Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001643-TA|BGIBMGA001643-PA|undefined
(404 letters)
Database: rice
37,544 sequences; 14,793,348 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
05_01_0299 - 2322204-2322734,2324089-2326164 36 0.058
12_02_1200 + 26937624-26937674,26937913-26938005,26938582-269387... 34 0.18
03_05_0718 - 27100882-27100929,27101197-27101303,27101446-271015... 33 0.54
09_02_0351 - 7686974-7686983,7687142-7687195,7687363-7687408,768... 32 0.71
01_07_0306 + 42638683-42638990,42639074-42639314,42639935-426402... 32 0.71
04_04_0411 - 25014511-25015015,25015098-25015169,25015700-250157... 32 0.94
02_05_0190 - 26592452-26592693,26592911-26593004,26593425-265936... 32 0.94
06_01_1032 + 8055678-8055782,8055875-8055936,8057302-8057507,805... 31 1.2
10_08_0106 + 14842748-14843085,14843122-14843250,14844145-148442... 31 1.6
10_01_0073 - 955746-955758,955811-956832 31 1.6
10_01_0072 - 918323-918335,918388-919409 31 1.6
04_04_1257 - 32155935-32156526,32156655-32157067 31 2.2
12_02_0046 + 12830974-12832386 30 3.8
08_02_0593 + 19078264-19078609,19078724-19082256 30 3.8
02_02_0331 + 9017291-9019084,9019205-9019402,9020014-9020184,902... 30 3.8
03_01_0540 - 4055635-4055743,4055829-4055981,4056037-4056104,405... 29 5.0
01_01_0224 - 1905978-1907084,1907489-1908070,1908653-1908667 29 6.6
11_06_0717 - 26575009-26575152,26576461-26577978 29 8.8
07_03_1756 - 29239137-29239325,29239741-29240259,29240693-292408... 29 8.8
06_03_0138 + 17093196-17093325,17095704-17095804,17096224-170962... 29 8.8
04_04_1202 - 31700414-31700854 29 8.8
04_04_0827 - 28457858-28458028,28458513-28458669,28458984-284590... 29 8.8
04_01_0611 - 8029101-8029205,8029985-8030107,8030191-8030556,803... 29 8.8
>05_01_0299 - 2322204-2322734,2324089-2326164
Length = 868
Score = 35.9 bits (79), Expect = 0.058
Identities = 23/70 (32%), Positives = 36/70 (51%), Gaps = 4/70 (5%)
Query: 44 TPEPPNSFN--DASNISLPTNNNVPS--VSLSRSIQTRIASDSPNTSTTKTRINQPQSLF 99
TP PPNS+N A + + P N NVPS S+S Q+ I + P ++ + + F
Sbjct: 282 TPVPPNSYNTAKADSSATPANLNVPSSKPSVSGKAQSEIPNLKPKENSNPVPDHSEEQPF 341
Query: 100 IQALTKLNED 109
+ A T+ +D
Sbjct: 342 VAAATQPVKD 351
>12_02_1200 +
26937624-26937674,26937913-26938005,26938582-26938728,
26940299-26940379,26940711-26940849,26940977-26941013,
26941095-26941202,26941434-26941533,26941638-26941783,
26941862-26941953,26942086-26942180,26942435-26942531,
26942623-26942766,26942912-26943129,26943213-26943323
Length = 552
Score = 34.3 bits (75), Expect = 0.18
Identities = 35/129 (27%), Positives = 51/129 (39%), Gaps = 14/129 (10%)
Query: 251 LPYVMRSCKH----HGCALVIASRRGARARTYIRDSGGVMAARVSAEGAGPSYASVLNFK 306
L YVM C H HG +S G + Y+ + G A V+ E N
Sbjct: 71 LGYVMYDCTHYYLHHGQP---SSDPGKHLKKYLHNMAGGSADAVTKEMEALLVGQNPNAV 127
Query: 307 GGE----SNKENIEAHAEEALDAPPRPDIEEEEGFVPVISHGRRPGKNRRDRERKAPPRA 362
GE S+KE A + + +PP D +E +G P G K ++ +K+ +
Sbjct: 128 SGETCETSSKEGKVADSNGSHSSPPEDDDDEAQGDGPSQDGGSEAAKKKK---KKSKSKK 184
Query: 363 HKTPQQHAD 371
K P Q D
Sbjct: 185 KKGPLQQTD 193
>03_05_0718 -
27100882-27100929,27101197-27101303,27101446-27101593,
27102195-27102328,27102608-27102929
Length = 252
Score = 32.7 bits (71), Expect = 0.54
Identities = 22/63 (34%), Positives = 31/63 (49%), Gaps = 1/63 (1%)
Query: 248 CVPLPYVMRSCKHHGCALVIASRRGARARTYIRDSGGVMAARVSAEGAGPSYASVLNFKG 307
C+P+ + K G + + + R G R RD GV+ A VSA AG + AS + G
Sbjct: 37 CLPVLLHVNGSKKQGSSFLSSQRIGLSRRRRRRDLSGVVVAEVSA-AAGVTPASSSSSPG 95
Query: 308 GES 310
G S
Sbjct: 96 GIS 98
>09_02_0351 -
7686974-7686983,7687142-7687195,7687363-7687408,
7688179-7688280,7688683-7689284,7689599-7689783
Length = 332
Score = 32.3 bits (70), Expect = 0.71
Identities = 29/95 (30%), Positives = 43/95 (45%), Gaps = 8/95 (8%)
Query: 252 PYVMRSCKHHGCALVIASRRGARARTYIRDSGGVMAARVSAEGAGPSYASVLNFKGGESN 311
P+++R+ K CALV+AS+ A A D G GAG S + + GE
Sbjct: 88 PFILRN-KKRNCALVVASKPAAEAAG--GDKQGTTKPEQIVPGAGSSSSRTHQPQTGERR 144
Query: 312 KENIEAHAEEALDAPPRPDIEEEEGFVPVI--SHG 344
+ E + ++ A RP EEG + V+ HG
Sbjct: 145 RSTPETGSSKS--AGKRP-ARREEGPLQVVLGKHG 176
>01_07_0306 +
42638683-42638990,42639074-42639314,42639935-42640236,
42640431-42640607,42640853-42641617,42641697-42641791,
42641889-42641951,42642047-42642135,42642229-42642324,
42642457-42643104,42643596-42643628,42643838-42643912,
42644442-42644603,42644604-42644674,42644758-42644815,
42645196-42645394,42645487-42645552,42645699-42646183
Length = 1310
Score = 32.3 bits (70), Expect = 0.71
Identities = 25/79 (31%), Positives = 39/79 (49%), Gaps = 5/79 (6%)
Query: 25 EDNEMASSREIVSHDLNSSTPEPPNSFNDASNISLPTNNNVPSVSLSRS--IQTRIASD- 81
E+ EM S+ H+LN+ST + N SLP +N+ S+ S + +TR + D
Sbjct: 685 EELEMGMSKSSDEHNLNTST-NYSGPLSMGQNSSLPQSNSCVSLEQSEANGPRTRESIDI 743
Query: 82 -SPNTSTTKTRINQPQSLF 99
SPN T ++ + LF
Sbjct: 744 LSPNDENTASQKTHAEELF 762
>04_04_0411 -
25014511-25015015,25015098-25015169,25015700-25015734,
25015805-25015873,25015993-25016044,25016444-25016526,
25016625-25016726,25017150-25017884,25017962-25018012,
25018792-25018983,25019066-25019216,25020012-25020193,
25020329-25020532,25020752-25020859
Length = 846
Score = 31.9 bits (69), Expect = 0.94
Identities = 26/97 (26%), Positives = 44/97 (45%), Gaps = 10/97 (10%)
Query: 279 IRDSGGVMAARVSAEGAGPSYASVLNFKGGESNKENIEAHAEEALDAPPRPDIEEEEGFV 338
+ DSGG R++ + S S GES+K+ + + ++ D PP D +E+
Sbjct: 484 VSDSGGRATRRLAKDDDIKS--SFKKTGEGESSKKKQKENLKQQEDTPPDEDTDEDLSLK 541
Query: 339 PVIS--HGRRPGKNRRD------RERKAPPRAHKTPQ 367
++S + GKN+ +RK A +TPQ
Sbjct: 542 DIVSPKSSAKTGKNKGQAGDSGGSKRKRAQEAEETPQ 578
>02_05_0190 -
26592452-26592693,26592911-26593004,26593425-26593656,
26594660-26595066
Length = 324
Score = 31.9 bits (69), Expect = 0.94
Identities = 26/74 (35%), Positives = 34/74 (45%), Gaps = 6/74 (8%)
Query: 268 ASRRGARARTYIRDSGGVMAARV----SAEGAGPSYASVLNFKGGESNKENIEAHAEEAL 323
+S G R RT R G ++ A SA A + S + GE E + A AEEA+
Sbjct: 43 SSSSGGRPRT--RSGGRLLRATSPPPPSAIAAAACWESRTMRREGEEEWEEVVAGAEEAV 100
Query: 324 DAPPRPDIEEEEGF 337
P P EEEE +
Sbjct: 101 HGAPVPVAEEEEEY 114
>06_01_1032 + 8055678-8055782,8055875-8055936,8057302-8057507,
8057595-8058314,8058404-8058603,8058988-8059172,
8059287-8059354,8059432-8060246,8060502-8060599,
8060702-8060887,8061358-8061538,8061651-8061812,
8061894-8061937,8062059-8062115,8062409-8062505,
8062614-8062786,8062868-8063081,8063270-8063395,
8064072-8064188,8064459-8064566,8064729-8064898,
8065049-8065127,8065211-8065285,8065845-8065942,
8066030-8066137,8066238-8066295,8066527-8066631,
8067461-8069516,8069804-8070697,8070896-8071852,
8072022-8072075,8072157-8072222,8072294-8073472,
8073868-8075598,8075764-8075829,8076763-8077788,
8077893-8078041
Length = 4264
Score = 31.5 bits (68), Expect = 1.2
Identities = 30/123 (24%), Positives = 60/123 (48%), Gaps = 8/123 (6%)
Query: 28 EMASSREIVSHDLNSSTPEPPNSFNDASNISLPTNNNVPSVSLSRSIQTRIASDSPNTST 87
E+ +S E + ++ P PP +DA+++S + +P+ ++ + SP+ +
Sbjct: 4129 EIGTSPECDDDQVATAAPHPPTPLSDATDVS--ADVQIPAGISEAKLEQPNETTSPSGAA 4186
Query: 88 TKTRINQPQSLFIQALTKLNEDSVKFTVINIIKTQQEMAKMA-LNDDQHTLSAPDSLMSA 146
T+ N S I L + + + T I T+ + A+ A ++DD++ ++A D SA
Sbjct: 4187 TEEN-NTVVSTQIPTLAESEDRTPADTAIQ--GTEVDSAEPASVSDDENKVTAADD--SA 4241
Query: 147 PPS 149
PS
Sbjct: 4242 LPS 4244
Score = 28.7 bits (61), Expect = 8.8
Identities = 16/54 (29%), Positives = 28/54 (51%)
Query: 270 RRGARARTYIRDSGGVMAARVSAEGAGPSYASVLNFKGGESNKENIEAHAEEAL 323
RRG + ++ +G V+ + V A G+G +YAS + + N + A+A L
Sbjct: 1748 RRGRKPKSLAASAGDVILSPVVAVGSGEAYASSVVSSYPQGNVSSSHANAMAGL 1801
>10_08_0106 +
14842748-14843085,14843122-14843250,14844145-14844211,
14847177-14847324,14847998-14848097,14848306-14848384,
14848527-14848687,14848829-14848908,14849319-14849475,
14849575-14849723,14849909-14850076,14850426-14850719,
14851002-14851046,14851213-14851462,14851707-14851835,
14852799-14853039,14853977-14854642
Length = 1066
Score = 31.1 bits (67), Expect = 1.6
Identities = 22/97 (22%), Positives = 40/97 (41%), Gaps = 4/97 (4%)
Query: 268 ASRRGARARTYIRDSGGVMAARVSAEGAGPSYASVLNFKGGESNKENIEAHAEEALDAPP 327
ASR A + +++ AR A+G S S ++F GG+ N + + + D PP
Sbjct: 900 ASRHEAAEHAHAKNTAAAADARSKAKG---SVFSRISFPGGDGNPSDAKRSRRSSSDKPP 956
Query: 328 RPDIEEEEGFVPVISHGRRPGKNRRDRERKAPPRAHK 364
+ ++ G++ R R+A A +
Sbjct: 957 ASSSSSSKKARAAVAED-NDGRHHRRHHREAAAAAEE 992
>10_01_0073 - 955746-955758,955811-956832
Length = 344
Score = 31.1 bits (67), Expect = 1.6
Identities = 14/40 (35%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Query: 323 LDAPPRPDIEEEEGFVPVISHGRRPGKNRRDRERKAPPRA 362
+D PP P + ++G P + ++P K+ RE APP A
Sbjct: 184 IDEPPPPK-KRQQGRQPKVPRAKKPKKSAAPREDGAPPNA 222
>10_01_0072 - 918323-918335,918388-919409
Length = 344
Score = 31.1 bits (67), Expect = 1.6
Identities = 14/40 (35%), Positives = 22/40 (55%), Gaps = 1/40 (2%)
Query: 323 LDAPPRPDIEEEEGFVPVISHGRRPGKNRRDRERKAPPRA 362
+D PP P + ++G P + ++P K+ RE APP A
Sbjct: 184 IDEPPPPK-KRQQGRQPKVPRAKKPKKSAAPREDGAPPNA 222
>04_04_1257 - 32155935-32156526,32156655-32157067
Length = 334
Score = 30.7 bits (66), Expect = 2.2
Identities = 22/95 (23%), Positives = 40/95 (42%), Gaps = 2/95 (2%)
Query: 4 KDLNVSTQFGSNCEQVENRIGEDNEMASSREIVSHDLNSST--PEPPNSFNDASNISLPT 61
KD +S E + ++ SS + ++++ P PPNS + SN T
Sbjct: 217 KDCQLSVLLTKTAEIQRTTLRLSRQLVSSNSNIDDIIHNTNAPPAPPNSAINHSNNIFST 276
Query: 62 NNNVPSVSLSRSIQTRIASDSPNTSTTKTRINQPQ 96
N+++ R + + SP + + + NQPQ
Sbjct: 277 NHSLHRQPQQRVVVDGRSCVSPRPTPGQAKGNQPQ 311
>12_02_0046 + 12830974-12832386
Length = 470
Score = 29.9 bits (64), Expect = 3.8
Identities = 20/65 (30%), Positives = 25/65 (38%), Gaps = 1/65 (1%)
Query: 145 SAPPSYSFVLRQIAARPRTRFMGTFIPSPSFIQHTPPPNYASAFDVYVDNPMPRPSRIYN 204
SAPP + A P R M P P TPP +A D + P P P I +
Sbjct: 308 SAPPPWCVPPPPRPAPPSRRVMAPLPPYPMDPFETPPSPHAPRHDPFEPPPSPDPP-IQS 366
Query: 205 FGFSP 209
+ P
Sbjct: 367 YAIDP 371
>08_02_0593 + 19078264-19078609,19078724-19082256
Length = 1292
Score = 29.9 bits (64), Expect = 3.8
Identities = 22/79 (27%), Positives = 30/79 (37%)
Query: 283 GGVMAARVSAEGAGPSYASVLNFKGGESNKENIEAHAEEALDAPPRPDIEEEEGFVPVIS 342
GG A R A G G + +V+ S E + A AE+ + P +E E +
Sbjct: 77 GGSGARREDAAGTGRNAVAVVAGGSATSGAERVAARAEDGRRSMDGPTLELSEMMLHAAQ 136
Query: 343 HGRRPGKNRRDRERKAPPR 361
R R R PPR
Sbjct: 137 PWRSRCTQRDVRPGAVPPR 155
>02_02_0331 +
9017291-9019084,9019205-9019402,9020014-9020184,
9020292-9020435,9020552-9020764,9020859-9021929,
9022365-9022391
Length = 1205
Score = 29.9 bits (64), Expect = 3.8
Identities = 14/48 (29%), Positives = 19/48 (39%)
Query: 170 IPSPSFIQHTPPPNYASAFDVYVDNPMPRPSRIYNFGFSPMFVSCPEC 217
IP P H P P + +A+ Y P P P +G+ C C
Sbjct: 47 IPPPHLYYHGPFPQHPNAYPSYFARPPPYPVDQTAYGYDKFKSHCCGC 94
>03_01_0540 -
4055635-4055743,4055829-4055981,4056037-4056104,
4056486-4056580,4057180-4057241,4057371-4057410,
4058124-4059141,4059231-4059611,4059873-4059880,
4061005-4061067,4061416-4061719
Length = 766
Score = 29.5 bits (63), Expect = 5.0
Identities = 25/93 (26%), Positives = 37/93 (39%), Gaps = 4/93 (4%)
Query: 151 SFVLRQIAARPRTRFMGTFIPSPSFIQHTPPPNYASAFDVYVDNPMPRPSRIYNFGFSPM 210
S VL ++ PR R GT++ ++ +T P D+ D P P FG P
Sbjct: 261 SLVLFRVNLSPRPRRSGTYMSPCNYFVYTAGPG-RPWLDLLPD-PNAMPFNSQQFGLFPC 318
Query: 211 FVSCPECGYTGMSIAISQITMSTHMCAFILCLF 243
E + ++ S+ S C F LC F
Sbjct: 319 RSGVSE--HYDVAFLNSEWVASDEACQFELCTF 349
>01_01_0224 - 1905978-1907084,1907489-1908070,1908653-1908667
Length = 567
Score = 29.1 bits (62), Expect = 6.6
Identities = 13/25 (52%), Positives = 15/25 (60%)
Query: 179 TPPPNYASAFDVYVDNPMPRPSRIY 203
TPPP+ SA + D P PRPS Y
Sbjct: 47 TPPPHPLSATEDDADEPRPRPSGRY 71
>11_06_0717 - 26575009-26575152,26576461-26577978
Length = 553
Score = 28.7 bits (61), Expect = 8.8
Identities = 18/68 (26%), Positives = 31/68 (45%), Gaps = 1/68 (1%)
Query: 282 SGGVMAARVSAEGAGPSYASVLNFK-GGESNKENIEAHAEEALDAPPRPDIEEEEGFVPV 340
+G ++ +G + A V K GG++ HA LD+ P D + GF+ +
Sbjct: 45 AGATRLMEMTTKGRADANAKVNQLKAGGDTKFLPALKHASGLLDSRPAGDKQYRPGFIFL 104
Query: 341 ISHGRRPG 348
+S G+ G
Sbjct: 105 LSDGQDNG 112
>07_03_1756 -
29239137-29239325,29239741-29240259,29240693-29240800,
29240856-29241155,29241730-29241831,29243042-29243209,
29243878-29243991,29244184-29244288,29244372-29244502,
29244794-29244918,29245001-29245172,29245277-29245316,
29245636-29245722,29245803-29245868,29246412-29246510,
29246714-29246836,29247066-29247107,29247195-29247260,
29247362-29247553,29247672-29247737,29248839-29249141
Length = 1038
Score = 28.7 bits (61), Expect = 8.8
Identities = 19/63 (30%), Positives = 27/63 (42%), Gaps = 2/63 (3%)
Query: 279 IRDSGGVMAARVSAEGAGPSYASVLNFKGGESNKENIEAHAEEALDAPPRPDIEEEEGFV 338
+R G + RVS G + S N G +S EA AE+A+ A D + V
Sbjct: 33 LRVLGALRGGRVSTLGRRARFCS--NSAGSDSEAAAAEAKAEDAVAAEGEADGKASSAIV 90
Query: 339 PVI 341
P +
Sbjct: 91 PTV 93
>06_03_0138 +
17093196-17093325,17095704-17095804,17096224-17096292,
17096403-17097650,17097763-17097871,17099459-17099655,
17099732-17099842,17099927-17100079
Length = 705
Score = 28.7 bits (61), Expect = 8.8
Identities = 35/170 (20%), Positives = 68/170 (40%), Gaps = 18/170 (10%)
Query: 57 ISLPTNNNVPSVSLSRSIQTRIASDSPNTSTTKTRINQPQSLFIQALTKLNEDSVKFTVI 116
+SLP ++ VPS S ++ + + + P++ I + + +T + + T+
Sbjct: 356 VSLPLDSTVPSASSNKPMNIPVPTYLPSSQPPLANIAASAATVAEPVTLVTPGQLLPTIS 415
Query: 117 NIIKTQQ--EMAKMALNDDQHTLSAPDSLMSAPPSYSFVLRQIAARPRTRFMGTFIPSPS 174
+ + + E A + + S S +A + S V + + +F + +PS
Sbjct: 416 STVLSADALETASAMIPSPKAASSMVSSSQAALAASSQVTSSVVSPSEDQFASSPVPSHQ 475
Query: 175 FIQ------HTPPPNY-ASAFDVYVDN-----PMPRP----SRIYNFGFS 208
++ HT P + A V N P P+P S +FGFS
Sbjct: 476 QLEQNNEDRHTKKPEWKAKQHSVAPSNKEPLLPAPKPTLQKSHFQSFGFS 525
>04_04_1202 - 31700414-31700854
Length = 146
Score = 28.7 bits (61), Expect = 8.8
Identities = 16/46 (34%), Positives = 23/46 (50%)
Query: 286 MAARVSAEGAGPSYASVLNFKGGESNKENIEAHAEEALDAPPRPDI 331
+ AR S E A P +S++ + ++ AEE DAPP P I
Sbjct: 6 LVARFSVEVAPPLLSSIIRPRRRRGFPAMLDTIAEEEPDAPPLPAI 51
>04_04_0827 -
28457858-28458028,28458513-28458669,28458984-28459083,
28459204-28459231,28459321-28459396,28461276-28461355,
28461642-28461805,28461897-28462056,28462154-28462277,
28462901-28463941,28464141-28464259,28464512-28464823,
28465222-28465425,28466068-28466751,28467301-28467474
Length = 1197
Score = 28.7 bits (61), Expect = 8.8
Identities = 15/41 (36%), Positives = 19/41 (46%), Gaps = 2/41 (4%)
Query: 28 EMASSREIVSHDLNSSTPEPPNSFND--ASNISLPTNNNVP 66
E S +V S +P PN ND + NI P N N+P
Sbjct: 661 EQVSHANVVCRHSESGSPRLPNRDNDEKSINIKQPWNTNIP 701
>04_01_0611 -
8029101-8029205,8029985-8030107,8030191-8030556,
8030639-8031124
Length = 359
Score = 28.7 bits (61), Expect = 8.8
Identities = 24/73 (32%), Positives = 34/73 (46%), Gaps = 17/73 (23%)
Query: 279 IRDSGGVMAARVSAEGAGPSY-----------ASVLNFKGGESNKE-NIEAHAEEAL--- 323
+R+ G A RV A+GA PSY A+ + +GG + K +E EE
Sbjct: 34 VREEGHSYAVRVPADGANPSYYLQYEKPLTNGAASTSSRGGAARKRCRMEEEEEEESSSG 93
Query: 324 --DAPPRPDIEEE 334
+ P PDIEE+
Sbjct: 94 EPEPAPDPDIEED 106
Database: rice
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 14,793,348
Number of sequences in database: 37,544
Lambda K H
0.317 0.131 0.392
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 12,586,068
Number of Sequences: 37544
Number of extensions: 579081
Number of successful extensions: 1821
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 11
Number of HSP's successfully gapped in prelim test: 12
Number of HSP's that attempted gapping in prelim test: 1805
Number of HSP's gapped (non-prelim): 31
length of query: 404
length of database: 14,793,348
effective HSP length: 84
effective length of query: 320
effective length of database: 11,639,652
effective search space: 3724688640
effective search space used: 3724688640
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 61 (28.7 bits)
- SilkBase 1999-2023 -