Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001727-TA|BGIBMGA001727-PA|IPR000602|Glycoside
hydrolase, family 38
(322 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At3g26720.1 68416.m03341 glycosyl hydrolase family 38 protein si... 143 1e-34
At5g66150.1 68418.m08333 glycosyl hydrolase family 38 protein si... 136 2e-32
At5g13980.2 68418.m01635 glycosyl hydrolase family 38 protein si... 127 1e-29
At5g13980.1 68418.m01634 glycosyl hydrolase family 38 protein si... 127 1e-29
At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein si... 85 6e-17
At3g23605.1 68416.m02969 UBX domain-containing protein contains ... 32 0.59
At3g14830.1 68416.m01873 expressed protein 31 1.4
At5g65860.1 68418.m08289 ankyrin repeat family protein contains ... 29 5.5
>At3g26720.1 68416.m03341 glycosyl hydrolase family 38 protein
similar to lysosomal alpha-mannosidase GI:3522867 from
[Homo sapiens]
Length = 1019
Score = 143 bits (347), Expect = 1e-34
Identities = 108/309 (34%), Positives = 150/309 (48%), Gaps = 43/309 (13%)
Query: 2 VYTLLRQGRLFIVGGAWGMVDEATTNYHAVIDQFTYSLRKLNATFLECGRPLMAWQADVY 61
V L+ G+L + G M DEAT +Y +IDQ T + + F + P + WQ D +
Sbjct: 114 VKKLVDSGQLEFINGGMCMHDEATPHYIDMIDQTTLGHQFIKTEFGQV--PRVGWQIDPF 171
Query: 62 GHSREIASLL-AQMGFDGLFINPISFDDELDRMRRKSMEFLWRGSDDLGAETDIYTHKLF 120
GHS A LL A+ GFD LF I + D R+R K++E +W+GS LG+ + I+T +F
Sbjct: 172 GHSAVQAYLLGAEFGFDSLFFARIDYQDRAKRLREKTLEVIWQGSKSLGSSSQIFT-GVF 230
Query: 121 DGYWSPP-GFCF------GSYCDDPLLITSDSVFRNIDERVDLFIKSILHRQAPYYSTRN 173
++ PP GF F DDPLL N+ ERV+ F+ + L Q T +
Sbjct: 231 PRHYDPPEGFTFEINDVSAPIQDDPLLFD-----YNVQERVNDFVAAAL-AQVNVTRTNH 284
Query: 174 VMVMMGQRFGYYDASIWFANVDKLIKAESPLPHVLPDVPLKWSFLYSEVNKKSYETGANI 233
+M +MG F Y A WF +DK I H VNK +
Sbjct: 285 IMWLMGTDFRYQYAYSWFRQIDKFI-------HY--------------VNK-----DGRL 318
Query: 234 NLYYSTPACYLKAVYDSNPTLDTKQDDFFPMAYDKFSHATGLYTSRPTIKYMAREGHVYL 293
N+ YSTP+ Y A Y +N + K DDFFP A ++ TG +TSRP K R+ Y
Sbjct: 319 NVLYSTPSIYTDAKYAANESWPLKTDDFFPYADKPNAYWTGYFTSRPAFKKYVRDLSGYY 378
Query: 294 QMAKQLQVL 302
A+QL+ L
Sbjct: 379 LAARQLEFL 387
>At5g66150.1 68418.m08333 glycosyl hydrolase family 38 protein
similar to lysosomal alpha-mannosidase SP:O09159 from
[Mus musculus]
Length = 1047
Score = 136 bits (329), Expect = 2e-32
Identities = 105/304 (34%), Positives = 143/304 (47%), Gaps = 33/304 (10%)
Query: 2 VYTLLRQGRLFIVGGAWGMVDEATTNYHAVIDQFTYSLRKLNATFLECGRPLMAWQADVY 61
V L++ G+L V G W M DEAT +Y +IDQ T R + F P AWQ D +
Sbjct: 123 VRRLVKSGQLEFVNGGWAMNDEATCHYIDMIDQTTKGHRFIKQQFNTT--PRAAWQIDPF 180
Query: 62 GHSREIASLL-AQMGFDGLFINPISFDDELDRMRRKSMEFLWRGSDDLGAETDIYTHKLF 120
GHS A LL A++G D + I + D R KS+E +WRGS L + + I+T+ F
Sbjct: 181 GHSSVQAYLLGAELGLDSVHFARIDYQDREKRKAEKSLEVIWRGSKTLDSSSQIFTNIFF 240
Query: 121 DGYWSPPGFCFGSYCDDPLLITSDSVF--RNIDERVDLFIKSILHRQAPYYSTRNVMVMM 178
Y P GF + DD + + + F NI E V+ F+ + L A +VM M
Sbjct: 241 VHYGPPTGFHY-EVTDDYVPLQDNPRFDGYNIKEAVNDFVNASL-VYANVSRGNHVMWTM 298
Query: 179 GQRFGYYDASIWFANVDKLIKAESPLPHVLPDVPLKWSFLYSEVNKKSYETGANINLYYS 238
G F Y A WF +D+LI H VNK +N YS
Sbjct: 299 GDDFQYQFAESWFRQMDRLI-------HY--------------VNK-----DGRVNALYS 332
Query: 239 TPACYLKAVYDSNPTLDTKQDDFFPMAYDKFSHATGLYTSRPTIKYMAREGHVYLQMAKQ 298
TP+ Y+ A +N T K DFFP A +++ TG +TSRP +K R Y A+Q
Sbjct: 333 TPSLYVDAKNVANVTWPLKTHDFFPYADRAYAYWTGYFTSRPALKRYVRALSGYYMAARQ 392
Query: 299 LQVL 302
L+ L
Sbjct: 393 LEFL 396
>At5g13980.2 68418.m01635 glycosyl hydrolase family 38 protein
similar to alpha-mannosidase GI:1419374 from [Homo
sapiens]
Length = 1024
Score = 127 bits (306), Expect = 1e-29
Identities = 97/303 (32%), Positives = 142/303 (46%), Gaps = 32/303 (10%)
Query: 1 MVYTLLRQGRLFIVGGAWGMVDEATTNYHAVIDQFTYSLRKLNATFLECGRPLMAWQADV 60
+V L+ G+L ++ G M DEA +Y +IDQ T R + F P + WQ D
Sbjct: 112 IVKELIHSGQLELINGGMCMHDEAAPHYIDMIDQTTLGHRFIIREFNVT--PRIGWQIDP 169
Query: 61 YGHSREIASLL-AQMGFDGLFINPISFDDELDRMRRKSMEFLWRGSDDLGAETDIYTHKL 119
+GHS A LL A++GFD +F I + D R + K++E +WRGS LG+ + I+
Sbjct: 170 FGHSAVQAYLLGAEVGFDSVFFGRIDYQDREKRYKEKTLEVIWRGSKSLGSSSQIFAGAF 229
Query: 120 FDGYWSPPGFCFGSYCDDPLLITSD-SVF-RNIDERVDLFIKSILHRQAPYYSTRNVMVM 177
Y PPG + DD ++ D +F N+ ERV+ F+ + L QA ++M
Sbjct: 230 PTNYEPPPGGFYYEITDDSPVVQDDPDLFDYNVQERVNAFVAAALD-QANITRINHIMFT 288
Query: 178 MGQRFGYYDASIWFANVDKLIKAESPLPHVLPDVPLKWSFLYSEVNKKSYETGANINLYY 237
MG F Y A W+ +DKLI H + L VN Y T
Sbjct: 289 MGTDFRYQYAHTWYRQMDKLI-------HYVN--------LDGRVN-AFYST-------- 324
Query: 238 STPACYLKAVYDSNPTLDTKQDDFFPMAYDKFSHATGLYTSRPTIKYMAREGHVYLQMAK 297
P+ Y A + +N K +D+FP A ++ TG +TSRP +K R Y A+
Sbjct: 325 --PSIYTDAKHAANEAWPLKTEDYFPYADRINAYWTGYFTSRPALKRYVRVMSAYYLAAR 382
Query: 298 QLQ 300
QL+
Sbjct: 383 QLE 385
>At5g13980.1 68418.m01634 glycosyl hydrolase family 38 protein
similar to alpha-mannosidase GI:1419374 from [Homo
sapiens]
Length = 921
Score = 127 bits (306), Expect = 1e-29
Identities = 97/303 (32%), Positives = 142/303 (46%), Gaps = 32/303 (10%)
Query: 1 MVYTLLRQGRLFIVGGAWGMVDEATTNYHAVIDQFTYSLRKLNATFLECGRPLMAWQADV 60
+V L+ G+L ++ G M DEA +Y +IDQ T R + F P + WQ D
Sbjct: 112 IVKELIHSGQLELINGGMCMHDEAAPHYIDMIDQTTLGHRFIIREFNVT--PRIGWQIDP 169
Query: 61 YGHSREIASLL-AQMGFDGLFINPISFDDELDRMRRKSMEFLWRGSDDLGAETDIYTHKL 119
+GHS A LL A++GFD +F I + D R + K++E +WRGS LG+ + I+
Sbjct: 170 FGHSAVQAYLLGAEVGFDSVFFGRIDYQDREKRYKEKTLEVIWRGSKSLGSSSQIFAGAF 229
Query: 120 FDGYWSPPGFCFGSYCDDPLLITSD-SVF-RNIDERVDLFIKSILHRQAPYYSTRNVMVM 177
Y PPG + DD ++ D +F N+ ERV+ F+ + L QA ++M
Sbjct: 230 PTNYEPPPGGFYYEITDDSPVVQDDPDLFDYNVQERVNAFVAAALD-QANITRINHIMFT 288
Query: 178 MGQRFGYYDASIWFANVDKLIKAESPLPHVLPDVPLKWSFLYSEVNKKSYETGANINLYY 237
MG F Y A W+ +DKLI H + L VN Y T
Sbjct: 289 MGTDFRYQYAHTWYRQMDKLI-------HYVN--------LDGRVN-AFYST-------- 324
Query: 238 STPACYLKAVYDSNPTLDTKQDDFFPMAYDKFSHATGLYTSRPTIKYMAREGHVYLQMAK 297
P+ Y A + +N K +D+FP A ++ TG +TSRP +K R Y A+
Sbjct: 325 --PSIYTDAKHAANEAWPLKTEDYFPYADRINAYWTGYFTSRPALKRYVRVMSAYYLAAR 382
Query: 298 QLQ 300
QL+
Sbjct: 383 QLE 385
>At5g14950.1 68418.m01754 glycosyl hydrolase family 38 protein
similar to alpha-mannosidase II SP:P27046 from [Mus
musculus]
Length = 1173
Score = 85.0 bits (201), Expect = 6e-17
Identities = 82/303 (27%), Positives = 131/303 (43%), Gaps = 31/303 (10%)
Query: 5 LLRQGRLFIVGGAWGMVDEATTNYHAVIDQFTYSLRKLNATFLECGRPLMAWQADVYGHS 64
L++ G+L IVGG W M DEA ++Y A+I+Q LN T P +W D +G+S
Sbjct: 224 LVKDGQLEIVGGGWVMNDEANSHYFAIIEQIAEGNMWLNDTIGVI--PKNSWAIDPFGYS 281
Query: 65 REIASLLAQMGFDGLFINPISFDDELDRMRRKSMEFLWRGSDDLGAETDIYTHKL-FDGY 123
+A LL +MGF+ + I ++ + D + K++E++WR S D TDI+ H + F Y
Sbjct: 282 STMAYLLRRMGFENMLIQRTHYELKKDLAQHKNLEYIWRQSWDAMETTDIFVHMMPFYSY 341
Query: 124 WSP------PGFC----FGSYCDDPLLITS------DSVFRNIDERVDLFIKSILHRQAP 167
P P C F + ++ N+ ER L + +++
Sbjct: 342 DIPHTCGPEPAICCQFDFARMRGFKYELCPWGKHPVETTLENVQERA-LKLLDQYRKKST 400
Query: 168 YYSTRNVMVMMGQRFGYY---DASIWFANVDKLIKAESPLPHVLPDVPLKWSFLYSEVNK 224
Y T +++ +G F Y +A F N L + P + + K+ L + +
Sbjct: 401 LYRTNTLLIPLGDDFRYISIDEAEAQFRNYQMLFDHINSNPSL--NAEAKFGTL-EDYFR 457
Query: 225 KSYETGANINLYYSTPACYLKAVYDSNPTLDTKQDDFFPMAYDKFSHATGLYTSRPTIKY 284
E +N YS P P+L DFF A + + +G Y SRP K
Sbjct: 458 TVREEADRVN--YSRPGEVGSGQVVGFPSL---SGDFFTYADRQQDYWSGYYVSRPFFKA 512
Query: 285 MAR 287
+ R
Sbjct: 513 VDR 515
>At3g23605.1 68416.m02969 UBX domain-containing protein contains
Pfam profile PF00789: UBX domain
Length = 152
Score = 31.9 bits (69), Expect = 0.59
Identities = 15/45 (33%), Positives = 20/45 (44%)
Query: 211 VPLKWSFLYSEVNKKSYETGANINLYYSTPACYLKAVYDSNPTLD 255
V L WSF YS++ +S E L P Y + SN T +
Sbjct: 94 VQLLWSFCYSQIGDESSERKRRFKLIQGFPGDYKNLYFGSNTTFE 138
>At3g14830.1 68416.m01873 expressed protein
Length = 476
Score = 30.7 bits (66), Expect = 1.4
Identities = 19/37 (51%), Positives = 24/37 (64%), Gaps = 3/37 (8%)
Query: 51 RPLMAWQADVYGHSREIASLLAQMGFDGLF-INPISF 86
R L AW+ +V H +++A LAQ GF GL INP SF
Sbjct: 5 RSLEAWE-EVQRHGQDLADRLAQ-GFTGLIHINPPSF 39
>At5g65860.1 68418.m08289 ankyrin repeat family protein contains
ankyrin repeats, Pfam:PF00023
Length = 346
Score = 28.7 bits (61), Expect = 5.5
Identities = 19/77 (24%), Positives = 32/77 (41%)
Query: 6 LRQGRLFIVGGAWGMVDEATTNYHAVIDQFTYSLRKLNATFLECGRPLMAWQADVYGHSR 65
L G + VG G+VD A Y+ V + ++ +E G V+G +
Sbjct: 174 LNGGHILNVGFGMGLVDTAIQRYNPVKHTIIEAHPEVYKRMIESGWGEKENVKIVFGRWQ 233
Query: 66 EIASLLAQMGFDGLFIN 82
++ L FDG+F +
Sbjct: 234 DVLDKLDDNSFDGIFFD 250
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.324 0.139 0.440
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 8,134,205
Number of Sequences: 28952
Number of extensions: 349632
Number of successful extensions: 877
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 2
Number of HSP's that attempted gapping in prelim test: 853
Number of HSP's gapped (non-prelim): 13
length of query: 322
length of database: 12,070,560
effective HSP length: 81
effective length of query: 241
effective length of database: 9,725,448
effective search space: 2343832968
effective search space used: 2343832968
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.5 bits)
S2: 59 (27.9 bits)
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