Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001892-TA|BGIBMGA001892-PA|IPR000834|Peptidase M14,
carboxypeptidase A
(298 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_37943| Best HMM Match : Peptidase_M14 (HMM E-Value=0) 80 2e-15
SB_49100| Best HMM Match : Peptidase_M14 (HMM E-Value=0) 77 2e-14
SB_24088| Best HMM Match : No HMM Matches (HMM E-Value=.) 69 5e-12
SB_15763| Best HMM Match : No HMM Matches (HMM E-Value=.) 55 6e-08
SB_62| Best HMM Match : Peptidase_M14 (HMM E-Value=2.7e-34) 48 1e-05
SB_49098| Best HMM Match : Peptidase_M14 (HMM E-Value=4.5e-12) 38 0.010
SB_1170| Best HMM Match : No HMM Matches (HMM E-Value=.) 34 0.16
SB_34368| Best HMM Match : No HMM Matches (HMM E-Value=.) 33 0.28
SB_41552| Best HMM Match : RVT_1 (HMM E-Value=5.4e-32) 32 0.65
SB_37941| Best HMM Match : Peptidase_M14 (HMM E-Value=0) 31 0.86
SB_51769| Best HMM Match : No HMM Matches (HMM E-Value=.) 31 1.1
SB_28263| Best HMM Match : Peptidase_M14 (HMM E-Value=0) 31 1.5
SB_23547| Best HMM Match : NLPC_P60 (HMM E-Value=2.5) 30 2.6
SB_50158| Best HMM Match : Toxin_16 (HMM E-Value=2) 29 3.5
SB_15157| Best HMM Match : DUF1153 (HMM E-Value=1.7) 29 3.5
SB_27034| Best HMM Match : No HMM Matches (HMM E-Value=.) 29 4.6
SB_18998| Best HMM Match : Exo_endo_phos (HMM E-Value=1.1e-10) 29 6.1
SB_13109| Best HMM Match : Dynein_heavy (HMM E-Value=2.6e-09) 29 6.1
SB_59792| Best HMM Match : RVT_1 (HMM E-Value=4.2039e-45) 28 8.0
SB_24812| Best HMM Match : No HMM Matches (HMM E-Value=.) 28 8.0
SB_53466| Best HMM Match : SCP (HMM E-Value=2.4e-27) 28 8.0
SB_6367| Best HMM Match : Seryl_tRNA_N (HMM E-Value=0.84) 28 8.0
>SB_37943| Best HMM Match : Peptidase_M14 (HMM E-Value=0)
Length = 249
Score = 79.8 bits (188), Expect = 2e-15
Identities = 59/191 (30%), Positives = 88/191 (46%), Gaps = 11/191 (5%)
Query: 26 INKEVEKAKEDLKPVIIIDAGQQGGK-ESVMLALYVIEQLVACNDN----SEMIEKVKWV 80
IN K K D KPV ++ G + S +Y+I +LV+ + + +++K+ ++
Sbjct: 58 INGTNGKYKAD-KPVFFVNCGIHAREWVSPATCMYIIHELVSKYEKDAKVTSVLDKMDFI 116
Query: 81 VLPSTNPDGMEFARYLMP--YWKKNLKPLDDGPRLGVDISRNFDKEWNSCPEVENKFSQI 138
+ P NPDG F W+K+ KP +G D++RNF +W + S I
Sbjct: 117 IHPMVNPDGYVFTHKNESNRLWRKSRKPTSKAGCIGADLNRNFGYQWGKPGSSDKPCSFI 176
Query: 139 YPGAAAASENETIFIQNALKKYQKNAKIYLSIRRDGHSIAYPYGYSKTAPSNEAKLKRVA 198
Y G A SE E + L K N K YL I G YP+GY+ + E K +
Sbjct: 177 YRGEAPYSEVEVKNMAAFLWKLAPNLKGYLDIHSYGQLWMYPWGYTNQSTQVE---KEMD 233
Query: 199 SEIATRVNQKT 209
+ ATR NQ T
Sbjct: 234 THGATRANQPT 244
>SB_49100| Best HMM Match : Peptidase_M14 (HMM E-Value=0)
Length = 313
Score = 77.0 bits (181), Expect = 2e-14
Identities = 53/174 (30%), Positives = 84/174 (48%), Gaps = 8/174 (4%)
Query: 56 LALYVIEQLVACNDNSEMIEKVKWVVLPSTNPDGMEFARYLMPYWKKNLKPLDDGPR-LG 114
LA+ ++E + +D ++M++ +WV+LP N DG E+ R W+K P G + +G
Sbjct: 90 LAIKILETGDSDSDVTDMLDNYEWVILPVLNVDGYEYTRTKDRMWRKTRTP---GTKCVG 146
Query: 115 VDISRNFDKEWNSCPEVENKFSQIYPGAAAASENETIFIQNALKKYQKNAKIYLSIRRDG 174
VD +RNF+ + N+ SQIY G A SE T + L + + K Y+
Sbjct: 147 VDPNRNFNYRFGVTGTSNNECSQIYRGKQAFSEKVTYNVAKFLYENRHRLKGYIDFHAYS 206
Query: 175 HSIAYPYGYSKTAPSNEAKLKRVASEIATRVNQKTNGI-YLF--VNTSIYELEG 225
P+GY+ PSN + + A A Q +G Y+F +T+IY G
Sbjct: 207 QMFLSPWGYTDAKPSNYTEHMK-AMTAAVNALQAVHGTRYVFGPASTTIYPTAG 259
>SB_24088| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 245
Score = 68.9 bits (161), Expect = 5e-12
Identities = 44/164 (26%), Positives = 78/164 (47%), Gaps = 5/164 (3%)
Query: 38 KPVIIIDAGQQGGK-ESVMLALYVIEQLVACNDN----SEMIEKVKWVVLPSTNPDGMEF 92
KPV ++ G + S +Y+ +QL++ + +++K+ +V++P NPDG F
Sbjct: 9 KPVFFMNCGIHAREWVSPATCMYIAKQLLSRYGKDANVTSVLDKMDFVIMPVLNPDGYVF 68
Query: 93 ARYLMPYWKKNLKPLDDGPRLGVDISRNFDKEWNSCPEVENKFSQIYPGAAAASENETIF 152
+W+K LKP +G D +RN+D +W + + + Y G A SE E +
Sbjct: 69 TWERQRFWRKTLKPNSGTFCVGTDPNRNWDYKWGAEGASTDPCADDYRGPHAFSEIEIVN 128
Query: 153 IQNALKKYQKNAKIYLSIRRDGHSIAYPYGYSKTAPSNEAKLKR 196
+ LKK K Y+ I P+G+++T + +L R
Sbjct: 129 VAKYLKKLGDRIKGYMDIHAYSQFWMIPWGHTQTPSKDYQELVR 172
>SB_15763| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 391
Score = 55.2 bits (127), Expect = 6e-08
Identities = 55/210 (26%), Positives = 90/210 (42%), Gaps = 17/210 (8%)
Query: 38 KPVIIIDAGQQGGKESVMLA--LYVIEQLVAC--NDNS--EMIEKVKWVVLPSTNPDGME 91
KPV ++ G +E + A +Y+I Q + +D S E+++K+ ++++P N DG
Sbjct: 137 KPVFFMNCGIHA-REWITPATCMYIITQFITKYRSDKSVTEVLDKMDFIIMPVLNADGYV 195
Query: 92 FARYLMPYWKKNLKPLDD---GPRL--GVDISRNFDKEWNSCPEVENKFSQIYPGAAAAS 146
+ W+K KP G + G D +RN+ +W N S + G A S
Sbjct: 196 YTWTGNRMWRKTRKPNIQSWWGQKTCYGTDPNRNWGYKWGLPGASSNPCSDTFRGKTAQS 255
Query: 147 ENETIFIQNALKKYQKNAKIYLSIRRDGHSIAYPYGYSKTAPSNEAKLKRVASEIATRVN 206
E E + LK K Y+ I P+GY+ + +L RV+ +
Sbjct: 256 EVEVDNVAKFLKGLGPRVKGYMDIHAYSQFWMIPWGYTTANAKDHKELMRVSKIGVDAIR 315
Query: 207 QKTNGIYLFVNTS--IYELEG--KQYC-GH 231
Q Y + ++S IY+ G K Y GH
Sbjct: 316 QTYGTAYRYGSSSNIIYKNSGSSKDYTYGH 345
>SB_62| Best HMM Match : Peptidase_M14 (HMM E-Value=2.7e-34)
Length = 235
Score = 47.6 bits (108), Expect = 1e-05
Identities = 32/141 (22%), Positives = 58/141 (41%), Gaps = 2/141 (1%)
Query: 73 MIEKVKWVVLPSTNPDGMEFARYLMPYWKKNLKPLDDGPRLGVDISRNFDKEWNSCPEVE 132
M++K+ +V++P N DG + W+K KP G G D +RN+ +W
Sbjct: 14 MLDKMDFVIMPILNVDGYVYTHTHNRMWRKTRKPY--GGCYGADPNRNWSFKWGGVGTSS 71
Query: 133 NKFSQIYPGAAAASENETIFIQNALKKYQKNAKIYLSIRRDGHSIAYPYGYSKTAPSNEA 192
+ Y G SE E + + L + +K+ Y+ I +G+++ P
Sbjct: 72 DPCRDTYRGPRPFSEVEALNVARYLYRNRKSLIGYMDIHAYSQLWMTSWGFNRAYPPAYN 131
Query: 193 KLKRVASEIATRVNQKTNGIY 213
+ A + A +K G +
Sbjct: 132 TKVKPAGQKAVEALRKVYGTH 152
>SB_49098| Best HMM Match : Peptidase_M14 (HMM E-Value=4.5e-12)
Length = 290
Score = 37.9 bits (84), Expect = 0.010
Identities = 20/72 (27%), Positives = 33/72 (45%)
Query: 136 SQIYPGAAAASENETIFIQNALKKYQKNAKIYLSIRRDGHSIAYPYGYSKTAPSNEAKLK 195
S IYPG+ SE ET + L K +K + Y+ G P+G++K P N +
Sbjct: 11 SPIYPGSRPFSEVETRNVARYLYKKRKRLRAYVDFHSYGQLWMSPWGFTKKFPPNYRRHA 70
Query: 196 RVASEIATRVNQ 207
R + + + +
Sbjct: 71 RAMNAVVKAIKR 82
>SB_1170| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 561
Score = 33.9 bits (74), Expect = 0.16
Identities = 25/96 (26%), Positives = 44/96 (45%), Gaps = 5/96 (5%)
Query: 31 EKAKEDLKPVIIIDAGQQGGKESVM-LALYVIEQLVACNDNSEMIEKVKWVVLPSTNPDG 89
E + + K + I + G+E ++ L Y+ E + +++ + +LPS NPDG
Sbjct: 111 EPGEPEFKYIANIHGNEVVGRELLLQLIRYLCENYESHEKVRTLVDTTRIHILPSINPDG 170
Query: 90 MEFARYLMPYWKKNLKPLDDGPRLGVDISRNFDKEW 125
E A K K + GVD++RNF ++
Sbjct: 171 YELASVK----GKTHKFIGRRNAYGVDLNRNFPDQF 202
>SB_34368| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 542
Score = 33.1 bits (72), Expect = 0.28
Identities = 22/69 (31%), Positives = 37/69 (53%), Gaps = 5/69 (7%)
Query: 29 EVEKAKEDLKPVIIIDAGQQGGKE-SVMLALYVIEQLVAC--NDN--SEMIEKVKWVVLP 83
+V+ A + KPV G + S +Y I+Q+V D+ + M++K+ +V+LP
Sbjct: 469 QVKGAYKPNKPVFYWQCGIHAREWISPATCMYAIDQMVTKYGKDSGITSMLDKMDFVILP 528
Query: 84 STNPDGMEF 92
NPDG E+
Sbjct: 529 VVNPDGYEY 537
>SB_41552| Best HMM Match : RVT_1 (HMM E-Value=5.4e-32)
Length = 1241
Score = 31.9 bits (69), Expect = 0.65
Identities = 17/49 (34%), Positives = 23/49 (46%), Gaps = 2/49 (4%)
Query: 84 STNPDGMEFARYLMPYWKKNLKPLDDG--PRLGVDISRNFDKEWNSCPE 130
ST+P +E L PYW +PLDD P LG+ + W C +
Sbjct: 933 STSPSSVEACYKLTPYWGNMFRPLDDPDYPWLGIWTTLPIMGVWYWCTD 981
>SB_37941| Best HMM Match : Peptidase_M14 (HMM E-Value=0)
Length = 1328
Score = 31.5 bits (68), Expect = 0.86
Identities = 24/96 (25%), Positives = 43/96 (44%), Gaps = 7/96 (7%)
Query: 31 EKAKEDLKPVIIIDAGQQGGKESVM-LALYVIEQLVACNDNSEMIEKVKWVVLPSTNPDG 89
E + ++K I + GKE ++ L Y+ +++++ + +LPS NPDG
Sbjct: 115 EVKEPEMKYTANIHGNEVVGKEMLLHLIRYLCHNYGVNKRITDLVDSTRIHILPSLNPDG 174
Query: 90 MEFARYLMPYWKKNLKPLDDGPRLGVDISRNFDKEW 125
E A +K GVD++RNF ++
Sbjct: 175 YEMAA------QKTKTHTGTKNANGVDLNRNFPDQF 204
>SB_51769| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 71
Score = 31.1 bits (67), Expect = 1.1
Identities = 17/54 (31%), Positives = 25/54 (46%)
Query: 151 IFIQNALKKYQKNAKIYLSIRRDGHSIAYPYGYSKTAPSNEAKLKRVASEIATR 204
+F + L + ++ A R D S A PY Y +TA AKL+ + A R
Sbjct: 11 LFASDELFRMEELAICRFVTRTDSRSAANPYAYHRTAKLRHAKLRHFYTSSAAR 64
>SB_28263| Best HMM Match : Peptidase_M14 (HMM E-Value=0)
Length = 1258
Score = 30.7 bits (66), Expect = 1.5
Identities = 17/64 (26%), Positives = 33/64 (51%), Gaps = 1/64 (1%)
Query: 31 EKAKEDLKPVIIIDAGQQGGKESVM-LALYVIEQLVACNDNSEMIEKVKWVVLPSTNPDG 89
E K + K + + + GKE ++ A Y+ + ++ ++ ++ + +LPS NPDG
Sbjct: 681 EPGKPEFKYIANMHGNEVVGKEVLLTFAKYLCDNYKKDDEVTKALDTTRVHLLPSMNPDG 740
Query: 90 MEFA 93
E A
Sbjct: 741 YELA 744
>SB_23547| Best HMM Match : NLPC_P60 (HMM E-Value=2.5)
Length = 232
Score = 29.9 bits (64), Expect = 2.6
Identities = 36/164 (21%), Positives = 65/164 (39%), Gaps = 12/164 (7%)
Query: 51 KESVMLALYVIEQLVACNDNSEMIEKVKWVVLPSTNPDGMEFARYLMPYWKKNLKPLD-- 108
+E++ +AL E++ +DN + +E +K + P + F R + + L LD
Sbjct: 34 REAIPIALSW-EEIKTSSDNCDSVENMKEALKTGCTPFSLMFGREM----RTKLPQLDFT 88
Query: 109 -DGPRLGVDISRNFDKEWNSCPEVENKFSQIYPGAAAASENETIFIQNALKKYQKNAKIY 167
P L D R + + + + K S+I G NE Q L+ + K Y
Sbjct: 89 ASDPELARDRDREYKLKLKKHADKKAKESEIKEGDTVLMRNEQ---QGKLEPNFRPEK-Y 144
Query: 168 LSIRRDGHSIAYPYGYSKTAPSNEAKLKRVASEIATRVNQKTNG 211
+ ++G I T N K V + +V+++ G
Sbjct: 145 KVVSKNGSDIVCESETGGTLRRNVQCAKNVPEATSEKVSKENQG 188
>SB_50158| Best HMM Match : Toxin_16 (HMM E-Value=2)
Length = 746
Score = 29.5 bits (63), Expect = 3.5
Identities = 16/51 (31%), Positives = 29/51 (56%), Gaps = 2/51 (3%)
Query: 241 IPLCF-EMRVFLENN-NKIISKFHKMPRGFEASLRNGYFSGIRELYNVIVN 289
+PL + V L+NN N +S+ +++ + FEA+ R Y + N+I+N
Sbjct: 17 LPLLLRDEHVTLQNNRNAALSRLNQLKKRFEAANRKQYREDYIQFMNIIIN 67
Score = 29.5 bits (63), Expect = 3.5
Identities = 16/51 (31%), Positives = 29/51 (56%), Gaps = 2/51 (3%)
Query: 241 IPLCF-EMRVFLENN-NKIISKFHKMPRGFEASLRNGYFSGIRELYNVIVN 289
+PL + V L+NN N +S+ +++ + FEA+ R Y + N+I+N
Sbjct: 98 LPLLLRDEHVTLQNNRNAALSRLNQLKKRFEAANRKQYREDYIQFMNIIIN 148
>SB_15157| Best HMM Match : DUF1153 (HMM E-Value=1.7)
Length = 412
Score = 29.5 bits (63), Expect = 3.5
Identities = 33/153 (21%), Positives = 59/153 (38%), Gaps = 11/153 (7%)
Query: 62 EQLVACNDNSEMIEKVKWVVLPSTNPDGMEFARYLMPYWKKNLKPLD---DGPRLGVDIS 118
E++ +DN + +E +K + P + F R + + L LD P L D
Sbjct: 224 EEIKTSSDNCDSVENMKEALKTGCTPFSLMFGREM----RTKLPQLDFTASDPELARDRD 279
Query: 119 RNFDKEWNSCPEVENKFSQIYPGAAAASENETIFIQNALKKYQKNAKIYLSIRRDGHSIA 178
R + + + + K S+I G NE Q L+ + K Y + ++G I
Sbjct: 280 REYKLKLKKHADKKAKESEIKEGDTVLMRNEQ---QGKLEPNFRPEK-YKVVSKNGSDIV 335
Query: 179 YPYGYSKTAPSNEAKLKRVASEIATRVNQKTNG 211
T N K+V + +V+++ G
Sbjct: 336 CESETGGTLRRNVQCAKKVPEATSEKVSKENQG 368
>SB_27034| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 629
Score = 29.1 bits (62), Expect = 4.6
Identities = 19/76 (25%), Positives = 34/76 (44%), Gaps = 1/76 (1%)
Query: 126 NSCPEVENKFSQIYPGAAAASENETIFIQNALKKYQKNAKIYLSIRRDGHSIAYPYGYSK 185
NS E + F A +E T +++ +K+ K+ + + + R + PY Y K
Sbjct: 344 NSLLEEDLNFDHTAVAAPPITEETTQNLEDIIKQRIKD-EAWDDVERKAKPVLQPYEYKK 402
Query: 186 TAPSNEAKLKRVASEI 201
P ++ K K S+I
Sbjct: 403 KMPLDQEKSKLSLSQI 418
>SB_18998| Best HMM Match : Exo_endo_phos (HMM E-Value=1.1e-10)
Length = 422
Score = 28.7 bits (61), Expect = 6.1
Identities = 13/34 (38%), Positives = 20/34 (58%), Gaps = 4/34 (11%)
Query: 104 LKPLDDGPRLGVDISRNFDKEWNSCPEVENKFSQ 137
LKP++ P LGV++S D W+ + NK S+
Sbjct: 341 LKPVEHNPYLGVELSNTLDWSWH----INNKVSK 370
>SB_13109| Best HMM Match : Dynein_heavy (HMM E-Value=2.6e-09)
Length = 1703
Score = 28.7 bits (61), Expect = 6.1
Identities = 16/59 (27%), Positives = 30/59 (50%)
Query: 4 QLLDGDEASCIEDASIGSSAVVINKEVEKAKEDLKPVIIIDAGQQGGKESVMLALYVIE 62
+L DGD +E++ + V++ E+ L+P+++ +QGG + L VIE
Sbjct: 1178 KLTDGDYIRTLENSIQFGNPVLLENVGEELDPSLEPLLLKQTFKQGGVVCIRLGESVIE 1236
>SB_59792| Best HMM Match : RVT_1 (HMM E-Value=4.2039e-45)
Length = 565
Score = 28.3 bits (60), Expect = 8.0
Identities = 17/67 (25%), Positives = 28/67 (41%), Gaps = 3/67 (4%)
Query: 179 YPYGYSKTAPSNEAKLKRVASEIATRVNQKTNGIYLFVNTSIYELEGKQYCG---HSVDY 235
Y + Y PS + K + ++I + NQ I T+ + + K YC ++Y
Sbjct: 360 YHHSYLGVLPSGDLKWDKHIAQITSSANQTLGVIRRNFRTASVDCKSKLYCSLVRPKLEY 419
Query: 236 ANDLGIP 242
AN P
Sbjct: 420 ANSAWYP 426
>SB_24812| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 1384
Score = 28.3 bits (60), Expect = 8.0
Identities = 14/45 (31%), Positives = 23/45 (51%)
Query: 248 RVFLENNNKIISKFHKMPRGFEASLRNGYFSGIRELYNVIVNQKK 292
RV L++ NK +F KM ++G +RE + I+N+ K
Sbjct: 415 RVELDDRNKSTLEFKKMEEDLRVKGKDGLSGQLREEFEKILNEHK 459
>SB_53466| Best HMM Match : SCP (HMM E-Value=2.4e-27)
Length = 5087
Score = 28.3 bits (60), Expect = 8.0
Identities = 18/62 (29%), Positives = 31/62 (50%)
Query: 15 EDASIGSSAVVINKEVEKAKEDLKPVIIIDAGQQGGKESVMLALYVIEQLVACNDNSEMI 74
E+AS + I KE E+ E + V+ +AG++ +E V + + + + SE I
Sbjct: 1622 EEASALGISEEIEKETEETVEQHEEVVESEAGEKVEEEEVPAVVVALSEEASALGISEEI 1681
Query: 75 EK 76
EK
Sbjct: 1682 EK 1683
>SB_6367| Best HMM Match : Seryl_tRNA_N (HMM E-Value=0.84)
Length = 563
Score = 28.3 bits (60), Expect = 8.0
Identities = 24/96 (25%), Positives = 41/96 (42%), Gaps = 2/96 (2%)
Query: 130 EVENKFSQIYPGAAAASENETIFIQNALKKYQKNAKIYLSIRRDGHSIAYPYGYSKTAPS 189
+VE+ F+ + A + ET + YQ++ R G A+ G SK+A S
Sbjct: 116 QVESMFNALLDMRKGARKMETACAETQTVIYQEDGSTQWEERDIGPMRAH--GTSKSASS 173
Query: 190 NEAKLKRVASEIATRVNQKTNGIYLFVNTSIYELEG 225
N+A+ + ++ ++K VNT L G
Sbjct: 174 NKARDVAMVDDVVGLTSEKNGESVTEVNTDKKVLTG 209
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.316 0.135 0.392
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,258,607
Number of Sequences: 59808
Number of extensions: 430919
Number of successful extensions: 915
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 896
Number of HSP's gapped (non-prelim): 24
length of query: 298
length of database: 16,821,457
effective HSP length: 82
effective length of query: 216
effective length of database: 11,917,201
effective search space: 2574115416
effective search space used: 2574115416
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 60 (28.3 bits)
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