Silkworm BLAST Result against spombe

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001725-TA|BGIBMGA001725-PA|IPR001030|Aconitate
hydratase, N-terminal
         (419 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|ch...   120   5e-28
SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr...   103   5e-23
SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyc...    69   2e-12
SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2 |Schizosacc...    62   1e-10
SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyc...    26   8.6  

>SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 778

 Score =  120 bits (288), Expect = 5e-28
 Identities = 100/333 (30%), Positives = 147/333 (44%), Gaps = 34/333 (10%)

Query: 59  EKDVSNVLDWESKQGNEESVE-IAFKPARVILQDLTGVPAVVDFAAMRDAVKDLGGDPDR 117
           EK +   LD    Q  E  V  +  +P RV  QD T   A++ F +         G P+ 
Sbjct: 63  EKILYGHLDDPVNQDIERGVSYLKLRPDRVACQDATAQMAILQFMS--------AGMPEV 114

Query: 118 INPICPADLVIDHSVQVDFARTPDALNKNQDLEFVRNKERFQFLKWGAQAFDNMLIVPPG 177
             P+    +  DH ++       D    N     V NKE + FL+     + N+    PG
Sbjct: 115 AVPVT---VHCDHLIEAYEGGPIDLERAN-----VTNKEVYDFLQTACAKY-NIGFWRPG 165

Query: 178 SGIVHQVNLEYLARVVFTGPLLHPDSVVGTDSHTTMINXXXXXXXXXXXIEAEAVMLGQA 237
           SGI+HQ+ LE  A   F G LL     +GTDSHT                +A  VM    
Sbjct: 166 SGIIHQIVLENYA---FPGGLL-----IGTDSHTPNAGGLGMVAIGVGGADAVDVMANLP 217

Query: 238 ISMLLPKVVGYKLVGELNPLATSTDLVLTITKHLRSLGVVGKFVEFFGPGVGALSIADRA 297
             +  PKV+G KL G+L    +  D++L +   L   G  G  VE+FGPGV +LS     
Sbjct: 218 WELKCPKVIGVKLTGQLKGWTSPKDVILKVAGILTVKGGTGAIVEYFGPGVESLSCTGMG 277

Query: 298 TVANMCPEFGATLAHFPVDARSLEYLHQTNRPKEKIDVIESYLRATKQFRDYTNPEQDPV 357
           T+ NM  E GAT + FP + R  EYL  TNR       I  Y    ++F      +++  
Sbjct: 278 TICNMGAEIGATTSIFPFNPRMSEYLRATNR-----SAIADY---AEEFAPIIAADENAH 329

Query: 358 FSEVVELDLGDVVTSVSGPKRPQDRVSVAIMKK 390
           + +++E+DL  +   ++GP  P     ++  K+
Sbjct: 330 YDQIIEIDLNTLEPHLNGPFTPDLATPISKFKE 362


>SPBP4H10.15 |||aconitate hydratase|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 905

 Score =  103 bits (247), Expect = 5e-23
 Identities = 77/276 (27%), Positives = 126/276 (45%), Gaps = 20/276 (7%)

Query: 146 NQDL--EFVRNKERFQFLKWGAQAFDNMLIVPPGSGIVHQVNLEYLARVVFTGPLLHPDS 203
           N D+      NKE F FL+  A+ +  +    PGSGI+HQ+ LE  A     G ++    
Sbjct: 137 NSDIPDSIANNKEIFDFLQSAAKKY-GIQFWGPGSGIIHQIVLENYAA---PGGMM---- 188

Query: 204 VVGTDSHTTMINXXXXXXXXXXXIEAEAVMLGQAISMLLPKVVGYKLVGELNPLATSTDL 263
            +GTDSHT                +A   M      +  PK++G  L G ++   T  DL
Sbjct: 189 -LGTDSHTPNAGGLGMIAIGVGGADAVDAMTNTPWELKAPKIIGVNLTGAMSGWTTPKDL 247

Query: 264 VLTITKHLRSLGVVGKFVEFFGPGVGALSIADRATVANMCPEFGATLAHFPVDARSLEYL 323
           +L +   L   G  G  +E+FGPGV +LS    ATV NM  E GAT + FP       YL
Sbjct: 248 ILHLAGKLTVRGGTGHIIEYFGPGVASLSCTGMATVCNMGAEVGATTSIFPYTDSMRRYL 307

Query: 324 HQTNRPKEKIDVIESYLRATKQFRDYTNPEQDPVFSEVVELDLGDVVTSVSGPKRPQDRV 383
             T+R     +V ++      ++ +Y   +    + ++++++L ++  S++GP  P   +
Sbjct: 308 IATHR----AEVADAASEVHSEY-NYLAADTGAKYDQIIDINLSELTPSLNGPFTPD--L 360

Query: 384 SVAIMKKDFQDCLTNKPVPIAILSGVQEHVLDFPYQ 419
           S  + K  F + +     P  + +G+     +  YQ
Sbjct: 361 STPVSK--FGEAIEKNKWPKKLSAGLIGSCTNSSYQ 394


>SPAC343.16 |lys2||homoaconitate hydratase Lys2|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 721

 Score = 68.5 bits (160), Expect = 2e-12
 Identities = 58/240 (24%), Positives = 105/240 (43%), Gaps = 21/240 (8%)

Query: 175 PPGSGIVHQVNLEYLARVVFTGPLLHPDSVVGTDSHTTMINXXXXXXXXXXXIEAEAVML 234
           P G GI HQ+ +E        G  +     V +DSH+                +A A+  
Sbjct: 156 PAGRGIGHQIMVEQ-------GYAMPGSMAVASDSHSNTYGGVGCLGTPIVRTDAAAIWA 208

Query: 235 GQAISMLLPKVVGYKLVGELNPLATSTDLVLTITKHLRSLGVVGKFVEFFGPGVGALSIA 294
                  +P +    LVG+L    +  D+++++        V+   +EF+G G+ +LSI 
Sbjct: 209 TGQTWWQIPPIARVNLVGQLPKGLSGKDIIVSLCGAFNHDEVLNHAIEFYGEGLNSLSIE 268

Query: 295 DRATVANMCPEFGATLAHFPVDARSLEYLHQ------TNRPKEKIDVIESYLRATKQFRD 348
            R T+ANM  E+GA    FP D + L +          N P+   + +++ ++A+    D
Sbjct: 269 SRLTIANMTTEWGALSGLFPTDEKLLAWYEDRLKFLGPNHPRVNRETLDA-IKASPILAD 327

Query: 349 YTNPEQDPVFSEVVELDLGDVVTSVSGPKRPQDRVSVAIMKKDFQDCLTNKPVPIAILSG 408
                +   +++ + LDL  +  +VSGP   +   S A ++K  +D L  K   ++  +G
Sbjct: 328 -----EGAFYAKHLILDLSTLSPAVSGPNSVKVYNSAATLEK--KDILIKKAYLVSCTNG 380


>SPAC9E9.03 |leu2||3-isopropylmalate dehydratase Leu2
           |Schizosaccharomyces pombe|chr 1|||Manual
          Length = 758

 Score = 62.5 bits (145), Expect = 1e-10
 Identities = 60/208 (28%), Positives = 88/208 (42%), Gaps = 14/208 (6%)

Query: 179 GIVHQVNLEYLARVVFTGPLLHPDSVVGTDSHTTMINXXXXXXXXXXXIEAEAVMLGQAI 238
           GIVH +  E        G  L   ++V  DSHT+               E E V+  Q I
Sbjct: 118 GIVHVIGPEQ-------GFTLPGTTLVCGDSHTSTHGAFGALAFGIGTSEVEHVLATQTI 170

Query: 239 SMLLPKVVGYKLVGELNPLATSTDLVLTITKHLRSLGVVGKFVEFFGPGVGALSIADRAT 298
                K +  ++ G+L     S DL+L I   + + G  G  +EF G  +  LS+  R +
Sbjct: 171 LQRKSKNMRIRVNGKLPEGIASKDLILHIIGVIGTAGGTGSVIEFCGEAIEGLSMEARMS 230

Query: 299 VANMCPEFGATLAHFPVDARSLEYLHQTNRP-KEKIDVIESYLRATKQFRDYTNPEQDPV 357
           + NM  E GA       DA + EY+   NRP   K D  E  +   K  R   N + D  
Sbjct: 231 MCNMSIEAGARAGMIAPDATTFEYV--KNRPLAPKGDDWEQAVAYWKTLRSDENAKYD-- 286

Query: 358 FSEVVELDLGDVVTSVSGPKRPQDRVSV 385
               VE++  DV+ +V+    PQD + +
Sbjct: 287 --IEVEINAADVLPTVTWGTSPQDVIPI 312


>SPBC1709.05 |sks2|hsc1|heat shock protein Sks2|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 613

 Score = 26.2 bits (55), Expect = 8.6
 Identities = 18/94 (19%), Positives = 45/94 (47%), Gaps = 2/94 (2%)

Query: 45  LESCVRNCDNFQVLEKDVSNVLDWESK-QGNEESVEIAFKPARVILQDLTGVPAVVDFAA 103
           ++  + N D F+  +KD +  L+ +S+ +    ++E       V+++   G  + ++ A 
Sbjct: 518 IQEMIENADKFKQQDKDFAKKLEAKSQLESYISNIETTISEPNVMMKLKRGDKSKIE-AQ 576

Query: 104 MRDAVKDLGGDPDRINPICPADLVIDHSVQVDFA 137
           + + +  L  +    + +  A+L +  +VQ  FA
Sbjct: 577 LAECMSQLELEDTNTDALRKAELRLKRTVQKAFA 610


  Database: spombe
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.319    0.137    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,875,530
Number of Sequences: 5004
Number of extensions: 76342
Number of successful extensions: 168
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 4
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 160
Number of HSP's gapped (non-prelim): 5
length of query: 419
length of database: 2,362,478
effective HSP length: 75
effective length of query: 344
effective length of database: 1,987,178
effective search space: 683589232
effective search space used: 683589232
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 55 (26.2 bits)