GeneModel BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001964-TA|BGIBMGA001964-PA|IPR000886|Endoplasmic
reticulum targeting sequence, IPR002018|Carboxylesterase, type B
         (1028 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC417.12 |||carboxylesterase-lipase family |Schizosaccharomyce...    61   7e-10
SPAPB1A11.02 |||esterase/lipase |Schizosaccharomyces pombe|chr 1...    35   0.067
SPAC8F11.08c |||esterase/lipase|Schizosaccharomyces pombe|chr 1|...    31   0.82 
SPBC1306.01c ||SPBC409.22c|translation elongation factor G|Schiz...    30   1.9  
SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces...    30   1.9  
SPAC139.03 |||transcription factor, zf-fungal binuclear cluster ...    29   3.3  
SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-gluca...    29   3.3  
SPAC1B3.10c |||SEL1 repeat protein, unknown biological role|Schi...    29   4.4  
SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated heterochr...    29   4.4  
SPBC1861.08c |||U2 snRNP-associated protein Lea1 |Schizosaccharo...    28   5.8  
SPCC306.07c |||U3 snoRNP-associated protein Cic1/Utp30 family|Sc...    28   7.7  
SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr...    28   7.7  

>SPCC417.12 |||carboxylesterase-lipase family |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 520

 Score = 61.3 bits (142), Expect = 7e-10
 Identities = 64/228 (28%), Positives = 96/228 (42%), Gaps = 19/228 (8%)

Query: 27  GKVEGLEVKSIIKDEKFYSFLSIPYGKAPIGALRFKAPQPHEGWTDVLNAKKERKQ-CAQ 85
           G + GL V      EK + F  I Y K P+G LR++ P   E   D      + K  C Q
Sbjct: 2   GVLHGLTVLDENGKEKCHRFTGIRYAKPPVGKLRWRRPVTLEDGYDYSGDYNQFKTICPQ 61

Query: 86  YYVPMRP--VDEVGFCGSEDCLHLNIHTXXXXXXXXXXXXVIVFLYNEYFRITYNAS-KE 142
            +   R   V    F   EDCL LNI              V+ F++  + ++      ++
Sbjct: 62  PFYNNRKNQVRNPDFKYDEDCLFLNIWVPAGEKPAEGWP-VLYFIHGGWLQVGNPLHYRQ 120

Query: 143 YGPDFFMKED----VILVTISHRVGTFGFVSFGDDLLP-----GNSGVKDVILALKWIQS 193
             P     +      ILV+  HR+  FGF++ G +LL       N G  D  L L+W   
Sbjct: 121 CDPQDLQADGSPAKFILVSPGHRLNLFGFLA-GKELLEEDPKSSNFGFWDQRLGLEWTYK 179

Query: 194 NINKFGGNPFKITLMGQDGG--AAMVDIL--LHSPKAKGLFSGAILQS 237
           +I  FGGN   I + G   G  +A+  ++   + P+A  +   A+L S
Sbjct: 180 HIESFGGNKENIAVGGISAGSYSALFQLIYETYHPEANQIIKRALLLS 227



 Score = 54.8 bits (126), Expect = 6e-08
 Identities = 47/157 (29%), Positives = 72/157 (45%), Gaps = 20/157 (12%)

Query: 512 WEGTYEAYVEKPTCLQ--FSSRMRNGEEFGISGSEDCLFINIFTPSLKGSA---PVVVFD 566
           + G Y  +  K  C Q  +++R            EDCLF+NI+ P+ +  A   PV+ F 
Sbjct: 48  YSGDYNQF--KTICPQPFYNNRKNQVRNPDFKYDEDCLFLNIWVPAGEKPAEGWPVLYFI 105

Query: 567 YNDNFRTGFNGSLTYA---PDFFVEEG----VVVVTISHRLGLLGYLT----TEDDVIPG 615
           +    + G    L Y    P     +G     ++V+  HRL L G+L      E+D    
Sbjct: 106 HGGWLQVG--NPLHYRQCDPQDLQADGSPAKFILVSPGHRLNLFGFLAGKELLEEDPKSS 163

Query: 616 NNGLRDFLLGLQWIQDNIKSFGGDASRVSVMGNRGGA 652
           N G  D  LGL+W   +I+SFGG+   ++V G   G+
Sbjct: 164 NFGFWDQRLGLEWTYKHIESFGGNKENIAVGGISAGS 200


>SPAPB1A11.02 |||esterase/lipase |Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 339

 Score = 34.7 bits (76), Expect = 0.067
 Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 1/41 (2%)

Query: 183 DVILALKWIQSNINKFGGNPFK-ITLMGQDGGAAMVDILLH 222
           D I + KW+ SNI K G NP +   L G   G   V +L H
Sbjct: 143 DAIDSFKWVASNIEKLGANPKRGFFLGGASAGGNFVSVLSH 183


>SPAC8F11.08c |||esterase/lipase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 376

 Score = 31.1 bits (67), Expect = 0.82
 Identities = 19/59 (32%), Positives = 34/59 (57%), Gaps = 3/59 (5%)

Query: 176 PGNSGVKDVILALKWIQSNINKFGGNPFKITLMGQDGGAAM-VDILLH-SPKA-KGLFS 231
           P +  V+ V L + WI  N   +  +P +I  +G+D GA++ ++ L H  P + KG+F+
Sbjct: 232 PLSDAVEFVSLCVDWIVENAIYYDADPERIFFLGEDTGASVALESLKHIRPNSVKGIFA 290


>SPBC1306.01c ||SPBC409.22c|translation elongation factor
           G|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 770

 Score = 29.9 bits (64), Expect = 1.9
 Identities = 21/90 (23%), Positives = 41/90 (45%), Gaps = 5/90 (5%)

Query: 631 DNIKSFGGDASR-VSVMGNRGGAAIAETLLYSKKAKGLFSAVMIQSGTTLEAMYFYRTPK 689
           +NI     D+ + VS++ +     +A      +   G  + + I  GT     Y Y    
Sbjct: 356 ENIALNAADSEKPVSLVPSSEKPLVALAFKLEEGRFGQLTYLRIYQGTLKRGNYIYNVNS 415

Query: 690 AKAFEFGRVLKIETDDSEVLLEGLQKVEAG 719
            K  +  R++++ ++D    +E ++KVEAG
Sbjct: 416 TKKIKVSRLVRMHSND----MEEIEKVEAG 441


>SPCC191.02c ||SPCC417.14c|acetyl-CoA ligase |Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 662

 Score = 29.9 bits (64), Expect = 1.9
 Identities = 17/56 (30%), Positives = 29/56 (51%), Gaps = 1/56 (1%)

Query: 244 MFFNTKPKDRALAIAEVLERK-LSNSAQFISELGSIAAPKISESELMAIHADDSRK 298
           + F+  PK R+  I   + RK L+     I +L ++A PK+ E  + A+H    +K
Sbjct: 606 LIFSDLPKTRSGKIMRRILRKILAGEVDQIGDLSTLADPKVVEHIIHAVHYAHQKK 661


>SPAC139.03 |||transcription factor, zf-fungal binuclear cluster
           type |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 625

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 13/49 (26%), Positives = 24/49 (48%)

Query: 703 TDDSEVLLEGLQKVEAGELLRNEIGVLDNDEFENDQHSTFPFAPTIEPE 751
           ++  E++ + L +V   E   NE+G     ++EN Q    P  P+ + E
Sbjct: 69  SEADELIDQLLSRVSMLEKRLNEVGTKSQSDYENQQSHNLPSTPSADAE 117


>SPCC1281.01 |ags1|mok1, SPCC338.01c, SPCC17A7.01|alpha-1,4-glucan
           synthase Ags1|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 2410

 Score = 29.1 bits (62), Expect = 3.3
 Identities = 20/57 (35%), Positives = 30/57 (52%), Gaps = 2/57 (3%)

Query: 717 EAGELLRNEIGVLDNDEFENDQHSTFPFAPTIEPENPEAVITNLPENGHIVNDVPVL 773
           ++G  LRN I   D  E E+  +S++   P IE + P A    +PE   I ND P++
Sbjct: 670 KSGTTLRNLIYPYDTIELEDSWNSSWGCIPNIELD-PYAFKLYVPEEDFIEND-PII 724


>SPAC1B3.10c |||SEL1 repeat protein, unknown biological
           role|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 680

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 19/93 (20%), Positives = 42/93 (45%), Gaps = 1/93 (1%)

Query: 796 SHQIEENIVQFPIRQNYRFVNGDKAYKEAAKDILEFYFEDKSVHYGNVMEYTVYAGDVLQ 855
           + Q  +N +   +  +Y F        + A +IL +Y+E + V Y  ++    +A ++L+
Sbjct: 36  AQQYVDNNIGSMVLSDYDFAETPSVRVQRALEILRYYYEQEDVTYEEIIIQRNHAIELLR 95

Query: 856 MYALQKAVTKLSAELQSNLYFYMFDFLGEFNEN 888
             A     T     L +  +F +F+ + E  ++
Sbjct: 96  S-ASHDNNTDAMLYLANIEFFGLFEIIPEIEDS 127


>SPCC1739.03 |hrr1||Helicase Required for RNAi-mediated
           heterochromatin assembly Hrr1|Schizosaccharomyces
           pombe|chr 3|||Manual
          Length = 1015

 Score = 28.7 bits (61), Expect = 4.4
 Identities = 26/104 (25%), Positives = 45/104 (43%), Gaps = 18/104 (17%)

Query: 717 EAGELLRNEIGVLDNDEFENDQHST--------FPFAPTIEPENPEAVITNLPENGHIVN 768
           E   + RN+I    N EFEN+ H           P  PT+E    ++V   LP N     
Sbjct: 100 ELSNVKRNDITSGKNREFENEHHPASDTSSWRELPSIPTLEELTSKSV--ELPSNN---- 153

Query: 769 DVPVLIGFNSREGLDLTSHFLAMPQLVSHQIEENIVQFPIRQNY 812
              +  G+ S E   L+ H+  + +     + E+++++ +  NY
Sbjct: 154 ---IYGGYKSFEDY-LSIHYRLLREDAVSPLRESVLRYKVNPNY 193


>SPBC1861.08c |||U2 snRNP-associated protein Lea1
           |Schizosaccharomyces pombe|chr 2|||Manual
          Length = 239

 Score = 28.3 bits (60), Expect = 5.8
 Identities = 19/51 (37%), Positives = 31/51 (60%), Gaps = 8/51 (15%)

Query: 268 SAQFISELGSIAAPKISESELMAIHADDSRKYQVPILANGPVVEHDHPDAV 318
           +A+F+S++ S  +P + E+EL      D R YQ+PI+ N  V+   H DA+
Sbjct: 4   NAEFLSQVPSFISP-LKETEL------DLRWYQIPIIENLGVLRDVH-DAI 46


>SPCC306.07c |||U3 snoRNP-associated protein Cic1/Utp30
           family|Schizosaccharomyces pombe|chr 3|||Manual
          Length = 284

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 13/42 (30%), Positives = 23/42 (54%)

Query: 936 DVKLLKKMVRLWTNFAKSRNPTPSKDDTVLNGLIWKPVEKEA 977
           D+KLL+K +R      +S +    K+   +    ++PVEKE+
Sbjct: 18  DIKLLEKTIRALLQHIRSSDKPIEKEKVYIQVNTFQPVEKES 59


>SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 872

 Score = 27.9 bits (59), Expect = 7.7
 Identities = 14/40 (35%), Positives = 19/40 (47%)

Query: 930 LPNVLQDVKLLKKMVRLWTNFAKSRNPTPSKDDTVLNGLI 969
           L   LQ ++LL K   +      S  P+PS DD V  G +
Sbjct: 183 LVRALQTIELLLKKYEVSNTTKSSSTPSPSTDDNVPTGTL 222


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.320    0.138    0.407 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,958,359
Number of Sequences: 5004
Number of extensions: 227142
Number of successful extensions: 533
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 520
Number of HSP's gapped (non-prelim): 16
length of query: 1028
length of database: 2,362,478
effective HSP length: 80
effective length of query: 948
effective length of database: 1,962,158
effective search space: 1860125784
effective search space used: 1860125784
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 59 (27.9 bits)