Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001886-TA|BGIBMGA001886-PA|IPR007259|Spc97/Spc98
(486 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g53760.1 68416.m05939 tubulin family protein similar to SP|Q9... 146 4e-35
At5g06680.1 68418.m00754 tubulin family protein similar to SP|Q9... 64 2e-10
At5g17410.2 68418.m02043 tubulin family protein similar to spind... 60 4e-09
At5g17410.1 68418.m02042 tubulin family protein similar to spind... 60 4e-09
At1g20570.1 68414.m02565 tubulin family protein 54 3e-07
At1g80260.1 68414.m09396 tubulin family protein 48 1e-05
At3g43610.1 68416.m04640 tubulin family protein 41 0.002
At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NA... 31 1.3
At3g63200.1 68416.m07100 patatin-related low similarity to patat... 31 2.3
At3g28040.1 68416.m03500 leucine-rich repeat transmembrane prote... 30 4.0
At5g24390.1 68418.m02875 RabGAP/TBC domain-containing protein si... 29 5.2
At4g02310.1 68417.m00312 kelch repeat-containing F-box family pr... 29 6.9
At3g44160.1 68416.m04734 chloroplast outer membrane protein-rela... 29 9.1
At3g23430.1 68416.m02953 phosphate transporter, putative (PHO1) ... 29 9.1
At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NA... 29 9.1
>At3g53760.1 68416.m05939 tubulin family protein similar to
SP|Q9SC88 Gamma-tubulin complex component 4 homolog
{Medicago truncatula}, SP|Q9UGJ1|GCP4_HUMAN
Gamma-tubulin complex component 4 {Homo sapiens};
contains Pfam profile PF04130: Spc97 / Spc98 family
Length = 745
Score = 146 bits (353), Expect = 4e-35
Identities = 114/475 (24%), Positives = 209/475 (44%), Gaps = 65/475 (13%)
Query: 2 GCQILSLIHQYILSGNKSIHDAVLKIFTSVNKVFIHQLCTWLLYGELKDVYQEFFISKRK 61
G Q+L+++++ G + + ++ + ++V +QL W++YG L+D + EFFI ++
Sbjct: 178 GGQLLNVLNKRCHCGVPELRTCLQRLLWNGHQVMYNQLAAWMVYGILQDPHGEFFIKRQD 237
Query: 62 DDXXXXXXXXXXXXNVCDKTLVSTETLG---CGYTLNPTLIPHFMPLSLAQEILFIGKTV 118
D +T V +L G+ ++ ++P ++P+ L + ILF GK +
Sbjct: 238 DGDLDHRSSQEEVSEKLARTSVHETSLTDWHSGFHISLDMLPDYIPMRLGESILFAGKAI 297
Query: 119 -ILFDLDP----KKVKK---------------HSGFASSRPMLPLKKYGIESRRFTIWED 158
+L + P +K K HS F + L G E + +
Sbjct: 298 RVLRNPSPAFQFQKDKSFQQTMRGSQRIRGFMHSDFPETETELDADLTGGE----LLPQS 353
Query: 159 KESEFHEKLQKLKDPDNFEVHNLRSVIREIKECVTKHLWTVAVEEAQLIHELKLMKDFYX 218
+ + L+ LK+ F + + ++ HLW + V A L LK +KD++
Sbjct: 354 EADKIEAMLKDLKESSEFHKRSFECTVDSVRAIAASHLWQLVVVRADLNGHLKALKDYFL 413
Query: 219 XXXXXXXXXXXXXTAHMLDKPTIR-TSSRDMNQAFQMAARSVFLSNNTDIEKFSFELPYV 277
+ ++ P + T D+ FQ+AA + + S +P
Sbjct: 414 LEKGDFFQCFLEESRQLMRLPPRQSTGESDLMVPFQLAATKTIAEEDKYFSRVSLRMPSF 473
Query: 278 --------------KPNISGNSSIDEDYSTDGWSSIILKYDFKWPLHLLFTPEVLARYND 323
K +++G +++ D S DGW +I L+Y WP+ L FT EVL++Y
Sbjct: 474 GVTVRSSQADMVRSKVSLTGKANLTSDTSVDGWDAIALEYSVDWPMQLFFTQEVLSKYLK 533
Query: 324 MFRLLLRLKKTQHDLHSLW-----KTYKQSASF------------------AMSQLHNKL 360
+F+ L+RLK+TQ +L W + + +SA M ++ +
Sbjct: 534 VFQYLIRLKRTQMELEKSWASVMHQDHIESAQHRKDGLNGSTSQQRRQGIRPMWRVREHM 593
Query: 361 MFLMDNLQHYMQADVLETNFSRLMDAVNKTNDFEKLKKAHATFLADILSQSFLTV 415
FL+ NLQ Y+Q DV+E+ + L ++ + DF +L H +L+ ++SQSFL +
Sbjct: 594 AFLIRNLQFYIQVDVIESQWKVLQTHIHDSQDFTELVGFHQEYLSALISQSFLDI 648
>At5g06680.1 68418.m00754 tubulin family protein similar to
SP|Q96CW5 Gamma-tubulin complex component 3 {Homo
sapiens}
Length = 838
Score = 64.5 bits (150), Expect = 2e-10
Identities = 42/139 (30%), Positives = 66/139 (47%), Gaps = 20/139 (14%)
Query: 295 GWSSIILKYDFKWPLHLLFTPEVLARYNDMFRLLLRLKKTQHDLHSLWKTYK----QSAS 350
GW L+Y+ + PL +FT VL++Y +F L +LK+ +H L +WKT K S S
Sbjct: 576 GWDVFSLEYEARVPLDTVFTESVLSKYLRVFNFLWKLKRVEHALIGIWKTMKPNCITSNS 635
Query: 351 FAMSQ----------------LHNKLMFLMDNLQHYMQADVLETNFSRLMDAVNKTNDFE 394
F Q L N++ + N Q+Y+ +VLE ++S + D +
Sbjct: 636 FVKLQSSVKLQLLSALRRCQVLWNEMNHFVTNFQYYIMFEVLEVSWSNFSKEMEAAKDLD 695
Query: 395 KLKKAHATFLADILSQSFL 413
L AH +L I+ +S L
Sbjct: 696 DLLAAHEKYLNAIVGKSLL 714
Score = 43.6 bits (98), Expect = 3e-04
Identities = 32/117 (27%), Positives = 52/117 (44%), Gaps = 20/117 (17%)
Query: 2 GCQILSLIHQYILSGNKSIHDAVLKIFTSVNKVFIHQLCTWLLYGELKDVYQEFFISKRK 61
G + IH + G+ +HD ++ + V + +W+L GEL+D + EFF+
Sbjct: 336 GGAMAGAIHLHAQHGDPLVHDFMMSLLRCVCSPLFEMVRSWVLEGELEDTFGEFFV---- 391
Query: 62 DDXXXXXXXXXXXXNVCDKTLVSTETLGCGYTLNPTLIPHFMPLSLAQEILFIGKTV 118
L+ E GY L+P ++P F+ SLAQ IL GK++
Sbjct: 392 ------------VGQPVKVDLLWRE----GYKLHPAMLPSFISPSLAQRILRTGKSI 432
>At5g17410.2 68418.m02043 tubulin family protein similar to spindle
pole body protein [Homo
sapiens][GI:2801701][PMID:9566967], gamma-tubulin ring
protein Dgrip84 [Drosophila
melanogaster][GI:4689225][PMID: 10037793]
Length = 679
Score = 59.7 bits (138), Expect = 4e-09
Identities = 33/129 (25%), Positives = 63/129 (48%), Gaps = 8/129 (6%)
Query: 284 NSSIDEDYSTDGWSSIILKYDFKWPLHLLFTPEVLARYNDMFRLLLRLKKTQHDLHSLWK 343
++SI++ S G + L Y +WPL ++ + + L++Y +FR L K + L W+
Sbjct: 448 SNSIEDPMSITGLETFSLSYKVQWPLSIVISKKALSKYQLIFRFLFHCKHVERQLCGAWQ 507
Query: 344 TY--------KQSASFAMSQLHNKLMFLMDNLQHYMQADVLETNFSRLMDAVNKTNDFEK 395
+ K +A S L ++ + +L HY+ +VLE N+ + D + T ++
Sbjct: 508 IHQGIRSMNSKGTAILRSSLLCRSMLKFISSLLHYLTFEVLEPNWHVMHDRLQSTRSVDE 567
Query: 396 LKKAHATFL 404
+ + H FL
Sbjct: 568 VIQHHDFFL 576
Score = 31.1 bits (67), Expect = 1.7
Identities = 15/63 (23%), Positives = 33/63 (52%), Gaps = 2/63 (3%)
Query: 1 MGCQILSLIHQYI--LSGNKSIHDAVLKIFTSVNKVFIHQLCTWLLYGELKDVYQEFFIS 58
+G +L+L+ ++G+ S+ + K+ + ++ L W+ G + D Y EFFI+
Sbjct: 203 VGSGVLNLLQSQAKAMAGDNSVRSLLEKMTECASNAYLSILERWVYEGIIDDPYGEFFIA 262
Query: 59 KRK 61
+ +
Sbjct: 263 ENR 265
>At5g17410.1 68418.m02042 tubulin family protein similar to spindle
pole body protein [Homo
sapiens][GI:2801701][PMID:9566967], gamma-tubulin ring
protein Dgrip84 [Drosophila
melanogaster][GI:4689225][PMID: 10037793]
Length = 678
Score = 59.7 bits (138), Expect = 4e-09
Identities = 33/129 (25%), Positives = 63/129 (48%), Gaps = 8/129 (6%)
Query: 284 NSSIDEDYSTDGWSSIILKYDFKWPLHLLFTPEVLARYNDMFRLLLRLKKTQHDLHSLWK 343
++SI++ S G + L Y +WPL ++ + + L++Y +FR L K + L W+
Sbjct: 447 SNSIEDPMSITGLETFSLSYKVQWPLSIVISKKALSKYQLIFRFLFHCKHVERQLCGAWQ 506
Query: 344 TY--------KQSASFAMSQLHNKLMFLMDNLQHYMQADVLETNFSRLMDAVNKTNDFEK 395
+ K +A S L ++ + +L HY+ +VLE N+ + D + T ++
Sbjct: 507 IHQGIRSMNSKGTAILRSSLLCRSMLKFISSLLHYLTFEVLEPNWHVMHDRLQSTRSVDE 566
Query: 396 LKKAHATFL 404
+ + H FL
Sbjct: 567 VIQHHDFFL 575
Score = 31.1 bits (67), Expect = 1.7
Identities = 15/63 (23%), Positives = 33/63 (52%), Gaps = 2/63 (3%)
Query: 1 MGCQILSLIHQYI--LSGNKSIHDAVLKIFTSVNKVFIHQLCTWLLYGELKDVYQEFFIS 58
+G +L+L+ ++G+ S+ + K+ + ++ L W+ G + D Y EFFI+
Sbjct: 202 VGSGVLNLLQSQAKAMAGDNSVRSLLEKMTECASNAYLSILERWVYEGIIDDPYGEFFIA 261
Query: 59 KRK 61
+ +
Sbjct: 262 ENR 264
>At1g20570.1 68414.m02565 tubulin family protein
Length = 976
Score = 53.6 bits (123), Expect = 3e-07
Identities = 55/246 (22%), Positives = 98/246 (39%), Gaps = 21/246 (8%)
Query: 185 IREIKECVTKHLWTVAVEEAQLIHELKLMKDFYXXXXXXXXXXXXXXTAHMLDKPTIRTS 244
IR + ++K +++ + E +L+HEL +++ Y L K
Sbjct: 632 IRRQVDNISKVIFSKLMNEWKLMHELAVLRAIYLLGSGDLLQHFLTVIFDRLGKGESSND 691
Query: 245 SRDMNQAFQMAARS--------------VFLSNNTDIEKFSFELPYVKPNISGNSSIDED 290
++N Q + R+ V +S+ +++ + VK S S +
Sbjct: 692 DFELNIIIQESIRNSADTMLLSSPDALVVSISSEGCLDRDKDDKGDVKSLSSPRESSVNN 751
Query: 291 YSTDGWSSIILKYDFKWPLHLLFTPEVLARYNDMFRLLLRLKKTQHDLHSLWKTYKQSAS 350
Y+ D S+ Y WPL L+ E + +YN + R L K + +WK K SA+
Sbjct: 752 YAIDCLESLKFTYKVPWPLELIANSEAIKKYNQVKRAKYVLDKAR---RLMWKG-KGSAT 807
Query: 351 FAMSQ---LHNKLMFLMDNLQHYMQADVLETNFSRLMDAVNKTNDFEKLKKAHATFLADI 407
L KL+ +D Y+ V T + L +A+ K +++ H T+L I
Sbjct: 808 KIRKHHCLLEQKLLNFVDAFHQYVMDRVYHTAWRELCEAMVKAGSLDEVIDVHETYLLSI 867
Query: 408 LSQSFL 413
Q F+
Sbjct: 868 QRQCFV 873
>At1g80260.1 68414.m09396 tubulin family protein
Length = 951
Score = 48.0 bits (109), Expect = 1e-05
Identities = 37/138 (26%), Positives = 57/138 (41%), Gaps = 7/138 (5%)
Query: 279 PNISGNSSIDEDYSTDGWSSIILKYDFKWPLHLLFTPEVLARYNDMFRLLLRLKKTQHDL 338
P S S + D S+ Y WPL L+ E + +YN + R L K +
Sbjct: 701 PLSSTRKSRVNSFGIDCLESLKFTYKVPWPLELIANSEAIKKYNQVKRAKYVLDKAR--- 757
Query: 339 HSLWKTYKQSASFAMSQ---LHNKLMFLMDNLQHYMQADVLETNFSRLMDAVNKTNDFEK 395
+WK K SA+ L KL+ +D Y+ V T + L +A+ K ++
Sbjct: 758 RWMWKG-KGSATKIRKHHWLLEQKLLNFVDAFHQYVMDRVYHTAWRELCEAMVKAGSLDE 816
Query: 396 LKKAHATFLADILSQSFL 413
+ H T+L I Q F+
Sbjct: 817 VIYVHETYLLSIQRQCFV 834
>At3g43610.1 68416.m04640 tubulin family protein
Length = 1120
Score = 40.7 bits (91), Expect = 0.002
Identities = 26/107 (24%), Positives = 55/107 (51%), Gaps = 6/107 (5%)
Query: 301 LKYDFKWPLHLLFTPEVLARYNDMFRLLLRLKKTQHDLHSLWKTYKQSASFAMSQLHNKL 360
L Y WP+ ++ T + L Y D+F L+++K + L +W + K + +H +
Sbjct: 980 LGYRVDWPISIILTCDALTAYADVFSFLVQVKLAAYVLTDVWCSLKVKDMMDLESVH--M 1037
Query: 361 MFLMDNLQHYMQAD--VLETNFSRLMDAVNKTNDFEK-LKKAHATFL 404
+L + L+ +Q+ V ++++ + +N + +F+K LK+ H L
Sbjct: 1038 AYLSEALRIGIQSTDRVPNDSWTKTL-GINTSQNFDKELKELHKCHL 1083
>At5g14590.1 68418.m01711 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase (NADP+) [Nicotiana tabacum]
GI:3021512; contains Pfam domain PF00180: dehydrogenase,
isocitrate/isopropylmalate family
Length = 485
Score = 31.5 bits (68), Expect = 1.3
Identities = 31/129 (24%), Positives = 59/129 (45%), Gaps = 13/129 (10%)
Query: 286 SIDEDYSTDGWSSIILKYDFKWPLHLLFTPEVLARYN----DMFRLLLRLK-KTQHDLHS 340
++DE SS+ + KWPL+L +L +Y+ D+F+ + K + + HS
Sbjct: 255 NVDESIRAFAESSMAMALTKKWPLYLSTKNTILKKYDGRFKDIFQEVYEANWKQKFEEHS 314
Query: 341 LWKTYK---QSASFAMSQLHNKLMFLMDNLQHYMQADVLETNFSR--LMDAVNKTNDFEK 395
+W ++ ++A+ ++ N +Q+D+L F LM +V + D +
Sbjct: 315 IWYEHRLIDDMVAYAVKS-EGGYVWACKNYDGDVQSDLLAQGFGSLGLMTSVLLSADGKT 373
Query: 396 LKK--AHAT 402
L+ AH T
Sbjct: 374 LESEAAHGT 382
>At3g63200.1 68416.m07100 patatin-related low similarity to
patatin-like latex protein allergen Hev b 7 - Hevea
brasiliensis, EMBL:AF113546; contains patatin domain
PF01734
Length = 384
Score = 30.7 bits (66), Expect = 2.3
Identities = 21/77 (27%), Positives = 34/77 (44%), Gaps = 1/77 (1%)
Query: 380 FSRLMDAVNKTNDFEKLKKAHATFLADILSQSFLTVTLSSDSDCSG-DPADTLNNPVFCN 438
FSR + + + DFE K AT L + F V++ + CS D +NNP
Sbjct: 174 FSRAGASESPSFDFELWKVCRATSATPSLFKPFSVVSVDGKTSCSAVDGGLVMNNPTAAA 233
Query: 439 IMELLKLCHSFCSMSEI 455
+ +L F S++ +
Sbjct: 234 VTHVLHNKRDFPSVNGV 250
>At3g28040.1 68416.m03500 leucine-rich repeat transmembrane protein
kinase, putative contains Pfam profiles: PF00560 leucine
rich repeat, PF00069 eukaryotic protein kinase domain
Length = 1016
Score = 29.9 bits (64), Expect = 4.0
Identities = 22/88 (25%), Positives = 42/88 (47%), Gaps = 4/88 (4%)
Query: 364 MDNLQHYMQADVLETNFSRLMDAVNKTNDFEKLKKAHATFLADILSQSFLTVTLSSDSDC 423
+ LQ + NF+ ++A++ N +KL +H I S S ++T D
Sbjct: 97 IQKLQRLKVLSLSNNNFTGNINALSNNNHLQKLDLSHNNLSGQIPS-SLGSITSLQHLDL 155
Query: 424 SGDP-ADTLNNPVF--CNIMELLKLCHS 448
+G+ + TL++ +F C+ + L L H+
Sbjct: 156 TGNSFSGTLSDDLFNNCSSLRYLSLSHN 183
>At5g24390.1 68418.m02875 RabGAP/TBC domain-containing protein
similar to GTPase activating protein [Yarrowia
lipolytica] GI:2370595; contains Pfam profile PF00566:
TBC domain
Length = 528
Score = 29.5 bits (63), Expect = 5.2
Identities = 17/47 (36%), Positives = 28/47 (59%), Gaps = 2/47 (4%)
Query: 155 IWEDKESEFHEKLQKLKDPDNFEVHNLRSVI--REIKECVTKHLWTV 199
I + K+S+ ++ L+K+K D F V+ + V+ RE+ T HLW V
Sbjct: 388 IIKSKDSQLYKHLEKVKAEDCFFVYRMVLVMFRRELTLEQTLHLWEV 434
>At4g02310.1 68417.m00312 kelch repeat-containing F-box family
protein contains Pfam PF00646: F-box domain; contains
Pfam PF01344 : Kelch motif;
Length = 349
Score = 29.1 bits (62), Expect = 6.9
Identities = 13/34 (38%), Positives = 20/34 (58%)
Query: 100 PHFMPLSLAQEILFIGKTVILFDLDPKKVKKHSG 133
P+F L+L EI F+GK+ ++ LD + K G
Sbjct: 103 PYFATLTLGPEIYFVGKSRSMWILDSRSGKLRQG 136
>At3g44160.1 68416.m04734 chloroplast outer membrane
protein-related low similarity to chloroplastic outer
envelope membrane protein (OEP75) [Pisum sativum]
GI:633607
Length = 362
Score = 28.7 bits (61), Expect = 9.1
Identities = 15/37 (40%), Positives = 20/37 (54%), Gaps = 2/37 (5%)
Query: 14 LSGNKSIHDAVLKIFTSVNKVFIHQLCTWLLYGELKD 50
+ KSIH KI VN F H+LCT L++ +D
Sbjct: 1 MGAQKSIHAGRAKI--DVNVDFTHKLCTSLMFPAFRD 35
>At3g23430.1 68416.m02953 phosphate transporter, putative (PHO1)
identical to PHO1 protein [Arabidopsis thaliana]
GI:20069032; supporting cDNA gi|20069031|gb|AF474076.1|;
contains Pfam profiles PF03124: EXS family and PF03105:
SPX domain
Length = 782
Score = 28.7 bits (61), Expect = 9.1
Identities = 14/49 (28%), Positives = 26/49 (53%)
Query: 367 LQHYMQADVLETNFSRLMDAVNKTNDFEKLKKAHATFLADILSQSFLTV 415
+Q + + D ++ F+RL + +NK N F K K+ +IL + T+
Sbjct: 106 VQLFSEEDEVKVFFARLDEELNKVNQFHKPKETEFLERGEILKKQLETL 154
>At1g65930.1 68414.m07481 isocitrate dehydrogenase, putative / NADP+
isocitrate dehydrogenase, putative strong similarity to
isocitrate dehydrogenase SP|Q40345 from [Medicago
sativa]
Length = 410
Score = 28.7 bits (61), Expect = 9.1
Identities = 23/103 (22%), Positives = 45/103 (43%), Gaps = 7/103 (6%)
Query: 288 DEDYSTDGWSSIILKYDFKWPLHLLFTPEVLARYN----DMFRLLLRLK-KTQHDLHSLW 342
DE +S+ Y+ KWPL+L +L +Y+ D+F+ + K+++D +W
Sbjct: 186 DESIRAFADASMNTAYEKKWPLYLSTKNTILKKYDGRFKDIFQEVYEASWKSKYDAAGIW 245
Query: 343 KTYKQSASFAMSQLHNK--LMFLMDNLQHYMQADVLETNFSRL 383
++ L ++ ++ N +Q+D L F L
Sbjct: 246 YEHRLIDDMVAYALKSEGGYVWACKNYDGDVQSDFLAQGFGSL 288
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.321 0.135 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 10,827,781
Number of Sequences: 28952
Number of extensions: 429611
Number of successful extensions: 1120
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 9
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1096
Number of HSP's gapped (non-prelim): 27
length of query: 486
length of database: 12,070,560
effective HSP length: 84
effective length of query: 402
effective length of database: 9,638,592
effective search space: 3874713984
effective search space used: 3874713984
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.9 bits)
S2: 61 (28.7 bits)
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