Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001849-TA|BGIBMGA001849-PA|IPR001563|Peptidase S10,
serine carboxypeptidase, IPR006195|Aminoacyl-transfer RNA synthetase,
class II, IPR002320|Threonyl-tRNA synthetase, class IIa,
IPR004154|Anticodon-binding, IPR012676|TGS-like, IPR004095|TGS,
IPR012947|Threonyl/alanyl tRNA synthetase, SAD, IPR002314|tRNA
synthetase, class II (G, H, P and S)
(703 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC25H2.02 |ths1||threonine-tRNA ligase Ths1 |Schizosaccharomyc... 833 0.0
SPAC24C9.09 |||mitochondrial threonine-tRNA ligase|Schizosacchar... 367 e-102
SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|ch... 47 8e-06
SPBC1105.01 |rrp12|SPBPB7E8.03|rRNA processing protein Rrp12|Sch... 29 2.9
SPBC646.17c |dic1|SPBC855.01c, SPBP35G2.01c, mug44|dynein interm... 28 3.8
SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase |Schizosaccharo... 28 5.1
SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 28 5.1
>SPBC25H2.02 |ths1||threonine-tRNA ligase Ths1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 703
Score = 833 bits (2060), Expect = 0.0
Identities = 393/687 (57%), Positives = 510/687 (74%), Gaps = 17/687 (2%)
Query: 23 YIQKRITLWDKYKAQYTEQIASKPDISVVVTLPDGKTVEAKAWKTTPYDVAKGISQGLAD 82
+IQ R+ L+DK + +Y E +A+KP + +TLPDGK ++ +W+TTP +A IS+GLAD
Sbjct: 22 FIQHRLDLFDKLQKEYKESLATKPREEIDITLPDGKVIKGTSWETTPISIAASISKGLAD 81
Query: 83 ATIIARVNNELWDLDRPLEGDCKLELLRWDNTDAQAVFWHSSAHMLGEAMERVYGGCLCY 142
+A VN E WDL RPLE C L+L +++ + + VFWHSSAH+LGEA E + LC
Sbjct: 82 RVTVAIVNGEPWDLTRPLEASCTLKLCDFNDPEGKRVFWHSSAHILGEATELSFHCHLCI 141
Query: 143 GPPIEEGFYYDMYYPE-KGISSTDFNVLEGLIKKIAKEKQPFERLELTKEQLLEMFDYNP 201
GPP +EGF+Y+M + I++ D++ +E K+ K+KQPFERL ++KE LLEMF YN
Sbjct: 142 GPPTDEGFFYEMGIDNGRVITNDDYSSIESYAKQAIKQKQPFERLVISKEGLLEMFRYNK 201
Query: 202 FKVRILNEKVQT-PTTTVYRCGPLIDLCRGPHVRHTGKVKALKVTKNSATYWEGKAEAES 260
+K I+ K+ +TTVYRCGPLIDLC GPHV HTG++K+ VTKNS++Y+ G A+ +S
Sbjct: 202 YKQYIIQTKIPDGASTTVYRCGPLIDLCTGPHVPHTGRIKSFAVTKNSSSYFLGDAKNDS 261
Query: 261 LQRVYGISFPEPKQLKEWEVIQEEAAKRDHRKIGREQELFFFHELSPGSCFFQPRGAHIY 320
LQRVYGISFP+ KQ++E++ EAAKRDHRKIGR+QELFFF+E+SPGSCFF P GA IY
Sbjct: 262 LQRVYGISFPDNKQMQEYKTFLAEAAKRDHRKIGRDQELFFFNEISPGSCFFLPHGARIY 321
Query: 321 NTLVNFIKEQYRSRGFHEVVTPNMYNAKLWQTSGHWAHYAENMFSFDVEKETFALKPMNC 380
NTL+ +++ QY RG+ EV+TPNMYN LW+TSGHW +Y+ENMFSFD+EKE +ALKPMNC
Sbjct: 322 NTLLKYMRYQYSKRGYQEVITPNMYNVNLWKTSGHWNNYSENMFSFDIEKEKYALKPMNC 381
Query: 381 PGHCLMFDNRTRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFCAPQQIE 440
PGHC+MF +R RS+R+LP R+ADFGVLHRNE SGAL+GLTRVRRFQQDDAHIFC P Q+
Sbjct: 382 PGHCVMFKSRDRSYRDLPWRVADFGVLHRNEFSGALSGLTRVRRFQQDDAHIFCTPDQVR 441
Query: 441 AEIVGCLEFLKCVYSTFGFTFQLKLSTRP-EKYLGDLETWNQAEKALEDSLNKFGKPWQL 499
+EI GC +FLK VY TFGFTF L+LSTRP EKYLGDL TW++AE L+ +L+ G W+L
Sbjct: 442 SEIEGCFDFLKEVYGTFGFTFHLELSTRPEEKYLGDLATWDKAEAQLKAALDASGYKWEL 501
Query: 500 NPGDGAFYGPKIDITIQDALRRSHQCATIQLDFQLPERFNLTYSVVKFWCCVSFGCSETG 559
N GDGAFYGPKIDIT+ DAL+R HQCATIQLDFQLPERF L + + E+G
Sbjct: 502 NAGDGAFYGPKIDITVFDALKRQHQCATIQLDFQLPERFQLEFHAP----ASAEEAKESG 557
Query: 560 DKK---RPVIIHRAVLGSVERMVAILSESYAGKWPLWLSPRQLRVVPVGPAFDDYAVQVN 616
+ RPV++HRA+LGS+ERM+AIL+E YAGKWP W+SPRQ+ ++PV A +YA +V+
Sbjct: 558 NNNKYTRPVMVHRAILGSLERMIAILTEHYAGKWPFWMSPRQVCIIPVSAAAYNYADKVH 617
Query: 617 DKLFAAGFMSEVDTDAGD-TLNKKVRNAQLAQFNYILVVGEREKNSGTVNVRTRDN---- 671
+ L A VDTD D TL KK+RNAQLAQ+N+I VVG E+++ +VNVR RD+
Sbjct: 618 NILSEADIF--VDTDKSDNTLPKKIRNAQLAQYNFIFVVGAEEESTNSVNVRNRDDPKKQ 675
Query: 672 KVHGEMSVEALIEHLNNLVREKKLSED 698
++VE +++ L L K L D
Sbjct: 676 SKGSTVAVEEVVKKLLKLKESKSLIND 702
>SPAC24C9.09 |||mitochondrial threonine-tRNA
ligase|Schizosaccharomyces pombe|chr 1|||Manual
Length = 473
Score = 367 bits (902), Expect = e-102
Identities = 194/434 (44%), Positives = 268/434 (61%), Gaps = 33/434 (7%)
Query: 264 VYGISF-PEPKQLKEWEVIQEEAAKRDHRKIGREQELFFFHELSPGSCFFQPRGAHIYNT 322
+Y F P P K + Q E+ DHR I Q+L+ L+PGS FF P G IYN
Sbjct: 21 IYTARFGPTPFSTKANNLKQPESTLNDHRTIAARQKLYTTSILTPGSIFFLPHGTRIYNR 80
Query: 323 LVNFIKEQYRSRGFHEVVTPNMYNAKLWQTSGHWAHYAENMF----SFDVEKE----TFA 374
LV+F++ QY+ GF E++TP ++ LW+ SGHW +Y + +F S +VE E T+
Sbjct: 81 LVDFLRAQYQIHGFEEIITPLIFKKDLWEKSGHWQNYEKEIFRVEESRNVEDEHSNATYG 140
Query: 375 LKPMNCPGHCLMFDNRTRSWRELPLRLADFGVLHRNELSGALTGLTRVRRFQQDDAHIFC 434
LKPMNCPGHC+++ + RS++ELPLR ADF LHRNE SGAL+GLTR+R F QDD HIFC
Sbjct: 141 LKPMNCPGHCIVYASTERSYKELPLRFADFSPLHRNEASGALSGLTRLRCFHQDDGHIFC 200
Query: 435 APQQIEAEIVGCLEFLKCVYSTFGFT-FQLKLSTRPEKYLGDLETWNQAEKALEDSLNKF 493
+P+ I+ EI L F+K VYS G +L LSTRPE+++G L+TWN+AE L ++L +
Sbjct: 201 SPESIKDEIKNTLTFVKQVYSLLGMNKLKLYLSTRPEEHIGSLDTWNEAENGLREALQES 260
Query: 494 GKPWQLNPGDGAFYGPKIDITIQDALRRSHQCATIQLDFQLPERFNLTYSVVKFWCCVSF 553
G+ W +N GDGAFYGPKID+ + DA + HQ ATIQLDF LP+RF L Y
Sbjct: 261 GETWIINEGDGAFYGPKIDVMVADARGKWHQTATIQLDFNLPQRFKLYYR-------TDA 313
Query: 554 GCSETGDK--KRPVIIHRAVLGSVERMVAILSESYAGKWPLWLSPRQLRVVPVGPA--FD 609
G + +K K+PV+IHRA+ GS+ER + IL E G WP WLSPR ++PV
Sbjct: 314 GDNAGSEKLIKQPVMIHRAIFGSLERFMGILIEHLNGHWPFWLSPRHAVILPVNQTDRIL 373
Query: 610 DYAVQV-----NDKLFAAGFMS------EVDTDA-GDTLNKKVRNAQLAQFNYILVVGER 657
YA QV N ++ + F+ VD +A ++ K++R ++L +NY +V+GE
Sbjct: 374 TYAKQVQKELSNQEVNESEFLPLNKNTYYVDINAEAQSIGKRLRESRLLNYNYEIVIGED 433
Query: 658 EKNSGTVNVRTRDN 671
E + ++V +R N
Sbjct: 434 EVDKEILSVSSRHN 447
>SPBC24C6.03 |||proline-tRNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 425
Score = 47.2 bits (107), Expect = 8e-06
Identities = 32/137 (23%), Positives = 54/137 (39%), Gaps = 2/137 (1%)
Query: 298 ELFFFHELSPGSCFFQPRGAHIYNTLVNFIKEQYRSRGFHEVVTPNMYNAKLWQTSGHWA 357
++ F PG + P G + N + + + RS G + ++ + ++W+ SG W
Sbjct: 33 DMGFIQPSMPGIFQYLPLGLRVQNKICDLLHISMRSLGASAISLAHLSSKEIWEKSGRWQ 92
Query: 358 HYAENMFSF-DVEKETFALKPMNCPGHCLMFDNRTRSWRELPLRLADFGVLHRNELSGAL 416
+F D L P + S ++LP+R+ G R+EL
Sbjct: 93 KTGSELFRLHDRNDREMCLAPTHEEDVTRTMATIIDSQKQLPIRVYQIGRKFRDELR-PR 151
Query: 417 TGLTRVRRFQQDDAHIF 433
GL R R F D + F
Sbjct: 152 GGLLRGREFMMKDLYTF 168
Score = 35.5 bits (78), Expect = 0.025
Identities = 29/129 (22%), Positives = 58/129 (44%), Gaps = 7/129 (5%)
Query: 561 KKRPVIIHRAVLG-SVERMVA----ILSESYAGKWPLWLSPRQLRVVPVGPAFDDYAVQV 615
K + ++H G V R++A + ++ WP ++P ++ VVP A V
Sbjct: 288 KNKQEVLHMGCYGIGVSRLIAAVAHVTKDAKGLVWPSSIAPWKVLVVPTSDNHIQSAETV 347
Query: 616 NDKLF-AAGFMSEVDTDAGD-TLNKKVRNAQLAQFNYILVVGEREKNSGTVNVRTRDNKV 673
D GF + + D + K+R+A+L + +++VVG R + G + R +
Sbjct: 348 YDATANVVGFDNVLLEDRQNRAFGYKMRDAELIGYPFVIVVGSRFQEEGICEIIVRSSGE 407
Query: 674 HGEMSVEAL 682
++ ++L
Sbjct: 408 RYKLDKDSL 416
>SPBC1105.01 |rrp12|SPBPB7E8.03|rRNA processing protein
Rrp12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1001
Score = 28.7 bits (61), Expect = 2.9
Identities = 18/56 (32%), Positives = 27/56 (48%), Gaps = 1/56 (1%)
Query: 648 FNYILVVGEREKNSGTVNVRTRDNKVHGEMSVEALIEHLNNLVREKKLSEDSELLK 703
F ++ VG R KN G +N+ D + + VEA I +++ L DS LK
Sbjct: 755 FELLVEVGNRMKNGGKINMSLIDGEEPNKTVVEANINEYISMI-SANLMNDSLNLK 809
>SPBC646.17c |dic1|SPBC855.01c, SPBP35G2.01c, mug44|dynein
intermediate chain Dic1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 544
Score = 28.3 bits (60), Expect = 3.8
Identities = 19/74 (25%), Positives = 33/74 (44%), Gaps = 3/74 (4%)
Query: 28 ITLWDKYKAQYTEQIASKPDISVVVTLPDGKTVEAKAWKTTPYDVAKGISQGLADATIIA 87
+ LW ++ ++ D+ V + KT KA +DV +S+ A++ A
Sbjct: 428 VRLWQCSPSRNQHELVPSNDLDEQVIINSCKTFTHKAMV---FDVKWCVSEPCCFASVDA 484
Query: 88 RVNNELWDLDRPLE 101
N LWDL + +E
Sbjct: 485 LGNLNLWDLQKDVE 498
>SPBP4H10.11c |||long-chain-fatty-acid-CoA ligase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 689
Score = 27.9 bits (59), Expect = 5.1
Identities = 11/36 (30%), Positives = 20/36 (55%)
Query: 549 CCVSFGCSETGDKKRPVIIHRAVLGSVERMVAILSE 584
CC + TG K V++HR ++ ++ + ILS+
Sbjct: 248 CCYMYTSGSTGKPKGVVLLHRNIIAALGGINRILSQ 283
>SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 419
Score = 27.9 bits (59), Expect = 5.1
Identities = 18/51 (35%), Positives = 30/51 (58%), Gaps = 4/51 (7%)
Query: 239 VKALK-VTKNSATYWEGKAEAESLQRVYG---ISFPEPKQLKEWEVIQEEA 285
+KA K + + S +Y E + E E+L + SF K+LKE ++IQE++
Sbjct: 352 LKACKDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEKIIQEKS 402
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.319 0.136 0.419
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,413,406
Number of Sequences: 5004
Number of extensions: 149120
Number of successful extensions: 372
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 356
Number of HSP's gapped (non-prelim): 9
length of query: 703
length of database: 2,362,478
effective HSP length: 78
effective length of query: 625
effective length of database: 1,972,166
effective search space: 1232603750
effective search space used: 1232603750
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 57 (27.1 bits)
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