Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001642-TA|BGIBMGA001642-PA|IPR001930|Peptidase M1,
membrane alanine aminopeptidase
         (544 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At4g33090.1 68417.m04715 aminopeptidase M similar to SP|Q11011 P...    54   3e-07
At1g61390.1 68414.m06918 S-locus protein kinase, putative contai...    29   7.9  

>At4g33090.1 68417.m04715 aminopeptidase M similar to SP|Q11011
           Puromycin-sensitive aminopeptidase (EC 3.4.11.-) (PSA)
           {Mus musculus}; contains Pfam profile PF01433: Peptidase
           family M1
          Length = 879

 Score = 53.6 bits (123), Expect = 3e-07
 Identities = 28/99 (28%), Positives = 47/99 (47%), Gaps = 3/99 (3%)

Query: 23  ADIESATDELSLHKGAAIVSMAIEAAGEETGXXXXXXXXXDHRYASADARDLWRALQRDN 82
           A+I+   D +S  KGA+++ M     G E           +H Y++A   DLW AL+  +
Sbjct: 381 AEIDEIFDAISYRKGASVIRMLQSYLGAEVFQKSLAAYIKNHAYSNAKTEDLWAALEAGS 440

Query: 83  PDDASVQAHAWDGWCEKPGYPLLSATTVGDDVILKQERF 121
            +  +        W ++ GYP++SA      + L+Q RF
Sbjct: 441 GEPVN---KLMSSWTKQKGYPVVSAKIKDGKLELEQSRF 476



 Score = 34.7 bits (76), Expect = 0.16
 Identities = 53/245 (21%), Positives = 90/245 (36%), Gaps = 17/245 (6%)

Query: 293 GKHKWIRYNVGARGLYRVAPQDRAGEEASDAARVYDGASAAERALILDDAFVLSRAGRLP 352
           G   WI+ NV   G YRV   D       +A       ++ +R  ILDD+F L+ A +  
Sbjct: 532 GTCSWIKINVDQAGFYRVKYDDSLAAGLRNATE-SQSLTSIDRYGILDDSFALTMARQQS 590

Query: 353 ASXXXXXXXXXXGEQHWAPWRVVLSHMSWWXXXXXXXXXXXXXXXXXXXXHPPIALRH-- 410
            +           E  +     VLS++                       H  I +    
Sbjct: 591 LASLLTLCSAYKKELDY----TVLSNLIAISYKVVKIGADANQELMSGIKHFFIGVFQFA 646

Query: 411 -------QRDADSDDHLWLRGALLASGVEWGNQGITNEAVQLFDLWMEKNHT--IPEIYQ 461
                   +  +S     LRG +L +   +G+     EAV+ FD ++   +T  +P   +
Sbjct: 647 AGKLGWDPKQGESHLDAMLRGEVLTALAVFGHDETLKEAVRRFDAFLADRNTPLLPPDIR 706

Query: 462 EAAFTAGVRTHGRVAWRACWRALVDSYSAPRPTYSHRALLAALASPEDDWLFYRFAFTVL 521
            AA+ A V      + ++ + +L+  Y     +     +L +LAS  D  +       VL
Sbjct: 707 RAAYVA-VMQRANKSDKSGYESLLRVYRETDLSQEKTRILGSLASCPDPTIVQDVLNFVL 765

Query: 522 STEAQ 526
           S E +
Sbjct: 766 SDEVR 770


>At1g61390.1 68414.m06918 S-locus protein kinase, putative contains
           protein kinase domain, Pfam:PF00069; contains S-locus
           glycoprotein family domain, Pfam:PF00954
          Length = 831

 Score = 29.1 bits (62), Expect = 7.9
 Identities = 10/28 (35%), Positives = 17/28 (60%)

Query: 78  LQRDNPDDASVQAHAWDGWCEKPGYPLL 105
           + R + +  ++ A+AW+ WCE  G  LL
Sbjct: 721 ISRFSEEGKTLLAYAWESWCETKGVDLL 748


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.320    0.133    0.433 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 11,040,206
Number of Sequences: 28952
Number of extensions: 399850
Number of successful extensions: 733
Number of sequences better than 10.0: 2
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 728
Number of HSP's gapped (non-prelim): 4
length of query: 544
length of database: 12,070,560
effective HSP length: 85
effective length of query: 459
effective length of database: 9,609,640
effective search space: 4410824760
effective search space used: 4410824760
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 62 (29.1 bits)