Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001963-TA|BGIBMGA001963-PA|IPR000886|Endoplasmic
reticulum targeting sequence, IPR002018|Carboxylesterase, type B
(409 letters)
Database: bee
429 sequences; 140,377 total letters
Searching.....................................................done
Score E
Sequences producing significant alignments: (bits) Value
AY647436-1|AAU81605.1| 567|Apis mellifera juvenile hormone este... 109 3e-26
AB083009-1|BAC54130.1| 567|Apis mellifera esterase protein. 109 3e-26
AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase ... 63 5e-12
AY526235-1|AAS20468.1| 169|Apis mellifera esterase protein. 62 8e-12
AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase ... 54 2e-09
DQ058012-1|AAY57281.1| 373|Apis mellifera venom allergen acid p... 24 2.0
AY939855-1|AAX33235.1| 388|Apis mellifera venom acid phosphatas... 24 2.0
U15956-1|AAA67444.1| 129|Apis mellifera hymenoptaecin precursor... 23 3.6
DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholi... 22 8.2
DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholi... 22 8.2
>AY647436-1|AAU81605.1| 567|Apis mellifera juvenile hormone
esterase protein.
Length = 567
Score = 109 bits (263), Expect = 3e-26
Identities = 107/419 (25%), Positives = 177/419 (42%), Gaps = 40/419 (9%)
Query: 1 MPGNAGLRDLVLGLEWINGNIEKFGGDRERITLMGSQGGAAAVDLLMHSSAKKLFSAVIL 60
+PGN GL+D + L W++ NIE FGG+ +RITL+G G A+V H SA +
Sbjct: 173 VPGNMGLKDQSMALRWVSENIEWFGGNPKRITLIGLSAGGASV----HYHYLSPLSAGLF 228
Query: 61 QGGSSWSGAYLH-----DNVRERAFNLGEVMNRTASSGHKLIAKLNEVPPDNIVSKDFHA 115
QGG S SG L+ +N E+A +G M + ++I L P IV
Sbjct: 229 QGGISISGTALNCWTQTENSLEKAKQVGAFMGCPTRNVKEMIRCLRYRPARAIVE----- 283
Query: 116 CQKEFSKEYQRSIVTFGPVVEKQPDG-----LITEYPED---SEQQIDMPIMIGYNSCEG 167
F + Y FGPV EK + I P + S D+P + G S EG
Sbjct: 284 TLANFMRFYYNPFTPFGPVTEKVNNDSNSLPFIDRTPIEIINSGDVQDVPWVTGVTSEEG 343
Query: 168 MDTTLYYLLEPRFFSFLHKDFPFILPRRLKFRFDPYKDAFYDAVDEIKSHYF-NNKFNKK 226
+ ++ +P L +++ I P L + + K+ + I+++YF +NK ++
Sbjct: 344 LYPVAEFIAKPEALKLLDENWDLIAPYFLDYNYTIPKEKHVEVARLIRNYYFESNKIDET 403
Query: 227 TIPNFGDYTGDVLTAYSVNCAVSAYAKRSRSPIYYYHFDLFSDFNENKLNIMKMSSVEGV 286
T+ + D D A AK +R P+++Y++ + +I ++ S
Sbjct: 404 TLKHLIDVASDRFFITDGEKAARMQAKVNRQPVWFYYYTY-----KGAHSISEIMSGTSN 458
Query: 287 SWGAAGTDELCYLFKCPNLKETYLKHDNTMSEDRKTQMKIVKLWSNFAKYRNPTPDNDET 346
+G D+ + P L T T + D K Q ++ W +F N P N +
Sbjct: 459 KYGVCHADDAYMVVDTPFLAST------TTTNDIKMQKVLIDFWVSFV--NNGVP-NVNS 509
Query: 347 LAGLRWSPYTVENEEYLHM--DNDFKMKTELNKSKFKFWDDFISRWEKKAVNGVISEPL 403
+ R +P ++ YLH+ +M + N + FW+ K + + E L
Sbjct: 510 VQWPRLNP-NEKSLHYLHIAGPGKIQMDSSTNFGREDFWNSINFNENKLHTSDTLKEEL 567
>AB083009-1|BAC54130.1| 567|Apis mellifera esterase protein.
Length = 567
Score = 109 bits (263), Expect = 3e-26
Identities = 107/419 (25%), Positives = 177/419 (42%), Gaps = 40/419 (9%)
Query: 1 MPGNAGLRDLVLGLEWINGNIEKFGGDRERITLMGSQGGAAAVDLLMHSSAKKLFSAVIL 60
+PGN GL+D + L W++ NIE FGG+ +RITL+G G A+V H SA +
Sbjct: 173 VPGNMGLKDQSMALRWVSENIEWFGGNPKRITLIGLSAGGASV----HYHYLSPLSAGLF 228
Query: 61 QGGSSWSGAYLH-----DNVRERAFNLGEVMNRTASSGHKLIAKLNEVPPDNIVSKDFHA 115
QGG S SG L+ +N E+A +G M + ++I L P IV
Sbjct: 229 QGGISISGTALNCWTQTENSLEKAKQVGAFMGCPTRNVKEMIRCLRYRPARAIVE----- 283
Query: 116 CQKEFSKEYQRSIVTFGPVVEKQPDG-----LITEYPED---SEQQIDMPIMIGYNSCEG 167
F + Y FGPV EK + I P + S D+P + G S EG
Sbjct: 284 TLANFMRFYYNPFTPFGPVTEKVNNDSNSLPFIDRTPIEIINSGDVQDVPWVTGVTSEEG 343
Query: 168 MDTTLYYLLEPRFFSFLHKDFPFILPRRLKFRFDPYKDAFYDAVDEIKSHYF-NNKFNKK 226
+ ++ +P L +++ I P L + + K+ + I+++YF +NK ++
Sbjct: 344 LYPVAEFIAKPEALKLLDENWDLIAPYFLDYNYTIPKEKHVEVARLIRNYYFESNKIDET 403
Query: 227 TIPNFGDYTGDVLTAYSVNCAVSAYAKRSRSPIYYYHFDLFSDFNENKLNIMKMSSVEGV 286
T+ + D D A AK +R P+++Y++ + +I ++ S
Sbjct: 404 TLKHLIDVASDRFFITDGEKAARMQAKVNRQPVWFYYYTY-----KGAHSISEIMSGTSN 458
Query: 287 SWGAAGTDELCYLFKCPNLKETYLKHDNTMSEDRKTQMKIVKLWSNFAKYRNPTPDNDET 346
+G D+ + P L T T + D K Q ++ W +F N P N +
Sbjct: 459 KYGVCHADDAYMVVDTPFLAST------TTTNDIKMQKVLIDFWVSFV--NNGVP-NVNS 509
Query: 347 LAGLRWSPYTVENEEYLHM--DNDFKMKTELNKSKFKFWDDFISRWEKKAVNGVISEPL 403
+ R +P ++ YLH+ +M + N + FW+ K + + E L
Sbjct: 510 VQWPRLNP-NEKSLHYLHIAGPGKIQMDSSTNFGREDFWNSINFNENKLHTSDTLKEEL 567
>AB181702-1|BAE06051.1| 628|Apis mellifera acetylcholinesterase
protein.
Length = 628
Score = 62.9 bits (146), Expect = 5e-12
Identities = 101/404 (25%), Positives = 163/404 (40%), Gaps = 54/404 (13%)
Query: 2 PGNAGLRDLVLGLEWINGNIEKFGGDRERITLMGSQGGAAAVDL-LMHSSAKKLFSAVIL 60
PGN GL D L L W+ N E FGGD E IT+ G G ++V L L+ + L IL
Sbjct: 218 PGNMGLWDQALALRWLRDNAEAFGGDPELITIFGESAGGSSVSLHLISPVTRGLVRRGIL 277
Query: 61 QGG---SSWSGAYLHDNVRERAFNLGEVMNRTASSGHKLIAKLNEVPPDNIVSKDFHACQ 117
Q G + WS Y+ E+A + ++ + LNE P + +
Sbjct: 278 QSGTLNAPWS--YMSG---EKANEVATILVDDCGCNSTM---LNENPARVMACMRSVDAK 329
Query: 118 KEFSKEYQRSIVTFGPVVEKQPDGL-ITEYPEDSEQQIDM---PIMIGYNSCEGMDTTLY 173
+++ G DG+ + ++P D ++ D I+IG N EG LY
Sbjct: 330 TISVQQWNSYWGILGFPSAPTIDGIFLPKHPLDLLREADFKDTEILIGNNENEGTYFILY 389
Query: 174 ---YLLEPRFFSFLHKD-FPFILPRRLKFRFDPYKDAFYDAVDEIKSHYFNNKFNKKTIP 229
+ E SFL ++ F I+ K ++A + + Y + K
Sbjct: 390 DFNDIFEKDQASFLERERFLGIINNIFKNMSQIEREAITFQYTDWEEVYNGYIYQKMVAD 449
Query: 230 NFGDYTGDVLTAYSVNCAVSAYAKRSRSPIYYYHFDLFSDFNENKLNIMKMSSVEGVSWG 289
GDY S++ A +A R +YYY F + N + G G
Sbjct: 450 VVGDY---FFICPSIHFA-QLFADRGMK-VYYYFFTQRTSTN-----------LWGEWMG 493
Query: 290 AAGTDELCYLFKCPNLKETYLKHDNTMSEDRKTQMKIVKLWSNFAKYRNPTPDNDETLAG 349
DE+ Y+F P K LK+ + ++R ++++ +S FA PT ++ E
Sbjct: 494 VLHGDEVEYVFGHPLNKS--LKYSD---KERDLSLRMILYFSEFAYLGKPTKEDSE---- 544
Query: 350 LRWSPYTVENEEYLHMDNDFKMKTELNK----SKFKFWDDFISR 389
W Y+ + +Y D + KT L K + FW++F+ +
Sbjct: 545 --WPSYSRDEPKYFIFDAE---KTGLGKGPRTTYCAFWNEFLPK 583
>AY526235-1|AAS20468.1| 169|Apis mellifera esterase protein.
Length = 169
Score = 62.1 bits (144), Expect = 8e-12
Identities = 42/114 (36%), Positives = 59/114 (51%), Gaps = 9/114 (7%)
Query: 1 MPGNAGLRDLVLGLEWINGNIEKFGGDRERITLMGSQGGAAAVDLLMHSSAKKLFSAVIL 60
+PGN GL+D + L W++ NIE FGG+ +RITL+G G A+V H SA +
Sbjct: 44 VPGNMGLKDQSMALRWVSENIEWFGGNPKRITLIGLSAGGASV----HYHYLSPLSAGLF 99
Query: 61 QGGSSWSGAYLH-----DNVRERAFNLGEVMNRTASSGHKLIAKLNEVPPDNIV 109
QGG S SG L+ +N E+A +G M + ++I L P IV
Sbjct: 100 QGGISISGTALNCWTQTENSLEKAKQVGAFMGCPTRNVKEMIRCLRYRPARAIV 153
>AF213012-1|AAG43568.1| 492|Apis mellifera acetylcholinesterase
protein.
Length = 492
Score = 54.0 bits (124), Expect = 2e-09
Identities = 28/64 (43%), Positives = 35/64 (54%), Gaps = 1/64 (1%)
Query: 2 PGNAGLRDLVLGLEWINGNIEKFGGDRERITLMGSQGGAAAVDL-LMHSSAKKLFSAVIL 60
PGN GL D L L W+ N E FGGD E IT+ G G ++V L L+ + L IL
Sbjct: 218 PGNMGLWDQALALRWLRDNAEAFGGDPELITIFGESAGGSSVSLHLISPVTRGLVRRGIL 277
Query: 61 QGGS 64
Q G+
Sbjct: 278 QSGT 281
>DQ058012-1|AAY57281.1| 373|Apis mellifera venom allergen acid
phosphatase protein.
Length = 373
Score = 24.2 bits (50), Expect = 2.0
Identities = 10/27 (37%), Positives = 15/27 (55%)
Query: 231 FGDYTGDVLTAYSVNCAVSAYAKRSRS 257
+GD+ GD+ T SV+ S Y + S
Sbjct: 60 YGDFLGDIYTEESVSALSSFYDRTKMS 86
>AY939855-1|AAX33235.1| 388|Apis mellifera venom acid phosphatase
precursor protein.
Length = 388
Score = 24.2 bits (50), Expect = 2.0
Identities = 10/27 (37%), Positives = 15/27 (55%)
Query: 231 FGDYTGDVLTAYSVNCAVSAYAKRSRS 257
+GD+ GD+ T SV+ S Y + S
Sbjct: 75 YGDFLGDIYTEESVSALSSFYDRTKMS 101
>U15956-1|AAA67444.1| 129|Apis mellifera hymenoptaecin precursor
protein.
Length = 129
Score = 23.4 bits (48), Expect = 3.6
Identities = 9/28 (32%), Positives = 15/28 (53%)
Query: 119 EFSKEYQRSIVTFGPVVEKQPDGLITEY 146
EF KEY+ + V++ P G ++ Y
Sbjct: 93 EFGKEYKNGFIKGQSEVQRGPGGRLSPY 120
>DQ026034-1|AAY87893.1| 569|Apis mellifera nicotinic acetylcholine
receptor alpha4subunit protein.
Length = 569
Score = 22.2 bits (45), Expect = 8.2
Identities = 8/16 (50%), Positives = 9/16 (56%)
Query: 383 WDDFISRWEKKAVNGV 398
W D+ RWE K GV
Sbjct: 85 WYDYKLRWEPKEYGGV 100
>DQ026033-1|AAY87892.1| 569|Apis mellifera nicotinic acetylcholine
receptor alpha4subunit protein.
Length = 569
Score = 22.2 bits (45), Expect = 8.2
Identities = 8/16 (50%), Positives = 9/16 (56%)
Query: 383 WDDFISRWEKKAVNGV 398
W D+ RWE K GV
Sbjct: 85 WYDYKLRWEPKEYGGV 100
Database: bee
Posted date: Oct 5, 2007 11:13 AM
Number of letters in database: 140,377
Number of sequences in database: 429
Lambda K H
0.318 0.136 0.414
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 136,555
Number of Sequences: 429
Number of extensions: 6299
Number of successful extensions: 40
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 22
Number of HSP's gapped (non-prelim): 10
length of query: 409
length of database: 140,377
effective HSP length: 59
effective length of query: 350
effective length of database: 115,066
effective search space: 40273100
effective search space used: 40273100
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 45 (22.2 bits)
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