPSVLDALNEIAFHPKFLSSRVEKERRAILSEL 322
>At3g13170.1 68416.m01647 DNA topoisomerase VIA (SPO11-1) identical
to AtSPO11-1 [Arabidopsis thaliana] GI:13383478;
contains Pfam profile PF04406: Type IIB DNA
topoisomerase; identical to cDNA putative topoisomerase
VIA (SPO11 gene 1) GI:7270974
Length = 362
Score = 31.1 bits (67), Expect = 1.6
Identities = 19/78 (24%), Positives = 41/78 (52%), Gaps = 2/78 (2%)
Query: 123 ADCL-SYDLPRVVEILTDCIYNNNFATADIELQRKIVYAEMIEQDKNSNTVLYDYLHSTA 181
ADCL S D P+ EI T + D+ L+ ++ ++++++++++ Y+H +A
Sbjct: 50 ADCLCSSDKPKGQEIFTLKKEPQTYRI-DMLLRVLLIVQQLLQENRHASKRDIYYMHPSA 108
Query: 182 FQGTPLAQTVMGPSCNLY 199
F+ + +G C L+
Sbjct: 109 FKAQSIVDRAIGDICILF 126
>At1g06900.1 68414.m00733 peptidase M16 family protein / insulinase
family protein contains Pfam domain, PF05193: Peptidase
M16 inactive domain; similar to insulin-degrading enzyme
(Insulysin, Insulinase, Insulin protease) [Mouse]
SWISS-PROT:Q9JHR7
Length = 1023
Score = 30.3 bits (65), Expect = 2.8
Identities = 15/71 (21%), Positives = 29/71 (40%), Gaps = 1/71 (1%)
Query: 53 ERESYNTCVGLFIDAGSRYEDNFENGVGHFFEHLAFKG-TKCRTKTLLEDQISSSGAIFK 111
+ ++ + + GS + G+ HF EH+ F G T+ + + +S G
Sbjct: 101 DHQTKKAAAAMCVSMGSFLDPPEAQGLAHFLEHMLFMGSTEFPDENEYDSYLSKHGGSSN 160
Query: 112 CFTTREMVAYY 122
+T E Y+
Sbjct: 161 AYTEMEHTCYH 171
>At3g11490.1 68416.m01401 rac GTPase activating protein, putative
similar to rac GTPase activating protein 1 GB:AAC62624
GI:3695059 [Lotus japonicus]; contains Pfam profile
PF00620: RhoGAP domain
Length = 435
Score = 29.1 bits (62), Expect = 6.6
Identities = 16/72 (22%), Positives = 31/72 (43%), Gaps = 3/72 (4%)
Query: 286 DQIVMEVAASIISGWDKSQPGGINHAV---RIAREASTDKFCDSYKAVNITYKDTSLWGV 342
D I + AS+I W + P G+ ++ ++ S D+ + + + T W +
Sbjct: 200 DNIDVHCLASLIKAWFRELPSGVLDSLSPEQVMESESEDECVELVRLLPSTEASLLDWAI 259
Query: 343 QFMGPSVELEDM 354
M VE+E +
Sbjct: 260 NLMADVVEMEQL 271
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.322 0.137 0.423
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,996,850
Number of Sequences: 28952
Number of extensions: 456316
Number of successful extensions: 954
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 942
Number of HSP's gapped (non-prelim): 9
length of query: 466
length of database: 12,070,560
effective HSP length: 84
effective length of query: 382
effective length of database: 9,638,592
effective search space: 3681942144
effective search space used: 3681942144
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 61 (28.7 bits)
- SilkBase 1999-2023 -