Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001558-TA|BGIBMGA001558-PA|IPR002301|Isoleucyl-tRNA
synthetase, class Ia, IPR002300|Aminoacyl-tRNA synthetase, class Ia,
IPR013155|tRNA synthetase, valyl/leucyl, anticodon-binding,
IPR001412|Aminoacyl-tRNA synthetase, class I, IPR009080|Aminoacyl-tRNA
synthetase, class 1a, anticodon-binding
(1212 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|... 1148 0.0
SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces pombe... 217 8e-57
SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2 |Schizos... 121 5e-28
SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase Vas1|Schizos... 110 1e-24
SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomy... 61 8e-10
SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|c... 44 1e-04
SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein Rad50|Schizos... 37 0.020
SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc... 32 0.56
SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces pomb... 31 0.98
SPBC19F5.04 |||aspartate kinase |Schizosaccharomyces pombe|chr 2... 31 1.3
SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomy... 29 3.9
SPBC1685.15c |klp6|sot2, SPBC649.01c|kinesin-like protein Klp6|S... 29 3.9
SPBC365.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 28 6.9
SPBC1198.11c |reb1|SPBC660.01c|RNA polymerase I transcription te... 28 9.1
SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces... 28 9.1
SPAPB24D3.08c |||NADP-dependent oxidoreductase |Schizosaccharomy... 28 9.1
>SPBC8D2.06 |||isoleucine-tRNA ligase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 1064
Score = 1148 bits (2843), Expect = 0.0
Identities = 550/1060 (51%), Positives = 747/1060 (70%), Gaps = 15/1060 (1%)
Query: 11 SINFPAEEDKILEFWKKIDAFQTCLKQSKSRPKYSFYDGPPFATGLPHYGHILAGTIKDV 70
S N P EE+KI+EFW++IDAF T LK S+ RP Y+F+DGPPFATG PH+GH+LA TIKD
Sbjct: 2 SFNVPKEEEKIVEFWREIDAFHTQLKLSQGRPTYTFFDGPPFATGRPHHGHLLASTIKDS 61
Query: 71 VTRYAHQQGYHVERRFGWDCHGLPVEFEIDKTLGIKGPEDVHKLGIDKYNAECRKIVMKY 130
VTRYA +GYHVERRFGWD HGLPVE EIDK LGI G +DV +GIDKYNAECRKIVM Y
Sbjct: 62 VTRYACLKGYHVERRFGWDTHGLPVEHEIDKKLGITGSDDVMAMGIDKYNAECRKIVMTY 121
Query: 131 AADWENIITRMGRWIDFKNDYKTLYPWFMESIWWVFKELYNKGLVYQGVKVMPYSTACST 190
A++W + R+GRWIDF NDYKTLYP FMES+WWVFKEL+ KG VY+G +VMPYSTAC+T
Sbjct: 122 ASEWRATVERLGRWIDFDNDYKTLYPSFMESVWWVFKELHTKGKVYRGYRVMPYSTACTT 181
Query: 191 PLSNFESGQNYKEVVDPAVVVSFP--TDKGYSXXXXXXXXXXXXSNLALCVNPKLIYIKI 248
PLSNFE+ QNYKEV DPA+VV+F +D S SNLAL V+P L YIKI
Sbjct: 182 PLSNFEAQQNYKEVPDPAIVVAFQSISDPEVSFLAWTTTPWTLPSNLALAVHPDLQYIKI 241
Query: 249 QENKTGTCYVIQESRFPVIFKN--TTDFMILEKFPGEKLKGIKYTPVFDYFVGQC-PTAY 305
+ + Y++ ES +++KN +F ILE+F G+ L G KY P+F YF A+
Sbjct: 242 LDKDSNKKYILMESCLGILYKNPKKANFEILERFQGKALDGQKYEPLFPYFKSTFGERAF 301
Query: 306 QVLTDGYVTDDSGTGVVHQAPYFGEDDFRVCLAAGLITKDQEIICPVDSSGRFTEPVKDF 365
++ + YV + SGTG+VHQAP FGE D+ AAG+I D + CPVD G T + DF
Sbjct: 302 KLYSADYVEEGSGTGIVHQAPAFGEADYDAAWAAGIIDADHQPPCPVDEQGLLTSEITDF 361
Query: 366 LGQHVKDADKNIIANLKARNRLVQLGQVKHSYPFCWRSETPLIYKAVPSWFVRVEQMSLD 425
GQ+VKDADK II +LKA LV+ Q+ HSYPFCWRS+TPLIY+AVPSWFVRV++++ +
Sbjct: 362 AGQYVKDADKEIIRSLKASGHLVKHSQIFHSYPFCWRSDTPLIYRAVPSWFVRVKEITNE 421
Query: 426 LLKSSEATNWVPEYVKEKRFGNWLKEARDWAISRNRYWGTPIPLWISTDKQEVVCIGSIA 485
++++ +T+WVP+ +++KRF NWLK ARDW ISRNRYWGTPIPLW+S D +EVVCIGSI
Sbjct: 422 MVENVMSTHWVPQNIRDKRFANWLKNARDWNISRNRYWGTPIPLWVSDDYEEVVCIGSIK 481
Query: 486 ELQALTG-KEITDLHRESIDHLEIPSMRPGQPPLRRVSEVFDCWFESGSMPYAQCHYPFE 544
EL+ L+G ITD+HR+SIDH+ IPS + G+ L RVSEVFDCWFESGSMPYA HYPFE
Sbjct: 482 ELEELSGVSNITDIHRDSIDHITIPS-KKGKGTLHRVSEVFDCWFESGSMPYASRHYPFE 540
Query: 545 NKKEFEEIFPANFIAEGIDQTRGWFYTLIVLSTALFKKPPFKNLIANGLVLASDGQKMSK 604
+EF+ FPA+FI+EG+DQTRGWFYTL VL T LF K P+KN+I +GLV+A DG+KMSK
Sbjct: 541 RIEEFKHGFPADFISEGVDQTRGWFYTLTVLGTLLFDKAPYKNVIVSGLVMAEDGKKMSK 600
Query: 605 RKKNYPDPLEVVIQYGADALRLYLINSPVVKAENLRFKEEGVRDIVKDVFLPWYNAFRFL 664
R KNYP+P ++ +YG+DALRLYLINSPVV+AE L+FKE+GVR++V V +PW+N+++F
Sbjct: 601 RLKNYPEPNLIIEKYGSDALRLYLINSPVVRAEILKFKEDGVREVVTRVLIPWWNSYKFF 660
Query: 665 MQNVERLVQEDHVDYRFNEKA-IRENVMDKWITSFTQSLIQFVKKEMAAYRLYTVVPRLT 723
+ D+ F++ A + NVMD+WI + QSLI FV +EM YRLYTVVP+L
Sbjct: 661 EAQAALYKKVTGKDFVFDDAATLSSNVMDRWILARCQSLIGFVDEEMKQYRLYTVVPQLL 720
Query: 724 KFIDHLTNWYVRMNRKRLKGDNGVQDCQVALDTLFGVLFDMVRVMAPFTPFLTEFMYKTL 783
I+ +TNWY+R NR+RLKG++G + AL+ LF VLF +VR+M PFTPF+TE +Y+ L
Sbjct: 721 GLIEEMTNWYIRFNRRRLKGEDGEIETINALNVLFEVLFTLVRIMGPFTPFITENIYQHL 780
Query: 784 RKLLPGD----SLDSVHFNMIPDPRIELVDTNIERAVQRMQSVIELGRVLRDRKTIPIKY 839
R +P D SL SVHF P + EL D + R V+RMQ++IEL R +R++ I +K
Sbjct: 781 RNYMPIDKNEISLRSVHFLPFPTYKSELDDETVLRRVKRMQTIIELARYVREQNNISLKT 840
Query: 840 PLPEMIVIHRDTTYLDDVKSLEKYVLEEMNVKKLELTSDKEKHGITLRAEPDHKILGARL 899
PL +IVI + YL+D K LE+Y+ EE+NV+++ TS++EK+G+ + D +LG +L
Sbjct: 841 PLKTLIVILTNEEYLEDAKLLERYIAEELNVREVVFTSNEEKYGVVYSVQADWPVLGKKL 900
Query: 900 KGDFKAVTQALKSLDNNQCEKIITNGFVELVGQKIDVSEIRLIFQATGSDQY-EAHSDND 958
+ D V +AL ++ + + ++ N + L G ++ ++++I +++ ++++D
Sbjct: 901 RKDMARVKKALPNVTSEEVKEFQKNKKMVLDGIELVEGDLQIIRSVEVKNEFLKSNTDGI 960
Query: 959 VLILLNVTPDQDMLDEGFSREIINRVQKLRKKAHLVPTDEVDVYFSVAK-TSDILRIINL 1017
++LL++ D + EG +RE+INRVQ+LRKK++L TD+V + + + T + ++
Sbjct: 961 CIVLLDIEIDAQLQAEGLAREVINRVQRLRKKSNLQVTDDVRMTYKIKNDTIGLESAVDS 1020
Query: 1018 HREFIESTVRAPLIPIEQLSPTKPLIVEEAQELKGSQLKL 1057
+ +R P I E + +I E Q+++G+ L
Sbjct: 1021 NEALFSKVLRRP-IEKETGADESNIIASEEQDVQGATFLL 1059
>SPCC18B5.08c |||isoleucine-tRNA ligase|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 973
Score = 217 bits (530), Expect = 8e-57
Identities = 209/798 (26%), Positives = 344/798 (43%), Gaps = 82/798 (10%)
Query: 23 EFWKKI--DAFQTCLKQSKSRPKYSFYDGPPFATGLPHYGHILAGTIKDVVTRYAHQQGY 80
+++K I D ++ + + DGPPFA G H GH L +KD++ R+ +G
Sbjct: 57 KYFKSITSDLYEWQKENLNKEDSFVLLDGPPFANGRLHIGHALNKILKDIINRWQLLKGR 116
Query: 81 HVERRFGWDCHGLPVEFE-IDKTLGIKGPEDVHKLGIDKYNAECRKIVMKYAADWENIIT 139
V GWDCHGLP+E + I K ++ K+ D N+ ++ +M +
Sbjct: 117 SVHYVPGWDCHGLPIESKAIKANAERKSSLEIRKIAKDFANSAVQEQLMMFQ-------- 168
Query: 140 RMGRWIDFKNDYKTLYPWFMESIWWVFKELYNKGLVYQGVKVMPYSTACSTPLSNFESGQ 199
RM D+ + Y T+ F + VF L K L+++ K + +S++ + L+ E
Sbjct: 169 RMAVMGDWPSRYITMSDKFEIAELKVFLSLLQKDLIFRQNKPVYWSSSSRSALAESEIEY 228
Query: 200 NYKEVVDPAVVVSFPTDK---------GYSXXXXXXXXXXXXSNLALCVNPKLIYIKIQE 250
+ V ++ +FP + SNLAL +P++ Y Q
Sbjct: 229 DDNHV-STSIYFTFPVNSFSIDGCEYNNVKALVWTTTPWTIPSNLALSYHPEINYGLYQH 287
Query: 251 NKTGTCYVIQESRFPVIFKNTTDFMILEKFPGEKLKGIKYTPVFDYFVGQCPT----AYQ 306
N + Y++ ++ P + DFM G K + + F + P ++
Sbjct: 288 NNS--IYLMSDNLVPNL-----DFM-----QGAKRLASCPSDIISSFTYENPLLPKQSFP 335
Query: 307 VLTDGYVTDDSGTGVVHQAPYFGEDDFRVCLAAGLITKDQEIICPVDSSGRFTEPVKD-- 364
L YVT+D GTG+VH AP G +D+ + L L P+D GR+T+ D
Sbjct: 336 FLQSNYVTNDIGTGIVHVAPGHGMEDYLLGLENNL-----RPFSPLDDYGRYTKEALDGS 390
Query: 365 FLGQHVK-DADKNIIANLKARNRLVQLGQVKHSYPFCWRSETPLIYKAVPSWFVRVEQMS 423
G V D K +I+ +K +N +V++ KH YP+ WR+ PLI +A P WF+ +E
Sbjct: 391 LEGLEVLGDGGKKVISIMKNQNMIVKVSPYKHRYPYDWRTHKPLILRATPQWFISLENER 450
Query: 424 LDLLKSSEATNWVPEYVKEKRFGNWLKEARDWAISRNRYWGTPIP-LWISTDKQEVVCIG 482
+K+ ++ +P + + G +L +W ISR R WG PIP L+ K ++ +
Sbjct: 451 KTAIKALDSVKMIPPNSRARLLG-FLNGRPEWCISRQRAWGLPIPVLYEKGTKIPLLTVK 509
Query: 483 SIA---ELQALTGKEITDLHRESIDHLEIPSMRPGQPPLRRVSEVFDCWFESGSMPYAQC 539
S++ E + G + E+ H + R +E D WF+SG+
Sbjct: 510 SVSYIIEKMEVEGVDSWFNDTENNGHAQWVHPDYRNKEYIRGTETLDVWFDSGTSWTTIA 569
Query: 540 HYPFENKKEFEEIFPANFIAEGIDQTRGWFYTLIVLSTALFKKP--PFKNLIANGLVLAS 597
P +NK + EG DQ RGWF +L++ TA KP PF L +G +
Sbjct: 570 --PRKNKPLID------LCLEGSDQHRGWFQSLLLTYTAYQSKPEAPFSTLFTHGFIFDE 621
Query: 598 DGQKMSKRKKNYPDPLEVV---------IQYGADALRLYLINSPVVKAENLRFKEEGVRD 648
GQK SK N DP +V+ + YG D LRL++ + NL +
Sbjct: 622 RGQKQSKSLGNVTDPEDVINGKLLKGKKLPYGVDLLRLWVASCDSTNDTNL---GPNILT 678
Query: 649 IVKDVFLPWYNAFRFLMQNVERLVQEDHVDYRFNEKAIRENVMDKWITSFTQSLIQFVKK 708
V + W RF + N+ VD +R +DK +++
Sbjct: 679 QVGESLKKWRLTSRFCLGNLHDWNTSSSVDV----GELRG--IDKLALVQLDKFQTEIRE 732
Query: 709 EMAAYRLYTVVPRLTKFID-HLTNWYVRMNRKRLKGD--NGVQDCQVALDTLFGVLFDMV 765
+Y + VV L F++ L++ Y + RL D N V V L+ L ++
Sbjct: 733 LYESYSINKVVHHLNYFMNSFLSSTYFDAVKDRLYADLPNSVSRRSVQ-TVLYHSLLTLI 791
Query: 766 RVMAPFTPFLTEFMYKTL 783
++P TP L + ++++L
Sbjct: 792 WAISPITPLLAQEIWQSL 809
>SPBC1709.02c |vas2|SPBC1734.18c|valine-tRNA ligase Vas2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 980
Score = 121 bits (292), Expect = 5e-28
Identities = 133/560 (23%), Positives = 229/560 (40%), Gaps = 57/560 (10%)
Query: 301 CPTAYQVLTDGYVTD-DSGTGVVHQAPYFGEDDFRVCLAAGLITKDQEIICPVDSSGRFT 359
C + ++ D + D + GTG V P +D+ V L E I G
Sbjct: 362 CNRSIPIICDDIIVDMEFGTGAVKITPAHDPNDYEVGKRHNL-----EFINIFTDDGLLN 416
Query: 360 EPVKDFLGQHVKDADKNIIANLKARNRLVQLGQVKHSYPFCWRSETPLIYKAVPSWFVRV 419
E +F G A ++ LK V + P C ++ + P W+V
Sbjct: 417 ENCGEFAGMKRFTARVKVVERLKELGLFVGTKENPMVIPLCGKTSDIIEPVMKPQWWVNQ 476
Query: 420 EQMSL---DLLKSSEATNWVPEYVKEKRFGNWLKEARDWAISRNRYWGTPIPLWISTDKQ 476
++M+ +++KS E + + + F W++ +DW ISR +WG IP +
Sbjct: 477 KEMAAAAAEVVKSGEIE--IAPDMSRREFIRWMENIQDWCISRQLWWGHRIPAYFVNLAD 534
Query: 477 EVVCIGSIAELQALTGKEITDLHRESIDHLEIPSMRPGQP-PLRRVSEVFDCWFESGSMP 535
E S +TG+ + ++ + PG+ L + +V D WF SG P
Sbjct: 535 EPSQDRSEGRYW-VTGRTLEQAEEKA------KAAFPGKSFTLEQDEDVLDTWFSSGLWP 587
Query: 536 YAQCHYPFENKKEFEEIFPANFIAEGIDQTRGWFYTLIVLSTALFKKPPFKNLIANGLVL 595
++ +P ++ ++E +P + G D W +++L L K PFK + + LV
Sbjct: 588 FSTLGWP-KDTSDYENFYPTTLMETGWDILFFWIARMVMLGLKLTGKIPFKRVFCHALVR 646
Query: 596 ASDGQKMSKRKKNYPDPLEVVIQYGADALRLYLI-----NSPVVKAEN------------ 638
+ G+KMSK N DP++V+ AL L+ + V KA+
Sbjct: 647 DAQGRKMSKSLGNVVDPIDVIEGISLQALHDKLLVGNLDSREVEKAKKGQRLSYPKGIPQ 706
Query: 639 -----LRFK----EEGVRDIVKDVF-LPWYNAFRFLMQNVERL----VQEDHVDYRFNEK 684
LRF G RD+ D+ + Y F + N + + + V + +
Sbjct: 707 CGTDALRFTLCSLTTGGRDLNLDILRVEGYRKFCNKLYNATKFALGRLGSNFVPNKTADL 766
Query: 685 AIRENVMDKWITSFTQSLIQFVKKEMAAYRLYTVVPRLTKF-IDHLTNWYVRMNRKRLKG 743
E++++KWI + K M + +F + L + Y+ N K L
Sbjct: 767 TGNESLVEKWIFHRLNIAAAAMNKNMEEMNFLQATSAVHQFWLYELCDVYIE-NSKYLLS 825
Query: 744 DNGVQDCQVALDTLFGVLFDMVRVMAPFTPFLTEFMYKTLRKLLPGDSLDSVHFNMIPDP 803
D + A TL+ VL + +R+M PF P++TE M++ L + PGD ++ P
Sbjct: 826 DGTEVQQESAKQTLYTVLDNALRLMHPFMPYVTEEMWQRLPR-RPGDKTQTIVKAAFP-- 882
Query: 804 RIELVDTNIERAVQRMQSVI 823
+E VD + E A + +S+I
Sbjct: 883 -VERVDYSNEIAAKYYESII 901
Score = 56.8 bits (131), Expect = 2e-08
Identities = 46/190 (24%), Positives = 81/190 (42%), Gaps = 3/190 (1%)
Query: 10 ESINFPAEEDKILEFWKKIDAFQTCLK---QSKSRPKYSFYDGPPFATGLPHYGHILAGT 66
+S N A E ++W K F+ + K + PP TG H GH L
Sbjct: 95 KSYNPKAVESAWYDWWVKSGFFEPEFGPDGKPKKEGVFVITSPPPNVTGALHIGHALTIA 154
Query: 67 IKDVVTRYAHQQGYHVERRFGWDCHGLPVEFEIDKTLGIKGPEDVHKLGIDKYNAECRKI 126
I+D + R+ G V G+D GL + ++K L + H DK+ +
Sbjct: 155 IQDSLARWNRMLGKTVLFLGGFDHAGLSTQSVVEKKLWYTQKKTRHDYPRDKFVDIVWEW 214
Query: 127 VMKYAADWENIITRMGRWIDFKNDYKTLYPWFMESIWWVFKELYNKGLVYQGVKVMPYST 186
+Y +N ++R+G D+ + T+ ++ F L+ + ++Y+ +++ + T
Sbjct: 215 KEEYHNRIKNQMSRLGGSFDWTREAFTMDENLSRAVVETFVRLHEENIIYRANRLVNWCT 274
Query: 187 ACSTPLSNFE 196
A T LSN E
Sbjct: 275 ALQTTLSNLE 284
>SPAC4A8.08c |vas1||mitochondrial valine-tRNA ligase
Vas1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 950
Score = 110 bits (264), Expect = 1e-24
Identities = 86/313 (27%), Positives = 140/313 (44%), Gaps = 39/313 (12%)
Query: 307 VLTDGYVTDDSGTGVVHQAPYFGEDDFRVCLAAGLITKDQEIICPVDSSGRFTEPVKDFL 366
V+ D V GTGV+ P D+ + + + + +D SG K+
Sbjct: 295 VICDNAVDRHFGTGVLKITPMHSIVDYEIAKRHNI-----DCVSIMDKSGNLINCSKEVN 349
Query: 367 GQHVKDADKNIIANLKARNRLVQLGQVKHSY--PFCWRSETPLIYKAVPSWFVRVEQMSL 424
G + A I+ L+ RNRLV+ QV HS C R+ + VP W++ V+ +
Sbjct: 350 GMNRLKARSKIVRLLQQRNRLVE--QVPHSLILSVCSRTGDVIEPVMVPQWYLSVDSLKK 407
Query: 425 DLLKSSEATNWVPEYVKEKRFGNWLKEARDWAISRNRYWGTPIPLWISTDKQEVVCIGSI 484
++LKSS VP + K + +W K+ DW ISR +WG IP+W ++ + + +
Sbjct: 408 EVLKSSNKLKLVPSLAR-KEWDSWFKKMGDWCISRQIWWGHQIPVWKILEEDKWIAAPNY 466
Query: 485 AE-LQALTGKEITDLHRESIDHLEIPSMRPGQPPLRRVSEVFDCWFESGSMPYAQCHYPF 543
+ LQ GK ++ + S+V D WF S +P + +P
Sbjct: 467 EKALQLSVGKSVS-----------------------QDSDVLDTWFSSALLPLSAFGWP- 502
Query: 544 ENKKEFEEIFPANFIAEGIDQTRGWFYTLIVLSTALFKKPPFKNLIANGLVLASDGQKMS 603
+ ++I P FI G D W + +L + PFK +I + LV S+G+KMS
Sbjct: 503 ----KSKDIQPLPFIESGQDILFFWIARMALLCKYFSNELPFKEIILHPLVRDSEGRKMS 558
Query: 604 KRKKNYPDPLEVV 616
K N DP++++
Sbjct: 559 KSLGNVIDPMDII 571
Score = 58.8 bits (136), Expect = 4e-09
Identities = 42/160 (26%), Positives = 71/160 (44%), Gaps = 1/160 (0%)
Query: 37 QSKSRPKYSFYDGPPFATGLPHYGHILAGTIKDVVTRYAHQQGYHVERRFGWDCHGLPVE 96
+S P + PP TG H GH L TI+D + R+ GY V R G D G+ +
Sbjct: 53 KSIEAPMFPILLPPPNITGKLHIGHALTITIQDALARFYAMHGYKVSFRPGTDHAGIATQ 112
Query: 97 FEIDKTLGIKGPEDVHKLGIDKYNAECRKIVMKYAADWENIITRMGRWIDFKNDYKTLYP 156
++K L KG ++L D+ + +KY N + D+ N + T+
Sbjct: 113 SVVEKYLQKKGVYR-NQLSKDELLSSIHSWQVKYQKSIINQLKSFEAIFDWDNIFYTMDQ 171
Query: 157 WFMESIWWVFKELYNKGLVYQGVKVMPYSTACSTPLSNFE 196
E++ F L+N GL+Y+ + + + + +S+ E
Sbjct: 172 NRSEAVNEAFISLFNAGLIYRANRFVNWCPKLESAVSDIE 211
>SPAC4G8.09 |||mitochondrial leucine-tRNA ligase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 874
Score = 61.3 bits (142), Expect = 8e-10
Identities = 50/175 (28%), Positives = 75/175 (42%), Gaps = 19/175 (10%)
Query: 21 ILEFWKKI-DAFQTCLKQSKSRPKYSFYDGPPFATGLPHYGHILAGTIKDVVTRYAHQQG 79
I E WK + +K K + KY P+ +GL H GH+ TI D+++RY +G
Sbjct: 37 IAENWKSYWKSHYPFVKNDKGKKKY-ILSMFPYPSGLLHIGHVRVYTISDILSRYYRMKG 95
Query: 80 YHVERRFGWDCHGLPVEFEIDKTLGIKGPEDVHKLGIDKYNAECRKIVMKYAADWENIIT 139
Y V GWD GLP E + GI ++ I K + M DW+ I+
Sbjct: 96 YKVIHPMGWDAFGLPAENAAIEN-GISASSWTYE-NIKKMKEQTYH--MNIYFDWDREIS 151
Query: 140 RMGRWIDFKNDYKTLYPWFMESIWWVFKELYNKGLVYQGVKVMPYSTACSTPLSN 194
DY Y W ++F ++++KGL YQ + + T L+N
Sbjct: 152 TC------NPDY---YKWSQ----YLFLQMFHKGLAYQAEATVNWDPVDCTVLAN 193
Score = 41.9 bits (94), Expect = 5e-04
Identities = 17/36 (47%), Positives = 24/36 (66%), Gaps = 2/36 (5%)
Query: 436 VPEYVKEKRFGNWLKEAR--DWAISRNRYWGTPIPL 469
+ Y++EK+ +K R DW ISR R+WGTPIP+
Sbjct: 409 IVSYLEEKKLAKRVKNYRLKDWLISRQRFWGTPIPM 444
Score = 38.3 bits (85), Expect = 0.006
Identities = 17/36 (47%), Positives = 24/36 (66%)
Query: 600 QKMSKRKKNYPDPLEVVIQYGADALRLYLINSPVVK 635
QKMSK K N DP+ + +YG+D +R Y+I S V+
Sbjct: 627 QKMSKSKHNGVDPIRTIQKYGSDIIRAYIIFSAPVE 662
Score = 29.5 bits (63), Expect = 3.0
Identities = 18/58 (31%), Positives = 26/58 (44%), Gaps = 1/58 (1%)
Query: 401 WRSETPLIYKAVPSWFVRVEQMSLDLLKSSEATNWVPEYVKEKRFGNWLKEARDWAIS 458
WRS + K + WF+++ S LL E P+ VK K NW+ + IS
Sbjct: 203 WRSGAIVEKKNLRQWFLKISDYSDQLLDDLETLPKWPDKVK-KMQRNWIGRTTGFEIS 259
>SPAC26F1.13c |||leucine-tRNA ligase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1111
Score = 44.4 bits (100), Expect = 1e-04
Identities = 41/152 (26%), Positives = 71/152 (46%), Gaps = 8/152 (5%)
Query: 547 KEFEEIFPANFIAEGIDQTRGWFYTLIVLSTALFKKPPF-KNLIANGLVLASDGQKMSKR 605
+EF+ +P + A G D + TA+F + + K + ANG +L + G+KMSK
Sbjct: 683 REFQYFYPFDIRASGKDLVPNHLTFCLYTHTAIFDEKYWPKGIRANGHLLMN-GEKMSKS 741
Query: 606 KKNYPDPLEVVIQYGADALRLYLINSPVVKAENLRFKEEGVRDIVKDVFLPWYNAFRFLM 665
N+ E ++GADA RL L ++ ++ F+E + L Y +
Sbjct: 742 TGNFMTLHEATKKFGADATRLALADAGDT-VDDANFEEA----LANSAILRLYTQEAWCK 796
Query: 666 QNVERLVQEDHVDYRFNEKAIRENVMDKWITS 697
+ +E L Y F++K + EN +++ I S
Sbjct: 797 EMMENLDNLRTGPYNFHDK-VFENEINQLIES 827
Score = 29.9 bits (64), Expect = 2.3
Identities = 18/64 (28%), Positives = 28/64 (43%), Gaps = 2/64 (3%)
Query: 39 KSRPKYSFYDGP-PFATGLPHYGHILAGTIKDVVTRYAHQQGYHVERRFGWDCHGLPVEF 97
K PK+ F + P P+ G H GH + + T + G V G+ C G+P+
Sbjct: 62 KKYPKF-FGNMPYPYMNGALHLGHAFTLSKVEFTTAFERLNGKRVLFPMGFHCTGMPICA 120
Query: 98 EIDK 101
D+
Sbjct: 121 SADR 124
>SPAC1556.01c |rad50|SPAP4C9.01c|DNA repair protein
Rad50|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1290
Score = 36.7 bits (81), Expect = 0.020
Identities = 30/111 (27%), Positives = 53/111 (47%), Gaps = 5/111 (4%)
Query: 811 NIERAVQRMQSVIELGRVLR--DRKTIPIKYPLPEMIVIHR-DTTYLDDVKSLEK--YVL 865
N+E+ ++ + L +R DR+T ++ LP I H D Y + K LEK Y+
Sbjct: 775 NLEKLQLLVKDISNLEEEIRTIDRETEVLRIELPSSIAHHNLDEIYAEREKLLEKRGYLR 834
Query: 866 EEMNVKKLELTSDKEKHGITLRAEPDHKILGARLKGDFKAVTQALKSLDNN 916
+++ KLE TS K+K + A + K+ +L + Q K ++ +
Sbjct: 835 KQIERTKLEETSFKKKIDDAVLANNEQKLKLTKLNFQVNELEQLEKDINKS 885
>SPAC27E2.06c |||methionine-tRNA ligase,
mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
Length = 539
Score = 31.9 bits (69), Expect = 0.56
Identities = 26/90 (28%), Positives = 39/90 (43%), Gaps = 3/90 (3%)
Query: 541 YPFENKKE-FEEIFPANFIAEGIDQTRGWFYTLIVLSTALFKKPPFKNLIANGLVLASDG 599
YP+ N+K +PAN G D R F+ + + + P I +
Sbjct: 267 YPWLNEKSSLSAGWPANMHVIGKDIIR--FHCIYWPAFLMAAGLPLPEKILVHSHWTMNK 324
Query: 600 QKMSKRKKNYPDPLEVVIQYGADALRLYLI 629
KMSK N DP ++ +YG D +R YL+
Sbjct: 325 VKMSKSLGNVVDPFWLIEKYGVDTIRYYLL 354
Score = 29.9 bits (64), Expect = 2.3
Identities = 21/70 (30%), Positives = 33/70 (47%), Gaps = 3/70 (4%)
Query: 38 SKSRPKYSFYDGPPF-ATGLPHYGHILAGTIKDVVTRYAH-QQGYHVERRFGWDCHGLPV 95
S+S K F P F PH GH+ + + D + R+ + + V G D HGL V
Sbjct: 14 SESSKKPYFLTTPIFYVNAAPHLGHLYSLVLTDAIARFQNLKPDVSVISSTGTDEHGLKV 73
Query: 96 EFEIDKTLGI 105
+ + +T G+
Sbjct: 74 Q-TVAQTEGV 82
>SPBC17A3.04c |||methionine-tRNA ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 782
Score = 31.1 bits (67), Expect = 0.98
Identities = 20/57 (35%), Positives = 30/57 (52%), Gaps = 10/57 (17%)
Query: 422 MSLDLLKSSEATNWVPEYVKEKRFGN--------WLKEA-RDWAISRNRYWGTPIPL 469
+SLD L+ + +W + E ++ N WLKE R I+R+ WGTP+PL
Sbjct: 410 LSLDKLQPA-VESWAMQSAVEGKWSNNGRSITESWLKEGLRPRCITRDLKWGTPVPL 465
Score = 29.5 bits (63), Expect = 3.0
Identities = 21/87 (24%), Positives = 44/87 (50%), Gaps = 7/87 (8%)
Query: 51 PFATGLPHYGHILAGTIK-DVVTRYAHQQGYHVERRFGWDCHGLPVEFEIDKTLGIKGPE 109
P+ +PH G+I+ T+ DV RY + ++ G D +G E + + G+ P+
Sbjct: 231 PYVNNVPHLGNIVGSTLSADVFARYHRARNHNTLYICGTDEYGTATETKALEE-GV-SPK 288
Query: 110 DVHKLGIDKYNAECRKIVMKYAADWEN 136
++ DKY+A +++ + D+++
Sbjct: 289 EL----CDKYHALHKEVYDWFEIDFDH 311
>SPBC19F5.04 |||aspartate kinase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 519
Score = 30.7 bits (66), Expect = 1.3
Identities = 17/66 (25%), Positives = 33/66 (50%), Gaps = 3/66 (4%)
Query: 958 DVLILLNVTPDQDMLDEGFSREIINRVQKLRKKAHLVPTDEVDVYFSVAKTSDILRIINL 1017
D ++++N+ ++ + GF I + K + L+ T EV V ++ + SD N+
Sbjct: 356 DTIMVINIQSNRKISAHGFLASIFAILDKYKLAVDLITTSEVHVSMALYEESD---DGNM 412
Query: 1018 HREFIE 1023
H F+E
Sbjct: 413 HEAFVE 418
>SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 754
Score = 29.1 bits (62), Expect = 3.9
Identities = 31/115 (26%), Positives = 51/115 (44%), Gaps = 7/115 (6%)
Query: 576 STALFKKPPFKNLIANGLVLASDGQKMSKRKKNYPDPLEVVIQYGADALRL-YLINSPVV 634
S A F P + N + L +GQKMSK KN+ E++ ++ LRL +L+
Sbjct: 383 SEAYFDCPQWVNYFFHAGHLHIEGQKMSKSLKNFITIKEILKKFTPRQLRLAFLLQQ--- 439
Query: 635 KAENLRFKEEGVRDI--VKDVFLPWYNAFRFLMQNVERL-VQEDHVDYRFNEKAI 686
L FKE + + ++ ++ R LM E + HV +F++ I
Sbjct: 440 WNTQLDFKETLLAYVLNIEQALENFFRTVRALMNETEGVSANGGHVPEKFDKLEI 494
>SPBC1685.15c |klp6|sot2, SPBC649.01c|kinesin-like protein
Klp6|Schizosaccharomyces pombe|chr 2|||Manual
Length = 784
Score = 29.1 bits (62), Expect = 3.9
Identities = 25/82 (30%), Positives = 40/82 (48%), Gaps = 6/82 (7%)
Query: 936 VSEIRLIFQATGSDQYEAHSDNDVLILLNVTPDQDMLD-----EGFSREIINRVQKLRKK 990
+S I F+ G+D Y +DV +L ++ +Q +LD E FS +Q L
Sbjct: 538 ISHIVSSFKGEGADMYADMLQDDVDLLKSIIENQ-ILDAKHESETFSSTSRKLIQNLFLL 596
Query: 991 AHLVPTDEVDVYFSVAKTSDIL 1012
L+P + +DV S+A+ D L
Sbjct: 597 FPLLPGNAIDVNESLARAFDQL 618
>SPBC365.04c |||RNA-binding protein|Schizosaccharomyces pombe|chr
2|||Manual
Length = 233
Score = 28.3 bits (60), Expect = 6.9
Identities = 16/45 (35%), Positives = 26/45 (57%), Gaps = 4/45 (8%)
Query: 858 KSLEKYVLEEMNVKKLELTSDKEKHGITLRAEPDHKIL-GARLKG 901
K+ +K +E+ K+L ++DK+KH + + P HK L R KG
Sbjct: 44 KAADKISRDELPEKQLAQSNDKDKHSV---SNPPHKTLKSKRQKG 85
>SPBC1198.11c |reb1|SPBC660.01c|RNA polymerase I transcription
termination factor Reb1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 504
Score = 27.9 bits (59), Expect = 9.1
Identities = 20/98 (20%), Positives = 45/98 (45%), Gaps = 4/98 (4%)
Query: 668 VERLVQEDHVDYRFNEKAIRENVMDKWITSFTQSLIQFVKKEMAAYRLYTVVPRLTKFID 727
+ER +Q Y + + +++ +K + SL++ + +EM ++ T++ L +
Sbjct: 164 LERRMQNFCQTYSLDHTQVADSLHEKRLHGPLSSLVKLLVQEMPSFTRRTILRHLRALYN 223
Query: 728 HLTNWYVRMNRKRL--KGDNGVQDCQVALDTLFGVLFD 763
Y + +RK +GD GVQ+ + + + D
Sbjct: 224 --IPGYEKYSRKNSSGRGDFGVQETAIISQEVHNFIMD 259
>SPBC23G7.06c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 745
Score = 27.9 bits (59), Expect = 9.1
Identities = 12/29 (41%), Positives = 17/29 (58%)
Query: 1003 FSVAKTSDILRIINLHREFIESTVRAPLI 1031
F KTS++L +INLH + V+ LI
Sbjct: 128 FDPVKTSELLHVINLHEILYQKLVKQGLI 156
>SPAPB24D3.08c |||NADP-dependent oxidoreductase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 349
Score = 27.9 bits (59), Expect = 9.1
Identities = 16/55 (29%), Positives = 25/55 (45%), Gaps = 3/55 (5%)
Query: 35 LKQSKSRPKYSFYDGPPFATGLPHYGHILAGTIKDVVTRYAHQQGYHVERRFGWD 89
L+ PK++ Y PP G P Y +A +K + +Y + G V GW+
Sbjct: 59 LRMRMQSPKHASYI-PPLELGKPFYNSTVAKVVKSTLDQY--KPGMDVVFVSGWE 110
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.320 0.138 0.416
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 5,822,035
Number of Sequences: 5004
Number of extensions: 264074
Number of successful extensions: 696
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 10
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 642
Number of HSP's gapped (non-prelim): 39
length of query: 1212
length of database: 2,362,478
effective HSP length: 81
effective length of query: 1131
effective length of database: 1,957,154
effective search space: 2213541174
effective search space used: 2213541174
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 59 (27.9 bits)
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