BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA002029-TA|BGIBMGA002029-PA|IPR004701|Phosphotransferase
system, fructose subfamily IIA component, IPR013919|Peroxisome
membrane protein, Pex16
(270 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At2g45690.1 68415.m05681 shrunken seed protein (SSE1) identical ... 62 4e-10
At1g80040.1 68414.m09369 expressed protein 32 0.35
At1g76890.2 68414.m08948 trihelix DNA-binding protein / GT-2 fac... 30 1.4
At1g76890.1 68414.m08947 trihelix DNA-binding protein / GT-2 fac... 30 1.4
At1g64790.1 68414.m07346 translational activator family protein ... 30 1.4
At4g36770.1 68417.m05217 UDP-glucoronosyl/UDP-glucosyl transfera... 30 1.9
At1g27910.1 68414.m03420 U-box domain-containing protein contain... 29 3.3
At5g01400.1 68418.m00053 expressed protein contains low similari... 28 5.8
At2g16430.2 68415.m01882 purple acid phosphatase (PAP10) identic... 28 5.8
At2g16430.1 68415.m01881 purple acid phosphatase (PAP10) identic... 28 5.8
At1g68670.1 68414.m07846 myb family transcription factor contain... 28 7.6
>At2g45690.1 68415.m05681 shrunken seed protein (SSE1) identical to
shrunken seed protein [Arabidopsis thaliana] GI:4837733;
annotation based on supporting cDNA
gi|4837732|gb|AF085354.1|AF085354
Length = 367
Score = 62.1 bits (144), Expect = 4e-10
Identities = 35/126 (27%), Positives = 69/126 (54%), Gaps = 9/126 (7%)
Query: 153 ESLHVLKPLLHLAAMRFFGNKAWKQWFVALSID------IASLKVYNRYMKELSYE--QR 204
E L++ +PL+++ +R +G ++W W ++LS+D +A+ K + K++ + ++
Sbjct: 243 EVLYITRPLIYVLFIRKYGVRSWIPWAISLSVDTLGMGLLANSKWWGEKSKQVHFSGPEK 302
Query: 205 LEISRRKLGLVLYLLRSPMYNKYSNTVIENVLNSASKKIPMMSLICGPIIQYLNHWQDIY 264
E+ RRKL LYL+R P + KY+ +E+ + IP++ + I++ L Q Y
Sbjct: 303 DELRRRKLIWALYLMRDPFFTKYTRQKLES-SQKKLELIPLIGFLTEKIVELLEGAQSRY 361
Query: 265 FYMWAS 270
Y+ S
Sbjct: 362 TYISGS 367
>At1g80040.1 68414.m09369 expressed protein
Length = 248
Score = 32.3 bits (70), Expect = 0.35
Identities = 31/120 (25%), Positives = 56/120 (46%), Gaps = 9/120 (7%)
Query: 121 GAPPIAFRDWTPVKIKDDRPVPGIEVKDLLYAESLHVLKPLLHLAAMRFFGNKAWKQWFV 180
G PP + DW + +++ G + + A L L+ +L A GNK +++ V
Sbjct: 107 GNPPTSGDDWVELLVREVLQSSGTDDAKVRAARVLEALEKMLSARAREEAGNK-FQEEKV 165
Query: 181 ALSIDIASLKVYNRYMKE---LSYEQR--LEISRRKLGLVLYLLRSPMY-NKYSNTVIEN 234
A+ + +L N +K + +E++ LE + +LGL+ L+ P Y K N + N
Sbjct: 166 AVQQQVETLVKDNTVLKRAVAIQHERQKALEDANHQLGLLKQLV--PQYQEKLRNLEVNN 223
>At1g76890.2 68414.m08948 trihelix DNA-binding protein / GT-2 factor
(GT2) identical to GT2 factor [Arabidopsis thaliana]
GI:416490, GI:2664202 (DNA binding factor GT-2 from
Arabidopsis)
Length = 575
Score = 30.3 bits (65), Expect = 1.4
Identities = 21/79 (26%), Positives = 39/79 (49%), Gaps = 5/79 (6%)
Query: 173 KAWKQWFVALSIDIASL--KVYNRYMKELSYEQRLEISRRKLGLVLYLLRSPMYNKYSNT 230
K WK F L+ ++ K+ R+++ L Y ++ ISR + V + R N+ T
Sbjct: 259 KYWKGLFTKLTKELMEKQEKMQKRFLETLEYREKERISREEAWRVQEIGR---INREHET 315
Query: 231 VIENVLNSASKKIPMMSLI 249
+I N+A+K ++S +
Sbjct: 316 LIHERSNAAAKDAAIISFL 334
>At1g76890.1 68414.m08947 trihelix DNA-binding protein / GT-2 factor
(GT2) identical to GT2 factor [Arabidopsis thaliana]
GI:416490, GI:2664202 (DNA binding factor GT-2 from
Arabidopsis)
Length = 498
Score = 30.3 bits (65), Expect = 1.4
Identities = 21/79 (26%), Positives = 39/79 (49%), Gaps = 5/79 (6%)
Query: 173 KAWKQWFVALSIDIASL--KVYNRYMKELSYEQRLEISRRKLGLVLYLLRSPMYNKYSNT 230
K WK F L+ ++ K+ R+++ L Y ++ ISR + V + R N+ T
Sbjct: 182 KYWKGLFTKLTKELMEKQEKMQKRFLETLEYREKERISREEAWRVQEIGR---INREHET 238
Query: 231 VIENVLNSASKKIPMMSLI 249
+I N+A+K ++S +
Sbjct: 239 LIHERSNAAAKDAAIISFL 257
>At1g64790.1 68414.m07346 translational activator family protein
similar to HsGCN1 [Homo sapiens] GI:2282576
Length = 2440
Score = 30.3 bits (65), Expect = 1.4
Identities = 24/79 (30%), Positives = 41/79 (51%), Gaps = 7/79 (8%)
Query: 188 SLKVYNRYMKELSYEQ-RLEISRRKLGLVL-YLLRSPMYNKYSNTV-----IENVLNSAS 240
S +Y+ YM E+ + + S LGL+L + SP + S + +++VLNS
Sbjct: 104 SQAIYSLYMDEVKGSRIPYKDSPELLGLLLEFSCSSPALFEQSKAIFVDIYVKDVLNSRE 163
Query: 241 KKIPMMSLICGPIIQYLNH 259
K+ P +S P++Q L+H
Sbjct: 164 KQKPNLSNCFKPLLQRLSH 182
>At4g36770.1 68417.m05217 UDP-glucoronosyl/UDP-glucosyl transferase
family protein contains Pfam profile: PF00201
UDP-glucoronosyl and UDP-glucosyl transferase
Length = 457
Score = 29.9 bits (64), Expect = 1.9
Identities = 23/61 (37%), Positives = 30/61 (49%), Gaps = 4/61 (6%)
Query: 140 PVPGIEVKDLLYAESLHVLKPLLHLAAMRFFGNKAWKQWFVALSIDIASLKVYNRYMKEL 199
P P + V DLL E+L V K L MR WF+A ++ +ASL Y K+L
Sbjct: 104 PRPRVFVVDLLGTEALEVAK---ELGIMRKHVLVTTSAWFLAFTVYMASLDKQELY-KQL 159
Query: 200 S 200
S
Sbjct: 160 S 160
>At1g27910.1 68414.m03420 U-box domain-containing protein contains
Pfam profile PF04564: U-box domain
Length = 768
Score = 29.1 bits (62), Expect = 3.3
Identities = 20/62 (32%), Positives = 29/62 (46%), Gaps = 3/62 (4%)
Query: 194 RYMKELSYEQRLEISRRKLGLVLYLLRSPMYNKYSNTVIENVLNSASKKIPMMSLICGPI 253
R +K+L R+E +RK +V YLL + KYS + + + SL C P
Sbjct: 183 RCLKKLIERARMEDDKRKESIVAYLLH--LMRKYSKLFRSEIWDDNDSQ-GSSSLPCSPT 239
Query: 254 IQ 255
IQ
Sbjct: 240 IQ 241
>At5g01400.1 68418.m00053 expressed protein contains low similarity
to symplekin SP:Q92797 from [Homo sapiens]
Length = 1467
Score = 28.3 bits (60), Expect = 5.8
Identities = 16/48 (33%), Positives = 24/48 (50%)
Query: 210 RKLGLVLYLLRSPMYNKYSNTVIENVLNSASKKIPMMSLICGPIIQYL 257
+KLGL+L LR P +++ I ++NS S CG I+ L
Sbjct: 206 QKLGLLLDQLRHPAAKSLNSSTIIVLINSLSSVAKKRPAYCGRILPVL 253
>At2g16430.2 68415.m01882 purple acid phosphatase (PAP10) identical
to purple acid phosphatase (PAP10) GI:20257482 from
[Arabidopsis thaliana]
Length = 468
Score = 28.3 bits (60), Expect = 5.8
Identities = 15/55 (27%), Positives = 28/55 (50%), Gaps = 2/55 (3%)
Query: 181 ALSIDIASLKVYNRYMKELSY--EQRLEISRRKLGLVLYLLRSPMYNKYSNTVIE 233
A I +AS Y +Y + + E+ +++R + ++ L+ SP YN Y +E
Sbjct: 277 AYIIVLASYSAYGKYTPQYQWLEEEFPKVNRTETPWLIVLMHSPWYNSYDYHYME 331
>At2g16430.1 68415.m01881 purple acid phosphatase (PAP10) identical
to purple acid phosphatase (PAP10) GI:20257482 from
[Arabidopsis thaliana]
Length = 348
Score = 28.3 bits (60), Expect = 5.8
Identities = 15/55 (27%), Positives = 28/55 (50%), Gaps = 2/55 (3%)
Query: 181 ALSIDIASLKVYNRYMKELSY--EQRLEISRRKLGLVLYLLRSPMYNKYSNTVIE 233
A I +AS Y +Y + + E+ +++R + ++ L+ SP YN Y +E
Sbjct: 157 AYIIVLASYSAYGKYTPQYQWLEEEFPKVNRTETPWLIVLMHSPWYNSYDYHYME 211
>At1g68670.1 68414.m07846 myb family transcription factor contains
Pfam domain, PF00249: Myb-like DNA-binding domain
Length = 354
Score = 27.9 bits (59), Expect = 7.6
Identities = 20/61 (32%), Positives = 28/61 (45%), Gaps = 3/61 (4%)
Query: 131 TPVKIKDDRPVPGI---EVKDLLYAESLHVLKPLLHLAAMRFFGNKAWKQWFVALSIDIA 187
TP +I+D V G+ EVK L LH +P A + GN+ Q+ V I +
Sbjct: 242 TPKQIRDHMKVDGLTNDEVKSHLQKYRLHTRRPAATSVAAQSTGNQQQPQFVVVGGIWVP 301
Query: 188 S 188
S
Sbjct: 302 S 302
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.323 0.136 0.420
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,436,593
Number of Sequences: 28952
Number of extensions: 256214
Number of successful extensions: 516
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 510
Number of HSP's gapped (non-prelim): 14
length of query: 270
length of database: 12,070,560
effective HSP length: 80
effective length of query: 190
effective length of database: 9,754,400
effective search space: 1853336000
effective search space used: 1853336000
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (22.0 bits)
S2: 59 (27.9 bits)
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