Silkworm BLAST Result against tribolium

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001703-TA|BGIBMGA001703-PA|IPR003599|Immunoglobulin
subtype, IPR003598|Immunoglobulin subtype 2,
IPR007110|Immunoglobulin-like, IPR008957|Fibronectin, type III-like
fold, IPR013151|Immunoglobulin, IPR013098|Immunoglobulin I-set
         (500 letters)

Database: tribolium 
           317 sequences; 114,650 total letters

Searching....................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

DQ157471-1|AAZ85125.1| 1639|Tribolium castaneum Down Syndrome ad...    71   2e-14
DQ659248-1|ABG47446.1|  496|Tribolium castaneum chitinase 8 prot...    25   1.2  
DQ211693-1|ABB16909.1|  314|Tribolium castaneum dorsocross protein.    23   6.5  

>DQ157471-1|AAZ85125.1| 1639|Tribolium castaneum Down Syndrome
           adhesion molecule splicevariant 3.12.3.1 protein.
          Length = 1639

 Score = 70.5 bits (165), Expect = 2e-14
 Identities = 57/212 (26%), Positives = 92/212 (43%), Gaps = 12/212 (5%)

Query: 146 QDAGDYVCQISDRVARDQVHTVEVLVPPSVRASPETRHVTARRGGAAVLECRAAGNPVPT 205
           +D+G Y+C +++ V  + V TV  +  P ++A  E +  T   G  A   C   GNP+ T
Sbjct: 300 EDSGKYLCVVNNSVGGESVETVLTVTAP-LKAKIEPQVQTIDFGRPATFTCNFEGNPIKT 358

Query: 206 VTWHRLNDSSSRLGEGPQLQLSRLERHHGGVYACTVDNGVGPPVVT-EFQLQVLY-PPEI 263
           ++W  L D          L++  + +   G+Y C + N       T E +L   + PP+I
Sbjct: 359 ISW--LKDGHPIDHNEAVLRIESVRKEDKGMYQCFIRNDQESAEATAELKLGGRFEPPQI 416

Query: 264 TVERSWVHTGEGFRAELVCTVLADPPAEVSWYQNSFPLSASER------VTMSARGSNHT 317
               +      G    L C    +P  E++W      LS SER      VT++    +H 
Sbjct: 417 RHAFNEETVQPGNSVFLKCIASGNPTPEITWELYGRRLSNSERNQIGQYVTVNGDVVSH- 475

Query: 318 LLIADVQPEDFGNYSCVADNSLGRARRHVEVS 349
           L I  +   D G Y CVA + +G A     ++
Sbjct: 476 LNITAIHTNDGGLYRCVASSKVGSADHSARIN 507



 Score = 69.3 bits (162), Expect = 6e-14
 Identities = 60/203 (29%), Positives = 87/203 (42%), Gaps = 15/203 (7%)

Query: 147 DAGDYVCQISDRV-ARDQVHTVEVLVPPSVRASPETRHVTARRGGAAVLECRAAGNPVPT 205
           D G Y C  S +V + D    + V   P VR S E + + A  GG  ++ C  AG+PV +
Sbjct: 485 DGGLYRCVASSKVGSADHSARINVYGLPFVR-SMEKQAIVA--GGTLIVHCPFAGHPVDS 541

Query: 206 VTWHR------LNDSSSRLGEGPQLQLSRLERHHG-GVYACTVDNGVGPPVVTEFQLQVL 258
           V W R      +N        G  L +  +ER      Y C   N  G       ++QV+
Sbjct: 542 VVWERDGRQLPINRKQKVFINGT-LIIENVERASDQATYDCVAKNSQGYSARGSLEVQVM 600

Query: 259 YPPEIT-VERSWVHTGEGFRAELVCTVL-ADPPAEVSWYQNSFPLSASERVTMSARGSN- 315
            PP+I   +       EG    + CTV   D P  +SW  N   +  +  + ++      
Sbjct: 601 VPPQILPFDFGEDSINEGDGVSVQCTVSKGDYPLNISWTLNGHFIDQTNGIVINRASKRV 660

Query: 316 HTLLIADVQPEDFGNYSCVADNS 338
            TL I +VQ    GNY+C+A NS
Sbjct: 661 STLSIDNVQSTHVGNYTCLASNS 683



 Score = 65.3 bits (152), Expect = 9e-13
 Identities = 89/382 (23%), Positives = 147/382 (38%), Gaps = 46/382 (12%)

Query: 149  GDYVCQISDRVARDQVHT-VEVLVPPSVRASPETRHVTARRGGAAVLECRAAGNPVPTVT 207
            G+Y C  S+  +     T + + VPP     P  +     +G  A +EC+A G P P VT
Sbjct: 674  GNYTCLASNSASVTTYTTSLFINVPPRWILEPTDKAFA--QGSDAAVECKADGFPRPVVT 731

Query: 208  WHRL-------------NDSSSRLGEGPQLQLSRLERHHGGVYACTVDNGVGPPVVTEFQ 254
            W R              N+   ++ +G  L ++ +++ + G Y C   NG+G  +    Q
Sbjct: 732  WKRATGVSPGDYKDFKPNNPDIKVEDGT-LTINNIQKTNEGYYLCEAVNGIGSGLSAVIQ 790

Query: 255  LQVLYPPEITVERSWVHTGEGFRAELVCTVLADPPAEVSWYQNSFPL--SASERVTMS-- 310
            + V  PP+  ++     +  G  A L C    + P  + W  N+  L      R T+   
Sbjct: 791  ISVQAPPQFDIKLRNQTSRRGDPAVLQCEAKGEKPIGILWNINNKRLEPKGDNRYTIREE 850

Query: 311  --ARGSNHTLLIADVQPEDFGNYSCVADNSLGRARRHVEVSGRPGP-ARFTSPPLARAPH 367
              A G    L I   +  D   ++CVA N+ G     + +  +  P   +    L ++  
Sbjct: 851  ILANGVLSGLSIKRTERSDSALFTCVATNAFGSDDTSINMIVQEVPEVPYGLKVLDKSGR 910

Query: 368  SYTLSFAV--DSYPPLDELRLLYRQLAINESFQQPGRWHDVVLAAPAAKGSLTHRVSHEL 425
            S  LS+    D   P+    + Y+Q  ++      G    V++     +  +        
Sbjct: 911  SVQLSWVAPFDGNSPITRYMIEYKQSKVS----WEGNTERVLVPGDQTEAGVF------- 959

Query: 426  RGLQPAAVYEAIVQAKNRYGWNEVSDIFQFHTL----GGPHTAHADELTPMFSAA----P 477
              L+PA  Y   + A+N  G +E S+     T     GGP T+   E     S      P
Sbjct: 960  -TLRPATTYHIRIVAENEIGSSEPSETVTIITAEEVPGGPPTSIRVETNDQHSLVVYWKP 1018

Query: 478  EPRLTQSLSVLVIYTCYWLAFS 499
              R   +  +L  Y  Y LA S
Sbjct: 1019 PAREEWNGDILGYYVGYRLANS 1040



 Score = 53.6 bits (123), Expect = 3e-09
 Identities = 58/225 (25%), Positives = 103/225 (45%), Gaps = 32/225 (14%)

Query: 146 QDAGDYVCQI-SDRVARDQVHTVEV---LVPPSVRASPETRHVTARRGGAAVLECRAAGN 201
           +D G Y C I +D+ + +    +++     PP +R +      T + G +  L+C A+GN
Sbjct: 383 EDKGMYQCFIRNDQESAEATAELKLGGRFEPPQIRHA--FNEETVQPGNSVFLKCIASGN 440

Query: 202 PVPTVTW----HRLNDSS-SRLGE--------GPQLQLSRLERHHGGVYACTVDNGVGPP 248
           P P +TW     RL++S  +++G+           L ++ +  + GG+Y C   + VG  
Sbjct: 441 PTPEITWELYGRRLSNSERNQIGQYVTVNGDVVSHLNITAIHTNDGGLYRCVASSKVG-S 499

Query: 249 VVTEFQLQVLYPPEI-TVERSWVHTGEGFRAELVCTVLADPPAEVSWYQN--SFPLSASE 305
                ++ V   P + ++E+  +  G        C     P   V W ++    P++  +
Sbjct: 500 ADHSARINVYGLPFVRSMEKQAIVAGGTLIVH--CPFAGHPVDSVVWERDGRQLPINRKQ 557

Query: 306 RVTMSARGSNHTLLIADVQ-PEDFGNYSCVADNSLG-RARRHVEV 348
           +V +     N TL+I +V+   D   Y CVA NS G  AR  +EV
Sbjct: 558 KVFI-----NGTLIIENVERASDQATYDCVAKNSQGYSARGSLEV 597



 Score = 44.0 bits (99), Expect = 2e-06
 Identities = 45/173 (26%), Positives = 67/173 (38%), Gaps = 23/173 (13%)

Query: 194 LECRAAGNPVPTVTWHRLNDSSSR-----LGE-----GPQLQLSRLERHHGGVYACTVDN 243
           L C A G P P+  W++  + ++R     L +        L +   +    G Y C V+N
Sbjct: 252 LLCPAQGFPSPSFRWYKFVEGTTRKQAVTLNDRVKQVAGTLIIREAKVEDSGKYLCVVNN 311

Query: 244 GVGPPVVTEFQLQVLYPPEITVERSWVHTGEGFRAELVCTVLADPPAEVSWYQNSFPLSA 303
            VG   V E  L V  P +  +E        G  A   C    +P   +SW ++  P+  
Sbjct: 312 SVGGESV-ETVLTVTAPLKAKIEPQVQTIDFGRPATFTCNFEGNPIKTISWLKDGHPIDH 370

Query: 304 SERVTMSARGSNHTLLIADVQPEDFGNYSCVA--DNSLGRARRHVEVSGRPGP 354
           +E V          L I  V+ ED G Y C    D     A   +++ GR  P
Sbjct: 371 NEAV----------LRIESVRKEDKGMYQCFIRNDQESAEATAELKLGGRFEP 413



 Score = 38.7 bits (86), Expect = 9e-05
 Identities = 29/96 (30%), Positives = 44/96 (45%), Gaps = 8/96 (8%)

Query: 171  VPPSVRASPETRHVTARRGGAAVLECRAAGNPVPTVTWHRLN---DSSSRLGEGP--QLQ 225
            VP  + +  +T   T +      L C A G P P +TW        +S ++ + P   L 
Sbjct: 1305 VPAKIASFDDTFTTTYKED--VTLPCLAVGLPPPVITWKIKGVQFTTSDKIRQQPDGSLF 1362

Query: 226  LSRLERHHGGVYACTVDNGVGPPVVTEFQLQVLYPP 261
            +  + R++ G Y+C V+N  G   VT  QL V  PP
Sbjct: 1363 IRDVSRNNAGEYSCHVENDYGQDSVTH-QLIVNAPP 1397



 Score = 38.3 bits (85), Expect = 1e-04
 Identities = 76/353 (21%), Positives = 132/353 (37%), Gaps = 46/353 (13%)

Query: 149  GDYVCQISDRVARDQVHTVEVLVPPSVRASPETRHVTARRGGAAVLECRAAGNPVPTVTW 208
            G Y+C+  + +       +++ V    +   + R+ T+RRG  AVL+C A G     + W
Sbjct: 771  GYYLCEAVNGIGSGLSAVIQISVQAPPQFDIKLRNQTSRRGDPAVLQCEAKGEKPIGILW 830

Query: 209  HRLNDSSSRLGEGPQ--------------LQLSRLERHHGGVYACTVDNGVGPP------ 248
            +  N      G+                 L + R ER    ++ C   N  G        
Sbjct: 831  NINNKRLEPKGDNRYTIREEILANGVLSGLSIKRTERSDSALFTCVATNAFGSDDTSINM 890

Query: 249  VVTE-----FQLQVLYPPEITVERSWVHTGEGFRAELVCTVLADPPAEVSWYQNSFPLSA 303
            +V E     + L+VL     +V+ SWV   +G  + +   ++    ++VSW  N+     
Sbjct: 891  IVQEVPEVPYGLKVLDKSGRSVQLSWVAPFDG-NSPITRYMIEYKQSKVSWEGNT----- 944

Query: 304  SERVTMSARGSNHTLLIADVQPEDFGNYSCVADNSLGRARRHVEVSGRPGPARFTSPPLA 363
             ERV +   G      +  ++P    +   VA+N +G +     V+          PP +
Sbjct: 945  -ERVLVP--GDQTEAGVFTLRPATTYHIRIVAENEIGSSEPSETVTIITAEEVPGGPPTS 1001

Query: 364  -RAPHSYTLSFAVDSYPPLDE-----LRLLYRQLAINESFQQPGRWHDVVLAAPAAKGSL 417
             R   +   S  V   PP  E     +   Y    +  S  +P  +  V       K   
Sbjct: 1002 IRVETNDQHSLVVYWKPPAREEWNGDILGYYVGYRLANS-DKPYLFETVEFGREEGK--- 1057

Query: 418  THRVSHELRGLQPAAVYEAIVQAKNRYGWNEVSDIFQFHTLGGPHTAHADELT 470
             H +  ++  L+    Y  +VQA N+ G   +SD  + +T  G      D+ T
Sbjct: 1058 EHHL--KISNLKTYTQYSVVVQAFNKVGAGPLSDDIKAYTAEGVPEQPPDKAT 1108



 Score = 36.3 bits (80), Expect = 5e-04
 Identities = 18/62 (29%), Positives = 28/62 (45%), Gaps = 3/62 (4%)

Query: 280  LVCTVLADPPAEVSWYQNSFPLSASERVTMSARGSNHTLLIADVQPEDFGNYSCVADNSL 339
            L C  +  PP  ++W       + S+++     GS   L I DV   + G YSC  +N  
Sbjct: 1326 LPCLAVGLPPPVITWKIKGVQFTTSDKIRQQPDGS---LFIRDVSRNNAGEYSCHVENDY 1382

Query: 340  GR 341
            G+
Sbjct: 1383 GQ 1384



 Score = 33.5 bits (73), Expect = 0.003
 Identities = 55/219 (25%), Positives = 80/219 (36%), Gaps = 34/219 (15%)

Query: 172 PPSVRASPETRHVTARRGGAAVLECRAAGNPVPTVTWHRLNDSSSRLGEGPQLQL----- 226
           P  VR  P     +   G  AV+EC A GNP P + W R     + +G+ P L+      
Sbjct: 23  PVFVREPPNRIDFSNTTG--AVVECSAHGNPTPDIIWVR--SDGTAVGDVPGLRQVLANG 78

Query: 227 ------SRLERH----HGGVYACTVDNGVGPPVVTEFQLQVLYPPEITVERSWVHTGEGF 276
                  R E +    H  VY C   N VG     +  ++ +       E    +   G 
Sbjct: 79  NLVFPPFRAEDYRQEVHAQVYVCLAKNSVGSVHSRDVNVRAVVNQYYEAEVVSEYVIRGN 138

Query: 277 RAELVCTV---LADPPAEVSWYQNSFPL-----SASERVTMSARGSNHTLLIADVQPED- 327
            A L C +   +AD     +W  +   L     +   +  +   G  H   I DV PED 
Sbjct: 139 TAVLKCNIPSFVADFVRVEAWVGSDGSLYNHTANYDSKYLVLPSGELH---IRDVGPEDG 195

Query: 328 FGNYSCVADNSLGRARRHVEVSGRPGPARFTSPPLARAP 366
           + +Y C   + L    R   +S   G    T P   +AP
Sbjct: 196 YKSYQCRTKHRLTGETR---LSATKGRLVITEPVGTKAP 231



 Score = 23.0 bits (47), Expect = 4.9
 Identities = 16/45 (35%), Positives = 19/45 (42%)

Query: 425  LRGLQPAAVYEAIVQAKNRYGWNEVSDIFQFHTLGGPHTAHADEL 469
            L  L PA  Y   V A N  G+N     F   T+ G   A A E+
Sbjct: 1551 LLDLDPACWYHLRVTAHNNAGFNVAEYEFATLTVTGGTIAPAREI 1595


>DQ659248-1|ABG47446.1|  496|Tribolium castaneum chitinase 8
           protein.
          Length = 496

 Score = 25.0 bits (52), Expect = 1.2
 Identities = 17/44 (38%), Positives = 21/44 (47%), Gaps = 4/44 (9%)

Query: 408 LAAPAAKGSLTHRVSHELRGLQPAAVYEAIVQAKNRYGWNEVSD 451
           L AP   G    R + E RG+     Y  IV+A+N  GW  V D
Sbjct: 281 LGAPIVAGGDAGRYTGE-RGMMG---YNEIVEAQNAGGWTTVWD 320


>DQ211693-1|ABB16909.1|  314|Tribolium castaneum dorsocross
          protein.
          Length = 314

 Score = 22.6 bits (46), Expect = 6.5
 Identities = 10/28 (35%), Positives = 17/28 (60%), Gaps = 1/28 (3%)

Query: 70 LMVTRDERFRLVDGYNLQITDVGPQIRY 97
          +++T+  R R+    NL+ITD+ P   Y
Sbjct: 39 MIITKSGR-RMFPALNLKITDLDPSADY 65


  Database: tribolium
    Posted date:  Oct 5, 2007 11:13 AM
  Number of letters in database: 114,650
  Number of sequences in database:  317
  
Lambda     K      H
   0.319    0.134    0.412 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 117,730
Number of Sequences: 317
Number of extensions: 4980
Number of successful extensions: 26
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 1
Number of HSP's that attempted gapping in prelim test: 3
Number of HSP's gapped (non-prelim): 14
length of query: 500
length of database: 114,650
effective HSP length: 60
effective length of query: 440
effective length of database: 95,630
effective search space: 42077200
effective search space used: 42077200
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 45 (22.2 bits)