Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001638-TA|BGIBMGA001638-PA|IPR000994|Peptidase M24,
catalytic core
(630 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g36760.1 68417.m05216 aminopeptidase P similar to Xaa-Pro ami... 324 1e-88
At3g05350.1 68416.m00583 aminopeptidase P, cytosolic, putative s... 305 4e-83
At4g36760.2 68417.m05215 aminopeptidase P similar to Xaa-Pro ami... 287 1e-77
At1g09300.1 68414.m01041 metallopeptidase M24 family protein sim... 38 0.015
At4g29490.1 68417.m04208 Xaa-Pro dipeptidase, putative / prolida... 37 0.047
At5g17780.1 68418.m02085 hydrolase, alpha/beta fold family prote... 30 5.3
At3g22190.1 68416.m02800 calmodulin-binding family protein conta... 29 9.3
At1g74030.1 68414.m08573 enolase, putative similar to Swiss-Prot... 29 9.3
>At4g36760.1 68417.m05216 aminopeptidase P similar to Xaa-Pro
aminopeptidase 2 [Lycopersicon esculentum] GI:15384991;
contains Pfam profile PF00557: metallopeptidase family
M24
Length = 634
Score = 324 bits (795), Expect = 1e-88
Identities = 207/622 (33%), Positives = 331/622 (53%), Gaps = 54/622 (8%)
Query: 13 MSSQRPVLAAYIIPTADAHNSEYIDAADARREWISAFTGSAGTAVVTSSQALVWTDGRYY 72
M+S P L A ++P+ D H SEY+ A D RRE++S F+GSAG A++T +A +WTDGRY+
Sbjct: 1 MASHSPPLDALVVPSEDYHQSEYVSARDKRREFVSGFSGSAGLALITKKEARLWTDGRYF 60
Query: 73 TQFEREVDLSAWTLMKQTLPDTPTLEKWLASNLKDGDVVGVDPQTMTRDEWTPIQTALKK 132
Q +++ WTLM+ + + P +E W++ NL + +GVD ++ D + K
Sbjct: 61 LQALQQLS-DEWTLMR--MGEDPLVEVWMSDNLPEEANIGVDSWCVSVDTANRWGKSFAK 117
Query: 133 ISAQLVPISNNLVDDVRMELGDPAPKRSHNELAPLHVRYTGRTAGEKIAELRRKMAEKKA 192
+ +L+ + +LVD+V P + S + PL + GR+ K +LR K+ ++ A
Sbjct: 118 KNQKLITTTTDLVDEVWKSR--PPSEMSPVVVHPLE--FAGRSVSHKFEDLRAKLKQEGA 173
Query: 193 SALVLTALDDIAYTLNLRGSDIEYNPVFFSYLIVMETNVVLYWGDGQLPPSVTDHLREEG 252
LV+ ALD++A+ N+RG+D+ Y PV ++ I+ + LY ++ + G
Sbjct: 174 RGLVIAALDEVAWLYNIRGTDVAYCPVVHAFAILTTDSAFLYVDKKKVSDEANSYFNGLG 233
Query: 253 VDSVEGKPYGDILEGLKEMARE-LSESGDGRHV-------IWLSNDANEAIH-RAASGSD 303
V E + Y D++ + +A + L S + V + + +D + + AS
Sbjct: 234 V---EVREYTDVISDVALLASDRLISSFASKTVQHEAAKDMEIDSDQPDRLWVDPASCCY 290
Query: 304 VLKRPID---LISEVSPVKLAKLVKNEIELQGFRNCHIKDGIAVVRFFRWLHDQID---- 356
L +D ++ + SP+ L+K +KN +EL+G +N H++DG AVV++ WL +Q+
Sbjct: 291 ALYSKLDAEKVLLQPSPISLSKALKNPVELEGIKNAHVRDGAAVVQYLVWLDNQMQELYG 350
Query: 357 -SG---------------EQITEVQASDKLLEFRRDEKDFMGPSFGTIAGSGANGAIIHY 400
SG ++TEV SDKL R ++ F G SF TI+ G+N A+IHY
Sbjct: 351 ASGYFLEAEASKKKPSETSKLTEVTVSDKLESLRASKEHFRGLSFPTISSVGSNAAVIHY 410
Query: 401 SPDREGQQTVIERDHMFLLDSGGQYRDGTTDITRTRHMSGNPTAEQKDAFTRVLKGQIMI 460
SP+ E + + D ++L DSG QY DGTTDITRT H G P+A +K+ +T V KG + +
Sbjct: 411 SPEPEACAEM-DPDKIYLCDSGAQYLDGTTDITRTVHF-GKPSAHEKECYTAVFKGHVAL 468
Query: 461 GSALFPKGVKGNVLDSFARHALWEXXXXXXXXXXXXXXXXXNVHEGPSGVSWRPYPDDPG 520
G+A FPKG G LD AR LW+ VHEGP VS+RP +
Sbjct: 469 GNARFPKGTNGYTLDILARAPLWKYGLDYRHGTGHGVGSYLCVHEGPHQVSFRPSARNVP 528
Query: 521 LNVGQILSNEPGFYKVGEYGIRHEDLIEIVAVDKGSDHPKAKNLRGDYDGRGVLGFSTLT 580
L +++EPG+Y+ G +GIR E+++ + +D N GD +G L F +T
Sbjct: 529 LQATMTVTDEPGYYEDGNFGIRLENVLVV------NDAETEFNF-GD---KGYLQFEHIT 578
Query: 581 LVPNQRECIDAEIMTDFEMSYV 602
P Q + ID + +T E+ ++
Sbjct: 579 WAPYQVKLIDLDELTREEIDWL 600
>At3g05350.1 68416.m00583 aminopeptidase P, cytosolic, putative
similar to cytosolic aminopeptidase P from [Homo
sapiens] GI:8489879, [Rattus norvegicus] GI:2760920;
contains Pfam profile PF00557: metallopeptidase family
M24
Length = 569
Score = 305 bits (750), Expect = 4e-83
Identities = 190/555 (34%), Positives = 289/555 (52%), Gaps = 59/555 (10%)
Query: 87 MKQTLPDTPTLEKWLASNLKDGDVVGVDPQTMTRDEWTPIQTALKKISAQLVPISN-NLV 145
M+ P PT +W+A L G VG+DP + D ++ + K + +LV + N NLV
Sbjct: 1 MRAGNPGVPTASEWIADVLAPGGRVGIDPFLFSADAAEELKEVIAKKNHELVYLYNVNLV 60
Query: 146 DDVRMELGDPAPKRSHNELAPLHVRYTGRTAGEKIAELRRKMAEKKASALVLTALDDIAY 205
D++ D PK ++ ++Y G K+ LR ++ + SA+V++ LD+IA+
Sbjct: 61 DEIWK---DSRPKPPSRQIRIHDLKYAGLDVASKLLSLRNQIMDAGTSAIVISMLDEIAW 117
Query: 206 TLNLRGSDIEYNPVFFSYLIVMETNVVLYWGDGQLPPSVTDHLREEGVDSVEGKPYGDIL 265
LNLRGSD+ ++PV ++YLIV L+ + ++ V DHL+ G+ E +PY IL
Sbjct: 118 VLNLRGSDVPHSPVMYAYLIVEVDQAQLFVDNSKVTVEVKDHLKNAGI---ELRPYDSIL 174
Query: 266 EGLKEMARELSE-----------------SGDGRHVIWLSNDANEAIHRAASGSDVLKRP 308
+G+ +A ++ S R+ ++A S S P
Sbjct: 175 QGIDSLAARGAQLLMDPSTLNVAIISTYKSACERYSRNFESEAKVKTKFTDSSSGYTANP 234
Query: 309 IDLISEVSPVKLAKLVKNEIELQGFRNCHIKDGIAVVRFFRWLHDQIDSGEQITEVQASD 368
+ + SP+ AK +KN+ EL+G +N H++D A+ F+ WL +++ +TEV +D
Sbjct: 235 SGIYMQ-SPISWAKAIKNDAELKGMKNSHLRDAAALAHFWAWLEEEVHKNANLTEVDVAD 293
Query: 369 KLLEFRRDEKDFMGPSFGTIAG---------------------SGANGAIIHYSPDREGQ 407
+LLEFR + FM SF TI+G SGANGAIIHY P+ E
Sbjct: 294 RLLEFRSMQDGFMDTSFDTISGMVIVTLVGGTCFLTHSQYTASSGANGAIIHYKPEPESC 353
Query: 408 QTVIERDHMFLLDSGGQYRDGTTDITRTRHMSGNPTAEQKDAFTRVLKGQIMIGSALFPK 467
V + +FLLDSG QY DGTTDITRT H S P+A +K+ FTRVL+G I + A+FP+
Sbjct: 354 SRV-DPQKLFLLDSGAQYVDGTTDITRTVHFS-EPSAREKECFTRVLQGHIALDQAVFPE 411
Query: 468 GVKGNVLDSFARHALWEXXXXXXXXXXXXXXXXXNVHEGPSGVSWRPYPDDPGLNVGQIL 527
G G VLD FAR +LW+ NVHEGP +S+R Y + L G I+
Sbjct: 412 GTPGFVLDGFARSSLWKIGLDYRHGTGHGVGAALNVHEGPQSISFR-YGNMTPLQNGMIV 470
Query: 528 SNEPGFYKVGEYGIRHEDLIEIVAVDKGSDHPKAKNLRGDYDGRGVLGFSTLTLVPNQRE 587
SNEPG+Y+ +GIR E+L+ + + ++ P + G LGF LT P Q +
Sbjct: 471 SNEPGYYEDHAFGIRIENLLHV----RDAETPNR------FGGATYLGFEKLTFFPIQTK 520
Query: 588 CIDAEIMTDFEMSYV 602
+D +++D E+ ++
Sbjct: 521 MVDVSLLSDTEVDWL 535
>At4g36760.2 68417.m05215 aminopeptidase P similar to Xaa-Pro
aminopeptidase 2 [Lycopersicon esculentum] GI:15384991;
contains Pfam profile PF00557: metallopeptidase family
M24
Length = 519
Score = 287 bits (705), Expect = 1e-77
Identities = 179/531 (33%), Positives = 283/531 (53%), Gaps = 44/531 (8%)
Query: 13 MSSQRPVLAAYIIPTADAHNSEYIDAADARREWISAFTGSAGTAVVTSSQALVWTDGRYY 72
M+S P L A ++P+ D H SEY+ A D RRE++S F+GSAG A++T +A +WTDGRY+
Sbjct: 1 MASHSPPLDALVVPSEDYHQSEYVSARDKRREFVSGFSGSAGLALITKKEARLWTDGRYF 60
Query: 73 TQFEREVDLSAWTLMKQTLPDTPTLEKWLASNLKDGDVVGVDPQTMTRDEWTPIQTALKK 132
Q +++ WTLM+ + + P +E W++ NL + +GVD ++ D + K
Sbjct: 61 LQALQQLS-DEWTLMR--MGEDPLVEVWMSDNLPEEANIGVDSWCVSVDTANRWGKSFAK 117
Query: 133 ISAQLVPISNNLVDDVRMELGDPAPKRSHNELAPLHVRYTGRTAGEKIAELRRKMAEKKA 192
+ +L+ + +LVD+V P + S + PL + GR+ K +LR K+ ++ A
Sbjct: 118 KNQKLITTTTDLVDEVWK--SRPPSEMSPVVVHPL--EFAGRSVSHKFEDLRAKLKQEGA 173
Query: 193 SALVLTALDDIAYTLNLRGSDIEYNPVFFSYLIVMETNVVLYWGDGQLPPSVTDHLREEG 252
LV+ ALD++A+ N+RG+D+ Y PV ++ I+ + LY ++ + G
Sbjct: 174 RGLVIAALDEVAWLYNIRGTDVAYCPVVHAFAILTTDSAFLYVDKKKVSDEANSYFNGLG 233
Query: 253 VDSVEGKPYGDILEGLKEMARE-LSESGDGRHV-------IWLSNDANEAIH-RAASGSD 303
VE + Y D++ + +A + L S + V + + +D + + AS
Sbjct: 234 ---VEVREYTDVISDVALLASDRLISSFASKTVQHEAAKDMEIDSDQPDRLWVDPASCCY 290
Query: 304 VLKRPID---LISEVSPVKLAKLVKNEIELQGFRNCHIKDGIAVVRFFRWLHDQID---- 356
L +D ++ + SP+ L+K +KN +EL+G +N H++DG AVV++ WL +Q+
Sbjct: 291 ALYSKLDAEKVLLQPSPISLSKALKNPVELEGIKNAHVRDGAAVVQYLVWLDNQMQELYG 350
Query: 357 -SG---------------EQITEVQASDKLLEFRRDEKDFMGPSFGTIAGSGANGAIIHY 400
SG ++TEV SDKL R ++ F G SF TI+ G+N A+IHY
Sbjct: 351 ASGYFLEAEASKKKPSETSKLTEVTVSDKLESLRASKEHFRGLSFPTISSVGSNAAVIHY 410
Query: 401 SPDREGQQTVIERDHMFLLDSGGQYRDGTTDITRTRHMSGNPTAEQKDAFTRVLKGQIMI 460
SP+ E ++ D ++L DSG QY DGTTDITRT H G P+A +K+ +T V KG + +
Sbjct: 411 SPEPEA-CAEMDPDKIYLCDSGAQYLDGTTDITRTVHF-GKPSAHEKECYTAVFKGHVAL 468
Query: 461 GSALFPKGVKGNVLDSFARHALWEXXXXXXXXXXXXXXXXXNVHEGPSGVS 511
G+A FPKG G LD AR LW+ VHEG + S
Sbjct: 469 GNARFPKGTNGYTLDILARAPLWKYGLDYRHGTGHGVGSYLCVHEGKTSPS 519
>At1g09300.1 68414.m01041 metallopeptidase M24 family protein
similar to SP|P15034 Xaa-Pro aminopeptidase (EC
3.4.11.9) (X-Pro aminopeptidase) (Aminopeptidase P II)
(Aminoacylproline aminopeptidase) {Escherichia coli};
contains Pfam profiles PF00557: metallopeptidase family
M24, PF05195: Aminopeptidase P, N-terminal domain
Length = 493
Score = 38.3 bits (85), Expect = 0.015
Identities = 21/72 (29%), Positives = 40/72 (55%), Gaps = 4/72 (5%)
Query: 384 SFGTIAGSGANGAIIHYSPDREGQQTVIERDHMFLLDSGGQYRDGTTDITRTRHMSGNPT 443
+F + G G+N ++IHYS R Q+ I+ + L+D G + +D+TRT G +
Sbjct: 264 AFNPVVGGGSNASVIHYS--RNDQR--IKDGDLVLMDMGCELHGYVSDLTRTWPPCGKFS 319
Query: 444 AEQKDAFTRVLK 455
+ Q++ + +L+
Sbjct: 320 SVQEELYDLILQ 331
>At4g29490.1 68417.m04208 Xaa-Pro dipeptidase, putative / prolidase,
putative / imidodipeptidase, putative similar to
SP|P12955 Xaa-Pro dipeptidase (EC 3.4.13.9) (X-Pro
dipeptidase) (Proline dipeptidase) (Prolidase)
(Imidodipeptidase) {Homo sapiens}; contains Pfam
profiles PF00557: metallopeptidase family M24, PF05195:
Aminopeptidase P, N-terminal domain
Length = 333
Score = 36.7 bits (81), Expect = 0.047
Identities = 19/63 (30%), Positives = 31/63 (49%)
Query: 384 SFGTIAGSGANGAIIHYSPDREGQQTVIERDHMFLLDSGGQYRDGTTDITRTRHMSGNPT 443
S+ I +G N A++HY E + LLD G +Y +DIT + ++G T
Sbjct: 238 SYTCICATGDNSAVLHYGHAAAPNDRTFEDGDLALLDMGAEYHFYGSDITCSFPVNGKFT 297
Query: 444 AEQ 446
++Q
Sbjct: 298 SDQ 300
>At5g17780.1 68418.m02085 hydrolase, alpha/beta fold family protein
low similarity to SP|Q02104 Lipase 1 precursor (EC
3.1.1.3) (Triacylglycerol lipase) {Psychrobacter
immobilis}, SP|P27747|ACOC_ALCEU Dihydrolipoamide
acetyltransferase component of acetoin cleaving system
(EC 2.3.1.12) [Ralstonia eutropha] {Alcaligenes
eutrophus}; contains Pfam profile PF00561: hydrolase,
alpha/beta fold family
Length = 417
Score = 29.9 bits (64), Expect = 5.3
Identities = 11/33 (33%), Positives = 19/33 (57%)
Query: 451 TRVLKGQIMIGSALFPKGVKGNVLDSFARHALW 483
+ ++K ++ FP V+G+VL+ AR LW
Sbjct: 239 SNIVKSVTLVAPPYFPSSVEGSVLNRIARKRLW 271
>At3g22190.1 68416.m02800 calmodulin-binding family protein contains
Pfam profile PF00612: IQ calmodulin-binding motif
Length = 400
Score = 29.1 bits (62), Expect = 9.3
Identities = 21/84 (25%), Positives = 43/84 (51%), Gaps = 5/84 (5%)
Query: 72 YTQFEREVDLSAWTLMKQTLPDTPTLEKWLASNLKDGDVVGVDPQTMTRDEWTPIQTALK 131
++ F+ + + W +++ + P ++L SNL+D +G M + E P +T +K
Sbjct: 229 HSGFQPDKNNWGWNWLERWMAVRPWENRFLDSNLRDDAKLG--ENGMEQSENVP-KTQIK 285
Query: 132 KISAQLVPISNNLVDDVRMELGDP 155
+S +P ++NLV V ++ P
Sbjct: 286 SVSK--MPNTSNLVSGVSSQMTGP 307
>At1g74030.1 68414.m08573 enolase, putative similar to
Swiss-Prot:P15007 enolase (EC 4.2.1.11)
(2-phosphoglycerate dehydratase)(2-phospho-D- glycerate
hydro-lyase) [Drosophila melanogaster]
Length = 477
Score = 29.1 bits (62), Expect = 9.3
Identities = 31/142 (21%), Positives = 57/142 (40%), Gaps = 10/142 (7%)
Query: 47 SAFTGSAGTAVVTSSQALVWTDGRYYTQFEREVDLSAWTLMKQTLPDTPTLEKWLASNLK 106
+ +TG + ++ DGRY F+++ + A L ++L D +K
Sbjct: 279 AGYTGKIKIGMDVAASEFFMKDGRYDLNFKKQPNDGAHVLSAESLADL------YREFIK 332
Query: 107 DGDVVGVDPQTMTRDEWTPIQTALKKISAQLVPISNNLVDDVRMELGDPAPKRSHNELAP 166
D +V ++ +D+W+ + + QLV ++L+ + + K+S N L
Sbjct: 333 DFPIVSIE-DPFDQDDWSSWASLQSSVDIQLV--GDDLLVTNPKRIAEAIKKQSCNALL- 388
Query: 167 LHVRYTGRTAGEKIAELRRKMA 188
L V G A L K A
Sbjct: 389 LKVNQIGTVTESIQAALDSKAA 410
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.317 0.135 0.402
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 14,634,212
Number of Sequences: 28952
Number of extensions: 633268
Number of successful extensions: 1477
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 1450
Number of HSP's gapped (non-prelim): 15
length of query: 630
length of database: 12,070,560
effective HSP length: 85
effective length of query: 545
effective length of database: 9,609,640
effective search space: 5237253800
effective search space used: 5237253800
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 62 (29.1 bits)
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