Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001886-TA|BGIBMGA001886-PA|IPR007259|Spc97/Spc98
(486 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3 s... 61 4e-10
SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit Alp4|... 55 3e-08
SPBC211.06 |gfh1||gamma tubulin complex subunit Gfh1|Schizosacch... 38 0.003
SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr 2|||... 29 1.5
SPBC3F6.05 |rga1||GTPase activating protein Rga1|Schizosaccharom... 29 1.5
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 28 2.5
SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|c... 28 3.4
SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces... 27 4.4
SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces ... 27 5.9
SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual 27 7.8
SPBC336.08 |spc24||spindle pole body protein Spc24|Schizosacchar... 27 7.8
>SPBC428.20c |alp6|SPBC902.01c|gamma tubulin complex Spc98/GCP3
subunit Alp6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 821
Score = 60.9 bits (141), Expect = 4e-10
Identities = 99/455 (21%), Positives = 172/455 (37%), Gaps = 60/455 (13%)
Query: 4 QILSLIHQYILSGNKSIHDAVLKIFTSVNKVFIHQLCTWLLYGELKDVYQEFFISKRKDD 63
+++ ++ +Y + G+ I + KI T + + W+ GEL D YQEFF+ K+
Sbjct: 344 RLIQVVSKYNVHGDPLIQELSDKILTEITGPLYEMIENWIYKGELVDPYQEFFV---KEK 400
Query: 64 XXXXXXXXXXXXNVCDKTLVSTETLGCGYTLNPTLIPHFMPLSLAQEILFIGKTVILFDL 123
+V K Y L+ LIP F+ L +I IGK++
Sbjct: 401 NGSESHDHQGQGDVVWKG---------KYFLDKELIPSFLSEELVDKIFLIGKSL----- 446
Query: 124 DPKKVKKHSGFASSRPMLPLKKYGIESRRFTIWEDKESEFHEKLQKLKDPDNFEVHNLRS 183
FA +YG F W + + K +DP H+L +
Sbjct: 447 ---------NFA---------RYGCGD--FD-WAQEHYQKLVKKLSYRDP-----HSLET 480
Query: 184 VIREIKECVTKHLWTVAVEEAQLIHELKLMKDFYXXXXXXXXXXXXXXTAHMLDKPTIRT 243
V+ + HL + E L LK +K + + LD+P
Sbjct: 481 VVDKAYTESINHLVYLMEEVFHLTDHLKAIKKYLLLGQGDFVDLLMESLGNSLDQPANTL 540
Query: 244 SSRDMNQAFQMAARSVFLSNNTDIEKFSFELPYVKPNISGNSSIDEDYSTDGWSSIILKY 303
++ + + A RS SN S+E YV + ++ + GW L+Y
Sbjct: 541 FRHNLTASLESAIRS---SN------ASYEPEYVLKRLDAR-LLELSHGETGWDVFTLEY 590
Query: 304 DFKWPLHLLFTPEVLARYNDMFRLLLRLKKTQHDLHSLWKTYKQSASFAMSQL-HNKL-- 360
P++++ TP +Y +F L RLK+ + L W+ L + K
Sbjct: 591 KVDSPINVIITPYCSRQYLKIFNFLWRLKRIEFALAHSWRRVNLGERNVFRNLDYTKFEW 650
Query: 361 MFLMDNLQHYMQADVLETNFSRLMDAVNKTN-DFEKLKKAHATFLADILSQSFLTVTLSS 419
F+ +L + V+E ++ L A+ K N + +AH ++ I + L S
Sbjct: 651 HFVSCHLAEMIHF-VIEISWQELQLAMEKPNATLDTYIEAHEKYVTSITHKGLLGGGKSR 709
Query: 420 DSDCSGDPADTLNNPVFCNIMELLKLCHSF-CSMS 453
+ D + V N + ++L ++F CS+S
Sbjct: 710 NEDSFLHQLHDILK-VILNFHDAIELLYNFSCSLS 743
>SPBC365.15 |alp4||gamma tubulin complex Spc97/GCP2 subunit
Alp4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 784
Score = 54.8 bits (126), Expect = 3e-08
Identities = 30/146 (20%), Positives = 68/146 (46%), Gaps = 14/146 (9%)
Query: 283 GNSSIDEDYSTDGWSSIILKYDFKWPLHLLFTPEVLARYNDMFRLLLRLKKTQHDLHSLW 342
G S + D +G+ ++ L Y +PL L+ + + + RY +FR L L+ + L + W
Sbjct: 550 GTSKVGSDKDINGFETMQLCYKVPFPLSLILSRKAIIRYQLLFRYFLLLRHVEMQLENSW 609
Query: 343 KTYKQSASFAMSQ--------------LHNKLMFLMDNLQHYMQADVLETNFSRLMDAVN 388
+ +++++ ++ L +++ + + +Y ++V+ET++ + M +
Sbjct: 610 VQHSKNSAWRLNSSNAKIEQWKRNSWLLRTRMLSFVQKIIYYTTSEVIETHWGKFMGELE 669
Query: 389 KTNDFEKLKKAHATFLADILSQSFLT 414
+ L + H FL L + LT
Sbjct: 670 NARTVDNLMQEHIDFLDTCLKECMLT 695
>SPBC211.06 |gfh1||gamma tubulin complex subunit
Gfh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 577
Score = 37.9 bits (84), Expect = 0.003
Identities = 23/95 (24%), Positives = 50/95 (52%), Gaps = 3/95 (3%)
Query: 306 KWPLHLLFTPEVLARYNDMFRLLLRLKKTQHDLHSLWKTYKQSASFAMSQLHNKL---MF 362
KWP +L L Y+D++ LL L + + SL+ K+ + +SQ +L ++
Sbjct: 397 KWPFNLFMDKNALNVYSDLWILLGSLHLSITKIKSLFYERKEYTAKGISQPWTQLWVTLW 456
Query: 363 LMDNLQHYMQADVLETNFSRLMDAVNKTNDFEKLK 397
+ +LQ+Y V++ ++ +L +++ + +KL+
Sbjct: 457 FLSSLQYYAYECVIKPSYCKLRESLTELYRTQKLR 491
>SPBC577.09 |||ERCC-8 homolog |Schizosaccharomyces pombe|chr
2|||Manual
Length = 404
Score = 29.1 bits (62), Expect = 1.5
Identities = 15/31 (48%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
Query: 341 LWKTYKQSASFAMSQLHNK-LMFLMDNLQHY 370
LW K S+SFA LHNK L N+ HY
Sbjct: 216 LWDIRKVSSSFACMDLHNKYLPSNQTNISHY 246
>SPBC3F6.05 |rga1||GTPase activating protein
Rga1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1150
Score = 29.1 bits (62), Expect = 1.5
Identities = 28/118 (23%), Positives = 52/118 (44%), Gaps = 2/118 (1%)
Query: 282 SGNSSIDEDYSTDGWSSIILKYDFKWPLHLLFTPEVLARYNDMF-RLLLRLKKTQHDLHS 340
S N S DE + + + S + +Y + + L+ +L ND R L + Q ++ S
Sbjct: 543 SSNVSNDEPHPSFEYVSRLSQYIYLLRIALVRLYTILMENNDSSQRKPLSVDPKQENVSS 602
Query: 341 LWKTYKQSASFAMSQLHNKLMFLMDNLQHYMQADVLETNFSRLMDAVNKTNDFEKLKK 398
+T KQ+ + A S K +++L+H + + + + N T + KL K
Sbjct: 603 TVETAKQAETTASSDSFRKYANTLNDLRHLKSSRNRKATSNETQSSSNST-ETSKLSK 659
>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 4196
Score = 28.3 bits (60), Expect = 2.5
Identities = 19/66 (28%), Positives = 34/66 (51%), Gaps = 6/66 (9%)
Query: 330 RLKKTQHDLHSLWKTYKQSASFAMSQLHNK-LMFLMDNLQ-----HYMQADVLETNFSRL 383
++ KT + + W+ Y +S S QLH++ ++F++D L H + + ETN
Sbjct: 274 QISKTALEEFNFWQFYYRSLSRLNDQLHSRPVLFVLDILAFGKRFHTIASFNSETNIQCF 333
Query: 384 MDAVNK 389
+D V K
Sbjct: 334 VDKVGK 339
>SPAC2G11.09 |||DUF221 family protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 796
Score = 27.9 bits (59), Expect = 3.4
Identities = 22/98 (22%), Positives = 43/98 (43%), Gaps = 3/98 (3%)
Query: 325 FRLLLRLKKTQHDLHSLWKTYKQSASFAMSQLHNKLMFLMDNLQHYMQADVLETNFSRLM 384
+ L+ +++ QH LW T F + +M LM + Y + V+ F L
Sbjct: 672 YELIYQMEHPQHSTGELWSTIFLRMIFGCVIMQLTMMGLMSLRKAYWLSTVI---FPLLC 728
Query: 385 DAVNKTNDFEKLKKAHATFLADILSQSFLTVTLSSDSD 422
V +F + ++ F++ ++ + TLSS+S+
Sbjct: 729 FTVISAYNFSTMIRSSMQFVSLYYIRTHQSNTLSSESE 766
>SPCC645.05c |myo2|rng5|myosin II heavy chain|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 1526
Score = 27.5 bits (58), Expect = 4.4
Identities = 18/55 (32%), Positives = 28/55 (50%), Gaps = 3/55 (5%)
Query: 164 HEKLQKLKDPDNFEVHNLRSVIREIKEC-VTKHLWTVAVEEAQLIH--ELKLMKD 215
+E L++ D N E HNL + E+K V+ +++ L H ELK +KD
Sbjct: 1414 YENLKRDYDRLNLEKHNLEKQVNELKGAEVSPQPTGQSLQHVNLAHAIELKALKD 1468
>SPAC664.10 |klp2||kinesin-like protein Klp2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 817
Score = 27.1 bits (57), Expect = 5.9
Identities = 16/51 (31%), Positives = 25/51 (49%)
Query: 329 LRLKKTQHDLHSLWKTYKQSASFAMSQLHNKLMFLMDNLQHYMQADVLETN 379
L++ + Q + SL K ++ AS AM ++ NK LQ +Q E N
Sbjct: 320 LKISRLQEEKDSLLKKVQEGASLAMQRVQNKHDLEKKRLQSAIQPLQEENN 370
>SPBP8B7.09c |||karyopherin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 978
Score = 26.6 bits (56), Expect = 7.8
Identities = 20/78 (25%), Positives = 39/78 (50%), Gaps = 3/78 (3%)
Query: 353 MSQLHNKLMFLMDNLQHYMQADVLETNFSRLMDAVNKTNDFEK---LKKAHATFLADILS 409
+SQL + M + + M +L FS L A T+D K L+K++ +F+ +L+
Sbjct: 761 ISQLIHIYKDNMMEITNRMLPTLLMRIFSSLSAAPQGTDDAVKQNDLRKSYISFILQLLN 820
Query: 410 QSFLTVTLSSDSDCSGDP 427
+ F ++ + ++ DP
Sbjct: 821 KGFGSILFTEENQVYFDP 838
>SPBC336.08 |spc24||spindle pole body protein
Spc24|Schizosaccharomyces pombe|chr 2|||Manual
Length = 198
Score = 26.6 bits (56), Expect = 7.8
Identities = 18/65 (27%), Positives = 33/65 (50%), Gaps = 1/65 (1%)
Query: 316 EVLARYNDMFRLLLRLKKTQHDLHSLWKTYKQSASFAMSQLHNKLMFLMDNLQHYMQADV 375
E+L+R + FR+ L T++++ SL ++ Q + QL + D Q A++
Sbjct: 79 EILSREKEKFRIAKLLNITENEIMSL-ESQLQKMKEQLLQLEERENTSEDICQSEENANM 137
Query: 376 LETNF 380
L+ NF
Sbjct: 138 LKLNF 142
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.321 0.135 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,187,353
Number of Sequences: 5004
Number of extensions: 90340
Number of successful extensions: 297
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 286
Number of HSP's gapped (non-prelim): 17
length of query: 486
length of database: 2,362,478
effective HSP length: 75
effective length of query: 411
effective length of database: 1,987,178
effective search space: 816730158
effective search space used: 816730158
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 56 (26.6 bits)
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