Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001558-TA|BGIBMGA001558-PA|IPR002301|Isoleucyl-tRNA
synthetase, class Ia, IPR002300|Aminoacyl-tRNA synthetase, class Ia,
IPR013155|tRNA synthetase, valyl/leucyl, anticodon-binding,
IPR001412|Aminoacyl-tRNA synthetase, class I, IPR009080|Aminoacyl-tRNA
synthetase, class 1a, anticodon-binding
(1212 letters)
Database: arabidopsis
28,952 sequences; 12,070,560 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
At4g10320.1 68417.m01697 isoleucyl-tRNA synthetase, putative / i... 1134 0.0
At5g49030.1 68418.m06067 tRNA synthetase class I (I, L, M and V)... 244 4e-64
At5g16715.1 68418.m01957 tRNA synthetase class I (I, L, M and V)... 123 8e-28
At1g14610.1 68414.m01737 valyl-tRNA synthetase / valine--tRNA li... 93 1e-18
At4g04350.1 68417.m00622 leucyl-tRNA synthetase, putative / leuc... 76 1e-13
At1g09620.1 68414.m01079 tRNA synthetase class I (I, L, M and V)... 43 0.001
At3g55400.1 68416.m06153 methionyl-tRNA synthetase / methionine-... 33 1.2
At4g13780.1 68417.m02137 methionine--tRNA ligase, putative / met... 31 4.7
At4g10930.1 68417.m01778 expressed protein 31 6.2
At1g66210.1 68414.m07515 subtilase family protein contains simil... 31 6.2
>At4g10320.1 68417.m01697 isoleucyl-tRNA synthetase, putative /
isoleucine--tRNA ligase, putative similar to SP|P41252
Isoleucyl-tRNA synthetase, cytoplasmic (EC 6.1.1.5)
(Isoleucine--tRNA ligase) (IleRS) (IRS) {Homo sapiens};
contains Pfam profile PF00133: tRNA synthetases class I
(I, L, M and V)
Length = 1190
Score = 1134 bits (2809), Expect = 0.0
Identities = 564/1080 (52%), Positives = 743/1080 (68%), Gaps = 47/1080 (4%)
Query: 10 ESINFPAEEDKILEFWKKIDAFQTCLKQSKSRPKYSFYDGPPFATGLPHYGHILAGTIKD 69
+ +FP +E+ +L FW +IDAF+T LK++++ P+Y FYDGPPFATGLPHYGHILAGTIKD
Sbjct: 8 KEFSFPRQEEDVLSFWTEIDAFKTQLKRTENLPEYIFYDGPPFATGLPHYGHILAGTIKD 67
Query: 70 VVTRYAHQQGYHVERRFGWDCHGLPVEFEIDKTLGIKGPEDVHKLGIDKYNAECRKIVMK 129
+VTRY G+HV RRFGWDCHGLPVE EID+ L IK ++V K+GIDKYN ECR IV +
Sbjct: 68 IVTRYQTMTGHHVTRRFGWDCHGLPVENEIDRKLNIKRRDEVIKMGIDKYNEECRSIVTR 127
Query: 130 YAADWENIITRMGRWIDFKNDYKTLYPWFMESIWWVFKELYNKGLVYQGVKVMPYSTACS 189
Y A+WE +ITR GRWIDFKNDYKT+ FMES+WWVF +L+ K LVY+G KVMPYST C
Sbjct: 128 YVAEWEKVITRCGRWIDFKNDYKTMDLPFMESVWWVFSQLWEKNLVYRGFKVMPYSTGCK 187
Query: 190 TPLSNFESGQNYKEVVDPAVVVSFPT---DKGYSXXXXXXXXXXXXSNLALCVNPKLIYI 246
TPLSNFE+GQNYKEV DP ++V+FP + SNLALCVN K +Y+
Sbjct: 188 TPLSNFEAGQNYKEVPDPEIMVTFPVIGDQDNAAFVAWTTTPWTLPSNLALCVNAKFVYV 247
Query: 247 KIQENKTGTCYVIQESRFPVIFKNTTDFMILEKFPGE------KLKGIK------YTPVF 294
K++ TG Y++ ESR + + + G+ K KG K V
Sbjct: 248 KVRNKNTGKVYIVAESRLSALPTDKPKAKLSNGPAGDTKKANPKAKGAKPESAADSYEVL 307
Query: 295 DYFVGQCPTA--YQVLTDGY----------VTDD-----SGTGVVHQAPYFGEDDFRVCL 337
+ F G Y+ L D + V DD SGTG+VH AP FGEDD+RVCL
Sbjct: 308 EKFNGASLVGKKYEPLFDYFSDFSSEAFRVVADDYVTDDSGTGIVHCAPAFGEDDYRVCL 367
Query: 338 AAGLITKDQEIICPVDSSGRFTEPVKDFLGQHVKDADKNIIANLKARNRLVQLGQVKHSY 397
+I K + ++ VD G FTE + F G++VKDADK+II +KA+ RLV+ G HSY
Sbjct: 368 LNKIIKKGENLVVAVDDDGLFTERITHFSGRYVKDADKDIIEAVKAKGRLVKTGSFTHSY 427
Query: 398 PFCWRSETPLIYKAVPSWFVRVEQMSLDLLKSSEATNWVPEYVKEKRFGNWLKEARDWAI 457
PFCWRS+TPLIY+AVPSWFVRVEQ+ LLKS+E T WVP YVK+KRF NWL+ ARDWAI
Sbjct: 428 PFCWRSDTPLIYRAVPSWFVRVEQLKEKLLKSNEQTEWVPGYVKDKRFHNWLENARDWAI 487
Query: 458 SRNRYWGTPIPLWISTDKQEVVCIGSIAELQALTGKEITDLHRESIDHLEIPSMRPGQ-P 516
SR+R+WGTP+P+WIS D +EVV + S+ +L+ L+G ++ DLHR IDH+ IPS R +
Sbjct: 488 SRSRFWGTPLPIWISDDGEEVVIMDSVEKLEKLSGVKVFDLHRHHIDHITIPSSRGDEFG 547
Query: 517 PLRRVSEVFDCWFESGSMPYAQCHYPFENKKEFEEIFPANFIAEGIDQTRGWFYTLIVLS 576
LRRV +VFDCWFESGSMPYA HYPFENK+ FE+ FP +F+AEG+DQTRGWFYTL+VLS
Sbjct: 548 VLRRVEDVFDCWFESGSMPYAYIHYPFENKELFEKNFPGDFVAEGLDQTRGWFYTLMVLS 607
Query: 577 TALFKKPPFKNLIANGLVLASDGQKMSKRKKNYPDPLEVVIQYGADALRLYLINSPVVKA 636
TALF+KP FKNLI NGLVLA DG+KM+K+ +NYP PLEV+ +YGADA+RLYLINSPVV+A
Sbjct: 608 TALFEKPAFKNLICNGLVLAEDGKKMAKKLRNYPPPLEVIDEYGADAVRLYLINSPVVRA 667
Query: 637 ENLRFKEEGVRDIVKDVFLPWYNAFRFLMQNVERLVQEDHVDYRFNEKAIRE--NVMDKW 694
E LRFK+EGV +VKDVFLPWYNA+RFL+QN +RL E V + + A + N++D+W
Sbjct: 668 EPLRFKKEGVLGVVKDVFLPWYNAYRFLVQNAKRLETEGGVPFVPTDLATIQSANILDQW 727
Query: 695 ITSFTQSLIQFVKKEMAAYRLYTVVPRLTKFIDHLTNWYVRMNRKRLKGDNGVQDCQVAL 754
I S TQSL++FV++EM AYRLYTVVPRL KF+D+LTN YVR NRKRLKG G DC AL
Sbjct: 728 IHSATQSLVRFVREEMDAYRLYTVVPRLLKFLDNLTNIYVRFNRKRLKGRTGEDDCHTAL 787
Query: 755 DTLFGVLFDMVRVMAPFTPFLTEFMYKTLRKLLPGDSLDSVHFNMIPDPRIELVDTNIER 814
TLF VL +VMAPFTPF TE +Y+ LRK G S +SVH+ IP PR + IE
Sbjct: 788 STLFNVLLTSCKVMAPFTPFFTETLYQNLRKACKG-SEESVHYCSIP-PREGMEGERIEL 845
Query: 815 AVQRMQSVIELGRVLRDRKTIPIKYPLPEMIVIHRDTTYLDDVKS-LEKYVLEEMNVKKL 873
+V RM +I+L R +R+R +P+K PL EMIV+H D +L+D+ L +YVLEE+NV+ L
Sbjct: 846 SVTRMMKIIDLARNIRERNKLPLKTPLKEMIVVHPDADFLNDITGVLREYVLEELNVRSL 905
Query: 874 ELTSDKEKHGITLRAEPDHKILGARLKGDFKAVTQALKSLDNNQCEKIITNGFVELVGQK 933
+D K+ +L+AEPD +LG RL V + +K + G V +
Sbjct: 906 VPCNDTLKYA-SLKAEPDFSVLGKRLGKSMGLVAKEVKEMSQKDILAFEEAGEVTIANHL 964
Query: 934 IDVSEIRL--IFQATG---SDQYEAHSDNDVLILLNVTPDQDMLDEGFSREIINRVQKLR 988
+ ++I++ +F+ ++ ++ D DVL++L++ D +++ GF+REI+NR+QKLR
Sbjct: 965 LKETDIKIVRVFKRPDDLKENEIDSAGDGDVLVILDLRADDSLVEAGFAREIVNRIQKLR 1024
Query: 989 KKAHLVPTDEVDVYF-SVAKTSDILRIINLHRE-FIESTVRAPLIPIEQLSPTKPLIVEE 1046
KK+ L PTD V+VYF S+ + + + + + +E I+ ++ + L+ + L P+ +I+ +
Sbjct: 1025 KKSGLEPTDFVEVYFQSLDEDESVSKQVLVSQEQNIKDSIGSTLL-LSSLMPSHAVIIAD 1083
>At5g49030.1 68418.m06067 tRNA synthetase class I (I, L, M and V)
family protein similar to SP|P41972 Isoleucyl-tRNA
synthetase (EC 6.1.1.5) (Isoleucine--tRNA ligase)
(IleRS) {Staphylococcus aureus}; contains Pfam profile
PF00133: tRNA synthetases class I (I, L, M and V)
Length = 1093
Score = 244 bits (596), Expect = 4e-64
Identities = 215/820 (26%), Positives = 372/820 (45%), Gaps = 79/820 (9%)
Query: 13 NFPAEEDKILEFWKKIDAFQTCLKQSKSRPKYSFYDGPPFATGLPHYGHILAGTIKDVVT 72
N E ++ + W++ F+ + + + + +DGPP+A G H GH L +KD++
Sbjct: 118 NSLTREPELQKLWEENQVFKR-VSDNNNGGSFILHDGPPYANGDLHMGHALNKILKDIIN 176
Query: 73 RYAHQQGYHVERRFGWDCHGLPVEFEIDKTLGIKGPEDVHKLGIDKYNAECRKIVMKYAA 132
RY Q Y V+ GWDCHGLP+E ++ ++L + +++ L K A+ K
Sbjct: 177 RYKLLQNYKVQYVPGWDCHGLPIELKVLQSLDQEVRKELTPL---KLRAKAAKFAKATVK 233
Query: 133 DWENIITRMGRWIDFKNDYKTLYPWFMESIWWVFKELYNKGLVYQGVKVMPYSTACSTPL 192
R G W D+ N Y TL P + + VF ++ KG +Y+G K + +S + T L
Sbjct: 234 TQMESFKRFGVWADWNNPYLTLDPEYEAAQIEVFGQMALKGYIYRGRKPVHWSPSSRTAL 293
Query: 193 SNFESGQNYKE-VVDPAVVVSFPTDKGYSXXXX------------XXXXXXXXSNLALCV 239
+ E+ Y E + ++ F G +N A+ V
Sbjct: 294 A--EAELEYPEGHISKSIYAIFKLVGGAKTSLLDEFIPNIYLAVWTTTPWTMPANAAVAV 351
Query: 240 NPKLIY--IKIQE-NKTGTCYVIQESRFP-VIFKNTTDFMIL---EKFPGEKLK-GIKYT 291
N KL Y +++Q ++ + + + P + KN ++ + P + K G+K +
Sbjct: 352 NAKLQYSVVEVQSFSEDESTVTSNKKKIPGKVLKNQQKLFVIVATDLVPALEAKWGVKLS 411
Query: 292 PVFDYFVG----QCPTAYQV--------LTDGYVTDDSGTGVVHQAPYFGEDDFRVCLAA 339
+ F+G C + + + Y+T +SGTG+VH AP G++D+ L
Sbjct: 412 -ISKTFLGSDLENCRYTHPIDNRDCPVVIGGDYITTESGTGLVHTAPGHGQEDYATGLKY 470
Query: 340 GLITKDQEIICPVDSSGRFTEPVKDFLGQHV-KDADKNIIANLKARNRLVQLGQVKHSYP 398
GL ++ PVD G+FTE F G V + + +++ L LV H YP
Sbjct: 471 GL-----PLVSPVDDEGKFTEEAGQFRGLSVLGEGNTAVVSYLDENMSLVMEESYAHKYP 525
Query: 399 FCWRSETPLIYKAVPSWFVRVEQMSLDLLKSSEATNWVPEYVKEKRFGNWLKEARDWAIS 458
+ WR++ P I++A WF VE + + WVP + R DW IS
Sbjct: 526 YDWRTKKPTIFRATEQWFASVEGFRTATMDAINNVKWVP-HQAVNRISAMTSSRSDWCIS 584
Query: 459 RNRYWGTPIPLWIST-DKQEVVCIGSIAELQALTGKEITDL-HRESIDHLEIPSMRPGQP 516
R R WG PIP + K+ ++ +I ++++ ++ +D S++ L R
Sbjct: 585 RQRTWGVPIPAFYHVKTKEPLMNEETINHVKSIISQKGSDAWWYMSVEDLLPEKYRDKAA 644
Query: 517 PLRRVSEVFDCWFESGSMPYAQCHYPFENKKEFEEIFPANFIAEGIDQTRGWFYTLIVLS 576
+ ++ D WF+SGS +A K+E FPA+ EG DQ RGWF + ++ S
Sbjct: 645 DYEKGTDTMDVWFDSGS-SWA----GVLGKREGLS-FPADVYLEGTDQHRGWFQSSLLTS 698
Query: 577 TALFKKPPFKNLIANGLVLASDGQKMSKRKKNYPDPLEVV---------IQYGADALRLY 627
A K P+ +I +G VL G KMSK N DP V+ YGAD +RL+
Sbjct: 699 IATQGKAPYSAVITHGFVLDEKGMKMSKSLGNVVDPRLVIEGGKNSKDAPAYGADVMRLW 758
Query: 628 LINSPVVKAENLRFKEEGVRDIVKDVFLPWYNAFRFLMQNVERLVQEDHVDYRFNEKAIR 687
+ S V ++ + +R + D++ R+L+ N+ ++ V Y+ + I
Sbjct: 759 V--SSVDYTGDVLIGPQILRQ-MSDIYRKLRGTLRYLLGNLHDWRVDNAVPYQ-DLPIID 814
Query: 688 ENVMDKWITSFTQSLIQFVKKEMAAYRLYTVVPRLTKFIDHLTNWYVRMNRKRL--KGDN 745
++ + + + + ++ IQ + ++++ ++ R T I L+N+Y + + RL G +
Sbjct: 815 QHALFQ-LENVVKN-IQECYENYQFFKIFQIIQRFT--IVDLSNFYFDIAKDRLYTGGTS 870
Query: 746 GV--QDCQVALDTLFGVLFDMVRVMAPFTPFLTEFMYKTL 783
+ CQ L T L ++RV+AP P L E +++ L
Sbjct: 871 SFTRRSCQTVLST---HLLSILRVIAPIVPHLAEDVWQNL 907
>At5g16715.1 68418.m01957 tRNA synthetase class I (I, L, M and V)
family protein similar to SP|P11931 Valyl-tRNA
synthetase (EC 6.1.1.9) (Valine--tRNA ligase) (VALRS)
{Bacillus stearothermophilus}; contains Pfam profile
PF00133: tRNA synthetases class I (I, L, M and V)
Length = 970
Score = 123 bits (296), Expect = 8e-28
Identities = 114/489 (23%), Positives = 206/489 (42%), Gaps = 42/489 (8%)
Query: 307 VLTDGYVTDDSGTGVVHQAPYFGEDDFRVCLAAGLITKDQEIICPVDSSGRFTEPVKDFL 366
++ D YV D GTGV+ +P +D+ + GL P+ + + D
Sbjct: 322 IIADKYVDKDFGTGVLKISPGHDHNDYLLARKLGL---------PILNVMNKDATLNDVA 372
Query: 367 GQHVKDADKNIIANLKARNRLVQLGQVKHSYPFCWRSETPLIYKAVPSWFVRVEQMSLDL 426
G + + + A+L+ V+ P R + WFV ++ ++
Sbjct: 373 GLFWFEVREKLWADLEEIGLAVKKEPHTLRVPRSQRGGEVIEPLVSKQWFVHMDPLAEKA 432
Query: 427 LKSSEATNW--VPEYVKEKRFGNWLKEARDWAISRNRYWGTPIPLW--ISTDKQEVVCIG 482
L + E +PE EK + +WL +DW ISR +WG IP+W + D +E +
Sbjct: 433 LLAVENKELTIIPERF-EKIYNHWLTNIKDWCISRQLWWGHRIPVWYVVGKDCEEDYIVA 491
Query: 483 SIAELQALTGKEITDLHRESIDHLEIPSMRPGQPPLRRVSEVFDCWFESGSMPYAQCHYP 542
AE +AL ++ + + + ++ + P +V D WF S P++ +P
Sbjct: 492 KSAE-EAL--EKALEKYGKDVEIYQDP-------------DVLDTWFSSSLWPFSTLGWP 535
Query: 543 FENKKEFEEIFPANFIAEGIDQTRGWFYTLIVLSTALFKKPPFKNLIANGLVLASDGQKM 602
K+F +P N + G D W ++++ PF ++ +GL+ S G+KM
Sbjct: 536 DVAAKDFNNFYPTNMLETGHDILFFWVARMVMMGIEFTGTVPFSHVYLHGLIRDSQGRKM 595
Query: 603 SKRKKNYPDPLEVVIQYGADALRLYLINSPVVKAENLRFKEEGVRDIVKDVFLPWYNAFR 662
SK N DPL+ + +G DALR + + NL + + +NA +
Sbjct: 596 SKSLGNVIDPLDTIKDFGTDALRFTIALGTAGQDLNLSTERLTANKAFTNKL---WNAGK 652
Query: 663 FLMQNVERL----VQEDHVDYRFN--EKAIRENVMDKWITSFTQSLIQFVKKEMAAYRLY 716
F++ ++ L E+ +D + + E + + + W S LI V +
Sbjct: 653 FVLHSLPSLSDTSAWENLLDLKLDKEETLLSLPLPECWAVSKLHILIDSVTASYEKL-FF 711
Query: 717 TVVPRLTK--FIDHLTNWYVRMNRKRLKGDNGVQDCQVALDTLFGVLFDMVRVMAPFTPF 774
V R T F +WY+ ++ RL G G + L V ++++++ PF PF
Sbjct: 712 GDVGRETYDFFWSDFADWYIEASKSRLYGSGGNSVSLASQAVLLYVFENILKLLHPFMPF 771
Query: 775 LTEFMYKTL 783
+TE +++ L
Sbjct: 772 VTEDLWQAL 780
Score = 74.1 bits (174), Expect = 5e-13
Identities = 50/181 (27%), Positives = 85/181 (46%), Gaps = 3/181 (1%)
Query: 16 AEEDKILEFWKKIDAFQTCLKQSKSRPKYSFYDGPPFATGLPHYGHILAGTIKDVVTRYA 75
+ E+KI ++W+ F+ Q S + PP TG H GH + T++D++ RY
Sbjct: 76 SSEEKIYKWWESQGYFKPNFDQGGS--PFVIPMPPPNVTGSLHMGHAMFVTLEDIMVRYN 133
Query: 76 HQQGYHVERRFGWDCHGLPVEFEIDKTLGIKGPEDVHKLGIDKYNAECRKIVMKYAADWE 135
G G D G+ + ++K L +G + V LG D++ + KY
Sbjct: 134 RMNGRPTLWLPGTDHAGIATQLVVEKMLASEGIKRVD-LGRDEFTKRVWEWKEKYGGTIT 192
Query: 136 NIITRMGRWIDFKNDYKTLYPWFMESIWWVFKELYNKGLVYQGVKVMPYSTACSTPLSNF 195
N I R+G D+ + TL ++ F +L++KGL+YQG ++ +S T +S+
Sbjct: 193 NQIKRLGASCDWSRERFTLDEQLSRAVVEAFVKLHDKGLIYQGSYMVNWSPNLQTAVSDL 252
Query: 196 E 196
E
Sbjct: 253 E 253
>At1g14610.1 68414.m01737 valyl-tRNA synthetase / valine--tRNA
ligase (VALRS) nearly identical to SP|P93736 Valyl-tRNA
synthetase (EC 6.1.1.9) (Valine--tRNA ligase) (ValRS)
{Arabidopsis thaliana}
Length = 1108
Score = 93.1 bits (221), Expect = 1e-18
Identities = 91/380 (23%), Positives = 156/380 (41%), Gaps = 23/380 (6%)
Query: 307 VLTDGYVTDDS-GTGVVHQAPYFGEDDFRVCLAAGLITKDQEIICPVDSSGRFTEPVKDF 365
++ DG + D + GTG V P +D V G K + I D T DF
Sbjct: 406 IICDGILVDPNFGTGCVKITPAHDPNDCEV----GKRHKLEFINIFTDDGKINTNGGSDF 461
Query: 366 LGQHVKDADKNIIANLKARNRLVQLGQVKHSYPFCWRSETPLIYKAVPSWFVRV-----E 420
G A + ++ L+ + + C R+ + P W+V E
Sbjct: 462 AGMPRFAAREAVVEALQKQGLYRGAKNNEMRLGLCSRTNDVIEPMIKPQWYVNCSMIGKE 521
Query: 421 QMSLDLLKSSEATNWVP-EYVKEKRFGNWLKEARDWAISRNRYWGTPIPLWIST-DKQEV 478
+ + + ++ +VP +Y E R WL+ RDW ISR +WG IP W +T ++ ++
Sbjct: 522 ALDVAITDENKKLEFVPKQYTAEWR--RWLENIRDWCISRQLWWGHRIPAWYATLEEDQL 579
Query: 479 VCIGSIAELQALTGKEITDLHRESIDHLEIPSMRPGQPPLRRVSEVFDCWFESGSMPYAQ 538
+G+ ++ + E D E+ L R +V D WF SG P +
Sbjct: 580 KEVGAYSDHWVVARTE-DDAREEAAQKFLGKKFE-----LTRDPDVLDTWFSSGLFPLSV 633
Query: 539 CHYPFENKKEFEEIFPANFIAEGIDQTRGWFYTLIVLSTALFKKPPFKNLIANGLVLASD 598
+P + +F+ +P + + G D W ++++ L + PF + + ++ +
Sbjct: 634 LGWP-DVTDDFKAFYPTSVLETGHDILFFWVARMVMMGMKLGGEVPFSKVYFHPMIRDAH 692
Query: 599 GQKMSKRKKNYPDPLEVVIQYGADALRLYLINSPVVKAENLRFKEEGVRDIVKDVFLPWY 658
G+KMSK N DPLEV+ + L L + E + KE V+D +
Sbjct: 693 GRKMSKSLGNVIDPLEVINGVTLEGLHKRLEEGNLDPKEVIVAKEGQVKDFPNGIPECGT 752
Query: 659 NAFRFLMQNVERLVQEDHVD 678
+A RF + V Q D ++
Sbjct: 753 DALRFAL--VSYTAQSDKIN 770
Score = 71.7 bits (168), Expect = 3e-12
Identities = 43/173 (24%), Positives = 80/173 (46%), Gaps = 2/173 (1%)
Query: 24 FWKKIDAFQTCLKQSKSRPKYSFYDGPPFATGLPHYGHILAGTIKDVVTRYAHQQGYHVE 83
+W+K D F+ K SK P + PP TG H GH L I+D + R+ GY+
Sbjct: 152 WWEKSDLFKADAKSSK--PPFVIVLPPPNVTGALHIGHALTSAIEDTIIRWKRMSGYNAL 209
Query: 84 RRFGWDCHGLPVEFEIDKTLGIKGPEDVHKLGIDKYNAECRKIVMKYAADWENIITRMGR 143
G D G+ + ++K + H +G +++ E K +Y + R+G
Sbjct: 210 WVPGVDHAGIATQVVVEKKIMRDRGMTRHDVGREEFVKEVWKWKNQYGGTILTQLRRLGA 269
Query: 144 WIDFKNDYKTLYPWFMESIWWVFKELYNKGLVYQGVKVMPYSTACSTPLSNFE 196
+D+ + T+ +++ F LY +GL+Y+ ++++ + T +S+ E
Sbjct: 270 SLDWSRECFTMDEQRSKAVTEAFVRLYKEGLIYRDIRLVNWDCILRTAISDVE 322
>At4g04350.1 68417.m00622 leucyl-tRNA synthetase, putative /
leucine--tRNA ligase, putative similar to SP|P36430
Leucyl-tRNA synthetase (EC 6.1.1.4) (Leucine--tRNA
ligase) (LeuRS) {Bacillus subtilis}; contains Pfam
profile PF00133: tRNA synthetases class I (I, L, M and
V)
Length = 973
Score = 76.2 bits (179), Expect = 1e-13
Identities = 55/180 (30%), Positives = 81/180 (45%), Gaps = 18/180 (10%)
Query: 18 EDKILEFWKKIDAFQTCLKQSKSRPKYSFYDGPPFATGLP-HYGHILAGTIKDVVTRYAH 76
E K +W+ F+T S+PK+ D P+ +G H GH L T D++ R
Sbjct: 91 EPKWQRYWEDNRIFRTPDDVDTSKPKFYVLDMFPYPSGAGLHVGHPLGYTATDILARLRR 150
Query: 77 QQGYHVERRFGWDCHGLPVEFEIDKTLGIKGPEDVHKLGIDKYNAECRKIVMKYAADWEN 136
QGY+V GWD GLP E +T G P+ ID++ + + + Y DW+
Sbjct: 151 MQGYNVLHPMGWDAFGLPAEQYAIET-GTH-PKTTTLKNIDRFRLQLKSLGFSY--DWDR 206
Query: 137 IITRMGRWIDFKNDYKTLYPWFMESIWWVFKELYNKGLVYQGVKVMPYSTACSTPLSNFE 196
++ + DY Y W W+F +LY KGL YQ + + A T L+N E
Sbjct: 207 ELSTT------EPDY---YKWTQ----WIFLQLYKKGLAYQAEVPVNWCPALGTVLANEE 253
Score = 44.8 bits (101), Expect = 4e-04
Identities = 31/80 (38%), Positives = 43/80 (53%), Gaps = 8/80 (10%)
Query: 422 MSLDLLKSSEATNWVPEYVKEKRFGNWLKEA----RDWAISRNRYWGTPIPLWISTDKQE 477
+ ++ L S EA V E+ + R GN K+ RDW +R RYWG PIP+ I + E
Sbjct: 479 LDINQLSSKEAALKVIEWAE--RTGNGKKKVNYKLRDWLFARQRYWGEPIPILILDESGE 536
Query: 478 VVCIGSIAELQALTGKEITD 497
+ I S +EL LT E+ D
Sbjct: 537 TIAI-SESEL-PLTLPELND 554
Score = 37.5 bits (83), Expect = 0.054
Identities = 16/27 (59%), Positives = 22/27 (81%)
Query: 601 KMSKRKKNYPDPLEVVIQYGADALRLY 627
KMSK + N +P +VV++YGAD+LRLY
Sbjct: 730 KMSKSRGNVVNPDDVVLEYGADSLRLY 756
>At1g09620.1 68414.m01079 tRNA synthetase class I (I, L, M and V)
family protein similar to cytosolic leucyl-tRNA
synthetase [Candida albicans] GI:9858190; contains Pfam
profile PF00133: tRNA synthetases class I (I, L, M and
V)
Length = 1084
Score = 42.7 bits (96), Expect = 0.001
Identities = 23/86 (26%), Positives = 41/86 (47%), Gaps = 2/86 (2%)
Query: 18 EDKILEFWKKIDAFQTCLKQSKSRPKYSFYDGPPFA--TGLPHYGHILAGTIKDVVTRYA 75
E + ++W+ D F+ ++ +P F+ PF G H GH + + D + Y
Sbjct: 18 EATVRKWWEDEDVFRAESCENLPKPGEKFFSTFPFPYMNGYLHIGHAFSLSKVDFASAYH 77
Query: 76 HQQGYHVERRFGWDCHGLPVEFEIDK 101
+G +V FG+ C G+P++ DK
Sbjct: 78 RLRGANVLLPFGFHCTGMPIKASADK 103
>At3g55400.1 68416.m06153 methionyl-tRNA synthetase /
methionine--tRNA ligase / MetRS (cpMetRS) identical to
methionyl-tRNA synthetase MEtRS [Arabidopsis thaliana]
GI:2266985
Length = 616
Score = 33.1 bits (72), Expect = 1.2
Identities = 14/35 (40%), Positives = 21/35 (60%)
Query: 595 LASDGQKMSKRKKNYPDPLEVVIQYGADALRLYLI 629
L DG KM K N +P E+V ++G DA+R + +
Sbjct: 360 LTKDGMKMGKSLGNTLEPFELVQKFGPDAVRYFFL 394
>At4g13780.1 68417.m02137 methionine--tRNA ligase, putative /
methionyl-tRNA synthetase, putative / MetRS, putative
similar to methionyl-tRNA synthetase [Oryza sativa]
GI:4091008; contains Pfam profiles PF00133: tRNA
synthetases class I (I, L, M and V), PF01588: Putative
tRNA binding domain
Length = 797
Score = 31.1 bits (67), Expect = 4.7
Identities = 15/47 (31%), Positives = 23/47 (48%), Gaps = 1/47 (2%)
Query: 51 PFATGLPHYGHILAGTIK-DVVTRYAHQQGYHVERRFGWDCHGLPVE 96
P+ +PH G+I+ + DV RY +GY+ G D +G E
Sbjct: 26 PYVNNVPHLGNIIGCVLSADVYARYCRLRGYNAIYICGTDEYGTATE 72
>At4g10930.1 68417.m01778 expressed protein
Length = 984
Score = 30.7 bits (66), Expect = 6.2
Identities = 30/138 (21%), Positives = 62/138 (44%), Gaps = 8/138 (5%)
Query: 935 DVSEIRLIFQATGSDQYEAHSDNDVLILLNVTP--DQDMLDEGFSREIINRVQKLRKKAH 992
D S ++++ + ++Q S+ + + +++ D F+ +I NR + +K
Sbjct: 79 DSSHLKILCREENTEQVAVKSELNQSLPHDISSRLSSDSDQPFFAADIENRADPVVEKDC 138
Query: 993 LVPTDEVDVYFSVAKTSDILRIINLHREFIESTVRAPLIPIEQLSPTKPLIVEEAQELKG 1052
L D+ ++ + SD+ +I+L R+ + + + S TKP I E ELK
Sbjct: 139 LSIDDKKNLSTAAISNSDVASVISLKRKHSDCS------GDDGNSETKPEIYESLNELKL 192
Query: 1053 SQLKLVITWRKDVELPSN 1070
+ + + T + PSN
Sbjct: 193 EEEEELTTVHHESRSPSN 210
>At1g66210.1 68414.m07515 subtilase family protein contains
similarity to subtilase; SP1 GI:9957714 from [Oryza
sativa]
Length = 759
Score = 30.7 bits (66), Expect = 6.2
Identities = 22/69 (31%), Positives = 31/69 (44%), Gaps = 5/69 (7%)
Query: 347 EIICPVDSSGRFTEPVKDFLGQHVKDADKNIIANLKARNRLVQLGQVKHSYPFCWRSE-- 404
E++ P+D G T +G V DA+ +A AR + SY CW +E
Sbjct: 219 EVMSPLDKIGHGTHCASTAVGSFVPDANVLSLAQGTARGSAPRARIA--SYKVCWNNEEC 276
Query: 405 -TPLIYKAV 412
TP I KA+
Sbjct: 277 FTPDIVKAI 285
Database: arabidopsis
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 12,070,560
Number of sequences in database: 28,952
Lambda K H
0.320 0.138 0.416
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 29,506,352
Number of Sequences: 28952
Number of extensions: 1319524
Number of successful extensions: 2938
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 3
Number of HSP's that attempted gapping in prelim test: 2898
Number of HSP's gapped (non-prelim): 24
length of query: 1212
length of database: 12,070,560
effective HSP length: 89
effective length of query: 1123
effective length of database: 9,493,832
effective search space: 10661573336
effective search space used: 10661573336
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 65 (30.3 bits)
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