Silkworm BLAST Result against arabidopsis

BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001578-TA|BGIBMGA001578-PA|undefined
         (259 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At3g45040.1 68416.m04852 phosphatidate cytidylyltransferase fami...    85   6e-17
At5g17110.1 68418.m02004 hypothetical protein                          28   5.4  
At1g27940.1 68414.m03423 multidrug resistance P-glycoprotein, pu...    28   5.4  
At3g14810.1 68416.m01871 mechanosensitive ion channel domain-con...    27   9.5  
At1g28010.1 68414.m03430 multidrug resistance P-glycoprotein, pu...    27   9.5  

>At3g45040.1 68416.m04852 phosphatidate cytidylyltransferase family
           protein weak similarity to SP|P20048 Dolichol kinase (EC
           2.7.1.108) {Saccharomyces cerevisiae}; contains Pfam
           profile: PF01148 phosphatidate cytidylyltransferase
          Length = 569

 Score = 84.6 bits (200), Expect = 6e-17
 Identities = 47/193 (24%), Positives = 88/193 (45%), Gaps = 7/193 (3%)

Query: 25  LTLFTIAVIGRTMNILHDPNED-----MNLISSIVL--VQLFAFWLFLLMISVFLVLTRT 77
           L  F +  +   M  +HD N+      +  + S  L  + L  +W+ L+++SV      +
Sbjct: 303 LLFFMVVAVPSWMQFVHDFNQHPFLWVLTFVFSEPLKRLSLCIYWILLIVVSVSRFYNIS 362

Query: 78  KSAVKVNTVTRKSFHLLASLVFMSGIIYDVYLTSXXXXXXXXXXXXVEALRKLNITPISE 137
           +S+     + RK +HL+A L+F+  ++                   +E +R   I P+ E
Sbjct: 363 RSSKVERILLRKYYHLMAVLMFLPALVLQPKFLDLAFGAALAVFVALEIIRIWRIQPLGE 422

Query: 138 ALQSAFDVYSDEKDIGSFAMTPIYLYVGLACPLILVPSYEGTTLELLSGVLATGVGDTAA 197
            L    + ++D +D     ++   L +G A P+ +   +    L   +G+L+ G+GDT A
Sbjct: 423 PLHQFMNAFTDHRDSEHLIVSHFSLLLGCALPIWMSSGFNDRALSPFAGILSLGIGDTMA 482

Query: 198 SWFGSKYGTKKWT 210
           S  G KYG  +W+
Sbjct: 483 SMVGHKYGVLRWS 495



 Score = 31.1 bits (67), Expect = 0.77
 Identities = 13/28 (46%), Positives = 19/28 (67%)

Query: 1   MLRWSLTKFPKTYTIGEAMIVTQGLTLF 28
           ++R  L  FP   +IGEA++VT GL L+
Sbjct: 191 LIRHLLCTFPSCASIGEALLVTSGLVLY 218


>At5g17110.1 68418.m02004 hypothetical protein
          Length = 208

 Score = 28.3 bits (60), Expect = 5.4
 Identities = 22/65 (33%), Positives = 32/65 (49%), Gaps = 3/65 (4%)

Query: 135 ISEALQSAFDVYSDEKDIGSFAMTPIYLYVGLACPLILVPSYEGTTLELLSGVLATGVGD 194
           +S+ L   +DVY DEK  G  +       +   C +      +G TLEL++ V AT  G 
Sbjct: 102 LSDHLNGIYDVYLDEKMDGYNSTRDFMAELANVC-VKKHNETKGNTLELVNVVRATERG- 159

Query: 195 TAASW 199
            AA+W
Sbjct: 160 -AATW 163


>At1g27940.1 68414.m03423 multidrug resistance P-glycoprotein,
           putative similar to mdr-like P-glycoprotein atpgp1
           GI:3849833 from [Arabidopsis thaliana]
          Length = 1245

 Score = 28.3 bits (60), Expect = 5.4
 Identities = 22/76 (28%), Positives = 35/76 (46%), Gaps = 6/76 (7%)

Query: 159 PIYLYVGLACPLILVPSYEGTTLELLSGVLATGVGDTAASWFG-SKYGTKKWTSLLRNNT 217
           PIYL       L+         L L S +L+  +G     WF   +  T   TS+L  + 
Sbjct: 737 PIYLLQHYFYTLMGERLTSRVRLSLFSAILSNEIG-----WFDLDENNTGSLTSILAADA 791

Query: 218 VLIRTMLAASISSVVE 233
            L+R+ LA  +S++V+
Sbjct: 792 TLVRSALADRLSTIVQ 807


>At3g14810.1 68416.m01871 mechanosensitive ion channel
           domain-containing protein / MS ion channel
           domain-containing protein contains Pfam profile PF00924:
           Mechanosensitive ion channel
          Length = 853

 Score = 27.5 bits (58), Expect = 9.5
 Identities = 10/32 (31%), Positives = 22/32 (68%)

Query: 60  AFWLFLLMISVFLVLTRTKSAVKVNTVTRKSF 91
           +FW+FL  IS+ L++T    ++ ++ + RK++
Sbjct: 266 SFWVFLEWISLVLIVTSLVCSLTIHNLQRKTW 297


>At1g28010.1 68414.m03430 multidrug resistance P-glycoprotein,
           putative similar to mdr-like P-glycoprotein GI:3849833
           from [Arabidopsis thaliana]
          Length = 1247

 Score = 27.5 bits (58), Expect = 9.5
 Identities = 23/78 (29%), Positives = 38/78 (48%), Gaps = 14/78 (17%)

Query: 181 LELLSGVLATGVGDTAASWFG-SKYGTKKWTSLLRNNTVLIRTMLAASISSVVEAKTDQV 239
           L L S +L+  +G     WF   +  T   TS+L  +  L+R+ +A  +S++V+      
Sbjct: 761 LSLFSAILSNEIG-----WFDLDENNTGSLTSILAADATLVRSAIADRLSTIVQ------ 809

Query: 240 DNLILPLVTL-LVFQINW 256
            NL L +  L L F  +W
Sbjct: 810 -NLSLTITALALAFFYSW 826


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.324    0.137    0.397 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 4,955,075
Number of Sequences: 28952
Number of extensions: 173892
Number of successful extensions: 574
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 569
Number of HSP's gapped (non-prelim): 10
length of query: 259
length of database: 12,070,560
effective HSP length: 80
effective length of query: 179
effective length of database: 9,754,400
effective search space: 1746037600
effective search space used: 1746037600
T: 11
A: 40
X1: 15 ( 7.0 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 40 (21.6 bits)
S2: 58 (27.5 bits)