Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001751-TA|BGIBMGA001751-PA|IPR002048|Calcium-binding
EF-hand
(217 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g05790.1 68416.m00650 Lon protease, putative similar to Lon p... 31 0.46
At3g48070.1 68416.m05241 expressed protein 31 0.60
At1g76040.2 68414.m08829 calcium-dependent protein kinase, putat... 31 0.60
At1g76040.1 68414.m08830 calcium-dependent protein kinase, putat... 31 0.60
At3g47480.1 68416.m05163 calcium-binding EF hand family protein ... 31 0.80
At4g35310.1 68417.m05019 calcium-dependent protein kinase, putat... 30 1.1
At2g17290.1 68415.m01997 calcium-dependent protein kinase isofor... 29 1.8
At4g36120.1 68417.m05141 expressed protein 29 3.2
At1g24620.1 68414.m03097 polcalcin, putative / calcium-binding p... 28 4.2
At3g10660.1 68416.m01282 calcium-dependent protein kinase isofor... 28 5.6
At2g40620.1 68415.m05010 bZIP transcription factor family protei... 28 5.6
At2g38910.1 68415.m04783 calcium-dependent protein kinase, putat... 28 5.6
At1g50030.1 68414.m05614 target of rapamycin protein (TOR) ident... 28 5.6
At5g04870.1 68418.m00510 calcium-dependent protein kinase isofor... 27 7.4
At4g38230.1 68417.m05399 calcium-dependent protein kinase, putat... 27 7.4
At4g26470.1 68417.m03808 calcium-binding EF hand family protein ... 27 7.4
At2g41100.2 68415.m05077 touch-responsive protein / calmodulin-r... 27 7.4
At2g41100.1 68415.m05076 touch-responsive protein / calmodulin-r... 27 7.4
At1g64480.1 68414.m07310 calcineurin B-like protein 8 (CBL8) ide... 27 7.4
At5g49480.1 68418.m06123 sodium-inducible calcium-binding protei... 27 9.8
At5g41840.1 68418.m05095 F-box family protein contains F-box dom... 27 9.8
At5g22690.1 68418.m02651 disease resistance protein (TIR-NBS-LRR... 27 9.8
At2g17890.1 68415.m02072 calcium-dependent protein kinase family... 27 9.8
>At3g05790.1 68416.m00650 Lon protease, putative similar to Lon
protease homolog 2 SP:P93655
Length = 942
Score = 31.5 bits (68), Expect = 0.46
Identities = 18/38 (47%), Positives = 23/38 (60%), Gaps = 8/38 (21%)
Query: 91 HHGDP-----QLLNPANIAQERDHIQEHMDVPIDTSKM 123
HHGDP +LL+P A DH ++DVPID SK+
Sbjct: 533 HHGDPASAMLELLDPEQNANFLDH---YLDVPIDLSKV 567
>At3g48070.1 68416.m05241 expressed protein
Length = 319
Score = 31.1 bits (67), Expect = 0.60
Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Query: 137 DADNNNKLDGCELIKSLIHWHEQGHKQENPPSEAPVGEKIFNEDELVNLIDPIL 190
DAD++ LD E I + ++ H++ENPP E I + L+ D +L
Sbjct: 137 DADDDGCLDDWEAIADALAADDEKHEKENPPESCQEHEDI-KQSALIGGADVVL 189
>At1g76040.2 68414.m08829 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase GB:AAC25423 GI:3283996 [Nicotiana tabacum]
Length = 534
Score = 31.1 bits (67), Expect = 0.60
Identities = 28/107 (26%), Positives = 47/107 (43%), Gaps = 17/107 (15%)
Query: 121 SKMSDQELQFHYFKMHDADNNNKLDGCELIKSLIHWHEQGHKQENPPSEAPVGEK----I 176
SK+++ E++ + D D + +D E + + +H H K+EN +K
Sbjct: 419 SKLTESEIK-QLMEAADVDKSGTIDYIEFVTATMHRHRL-EKEENLIEAFKYFDKDRSGF 476
Query: 177 FNEDELVN-----------LIDPILNMDDHNRDGYIDYPEFVRAQQK 212
DEL + ID ++N D + DG I+Y EFV +K
Sbjct: 477 ITRDELKHSMTEYGMGDDATIDEVINDVDTDNDGRINYEEFVAMMRK 523
>At1g76040.1 68414.m08830 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase GB:AAC25423 GI:3283996 [Nicotiana tabacum]
Length = 323
Score = 31.1 bits (67), Expect = 0.60
Identities = 28/107 (26%), Positives = 47/107 (43%), Gaps = 17/107 (15%)
Query: 121 SKMSDQELQFHYFKMHDADNNNKLDGCELIKSLIHWHEQGHKQENPPSEAPVGEK----I 176
SK+++ E++ + D D + +D E + + +H H K+EN +K
Sbjct: 208 SKLTESEIK-QLMEAADVDKSGTIDYIEFVTATMHRHRL-EKEENLIEAFKYFDKDRSGF 265
Query: 177 FNEDELVN-----------LIDPILNMDDHNRDGYIDYPEFVRAQQK 212
DEL + ID ++N D + DG I+Y EFV +K
Sbjct: 266 ITRDELKHSMTEYGMGDDATIDEVINDVDTDNDGRINYEEFVAMMRK 312
>At3g47480.1 68416.m05163 calcium-binding EF hand family protein
contains INTERPRO:IPR002048 calcium-binding EF-hand
domain
Length = 183
Score = 30.7 bits (66), Expect = 0.80
Identities = 19/73 (26%), Positives = 34/73 (46%), Gaps = 1/73 (1%)
Query: 142 NKLDGCELIKSLIHWHEQGHKQENPPSEAPVGEKIFNEDELVNL-IDPILNMDDHNRDGY 200
N++ G E++K ++ +E + DE + ++ + D NRDG
Sbjct: 109 NEMMGKEIVKEAFRLFDENQDGFIDENELKHVLSLLGYDECTKMECRKMVKVYDENRDGK 168
Query: 201 IDYPEFVRAQQKS 213
ID+ EFV+ +KS
Sbjct: 169 IDFYEFVKLIEKS 181
>At4g35310.1 68417.m05019 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calmodulin-domain protein
kinase CDPK isoform 6 [Arabidopsis thaliana]
gi|1399275|gb|AAB03246; contains protein kinase domain,
Pfam:PF00069; contains EF hand domain (calcium-binding
EF-hand), Pfam:PF00036, INTERPRO:IPR002048
Length = 556
Score = 30.3 bits (65), Expect = 1.1
Identities = 13/27 (48%), Positives = 16/27 (59%)
Query: 186 IDPILNMDDHNRDGYIDYPEFVRAQQK 212
++ I+ D N DG IDY EFV QK
Sbjct: 509 LEDIIKEVDQNNDGKIDYGEFVEMMQK 535
>At2g17290.1 68415.m01997 calcium-dependent protein kinase isoform 6
(CPK6) identical to calmodulin-domain protein kinase
CDPK isoform 6 [Arabidopsis thaliana]
gi|1399275|gb|AAB03246; contains protein kinase domain,
Pfam:PF00069; contains EF hand domain (calcium-binding
EF-hand), Pfam:PF00036, INTERPRO:IPR002048
Length = 544
Score = 29.5 bits (63), Expect = 1.8
Identities = 29/106 (27%), Positives = 46/106 (43%), Gaps = 16/106 (15%)
Query: 121 SKMSDQELQFHYFKMHDADNNNKLDGCELIKSLIHWHEQGHKQENPPSEAPVGEK----I 176
S + D E++ + D DN+ +D E I + IH ++ ++E+ S +K
Sbjct: 420 STLKDTEIR-DLMEAADVDNSGTIDYSEFIAATIHLNKL-EREEHLVSAFQYFDKDGSGY 477
Query: 177 FNEDELVN----------LIDPILNMDDHNRDGYIDYPEFVRAQQK 212
DEL ++ I+ D + DG IDY EFV QK
Sbjct: 478 ITIDELQQSCIEHGMTDVFLEDIIKEVDQDNDGRIDYEEFVAMMQK 523
>At4g36120.1 68417.m05141 expressed protein
Length = 981
Score = 28.7 bits (61), Expect = 3.2
Identities = 25/95 (26%), Positives = 41/95 (43%), Gaps = 7/95 (7%)
Query: 102 NIAQERDHIQEHMDVPIDT-SKMSDQELQFHYFKMHDADNNNKLDGCELIKSLIHWHEQG 160
+++ + +Q +V T K+ E Q H F ++ N K + L +SL G
Sbjct: 376 HLSARNNELQVSRNVCAKTLGKLKILEGQMHMF--NNDKNAPKSNSRNLSESL----SSG 429
Query: 161 HKQENPPSEAPVGEKIFNEDELVNLIDPILNMDDH 195
H PPS V E F+E+ + P ++D H
Sbjct: 430 HDHHYPPSVTSVSEDGFDEEGSSSECGPATSLDSH 464
>At1g24620.1 68414.m03097 polcalcin, putative / calcium-binding
pollen allergen, putative similar to polcalcin Jun o 2
(calcium-binding pollen allergen Jun o 2) SP:O64943 from
[Juniperus oxycedrus]
Length = 186
Score = 28.3 bits (60), Expect = 4.2
Identities = 16/39 (41%), Positives = 19/39 (48%), Gaps = 4/39 (10%)
Query: 179 EDELVNLIDPILNMDDHNRDGYIDYPEFVRAQQKSQKQN 217
E+EL I I D DGYI++ EFV K QN
Sbjct: 71 EEELEKAITEI----DRKGDGYINFEEFVELNTKGMDQN 105
>At3g10660.1 68416.m01282 calcium-dependent protein kinase isoform 2
(CPK2) identical to calcium-dependent protein kinase
isoform 2 [Arabidopsis thaliana] gi|9837343|gb|AAG00535;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 646
Score = 27.9 bits (59), Expect = 5.6
Identities = 12/27 (44%), Positives = 17/27 (62%)
Query: 186 IDPILNMDDHNRDGYIDYPEFVRAQQK 212
I+ ++ D ++DG IDY EFV QK
Sbjct: 598 IEEMMRDVDQDKDGRIDYNEFVAMMQK 624
>At2g40620.1 68415.m05010 bZIP transcription factor family protein
identical to b-Zip DNA binding protein GI:2246376 from
[Arabidopsis thaliana]; contains a bZIP transcription
factor basic domain signature (PDOC00036)
Length = 367
Score = 27.9 bits (59), Expect = 5.6
Identities = 17/80 (21%), Positives = 30/80 (37%), Gaps = 5/80 (6%)
Query: 106 ERDHIQEHMDVPIDTSKMSDQELQFHYFKMHDADNNNKLDGCELIKSLIHWHEQGHKQEN 165
++ Q H D + QFH F+ ++ N + L+H H N
Sbjct: 266 QQSFFQHHHQQQTDAQNLQQMTHQFHLFQPNNNQNQSSRTNPPTAHQLMH-----HATSN 320
Query: 166 PPSEAPVGEKIFNEDELVNL 185
P+++ + +ED L L
Sbjct: 321 APAQSHSYSEAMHEDHLGRL 340
>At2g38910.1 68415.m04783 calcium-dependent protein kinase, putative
/ CDPK, putative similar to calcium-dependent protein
kinase, isoform AK1 (CDPK) [Arabidopsis thaliana]
SWISS-PROT:Q06850; contains protein kinase domain,
Pfam:PF00069; contains EF hand domain (calcium-binding
EF-hand), Pfam:PF00036, INTERPRO:IPR002048
Length = 583
Score = 27.9 bits (59), Expect = 5.6
Identities = 13/26 (50%), Positives = 15/26 (57%)
Query: 186 IDPILNMDDHNRDGYIDYPEFVRAQQ 211
+D IL D + DG IDY EFV Q
Sbjct: 546 LDDILREVDKDNDGRIDYSEFVDMMQ 571
>At1g50030.1 68414.m05614 target of rapamycin protein (TOR) identical
to pTOR [Arabidopsis thaliana] GI:12002902; contains Pfam
profiles PF00454 Phosphatidylinositol 3- and 4-kinase,
PF02259 FAT domain, PF02260 FATC domain
Length = 2481
Score = 27.9 bits (59), Expect = 5.6
Identities = 15/47 (31%), Positives = 25/47 (53%), Gaps = 2/47 (4%)
Query: 148 ELIKSLIHWHEQGHKQENPPSEAPVGEKIFNEDELVNLIDPILNMDD 194
ELI+ I WHE H+ S GE N + ++ +++P+ +M D
Sbjct: 1920 ELIRVAILWHEMWHEALEEASRLYFGE--HNIEGMLKVLEPLHDMLD 1964
>At5g04870.1 68418.m00510 calcium-dependent protein kinase isoform
AK1 (AK1) identical to calcium-dependent protein kinase,
isoform AK1 (CDPK) [Arabidopsis thaliana]
SWISS-PROT:Q06850; contains protein kinase domain,
Pfam:PF00069; contains EF hand domain (calcium-binding
EF-hand), Pfam:PF00036, INTERPRO:IPR002048
Length = 610
Score = 27.5 bits (58), Expect = 7.4
Identities = 12/27 (44%), Positives = 16/27 (59%)
Query: 186 IDPILNMDDHNRDGYIDYPEFVRAQQK 212
I+ ++ D + DG IDY EFV QK
Sbjct: 562 IEELMRDVDQDNDGRIDYNEFVAMMQK 588
>At4g38230.1 68417.m05399 calcium-dependent protein kinase, putative
/ CDPK, putative calmodulin-domain protein kinase CDPK
isoform 6 [Arabidopsis thaliana] gi|1399275|gb|AAB03246;
contains protein kinase domain, Pfam:PF00069; contains
EF hand domain (calcium-binding EF-hand), Pfam:PF00036,
INTERPRO:IPR002048
Length = 340
Score = 27.5 bits (58), Expect = 7.4
Identities = 11/27 (40%), Positives = 16/27 (59%)
Query: 186 IDPILNMDDHNRDGYIDYPEFVRAQQK 212
++ ++ D + DG IDY EFV QK
Sbjct: 292 LEDVIKEVDQDNDGRIDYGEFVAMMQK 318
>At4g26470.1 68417.m03808 calcium-binding EF hand family protein low
similarity to SP|P06787 Calmodulin {Saccharomyces
cerevisiae}; contains INTERPRO:IPR002048 calcium-binding
EF-hand domain
Length = 248
Score = 27.5 bits (58), Expect = 7.4
Identities = 13/39 (33%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Query: 179 EDELVNLIDPILNMDDHNRDGYIDYPEFVRAQQKSQKQN 217
E L+D + +D+ N+DGY+ E VRA +S +++
Sbjct: 144 EPTFETLVDTFVFLDE-NKDGYVSREEMVRAIDESGERS 181
>At2g41100.2 68415.m05077 touch-responsive protein /
calmodulin-related protein 3, touch-induced (TCH3)
identical to calmodulin-related protein 3, touch-induced
SP:P25071 from [Arabidopsis thaliana]
Length = 235
Score = 27.5 bits (58), Expect = 7.4
Identities = 11/29 (37%), Positives = 18/29 (62%)
Query: 186 IDPILNMDDHNRDGYIDYPEFVRAQQKSQ 214
+ ++N D + DG ID+PEF+ K+Q
Sbjct: 49 LQDLMNEADLDGDGTIDFPEFLCVMAKNQ 77
>At2g41100.1 68415.m05076 touch-responsive protein /
calmodulin-related protein 3, touch-induced (TCH3)
identical to calmodulin-related protein 3, touch-induced
SP:P25071 from [Arabidopsis thaliana]
Length = 324
Score = 27.5 bits (58), Expect = 7.4
Identities = 11/29 (37%), Positives = 18/29 (62%)
Query: 186 IDPILNMDDHNRDGYIDYPEFVRAQQKSQ 214
+ ++N D + DG ID+PEF+ K+Q
Sbjct: 49 LQDLMNEADLDGDGTIDFPEFLCVMAKNQ 77
>At1g64480.1 68414.m07310 calcineurin B-like protein 8 (CBL8)
identical to calcineurin B-like protein 8 (GI:15866276)
[Arabidopsis thaliana]; similar to CALCINEURIN B SUBUNIT
GB:P25296 from [Saccharomyces cerevisiae]
Length = 214
Score = 27.5 bits (58), Expect = 7.4
Identities = 13/35 (37%), Positives = 20/35 (57%), Gaps = 1/35 (2%)
Query: 176 IFNEDELVNLI-DPILNMDDHNRDGYIDYPEFVRA 209
+F + NL D + M D R+G I++ EFVR+
Sbjct: 65 LFRNGSMQNLFADRVFYMFDRKRNGVIEFGEFVRS 99
>At5g49480.1 68418.m06123 sodium-inducible calcium-binding protein
(ACP1) / sodium-responsive calcium-binding protein
(ACP1) identical to NaCl-inducible Ca2+-binding protein
GI:2352828 from [Arabidopsis thaliana]
Length = 160
Score = 27.1 bits (57), Expect = 9.8
Identities = 13/36 (36%), Positives = 23/36 (63%), Gaps = 5/36 (13%)
Query: 171 PVGEKIFNEDELVNLIDPILNMDDHNRDGYIDYPEF 206
P GE N DE +I ++++ D N+DG++++ EF
Sbjct: 47 PSGE---NNDE--TMIGTMISVADANKDGFVEFDEF 77
>At5g41840.1 68418.m05095 F-box family protein contains F-box domain
Pfam:PF00646
Length = 540
Score = 27.1 bits (57), Expect = 9.8
Identities = 13/42 (30%), Positives = 20/42 (47%)
Query: 113 HMDVPIDTSKMSDQELQFHYFKMHDADNNNKLDGCELIKSLI 154
H+DV S + L YFK+ + N L GC ++ L+
Sbjct: 170 HIDVEGGVSLPKLKTLHLDYFKIETSMLNKLLSGCHALEELV 211
>At5g22690.1 68418.m02651 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1008
Score = 27.1 bits (57), Expect = 9.8
Identities = 10/32 (31%), Positives = 17/32 (53%)
Query: 91 HHGDPQLLNPANIAQERDHIQEHMDVPIDTSK 122
HH DP +L P E +H E+ + ++T +
Sbjct: 946 HHNDPNILAPNCGCPETEHSDEYGEFGVETKR 977
>At2g17890.1 68415.m02072 calcium-dependent protein kinase family
protein / CDPK family protein contains Pfam domains,
PF00069: Protein kinase domain and PF00036: EF hand
Length = 571
Score = 27.1 bits (57), Expect = 9.8
Identities = 11/21 (52%), Positives = 14/21 (66%)
Query: 189 ILNMDDHNRDGYIDYPEFVRA 209
IL D N DG++D+ EFV A
Sbjct: 456 ILQAIDSNTDGFVDFGEFVAA 476
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.136 0.413
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,120,511
Number of Sequences: 28952
Number of extensions: 163966
Number of successful extensions: 538
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 16
Number of HSP's successfully gapped in prelim test: 7
Number of HSP's that attempted gapping in prelim test: 509
Number of HSP's gapped (non-prelim): 36
length of query: 217
length of database: 12,070,560
effective HSP length: 78
effective length of query: 139
effective length of database: 9,812,304
effective search space: 1363910256
effective search space used: 1363910256
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 57 (27.1 bits)
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