Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001918-TA|BGIBMGA001918-PA|IPR001096|Peptidase C13,
legumain
(264 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At1g08750.3 68414.m00974 GPI-anchor transamidase, putative simil... 256 1e-68
At1g08750.2 68414.m00973 GPI-anchor transamidase, putative simil... 256 1e-68
At1g08750.1 68414.m00972 GPI-anchor transamidase, putative simil... 256 1e-68
At3g20210.1 68416.m02561 vacuolar processing enzyme, putative / ... 75 7e-14
At2g25940.1 68415.m03113 vacuolar processing enzyme alpha / alph... 74 1e-13
At4g32940.1 68417.m04687 vacuolar processing enzyme gamma / gamm... 70 2e-12
At1g62710.1 68414.m07078 vacuolar processing enzyme beta / beta-... 61 9e-10
At5g10890.1 68418.m01264 myosin heavy chain-related contains wea... 34 0.085
At3g05675.2 68416.m00633 expressed protein 33 0.15
At3g05675.1 68416.m00632 expressed protein 33 0.15
At1g22275.1 68414.m02784 expressed protein 29 3.2
At1g63550.1 68414.m07184 hypothetical protein low similarity to ... 29 4.2
At1g75250.1 68414.m08742 myb family transcription factor contain... 28 5.6
At1g22260.1 68414.m02782 expressed protein 28 7.4
At5g54010.1 68418.m06718 glycosyltransferase family protein cont... 27 9.8
At4g20800.1 68417.m03019 FAD-binding domain-containing protein s... 27 9.8
>At1g08750.3 68414.m00974 GPI-anchor transamidase, putative similar
to SP|P49018 GPI-anchor transamidase (EC 3.-.-.-) (GPI
transamidase) {Saccharomyces cerevisiae}; contains Pfam
profile PF01650: Peptidase C13 family
Length = 388
Score = 256 bits (626), Expect = 1e-68
Identities = 116/224 (51%), Positives = 163/224 (72%)
Query: 1 MACNPRNPRPATIFNSAHEQINVYGDDVEVDYRGYEVSVENFIRLLTGRVPPDTPRSKQL 60
MACN RN PA +FN+ + ++N+YGD+VEVDYRGYEV+VENF+R+LTGR PRSK+L
Sbjct: 72 MACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENAVPRSKRL 131
Query: 61 LTDEGSNILIYLTGHGGDGFLKFQDSEEVTSQELADALEQMWQKKRYNEIFFIIDTCQAS 120
L+DEGS+IL+Y+TGHGGD FLKFQD+EE+ S +LADA++QM +K+R+ E+ ++DTCQA+
Sbjct: 132 LSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMVDTCQAA 191
Query: 121 SMYEKFYSPNILATASSLVGEDSLSHHVDSAIGVYIIDRYTYYVLEFLENVHPNSKRTMS 180
+++ + SP +LA SSL GE+S SHH+DS IGV ++DR+TYY L F E ++ +++
Sbjct: 192 TLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFERLNIYDNASLN 251
Query: 181 EFLAVCPKSACLSTVGVRKDLFNRDPSKVPITDFFGSVRPVILT 224
+ST R DL+ +VP+T+FFGSV I T
Sbjct: 252 SLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHT 295
>At1g08750.2 68414.m00973 GPI-anchor transamidase, putative similar
to SP|P49018 GPI-anchor transamidase (EC 3.-.-.-) (GPI
transamidase) {Saccharomyces cerevisiae}; contains Pfam
profile PF01650: Peptidase C13 family
Length = 388
Score = 256 bits (626), Expect = 1e-68
Identities = 116/224 (51%), Positives = 163/224 (72%)
Query: 1 MACNPRNPRPATIFNSAHEQINVYGDDVEVDYRGYEVSVENFIRLLTGRVPPDTPRSKQL 60
MACN RN PA +FN+ + ++N+YGD+VEVDYRGYEV+VENF+R+LTGR PRSK+L
Sbjct: 72 MACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENAVPRSKRL 131
Query: 61 LTDEGSNILIYLTGHGGDGFLKFQDSEEVTSQELADALEQMWQKKRYNEIFFIIDTCQAS 120
L+DEGS+IL+Y+TGHGGD FLKFQD+EE+ S +LADA++QM +K+R+ E+ ++DTCQA+
Sbjct: 132 LSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMVDTCQAA 191
Query: 121 SMYEKFYSPNILATASSLVGEDSLSHHVDSAIGVYIIDRYTYYVLEFLENVHPNSKRTMS 180
+++ + SP +LA SSL GE+S SHH+DS IGV ++DR+TYY L F E ++ +++
Sbjct: 192 TLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFERLNIYDNASLN 251
Query: 181 EFLAVCPKSACLSTVGVRKDLFNRDPSKVPITDFFGSVRPVILT 224
+ST R DL+ +VP+T+FFGSV I T
Sbjct: 252 SLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHT 295
>At1g08750.1 68414.m00972 GPI-anchor transamidase, putative similar
to SP|P49018 GPI-anchor transamidase (EC 3.-.-.-) (GPI
transamidase) {Saccharomyces cerevisiae}; contains Pfam
profile PF01650: Peptidase C13 family
Length = 388
Score = 256 bits (626), Expect = 1e-68
Identities = 116/224 (51%), Positives = 163/224 (72%)
Query: 1 MACNPRNPRPATIFNSAHEQINVYGDDVEVDYRGYEVSVENFIRLLTGRVPPDTPRSKQL 60
MACN RN PA +FN+ + ++N+YGD+VEVDYRGYEV+VENF+R+LTGR PRSK+L
Sbjct: 72 MACNARNEYPAQVFNNENHKLNLYGDNVEVDYRGYEVTVENFLRVLTGRHENAVPRSKRL 131
Query: 61 LTDEGSNILIYLTGHGGDGFLKFQDSEEVTSQELADALEQMWQKKRYNEIFFIIDTCQAS 120
L+DEGS+IL+Y+TGHGGD FLKFQD+EE+ S +LADA++QM +K+R+ E+ ++DTCQA+
Sbjct: 132 LSDEGSHILLYMTGHGGDEFLKFQDAEELQSHDLADAVKQMKEKRRFKELMIMVDTCQAA 191
Query: 121 SMYEKFYSPNILATASSLVGEDSLSHHVDSAIGVYIIDRYTYYVLEFLENVHPNSKRTMS 180
+++ + SP +LA SSL GE+S SHH+DS IGV ++DR+TYY L F E ++ +++
Sbjct: 192 TLFNQLQSPGVLAIGSSLKGENSYSHHLDSDIGVSVVDRFTYYTLAFFERLNIYDNASLN 251
Query: 181 EFLAVCPKSACLSTVGVRKDLFNRDPSKVPITDFFGSVRPVILT 224
+ST R DL+ +VP+T+FFGSV I T
Sbjct: 252 SLFRSYDPRLLMSTAYYRTDLYQPHLVEVPVTNFFGSVMETIHT 295
>At3g20210.1 68416.m02561 vacuolar processing enzyme, putative /
asparaginyl endopeptidase, putative similar to
asparaginyl endopeptidase (VmPE-1) [Vigna mungo]
GI:4589396; contains Pfam profile PF01650: Peptidase C13
family; identical to cDNA vacuolar processing enzyme
delta preproprotein (At3g20210) GI:24850432
Length = 466
Score = 74.5 bits (175), Expect = 7e-14
Identities = 53/191 (27%), Positives = 88/191 (46%), Gaps = 13/191 (6%)
Query: 1 MACNPRNPRPATIFNSAHEQINVYGDDVEVDYRGYEVSVENFIRLLTGRVPPDTP-RSKQ 59
+A + NPRP I N + +VY V DY V+V+NF +L G T K
Sbjct: 91 IAFSSENPRPGVIINKPDGE-DVY-KGVPKDYTKEAVNVQNFYNVLLGNESGVTGGNGKV 148
Query: 60 LLTDEGSNILIYLTGHGGDGFLKFQDSEEVTSQELADALEQMWQKKRYNEIFFIIDTCQA 119
+ + NI IY HG G + +EV +++ + LE+M ++K+YN++ ++ C++
Sbjct: 149 VKSGPNDNIFIYYADHGAPGLIAMPTGDEVMAKDFNEVLEKMHKRKKYNKMVIYVEACES 208
Query: 120 SSMYEKFYSPNILATASSLVGEDSLSHHV---------DSAIGVYIIDRYTYYVLEFLEN 170
SM+E N+ A + S V S IG + D ++ LE +
Sbjct: 209 GSMFEGILKKNLNIYAVTAANSKESSWGVYCPESYPPPPSEIGTCLGDTFSISWLED-SD 267
Query: 171 VHPNSKRTMSE 181
+H SK T+ +
Sbjct: 268 LHDMSKETLEQ 278
>At2g25940.1 68415.m03113 vacuolar processing enzyme alpha /
alpha-VPE identical to SP|P49047 Vacuolar processing
enzyme, alpha-isozyme precursor (EC 3.4.22.-)
(Alpha-VPE) {Arabidopsis thaliana}
Length = 478
Score = 73.7 bits (173), Expect = 1e-13
Identities = 48/147 (32%), Positives = 76/147 (51%), Gaps = 7/147 (4%)
Query: 1 MACNPRNPRPATIFNSAHEQINVYGDDVEVDYRGYEVSVENFIRLLTGRVPPDTPRSKQL 60
+A N NPRP I NS + + +VY + V DY G EV+V+N + ++ G S ++
Sbjct: 89 IAKNEENPRPGVIINSPNGE-DVY-NGVPKDYTGDEVNVDNLLAVILGNKTALKGGSGKV 146
Query: 61 LTDEGSN--ILIYLTGHGGDGFLKFQDSEEVTSQELADALEQMWQKKRYNEIFFIIDTCQ 118
+ D G N I IY + HGG G L S + + +L D L++ + Y + F ++ C+
Sbjct: 147 V-DSGPNDHIFIYYSDHGGPGVLGMPTSPNLYANDLNDVLKKKYASGTYKSLVFYLEACE 205
Query: 119 ASSMYEKFYSP--NILATASSLVGEDS 143
+ S++E NI AT +S E S
Sbjct: 206 SGSIFEGLLPEGLNIYATTASNAEESS 232
>At4g32940.1 68417.m04687 vacuolar processing enzyme gamma /
gamma-VPE nearly identical to SP|Q39119 Vacuolar
processing enzyme, gamma-isozyme precursor (EC 3.4.22.-)
(Gamma-VPE) {Arabidopsis thaliana}
Length = 494
Score = 69.7 bits (163), Expect = 2e-12
Identities = 48/147 (32%), Positives = 74/147 (50%), Gaps = 7/147 (4%)
Query: 1 MACNPRNPRPATIFNSAHEQINVYGDDVEVDYRGYEVSVENFIRLLTGRVPPDTPRSKQL 60
+A N NPRP TI NS H + +VY V DY G +V+V+N ++ G S ++
Sbjct: 104 IANNYENPRPGTIINSPHGK-DVY-QGVPKDYTGDDVNVDNLFAVILGDKTAVKGGSGKV 161
Query: 61 LTDEGSN--ILIYLTGHGGDGFLKFQDSEEVTSQELADALEQMWQKKRYNEIFFIIDTCQ 118
+ D G N I I+ + HGG G L S + + +L D L++ Y + F ++ C+
Sbjct: 162 V-DSGPNDHIFIFYSDHGGPGVLGMPTSPYLYANDLNDVLKKKHALGTYKSLVFYLEACE 220
Query: 119 ASSMYEKFYSP--NILATASSLVGEDS 143
+ S++E NI AT +S E S
Sbjct: 221 SGSIFEGLLPEGLNIYATTASNAEESS 247
>At1g62710.1 68414.m07078 vacuolar processing enzyme beta / beta-VPE
identical to SP|Q39044 Vacuolar processing enzyme,
beta-isozyme precursor (EC 3.4.22.-) (Beta-VPE)
{Arabidopsis thaliana}
Length = 486
Score = 60.9 bits (141), Expect = 9e-10
Identities = 39/146 (26%), Positives = 67/146 (45%), Gaps = 5/146 (3%)
Query: 1 MACNPRNPRPATIFNSAHEQINVYGDDVEVDYRGYEVSVENFIRLLTGRVPPDTPRSKQL 60
+A +P NPRP T+ N +VY V DY G V+ NF +L G S ++
Sbjct: 96 IANHPLNPRPGTLINHPDGD-DVYAG-VPKDYTGSSVTAANFYAVLLGDQKAVKGGSGKV 153
Query: 61 LTDE-GSNILIYLTGHGGDGFLKFQDSEEVTSQELADALEQMWQKKRYNEIFFIIDTCQA 119
+ + +I +Y HGG G L ++ + + + + L++ Y E+ ++ C++
Sbjct: 154 IASKPNDHIFVYYADHGGPGVLGMPNTPHIYAADFIETLKKKHASGTYKEMVIYVEACES 213
Query: 120 SSMYEKFYSP--NILATASSLVGEDS 143
S++E NI T +S E S
Sbjct: 214 GSIFEGIMPKDLNIYVTTASNAQESS 239
>At5g10890.1 68418.m01264 myosin heavy chain-related contains weak
similarity to myosin heavy chain [Rana catesbeiana]
gi|4249703|gb|AAD13773
Length = 295
Score = 34.3 bits (75), Expect = 0.085
Identities = 19/61 (31%), Positives = 34/61 (55%), Gaps = 2/61 (3%)
Query: 88 EVTSQELADALEQMWQKKRYNEIFFIIDTCQASSMYEKFYSPNILATASSLVGEDSLSHH 147
E + EL + + Y+ ++D ++S+ + +YSP I+ T+ S+ GE SLSH+
Sbjct: 106 EFHNNELRRKQLEKTETSAYSATSDVVDGPESST--DHYYSPQIIQTSMSVGGEGSLSHY 163
Query: 148 V 148
V
Sbjct: 164 V 164
>At3g05675.2 68416.m00633 expressed protein
Length = 441
Score = 33.5 bits (73), Expect = 0.15
Identities = 20/65 (30%), Positives = 30/65 (46%), Gaps = 1/65 (1%)
Query: 91 SQELADALEQMWQKKRYNEIFFIIDT-CQASSMYEKFYSPNILATASSLVGEDSLSHHVD 149
SQ AD L W + + + ++ CQ S +L S V ED+L H+V
Sbjct: 47 SQYFADRLSDKWPTCKILDSRYCVEVICQESDYDHHINLLRLLYVVSDDVHEDNLCHNVK 106
Query: 150 SAIGV 154
SA+G+
Sbjct: 107 SALGI 111
>At3g05675.1 68416.m00632 expressed protein
Length = 441
Score = 33.5 bits (73), Expect = 0.15
Identities = 20/65 (30%), Positives = 30/65 (46%), Gaps = 1/65 (1%)
Query: 91 SQELADALEQMWQKKRYNEIFFIIDT-CQASSMYEKFYSPNILATASSLVGEDSLSHHVD 149
SQ AD L W + + + ++ CQ S +L S V ED+L H+V
Sbjct: 47 SQYFADRLSDKWPTCKILDSRYCVEVICQESDYDHHINLLRLLYVVSDDVHEDNLCHNVK 106
Query: 150 SAIGV 154
SA+G+
Sbjct: 107 SALGI 111
>At1g22275.1 68414.m02784 expressed protein
Length = 856
Score = 29.1 bits (62), Expect = 3.2
Identities = 13/39 (33%), Positives = 26/39 (66%), Gaps = 2/39 (5%)
Query: 87 EEVTSQELADALEQMWQKKRYNEIFFIIDTCQASSMYEK 125
EE+TS++ LE++ +K++ E+F+ + C +S+ EK
Sbjct: 172 EEITSRD--KELEELKLEKQHKEMFYQTERCGTASLIEK 208
>At1g63550.1 68414.m07184 hypothetical protein low similarity to
receptor-like protein kinase 5 [Arabidopsis thaliana]
GI:13506747; contains Pfam profile: PF01657 Domain of
unknown function DUF26
Length = 299
Score = 28.7 bits (61), Expect = 4.2
Identities = 31/124 (25%), Positives = 51/124 (41%), Gaps = 15/124 (12%)
Query: 23 VYGDDVEVDYRGY----EVSVENFIRLLTGRVPPDTPRSKQLLTDEGSNILIYLTGHGGD 78
+Y D+ V Y E+ + I + + R P+ R Q LT++ S ++ ++
Sbjct: 93 IYYDECMVRYSNVSFSSELEIVPSITIYSLRSAPNPTRFNQTLTEKFSELIFNVSSSSLV 152
Query: 79 GFLKFQDSEEVTSQELADALEQMWQKKRYNEIF----------FIIDTCQASSMYEKFYS 128
+ +D E VT E + L+ M Q +IF F I TC Y K ++
Sbjct: 153 PYF-VEDQERVTQSEGSYDLDTMVQCSPDLDIFNCTVCLRVAFFRISTCCGLPSYAKIFT 211
Query: 129 PNIL 132
P L
Sbjct: 212 PKCL 215
>At1g75250.1 68414.m08742 myb family transcription factor contains
PFAM profile: PF00249 myb-like DNA binding domain
Length = 126
Score = 28.3 bits (60), Expect = 5.6
Identities = 14/29 (48%), Positives = 18/29 (62%)
Query: 23 VYGDDVEVDYRGYEVSVENFIRLLTGRVP 51
V G VE R Y++ VE+ I + TGRVP
Sbjct: 42 VGGKTVEEVKRHYDILVEDLINIETGRVP 70
>At1g22260.1 68414.m02782 expressed protein
Length = 857
Score = 27.9 bits (59), Expect = 7.4
Identities = 13/39 (33%), Positives = 25/39 (64%), Gaps = 2/39 (5%)
Query: 87 EEVTSQELADALEQMWQKKRYNEIFFIIDTCQASSMYEK 125
EE+TS++ LE++ +K+ E+F+ + C +S+ EK
Sbjct: 172 EEITSRD--KELEELKLEKQQKEMFYQTERCGTASLIEK 208
>At5g54010.1 68418.m06718 glycosyltransferase family protein
contains Pfam profile: PF00201 UDP-glucoronosyl and
UDP-glucosyl transferase
Length = 453
Score = 27.5 bits (58), Expect = 9.8
Identities = 14/32 (43%), Positives = 17/32 (53%)
Query: 48 GRVPPDTPRSKQLLTDEGSNILIYLTGHGGDG 79
G PP P SK LL +N L +L+ GDG
Sbjct: 155 GSTPPGYPSSKVLLRGHETNSLSFLSYPFGDG 186
>At4g20800.1 68417.m03019 FAD-binding domain-containing protein
similar to SP|P93479 Reticuline oxidase precursor (EC
1.5.3.9) (Berberine-bridge-forming enzyme) {Papaver
somniferum}; contains Pfam profile PF01565: FAD binding
domain
Length = 528
Score = 27.5 bits (58), Expect = 9.8
Identities = 11/29 (37%), Positives = 17/29 (58%)
Query: 38 SVENFIRLLTGRVPPDTPRSKQLLTDEGS 66
++ENF+R L R P P ++ + T E S
Sbjct: 25 NIENFLRCLRNRTNPKNPIAEAIYTHENS 53
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.320 0.137 0.405
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 6,089,904
Number of Sequences: 28952
Number of extensions: 246018
Number of successful extensions: 588
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 571
Number of HSP's gapped (non-prelim): 16
length of query: 264
length of database: 12,070,560
effective HSP length: 80
effective length of query: 184
effective length of database: 9,754,400
effective search space: 1794809600
effective search space used: 1794809600
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 58 (27.5 bits)
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