Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA002020-TA|BGIBMGA002020-PA|IPR006076|FAD dependent
oxidoreductase, IPR006222|Glycine cleavage T protein (aminomethyl
transferase), IPR013977|Glycine cleavage T-protein, C-terminal barrel
(975 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g11860.2 68414.m01366 aminomethyltransferase, putative simila... 71 4e-12
At1g11860.1 68414.m01365 aminomethyltransferase, putative simila... 71 4e-12
At5g67290.1 68418.m08484 FAD-dependent oxidoreductase family pro... 36 0.13
At3g04440.1 68416.m00470 expressed protein contains Pfam domain,... 34 0.53
At3g03700.1 68416.m00373 expressed protein contains Pfam domain,... 34 0.53
At5g13610.1 68418.m01576 expressed protein 33 0.70
At5g48440.2 68418.m05990 FAD-dependent oxidoreductase family pro... 33 0.92
At5g48440.1 68418.m05989 FAD-dependent oxidoreductase family pro... 33 0.92
At2g24580.1 68415.m02935 sarcosine oxidase family protein simila... 33 1.2
At4g26680.1 68417.m03844 pentatricopeptide (PPR) repeat-containi... 32 1.6
At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate dehy... 31 3.7
At5g24155.1 68418.m02841 squalene monooxygenase, putative / squa... 31 4.9
At1g33990.1 68414.m04214 hydrolase, alpha/beta fold family prote... 30 6.5
At3g23530.1 68416.m02961 cyclopropane fatty acid synthase, putat... 30 8.6
At3g23510.1 68416.m02960 cyclopropane fatty acid synthase, putat... 30 8.6
>At1g11860.2 68414.m01366 aminomethyltransferase, putative similar
to aminomethyltransferase, mitochondrial precursor
SP:O49849 from [Flaveria anomala]
Length = 408
Score = 70.9 bits (166), Expect = 4e-12
Identities = 69/329 (20%), Positives = 134/329 (40%), Gaps = 18/329 (5%)
Query: 593 DAVQREYWACRERVGLSDYSSFTKIDIQSQGREVVELLQYLCSNDV-DVPVGSIIHTGMQ 651
D++ CRE L D + + ++ G++ V L+ L DV + G+ T
Sbjct: 66 DSIMDSTVNCRENGSLFDVAHMCGLSLK--GKDCVPFLETLVVADVAGLAPGTGSLTVFT 123
Query: 652 NERGGYENDCSLARISENHYMMIAPTIQQTRCKVWLKRHLP---SNGSVTLSDVTSMYTA 708
NE+GG +D + ++++ H ++ + + ++ H+ S G + +
Sbjct: 124 NEKGGAIDDSVITKVTDEHIYLVVNAGCRDKDLAHIEEHMKAFKSKGGDVSWHIHDERSL 183
Query: 709 ICVMGPFTRXXXXXXXXXXXXXXNFPFFTFKEIDVGLANGIRAMNLTHTGELGYVLYIPN 768
+ + GP F F +I+ R +TGE G+ + +P+
Sbjct: 184 LALQGPLAAPVLQHLTKEDLSKLYFGNFQILDINGSTCFLTRT---GYTGEDGFEISVPD 240
Query: 769 EFALHVYNRLMTVGEKYGISHVGYYASRALRVEKFFAFWGQDLDTMTTPLECGRTWRVKF 828
E A+ + ++ E + G A +LR+E +G D++ +P+E G TW +
Sbjct: 241 EHAVDLAKAILEKSEGK-VRLTGLGARDSLRLEAGLCLYGNDMEQHISPVEAGLTWAIGK 299
Query: 829 DKDIK--FIGRDALLKQREDGIRRQYVQXXXXXXXXXXXXWSWGGEPIYRDGNYCGQTTT 886
+ + F+G D +L+Q +DG + V E GN G+ T+
Sbjct: 300 RRRAEGGFLGADVILQQLKDGPTIRRVGFFSSGPPARSH-----SEVHDESGNKIGEITS 354
Query: 887 TSYGFTFKKQVCLGFVEK-RDKDGVTQKV 914
+ KK + +G+V+ + K G K+
Sbjct: 355 GGFSPNLKKNIAMGYVKSGQHKTGTKVKI 383
>At1g11860.1 68414.m01365 aminomethyltransferase, putative similar
to aminomethyltransferase, mitochondrial precursor
SP:O49849 from [Flaveria anomala]
Length = 408
Score = 70.9 bits (166), Expect = 4e-12
Identities = 69/329 (20%), Positives = 134/329 (40%), Gaps = 18/329 (5%)
Query: 593 DAVQREYWACRERVGLSDYSSFTKIDIQSQGREVVELLQYLCSNDV-DVPVGSIIHTGMQ 651
D++ CRE L D + + ++ G++ V L+ L DV + G+ T
Sbjct: 66 DSIMDSTVNCRENGSLFDVAHMCGLSLK--GKDCVPFLETLVVADVAGLAPGTGSLTVFT 123
Query: 652 NERGGYENDCSLARISENHYMMIAPTIQQTRCKVWLKRHLP---SNGSVTLSDVTSMYTA 708
NE+GG +D + ++++ H ++ + + ++ H+ S G + +
Sbjct: 124 NEKGGAIDDSVITKVTDEHIYLVVNAGCRDKDLAHIEEHMKAFKSKGGDVSWHIHDERSL 183
Query: 709 ICVMGPFTRXXXXXXXXXXXXXXNFPFFTFKEIDVGLANGIRAMNLTHTGELGYVLYIPN 768
+ + GP F F +I+ R +TGE G+ + +P+
Sbjct: 184 LALQGPLAAPVLQHLTKEDLSKLYFGNFQILDINGSTCFLTRT---GYTGEDGFEISVPD 240
Query: 769 EFALHVYNRLMTVGEKYGISHVGYYASRALRVEKFFAFWGQDLDTMTTPLECGRTWRVKF 828
E A+ + ++ E + G A +LR+E +G D++ +P+E G TW +
Sbjct: 241 EHAVDLAKAILEKSEGK-VRLTGLGARDSLRLEAGLCLYGNDMEQHISPVEAGLTWAIGK 299
Query: 829 DKDIK--FIGRDALLKQREDGIRRQYVQXXXXXXXXXXXXWSWGGEPIYRDGNYCGQTTT 886
+ + F+G D +L+Q +DG + V E GN G+ T+
Sbjct: 300 RRRAEGGFLGADVILQQLKDGPTIRRVGFFSSGPPARSH-----SEVHDESGNKIGEITS 354
Query: 887 TSYGFTFKKQVCLGFVEK-RDKDGVTQKV 914
+ KK + +G+V+ + K G K+
Sbjct: 355 GGFSPNLKKNIAMGYVKSGQHKTGTKVKI 383
>At5g67290.1 68418.m08484 FAD-dependent oxidoreductase family
protein contains Pfam profile PF01266: FAD dependent
oxidoreductase
Length = 406
Score = 35.9 bits (79), Expect = 0.13
Identities = 17/47 (36%), Positives = 25/47 (53%), Gaps = 2/47 (4%)
Query: 51 KVVICGGGVMGAAVAYHLANRGWGDRTVVVEKEKVGAGSRWHSSGLV 97
+V +CGGGV+G AY LA +G +VEK V + + G +
Sbjct: 41 RVFVCGGGVIGVCTAYFLAKKGIA--VTLVEKSAVACAASGKAGGFL 85
>At3g04440.1 68416.m00470 expressed protein contains Pfam domain,
PF04515: Protein of unknown function, DUF580
Length = 482
Score = 33.9 bits (74), Expect = 0.53
Identities = 19/57 (33%), Positives = 31/57 (54%), Gaps = 1/57 (1%)
Query: 737 TFKEIDVGLANGIRAMNLTHTGELGYVLYIPNEFALHVYNRLMTVGEKYGISHVGYY 793
T I V + IR++NL +G V+Y + + N+L+T+G ++G HVG Y
Sbjct: 287 TLVPIIVFIRGSIRSVNLM-SGSSDEVMYSGADCFSTIANKLITLGNRWGFVHVGTY 342
>At3g03700.1 68416.m00373 expressed protein contains Pfam domain,
PF04515: Protein of unknown function, DUF580
Length = 482
Score = 33.9 bits (74), Expect = 0.53
Identities = 19/57 (33%), Positives = 31/57 (54%), Gaps = 1/57 (1%)
Query: 737 TFKEIDVGLANGIRAMNLTHTGELGYVLYIPNEFALHVYNRLMTVGEKYGISHVGYY 793
T I V + IR++NL +G V+Y + + N+L+T+G ++G HVG Y
Sbjct: 287 TLVPIIVFIRGSIRSVNLM-SGSSDEVMYSGADCFSTIANKLITLGNRWGFVHVGTY 342
>At5g13610.1 68418.m01576 expressed protein
Length = 402
Score = 33.5 bits (73), Expect = 0.70
Identities = 27/98 (27%), Positives = 44/98 (44%), Gaps = 6/98 (6%)
Query: 390 YSTSDSIVSVHLLRRLRMGFRPPRGVSTNEI-----NHKNPEEISDTLVSNSRSVKVVIF 444
Y S S+ L+ + + F PP TN + NH +P + + +S S S+ +V+F
Sbjct: 126 YFFSTSVDLRSLIEQNKQNFIPPTSRMTNYVVLKFGNHSDPTDTTRGRISGSESIYMVVF 185
Query: 445 PISLSERRFQMLNYHVAAGMKTVGISAAGGVAEATVDE 482
S F + + V +K V A+G + E DE
Sbjct: 186 HYG-SIVLFNVREHEVDEYLKVVERHASGLLPEMRKDE 222
>At5g48440.2 68418.m05990 FAD-dependent oxidoreductase family
protein low similarity to hydrogen cyanide synthase HcnC
from Pseudomonas fluorescens [GI:3220203]; contains Pfam
profile PF01266 FAD dependent oxidoreductase
Length = 333
Score = 33.1 bits (72), Expect = 0.92
Identities = 17/69 (24%), Positives = 31/69 (44%)
Query: 128 GWKQCGSLLLARTRDRMTVYRRMKSQSVSWSIDCDLVTPKKCHELFPMLNVEDVLGGLWI 187
GWK+ GSLL+ RT + ++ + + + ++ + P + V D G ++
Sbjct: 40 GWKKTGSLLIGRTTEECVALKQKVHELSEAGLRTEYLSSAELLLKEPAILVNDNTGAAFL 99
Query: 188 PGDGVGDPH 196
P D D H
Sbjct: 100 PDDSQLDAH 108
>At5g48440.1 68418.m05989 FAD-dependent oxidoreductase family
protein low similarity to hydrogen cyanide synthase HcnC
from Pseudomonas fluorescens [GI:3220203]; contains Pfam
profile PF01266 FAD dependent oxidoreductase
Length = 367
Score = 33.1 bits (72), Expect = 0.92
Identities = 17/69 (24%), Positives = 31/69 (44%)
Query: 128 GWKQCGSLLLARTRDRMTVYRRMKSQSVSWSIDCDLVTPKKCHELFPMLNVEDVLGGLWI 187
GWK+ GSLL+ RT + ++ + + + ++ + P + V D G ++
Sbjct: 40 GWKKTGSLLIGRTTEECVALKQKVHELSEAGLRTEYLSSAELLLKEPAILVNDNTGAAFL 99
Query: 188 PGDGVGDPH 196
P D D H
Sbjct: 100 PDDSQLDAH 108
>At2g24580.1 68415.m02935 sarcosine oxidase family protein similar
to peroxisomal sarcosine oxidase from Homo sapiens
[SP|Q9P0Z9], Oryctolagus cuniculus [SP|P79371], Mus
musculus [SP|Q9D826]
Length = 416
Score = 32.7 bits (71), Expect = 1.2
Identities = 14/31 (45%), Positives = 23/31 (74%), Gaps = 2/31 (6%)
Query: 52 VVICGGGVMGAAVAYHLANRGWGDRTVVVEK 82
V++ G GVMG++ AY LA R G +T+++E+
Sbjct: 11 VIVVGAGVMGSSAAYQLAKR--GQKTLLLEQ 39
>At4g26680.1 68417.m03844 pentatricopeptide (PPR) repeat-containing
protein contains Pfam profile PF01535: PPR repeat
Length = 521
Score = 32.3 bits (70), Expect = 1.6
Identities = 13/36 (36%), Positives = 24/36 (66%)
Query: 330 CLPEDWDHFHVLLQELLQRVPGLNQAVLHKLCNGLE 365
C ED+D +L+E+++R L+ +H++CNGL+
Sbjct: 459 CRNEDFDGASQVLREMVRRSIPLDSRTVHQVCNGLK 494
>At2g40690.1 68415.m05020 NAD-dependent glycerol-3-phosphate
dehydrogenase family protein low similarity to SP|Q26756
Glycerol-3-phosphate dehydrogenase [NAD+], glycosomal
(EC 1.1.1.8) {Trypanosoma brucei rhodesiense}; contains
Pfam profile PF01210: NAD-dependent glycerol-3-phosphate
dehydrogenase
Length = 420
Score = 31.1 bits (67), Expect = 3.7
Identities = 15/30 (50%), Positives = 18/30 (60%)
Query: 45 VLPSKAKVVICGGGVMGAAVAYHLANRGWG 74
VL KVV+ GGG G A+A H+A R G
Sbjct: 83 VLERTRKVVVLGGGSFGTAMAAHVARRKEG 112
>At5g24155.1 68418.m02841 squalene monooxygenase, putative /
squalene epoxidase, putative similar to SP|O65404 (SE
1,1), SP|O65402 (SE 1,2)
Length = 121
Score = 30.7 bits (66), Expect = 4.9
Identities = 16/36 (44%), Positives = 23/36 (63%), Gaps = 2/36 (5%)
Query: 48 SKAKVVICGGGVMGAAVAYHLANRGWGDRTVVVEKE 83
S A V+I G GV G+A+AY LA G R + +E++
Sbjct: 46 SAADVIIVGAGVGGSALAYSLAKD--GRRVLAIERD 79
>At1g33990.1 68414.m04214 hydrolase, alpha/beta fold family protein
similar to polyneuridine aldehyde esterase GI:6651393
from [Rauvolfia serpentina], SP|Q40708 PIR7A protein
{Oryza sativa}, ethylene-induced esterase [Citrus
sinensis] GI:14279437; contains Pfam profile PF00561:
hydrolase, alpha/beta fold family
Length = 348
Score = 30.3 bits (65), Expect = 6.5
Identities = 13/35 (37%), Positives = 21/35 (60%)
Query: 34 DYEEKLEDCLSVLPSKAKVVICGGGVMGAAVAYHL 68
+Y + L D L LP + KV++ G GA+++Y L
Sbjct: 148 EYSKPLIDLLENLPEEEKVILVGHSTGGASISYAL 182
>At3g23530.1 68416.m02961 cyclopropane fatty acid synthase,
putative / CPA-FA synthase, putative similar to
cyclopropane synthase [Sterculia foetida] GI:21069167;
contains Pfam profiles PF02353:
Cyclopropane-fatty-acyl-phospholipid synthase, PF01593:
amine oxidase, flavin-containing
Length = 867
Score = 29.9 bits (64), Expect = 8.6
Identities = 13/40 (32%), Positives = 23/40 (57%)
Query: 51 KVVICGGGVMGAAVAYHLANRGWGDRTVVVEKEKVGAGSR 90
KV + G G+ G AY LAN+G + + ++E +G ++
Sbjct: 2 KVAVIGSGISGLGSAYVLANQGVKEVVLYEKEESLGGHAK 41
>At3g23510.1 68416.m02960 cyclopropane fatty acid synthase,
putative / CPA-FA synthase, putative similar to
cyclopropane synthase [Sterculia foetida] GI:21069167;
contains Pfam profile PF02353:
Cyclopropane-fatty-acyl-phospholipid synthase
Length = 867
Score = 29.9 bits (64), Expect = 8.6
Identities = 13/40 (32%), Positives = 23/40 (57%)
Query: 51 KVVICGGGVMGAAVAYHLANRGWGDRTVVVEKEKVGAGSR 90
KV + G G+ G AY LAN+G + + ++E +G ++
Sbjct: 2 KVAVIGSGISGLGSAYVLANQGVKEVVLYEKEESLGGHAK 41
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.321 0.139 0.435
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 24,205,852
Number of Sequences: 28952
Number of extensions: 1083261
Number of successful extensions: 2098
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 2081
Number of HSP's gapped (non-prelim): 18
length of query: 975
length of database: 12,070,560
effective HSP length: 88
effective length of query: 887
effective length of database: 9,522,784
effective search space: 8446709408
effective search space used: 8446709408
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 64 (29.9 bits)
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