Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001728-TA|BGIBMGA001728-PA|IPR011013|Galactose
mutarotase-like, IPR011682|Glycosyl hydrolases 38, C-terminal
(673 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At5g66150.1 68418.m08333 glycosyl hydrolase family 38 protein si... 113 5e-25
At3g26720.1 68416.m03341 glycosyl hydrolase family 38 protein si... 91 3e-18
At5g13980.2 68418.m01635 glycosyl hydrolase family 38 protein si... 87 5e-17
At5g13980.1 68418.m01634 glycosyl hydrolase family 38 protein si... 87 5e-17
At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein si... 38 0.022
At5g60900.1 68418.m07640 lectin protein kinase family protein co... 31 1.9
At2g47130.1 68415.m05886 short-chain dehydrogenase/reductase (SD... 31 3.3
At2g30800.1 68415.m03755 DEIH-box RNA/DNA helicase, putative sim... 30 5.7
At5g18370.1 68418.m02161 disease resistance protein (TIR-NBS-LRR... 29 7.6
>At5g66150.1 68418.m08333 glycosyl hydrolase family 38 protein similar
to lysosomal alpha-mannosidase SP:O09159 from [Mus
musculus]
Length = 1047
Score = 113 bits (271), Expect = 5e-25
Identities = 122/450 (27%), Positives = 205/450 (45%), Gaps = 80/450 (17%)
Query: 241 LALDVQFYYYASDKPTDTGRTDKKNPGVYLFRPVDSNPIPILD---------FI------ 285
+ +D +++YAS+ D + G Y+FRP S P+ FI
Sbjct: 638 IKVDQNYFWYASNVGD---AKDPQVSGAYIFRPNGSLAYPVSSSKICTVTSAFIGNGNVQ 694
Query: 286 -ETIVYKTRVVEEIHCAYSDYVSFVARLYTDLPYIEVDWVVGPVPVEDGW--GKDVFLRY 342
+ + + +++E+H +S +V+ V RLY + + E ++ +GP+ V G GK++ R
Sbjct: 695 SKLQIVRGPLIDEVHQQFSPWVAQVVRLYKEKEHAEFEFTIGPISVGKGHLTGKEIITRM 754
Query: 343 TTDLGNDGAFYTDANGRQTLKRLRNLRATYEPKSSKIXXXXXXXXXXXXXVTDKPFQYPC 402
TD+ FYTD+NGR LKR+R+ R + + ++P
Sbjct: 755 VTDMTTAKEFYTDSNGRDFLKRVRDNRTDWHLE------------------VNEPIA--- 793
Query: 403 FIFPGNLYPVTSKIYIEDEQKNLRFSIFNDRAQGGTSLTDGELDLMIIRRI-LTDETGTE 461
GN YP+ +YI+DE+ L S+ DRA GG S+ DGE++LM+ RR + D G E
Sbjct: 794 ----GNYYPLNLGMYIKDEKAEL--SVLVDRATGGASIKDGEIELMLHRRTSMDDSRGVE 847
Query: 462 MNLNET----EYDRGLIVRGKHILYATKMNHKPNMIYEKRLAKEISLEPKVLVSEYGGYG 517
+L ET + GL +RG + + K+ + + +EI P ++ +
Sbjct: 848 ESLVETVCVNDTCAGLTIRGNYYVSINKVGEGGR--WRRETGQEI-YSPLLMAFAHEN-- 902
Query: 518 PYLKSKW-LGETNEFSALKK--KLPLGIHLLSIEQWNEATVLIRLENYLEK-SDAIRSGI 573
K KW T + A+ LP I L+++E+ + VL+RL + E D+ S I
Sbjct: 903 ---KEKWKASNTVKGYAMDHLYTLPQNIALITLEELDLGNVLLRLAHLYEAGEDSDYSKI 959
Query: 574 KRVYLRDVFVNLRIRSIAECALGGN-VWLKDWSPLRWNKKTEFMRSFNEYYGRSNTSTVY 632
+V L+ +F I+ + E +L N +K ++W + E + + G
Sbjct: 960 AKVELKKLFSGKMIKEVTEMSLSANQEKVKMKEKMKWKVEGEAEQPSSPLRGGP------ 1013
Query: 633 ADEPRMLSEGSLEKGLTLRPQQIRTFVVWF 662
D+ ++ E L P +IRTFVV F
Sbjct: 1014 VDKSTLVVE--------LGPMEIRTFVVQF 1035
>At3g26720.1 68416.m03341 glycosyl hydrolase family 38 protein
similar to lysosomal alpha-mannosidase GI:3522867 from
[Homo sapiens]
Length = 1019
Score = 90.6 bits (215), Expect = 3e-18
Identities = 43/157 (27%), Positives = 83/157 (52%), Gaps = 5/157 (3%)
Query: 217 NKYIRINLDSTRKITNLTLSNNVFLALDVQFYYYASDKPTDTGRTDKKNPGVYLFRPVDS 276
N +R + + + +L+ N V + + YY TD D + G Y+FRP
Sbjct: 603 NLKLRYSEEGVKITRHLSTKNQV--TAEQSYAYYIGSNGTDK---DPQASGAYVFRPDGV 657
Query: 277 NPIPILDFIETIVYKTRVVEEIHCAYSDYVSFVARLYTDLPYIEVDWVVGPVPVEDGWGK 336
PI + + + + + +E+H + ++S + R+Y + E+++ +GP+P +DG K
Sbjct: 658 LPIKSKEEAQLTIVQGPLFDEVHQELNSWISQITRVYKGKNHAEIEFTIGPIPADDGISK 717
Query: 337 DVFLRYTTDLGNDGAFYTDANGRQTLKRLRNLRATYE 373
++ + TT + +G FYTD+NGR +KR+R+ R ++
Sbjct: 718 EIITKLTTTMKTNGTFYTDSNGRDFIKRIRDFRTDWD 754
Score = 77.4 bits (182), Expect = 3e-14
Identities = 62/201 (30%), Positives = 101/201 (50%), Gaps = 19/201 (9%)
Query: 407 GNLYPVTSKIYIEDEQKNLRFSIFNDRAQGGTSLTDGELDLMIIRRILTDE-TGTEMNLN 465
GN YP+ IY++D+ L S+ DRA GG+SL +G+++LM+ RR+ D+ G LN
Sbjct: 763 GNYYPLNLGIYMQDKTSEL--SVLVDRAVGGSSLENGQIELMLHRRMQHDDIRGVGEILN 820
Query: 466 ET----EYDRGLIVRGKHILYATKMNHKPNMIYEKRLAKEISLEPKVLVSEYGGYGPYLK 521
ET E +GL ++GK + K + + +EI + +E G
Sbjct: 821 ETVCLPEGCKGLTIQGKFYVQIDKPGDGAK--WRRTFGQEIYSPLLIAFTEQEG------ 872
Query: 522 SKWL-GETNEFSALKK--KLPLGIHLLSIEQWNEATVLIRLENYLE-KSDAIRSGIKRVY 577
W+ FSA + LP + LL++++ VL+RL + E D+ S + +V
Sbjct: 873 DSWINSHKTTFSAFEPSYSLPKNVALLTLQELENGEVLLRLAHLFEVGEDSEYSVMAKVE 932
Query: 578 LRDVFVNLRIRSIAECALGGN 598
L+ +F N +IR + E +L GN
Sbjct: 933 LKKLFHNKKIREVKETSLSGN 953
Score = 29.9 bits (64), Expect = 5.7
Identities = 15/23 (65%), Positives = 16/23 (69%), Gaps = 1/23 (4%)
Query: 27 TADHELVFMAENVPPLGFRSYYI 49
TA H L F A +VPPLGF SY I
Sbjct: 552 TAKHVLAFTA-SVPPLGFSSYVI 573
>At5g13980.2 68418.m01635 glycosyl hydrolase family 38 protein
similar to alpha-mannosidase GI:1419374 from [Homo
sapiens]
Length = 1024
Score = 86.6 bits (205), Expect = 5e-17
Identities = 40/126 (31%), Positives = 75/126 (59%)
Query: 248 YYYASDKPTDTGRTDKKNPGVYLFRPVDSNPIPILDFIETIVYKTRVVEEIHCAYSDYVS 307
YY A + D +N G Y+FRP + PI + V +V+E+H + ++S
Sbjct: 632 YYSAYNGSNDKEPLIPQNSGAYVFRPNGTFPINPEGQVPLTVIHGPLVDEVHQQINPWIS 691
Query: 308 FVARLYTDLPYIEVDWVVGPVPVEDGWGKDVFLRYTTDLGNDGAFYTDANGRQTLKRLRN 367
+ R+Y ++EV+++VG +P++DG GK+V + ++ L ++ FYTD++GR +KR+R+
Sbjct: 692 QITRVYKGKEHVEVEFIVGNIPIDDGIGKEVVTQISSSLKSNKTFYTDSSGRDYIKRIRD 751
Query: 368 LRATYE 373
R+ ++
Sbjct: 752 YRSDWK 757
Score = 81.4 bits (192), Expect = 2e-15
Identities = 63/200 (31%), Positives = 104/200 (52%), Gaps = 17/200 (8%)
Query: 407 GNLYPVTSKIYIEDEQKNLRFSIFNDRAQGGTSLTDGELDLMIIRRILTDET-GTEMNLN 465
GN YP+ IY++D +K FS+ DRA GG+S+ DG+++LM+ RR+L D++ G NLN
Sbjct: 766 GNYYPINHGIYLQDSKKE--FSVMVDRAFGGSSIVDGQVELMLHRRLLLDDSRGVAENLN 823
Query: 466 ET----EYDRGLIVRGKHILYATKMNHKPNMIYEKRLAKEISLEPKVLVSEYGGYGPYLK 521
ET + GL ++GK+ Y + + + +EI P +L G K
Sbjct: 824 ETVCVQDKCTGLTIQGKY--YYRIDPYGEGAKWRRTFGQEI-YSPLLLAFAQQDDG---K 877
Query: 522 SKWLGETNEFSAL--KKKLPLGIHLLSIEQWNEATVLIRLENYLE-KSDAIRSGIKRVYL 578
G + FS + LP + LL++++ ++ VL+RL + E + D SG+ V L
Sbjct: 878 PMSFGAAS-FSGIDPSYSLPDNVALLTLQELDDGNVLLRLAHLYEVEEDKELSGVASVEL 936
Query: 579 RDVFVNLRIRSIAECALGGN 598
+ +F +I + E +L N
Sbjct: 937 KKLFPGKKIGKLTEMSLSAN 956
>At5g13980.1 68418.m01634 glycosyl hydrolase family 38 protein
similar to alpha-mannosidase GI:1419374 from [Homo
sapiens]
Length = 921
Score = 86.6 bits (205), Expect = 5e-17
Identities = 40/126 (31%), Positives = 75/126 (59%)
Query: 248 YYYASDKPTDTGRTDKKNPGVYLFRPVDSNPIPILDFIETIVYKTRVVEEIHCAYSDYVS 307
YY A + D +N G Y+FRP + PI + V +V+E+H + ++S
Sbjct: 632 YYSAYNGSNDKEPLIPQNSGAYVFRPNGTFPINPEGQVPLTVIHGPLVDEVHQQINPWIS 691
Query: 308 FVARLYTDLPYIEVDWVVGPVPVEDGWGKDVFLRYTTDLGNDGAFYTDANGRQTLKRLRN 367
+ R+Y ++EV+++VG +P++DG GK+V + ++ L ++ FYTD++GR +KR+R+
Sbjct: 692 QITRVYKGKEHVEVEFIVGNIPIDDGIGKEVVTQISSSLKSNKTFYTDSSGRDYIKRIRD 751
Query: 368 LRATYE 373
R+ ++
Sbjct: 752 YRSDWK 757
Score = 68.5 bits (160), Expect = 1e-11
Identities = 53/165 (32%), Positives = 87/165 (52%), Gaps = 16/165 (9%)
Query: 407 GNLYPVTSKIYIEDEQKNLRFSIFNDRAQGGTSLTDGELDLMIIRRILTDET-GTEMNLN 465
GN YP+ IY++D +K FS+ DRA GG+S+ DG+++LM+ RR+L D++ G NLN
Sbjct: 766 GNYYPINHGIYLQDSKKE--FSVMVDRAFGGSSIVDGQVELMLHRRLLLDDSRGVAENLN 823
Query: 466 ET----EYDRGLIVRGKHILYATKMNHKPNMIYEKRLAKEISLEPKVLVSEYGGYGPYLK 521
ET + GL ++GK+ Y + + + +EI P +L G K
Sbjct: 824 ETVCVQDKCTGLTIQGKY--YYRIDPYGEGAKWRRTFGQEI-YSPLLLAFAQQDDG---K 877
Query: 522 SKWLGETNEFSAL--KKKLPLGIHLLSIEQWNEATVLIRLENYLE 564
G + FS + LP + LL++++ ++ VL+RL + E
Sbjct: 878 PMSFGAAS-FSGIDPSYSLPDNVALLTLQELDDGNVLLRLAHLYE 921
>At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein
similar to alpha-mannosidase II SP:P27046 from [Mus
musculus]
Length = 1173
Score = 37.9 bits (84), Expect = 0.022
Identities = 18/51 (35%), Positives = 32/51 (62%), Gaps = 1/51 (1%)
Query: 407 GNLYPVTSKIYIEDEQKNLRFSIFNDRAQGGTSLTDGELDLMIIRRILTDE 457
GN YP+ S +I+ RFS+ + ++ G SL +G L++M+ RR++ D+
Sbjct: 917 GNYYPMPSLAFIQGSNGQ-RFSVHSRQSLGVASLKEGWLEIMLDRRLVRDD 966
Score = 31.5 bits (68), Expect = 1.9
Identities = 12/29 (41%), Positives = 19/29 (65%)
Query: 336 KDVFLRYTTDLGNDGAFYTDANGRQTLKR 364
+++ +RY TD+ N FY+D NG Q +R
Sbjct: 879 RELIVRYKTDVDNKKVFYSDLNGFQMSRR 907
>At5g60900.1 68418.m07640 lectin protein kinase family protein
contains Pfam domains, PF01453: Lectin (probable mannose
binding) and PF00069: Protein kinase domain
Length = 748
Score = 31.5 bits (68), Expect = 1.9
Identities = 20/54 (37%), Positives = 26/54 (48%), Gaps = 2/54 (3%)
Query: 580 DVFVNLRIRSIAECALGGNVWLK-DWSPLRWNKKTEFMRSFNEYYGRSNTSTVY 632
D F+ +R RSIA+ + GN K DW + + E R F E GR VY
Sbjct: 410 DTFIKVRNRSIADVPVTGNRAKKLDW-VFTYGELAEATRDFTEELGRGAFGIVY 462
>At2g47130.1 68415.m05886 short-chain dehydrogenase/reductase (SDR)
family protein similar to
3-beta-hydroxysteroiddehydrogenase GI:15983819 from
[Digitalis lanata]
Length = 257
Score = 30.7 bits (66), Expect = 3.3
Identities = 21/77 (27%), Positives = 37/77 (48%), Gaps = 3/77 (3%)
Query: 443 GELDLMIIRRILTDETGTEMNLNETEYDRGLIVRGKHILYATKMNHKPNMIYEKRLAKEI 502
G+LD++ + ++ G+ ++LN ++DR + V + A + H + EK I
Sbjct: 82 GKLDVLFSNAGVMEQPGSFLDLNLEQFDRTMAVNVRGA--AAFIKHAARAMVEKGTRGSI 139
Query: 503 SLEPKVLVSEYGGYGPY 519
V SE GG GP+
Sbjct: 140 VCTTSV-ASEIGGPGPH 155
>At2g30800.1 68415.m03755 DEIH-box RNA/DNA helicase, putative
similar to DEIH-box RNA/DNA helicase [Arabidopsis
thaliana] GI:5881579; contains Pfam profiles PF04408:
Helicase associated domain (HA2), PF00271: Helicase
conserved C-terminal domain
Length = 1299
Score = 29.9 bits (64), Expect = 5.7
Identities = 26/85 (30%), Positives = 40/85 (47%), Gaps = 5/85 (5%)
Query: 401 PCFIFPGNLYPVTSKIYIEDEQKNLRFSIFNDRAQGGTSLTDGELDLMIIRRILTDETGT 460
P PG YPV + +Y+ED L+ N + S++D +LDL ++ DE
Sbjct: 361 PVVRVPGFTYPVRT-LYLEDVLSILKSGGDNHLSSTNLSISDHKLDLTDEDKLALDEAII 419
Query: 461 EMNLNETEYDRGLIV---RGKHILY 482
N+ E+D L + RG H +Y
Sbjct: 420 LAWTND-EFDALLDLVSSRGSHEIY 443
>At5g18370.1 68418.m02161 disease resistance protein (TIR-NBS-LRR
class), putative domain signature TIR-NBS-LRR exists,
suggestive of a disease resistance protein.
Length = 1210
Score = 29.5 bits (63), Expect = 7.6
Identities = 29/89 (32%), Positives = 47/89 (52%), Gaps = 13/89 (14%)
Query: 488 HKPNMIYEKRL-AKEISLEPKVLVSEYGGYGPYLKSKWLGETNEFSALKKKLPLGIHLL- 545
H+ N +Y+ L A + +L+ L + +G PY K L EF+AL +LPLG+ +L
Sbjct: 396 HEINHVYKVDLPATDEALQIFCLYA-FGQKFPYDGFKKLAR--EFTALAGELPLGLRVLG 452
Query: 546 ------SIEQWNEATVLIR--LENYLEKS 566
S+E+W A +R L+ +EK+
Sbjct: 453 SYLRGMSLEEWKNALPRLRTSLDGEIEKT 481
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.318 0.136 0.402
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 15,039,520
Number of Sequences: 28952
Number of extensions: 621287
Number of successful extensions: 1185
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 4
Number of HSP's that attempted gapping in prelim test: 1159
Number of HSP's gapped (non-prelim): 21
length of query: 673
length of database: 12,070,560
effective HSP length: 86
effective length of query: 587
effective length of database: 9,580,688
effective search space: 5623863856
effective search space used: 5623863856
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 63 (29.5 bits)
- SilkBase 1999-2023 -