Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001977-TA|BGIBMGA001977-PA|IPR004481|K+-dependent
Na+/Ca+ exchanger related-protein, IPR004837|Sodium/calcium exchanger
membrane region
(254 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_15681| Best HMM Match : Na_Ca_ex (HMM E-Value=1.9e-36) 219 2e-57
SB_43713| Best HMM Match : Na_Ca_ex (HMM E-Value=7.9e-32) 178 3e-45
SB_42413| Best HMM Match : No HMM Matches (HMM E-Value=.) 165 4e-41
SB_45810| Best HMM Match : Na_Ca_ex (HMM E-Value=0) 152 2e-37
SB_42414| Best HMM Match : No HMM Matches (HMM E-Value=.) 52 6e-07
SB_15333| Best HMM Match : No HMM Matches (HMM E-Value=.) 50 1e-06
SB_44420| Best HMM Match : Calx-beta (HMM E-Value=2e-22) 49 3e-06
SB_8443| Best HMM Match : Calx-beta (HMM E-Value=0) 49 4e-06
SB_28247| Best HMM Match : Na_Ca_ex (HMM E-Value=2e-27) 35 0.056
SB_13374| Best HMM Match : Cornifin (HMM E-Value=0.34) 31 0.69
SB_32754| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 3.7
SB_42894| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.9
SB_47960| Best HMM Match : Extensin_2 (HMM E-Value=0.54) 29 4.9
SB_16336| Best HMM Match : 7tm_1 (HMM E-Value=5.9e-18) 28 6.5
SB_59527| Best HMM Match : DUF382 (HMM E-Value=4.1e-26) 28 6.5
SB_34821| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 8.5
SB_36054| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 8.5
>SB_15681| Best HMM Match : Na_Ca_ex (HMM E-Value=1.9e-36)
Length = 183
Score = 219 bits (535), Expect = 2e-57
Identities = 110/176 (62%), Positives = 134/176 (76%), Gaps = 4/176 (2%)
Query: 63 MGYMFVALAIVCDEFFVPALDVIIERLEIRDDVAGATFMAAGGSAPELFTSVIGVFVS-F 121
M YMF+ALAI CDEFFVP+L VIIE+LE+++DVAGATFMAAGGSAPELFTS IG F+
Sbjct: 1 MCYMFLALAIACDEFFVPSLTVIIEKLELKEDVAGATFMAAGGSAPELFTSFIGTFIDPK 60
Query: 122 DDVGIGTIVGSAVFNILFVIGACAMFSRTTLSLTWWPLFRDCTFYSISLLVLIYCF-RDN 180
+VGIGTIVGSAVFN+LFVIG CAMFS+ L LTWWPLFRDC FYS++L++LI F +
Sbjct: 61 SNVGIGTIVGSAVFNVLFVIGMCAMFSKGVLELTWWPLFRDCIFYSLALIILIIFFLSGD 120
Query: 181 RIYWQEALVLFSLYGAYVMFMRWNQPVERAFKKLIYKNKVTR--VRSTDQLMPTVS 234
I W E+LVL Y AYV+FM++N VER K L NKV+ + S + + P V+
Sbjct: 121 VIVWWESLVLLLCYFAYVIFMKYNHNVERRIKGLFGSNKVSSMDMSSAEAINPGVN 176
>SB_43713| Best HMM Match : Na_Ca_ex (HMM E-Value=7.9e-32)
Length = 228
Score = 178 bits (434), Expect = 3e-45
Identities = 81/163 (49%), Positives = 111/163 (68%)
Query: 49 EQRRSGAVLLHVLGMGYMFVALAIVCDEFFVPALDVIIERLEIRDDVAGATFMAAGGSAP 108
++++ G ++ HV Y F ALA VCDE+FV +L+ I + L ++ DVAGATFMAAG SAP
Sbjct: 5 QRQKQGGIVFHVFVAIYTFAALATVCDEYFVSSLERICDGLNLKSDVAGATFMAAGSSAP 64
Query: 109 ELFTSVIGVFVSFDDVGIGTIVGSAVFNILFVIGACAMFSRTTLSLTWWPLFRDCTFYSI 168
E FTSVIGVF++ D+GIGTIVGSAVFNILF++ C MF+ + L+L+WWPL RD Y +
Sbjct: 65 EFFTSVIGVFITKGDIGIGTIVGSAVFNILFIVAICGMFAGSVLTLSWWPLTRDSFCYML 124
Query: 169 SLLVLIYCFRDNRIYWQEALVLFSLYGAYVMFMRWNQPVERAF 211
S+ L+ D ++W E + L +Y YV M +N +E F
Sbjct: 125 SVAALVIITYDKEVHWYEGMALVLMYLLYVTIMYFNSTLEGFF 167
>SB_42413| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 639
Score = 165 bits (400), Expect = 4e-41
Identities = 76/168 (45%), Positives = 112/168 (66%)
Query: 45 VFSEEQRRSGAVLLHVLGMGYMFVALAIVCDEFFVPALDVIIERLEIRDDVAGATFMAAG 104
+F+ ++RR GAV++H Y FVALAI+CD +F +L+ + +RL I DVAGATFMAAG
Sbjct: 39 IFTLQERRHGAVIIHTFICLYCFVALAILCDNYFCASLEKLCKRLRIPTDVAGATFMAAG 98
Query: 105 GSAPELFTSVIGVFVSFDDVGIGTIVGSAVFNILFVIGACAMFSRTTLSLTWWPLFRDCT 164
S P LF ++ VF+ D+G+GTI+GS++FNILF+ C +FS +SL WP+ RD
Sbjct: 99 SSMPTLFIAIASVFMGEGDIGLGTIIGSSMFNILFITAICGLFSGMVISLHTWPIVRDSC 158
Query: 165 FYSISLLVLIYCFRDNRIYWQEALVLFSLYGAYVMFMRWNQPVERAFK 212
Y ++L+ L+ DN I++ EAL+ LY Y++ M +N +E AF+
Sbjct: 159 VYVVNLVGLLIVIHDNVIHFYEALIFPVLYTGYILIMVFNTRLENAFE 206
Score = 39.1 bits (87), Expect = 0.003
Identities = 17/57 (29%), Positives = 32/57 (56%)
Query: 82 LDVIIERLEIRDDVAGATFMAAGGSAPELFTSVIGVFVSFDDVGIGTIVGSAVFNIL 138
+ +I E I + + G T +AAG S P++ +S+I D+ + +GS +F++L
Sbjct: 471 VSIIGETFSIPEYIMGLTLLAAGSSVPDVMSSLIVAKHGMGDMALANCIGSNIFDVL 527
>SB_45810| Best HMM Match : Na_Ca_ex (HMM E-Value=0)
Length = 582
Score = 152 bits (369), Expect = 2e-37
Identities = 76/137 (55%), Positives = 96/137 (70%), Gaps = 1/137 (0%)
Query: 68 VALAIVCDEFFVPALDVIIERLEIRDDVAGATFMAAGGSAPELFTSVIGVFVSFDDVGIG 127
+ALAIVCD+FFVP+L+ I E+L++ +DVAGATFMAAG SAPELFTS+ GV V DVG+G
Sbjct: 29 LALAIVCDDFFVPSLEAISEKLDLSEDVAGATFMAAGSSAPELFTSIAGVTVD-SDVGVG 87
Query: 128 TIVGSAVFNILFVIGACAMFSRTTLSLTWWPLFRDCTFYSISLLVLIYCFRDNRIYWQEA 187
TIVGSA+FN+L +I A FS L L W PL RD YS+S+ I D + EA
Sbjct: 88 TIVGSAIFNLLVIIALTAAFSGQVLQLDWRPLLRDSVAYSLSIAFFIIFSWDGKFEMYEA 147
Query: 188 LVLFSLYGAYVMFMRWN 204
VL LY AY++ M++N
Sbjct: 148 AVLLILYIAYIVVMKFN 164
Score = 32.3 bits (70), Expect = 0.40
Identities = 15/46 (32%), Positives = 24/46 (52%)
Query: 97 GATFMAAGGSAPELFTSVIGVFVSFDDVGIGTIVGSAVFNILFVIG 142
G +A G S P+ +S+I F D+ + +GS VF+I +G
Sbjct: 446 GLVIIAIGTSVPDALSSIIVARDGFGDMAVSNAIGSNVFDINLGLG 491
>SB_42414| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 360
Score = 51.6 bits (118), Expect = 6e-07
Identities = 25/67 (37%), Positives = 38/67 (56%), Gaps = 4/67 (5%)
Query: 157 WPLFRDCTFYSISLLVLIYCFRDNRIYWQEALVLFSLYGAYVMFMRWNQPVE---RAFKK 213
W + RD T Y S++VL+ DN++YW E L+ +Y Y++ M +N VE + KK
Sbjct: 57 WKM-RDSTVYVASVIVLMVSIYDNKVYWYEGLIFVIVYAIYIVIMYFNDQVEGLCKTVKK 115
Query: 214 LIYKNKV 220
+ K KV
Sbjct: 116 KLKKKKV 122
Score = 37.5 bits (83), Expect = 0.011
Identities = 21/66 (31%), Positives = 33/66 (50%)
Query: 73 VCDEFFVPALDVIIERLEIRDDVAGATFMAAGGSAPELFTSVIGVFVSFDDVGIGTIVGS 132
V F V + +I I + V G TF+AAG S P+ S++ D+ + +GS
Sbjct: 210 VLSYFLVWMVAIIGYTYTIPECVMGMTFLAAGSSLPDAIASLVVAKQGSGDMAVSNCIGS 269
Query: 133 AVFNIL 138
VF++L
Sbjct: 270 NVFDML 275
>SB_15333| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 752
Score = 50.4 bits (115), Expect = 1e-06
Identities = 40/116 (34%), Positives = 58/116 (50%), Gaps = 28/116 (24%)
Query: 58 LHVLGMGYMFVALAIVCDEFFVPALDVII--------------ERLEIR-----DDVAGA 98
L++LGM Y+F+ +AIV D F+ ++VI E +EI + VA
Sbjct: 40 LYILGMLYLFLGIAIVAD-IFMSCIEVITSKKRKVTRYDHEKGESVEIEVFVWNETVANL 98
Query: 99 TFMAAGGSAPELFTSVI--------GVFVSFDDVGIGTIVGSAVFNILFVIGACAM 146
T MA G SAPE+ +V+ G + D +G TIVGSA FN+L + C +
Sbjct: 99 TLMALGSSAPEILLAVVETGQELALGQTTATDGLGTFTIVGSASFNLLLITAVCVV 154
>SB_44420| Best HMM Match : Calx-beta (HMM E-Value=2e-22)
Length = 461
Score = 49.2 bits (112), Expect = 3e-06
Identities = 31/69 (44%), Positives = 42/69 (60%), Gaps = 8/69 (11%)
Query: 85 IIERLEIR---DDVAGATFMAAGGSAPELFTSVIGVFV--SFDDVGIG--TIVGSAVFNI 137
IIE +E+R + VA T MA G SAPE+ S+I + + F+ +G TIVGSA FN+
Sbjct: 86 IIE-VEVRVWNETVANLTLMALGSSAPEILLSIIEITIMNGFEAGALGPSTIVGSAAFNL 144
Query: 138 LFVIGACAM 146
L + G C M
Sbjct: 145 LCITGVCVM 153
>SB_8443| Best HMM Match : Calx-beta (HMM E-Value=0)
Length = 694
Score = 48.8 bits (111), Expect = 4e-06
Identities = 25/55 (45%), Positives = 32/55 (58%), Gaps = 3/55 (5%)
Query: 93 DDVAGATFMAAGGSAPELFTSVIGVF---VSFDDVGIGTIVGSAVFNILFVIGAC 144
+ V+ T MA G SAPE+ SVI + D+G TIVGSA FN+ +IG C
Sbjct: 64 ETVSNLTLMALGSSAPEILLSVIEIIGNNFKAGDLGPSTIVGSAAFNLFVIIGVC 118
>SB_28247| Best HMM Match : Na_Ca_ex (HMM E-Value=2e-27)
Length = 236
Score = 35.1 bits (77), Expect = 0.056
Identities = 18/52 (34%), Positives = 27/52 (51%)
Query: 91 IRDDVAGATFMAAGGSAPELFTSVIGVFVSFDDVGIGTIVGSAVFNILFVIG 142
I D V G + +A G S P+ +S+ D+ + VGS VF+IL +G
Sbjct: 98 IPDSVMGLSLVAFGSSVPDCLSSLFVARKGDGDMAVSHTVGSNVFDILLCLG 149
>SB_13374| Best HMM Match : Cornifin (HMM E-Value=0.34)
Length = 1197
Score = 31.5 bits (68), Expect = 0.69
Identities = 12/28 (42%), Positives = 19/28 (67%)
Query: 212 KKLIYKNKVTRVRSTDQLMPTVSSPAHP 239
K ++ KNK+ R+RS+ +L+P S HP
Sbjct: 46 KPIVNKNKIERLRSSKRLLPVDSHECHP 73
>SB_32754| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 5659
Score = 29.1 bits (62), Expect = 3.7
Identities = 11/32 (34%), Positives = 18/32 (56%)
Query: 18 PNATQLPNATQLPNATEDAKTPLFPPDVFSEE 49
P +T++PN+T +P T K+ PP + E
Sbjct: 2375 PESTEIPNSTVVPGTTISPKSTEVPPTTMTAE 2406
>SB_42894| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 533
Score = 28.7 bits (61), Expect = 4.9
Identities = 16/56 (28%), Positives = 28/56 (50%), Gaps = 2/56 (3%)
Query: 111 FTSVIGVFVSFDDVGIGTIVGSAVFNILFVIGACAMFSRTTLSLTWWPLFRDCTFY 166
F S VF G+ + +G+ + ++ G C SRT L+++W P+F +Y
Sbjct: 235 FYSERTVFAFSGGTGL-SFIGTTYYTVVTTYG-CLSPSRTMLTVSWLPVFIPLCYY 288
>SB_47960| Best HMM Match : Extensin_2 (HMM E-Value=0.54)
Length = 710
Score = 28.7 bits (61), Expect = 4.9
Identities = 16/49 (32%), Positives = 26/49 (53%), Gaps = 3/49 (6%)
Query: 171 LVLIYCFRDNRIYWQEALV--LFSLYGAYVMFMRWNQPVERAFKKLIYK 217
L+ +Y F YW+ V +FSL A++++ QPV +L+YK
Sbjct: 102 LIPVY-FNVKLHYWRMMAVWTVFSLATAFIVYKATRQPVSTTTPRLVYK 149
>SB_16336| Best HMM Match : 7tm_1 (HMM E-Value=5.9e-18)
Length = 344
Score = 28.3 bits (60), Expect = 6.5
Identities = 12/32 (37%), Positives = 18/32 (56%)
Query: 53 SGAVLLHVLGMGYMFVALAIVCDEFFVPALDV 84
+G VL H++G F A A+ CD + +L V
Sbjct: 105 AGNVLAHLIGSPSFFTATALSCDRYLAISLGV 136
>SB_59527| Best HMM Match : DUF382 (HMM E-Value=4.1e-26)
Length = 800
Score = 28.3 bits (60), Expect = 6.5
Identities = 25/107 (23%), Positives = 50/107 (46%), Gaps = 9/107 (8%)
Query: 54 GAVLLHVLGMGYMFVALAIVCDEFFVPALDVIIER-LEIRDDVAGATFMAAG----GSAP 108
G + V + + VA+ +V + V D +++ ++ VAG + GSA
Sbjct: 610 GDLYYEVSAVAVVVVAVVVVAADIVVVGGDAVVDGGADVVGAVAGGAVIVCNVVIVGSAV 669
Query: 109 ELFTSVIGVFVSFDDVGIG---TIVGSAVFNILFVIGACAMFSRTTL 152
+ ++V+ + V+ + +G IVGS V + + +G CA+ T +
Sbjct: 670 VVVSAVVVIVVAVG-IAVGGSVVIVGSDVVDGVVGVGGCAVAGGTVI 715
>SB_34821| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 380
Score = 27.9 bits (59), Expect = 8.5
Identities = 14/44 (31%), Positives = 23/44 (52%), Gaps = 3/44 (6%)
Query: 178 RDNRIYWQEALVLFSLYGAYVMFMR---WNQPVERAFKKLIYKN 218
R N++YW V+ F+R W++ +ER FK+L K+
Sbjct: 335 RKNKVYWIAGDVVPGKGAVMTCFLREEKWSKSLERLFKRLCKKS 378
>SB_36054| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1267
Score = 27.9 bits (59), Expect = 8.5
Identities = 17/59 (28%), Positives = 29/59 (49%), Gaps = 3/59 (5%)
Query: 140 VIGACAM---FSRTTLSLTWWPLFRDCTFYSISLLVLIYCFRDNRIYWQEALVLFSLYG 195
+IGA A+ F +T SL W L + + V++Y FR+ + + AL+ + G
Sbjct: 1000 IIGAVALPSLFIKTFRSLVWLSLLGTASLLLAFITVIVYEFREYKSWDFRALLFWDFNG 1058
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.328 0.140 0.432
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 7,515,275
Number of Sequences: 59808
Number of extensions: 289157
Number of successful extensions: 1409
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 12
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 1383
Number of HSP's gapped (non-prelim): 24
length of query: 254
length of database: 16,821,457
effective HSP length: 81
effective length of query: 173
effective length of database: 11,977,009
effective search space: 2072022557
effective search space used: 2072022557
T: 11
A: 40
X1: 15 ( 7.1 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.7 bits)
S2: 59 (27.9 bits)
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