Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001558-TA|BGIBMGA001558-PA|IPR002301|Isoleucyl-tRNA
synthetase, class Ia, IPR002300|Aminoacyl-tRNA synthetase, class Ia,
IPR013155|tRNA synthetase, valyl/leucyl, anticodon-binding,
IPR001412|Aminoacyl-tRNA synthetase, class I, IPR009080|Aminoacyl-tRNA
synthetase, class 1a, anticodon-binding
(1212 letters)
Database: nematostella
59,808 sequences; 16,821,457 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SB_19663| Best HMM Match : No HMM Matches (HMM E-Value=.) 445 e-125
SB_14622| Best HMM Match : DUF1441 (HMM E-Value=1.6) 339 7e-93
SB_9076| Best HMM Match : Gas_vesicle_C (HMM E-Value=0.9) 62 4e-09
SB_18770| Best HMM Match : No HMM Matches (HMM E-Value=.) 55 3e-07
SB_609| Best HMM Match : tRNA-synt_1 (HMM E-Value=2.2e-09) 52 4e-06
SB_57601| Best HMM Match : No HMM Matches (HMM E-Value=.) 39 0.021
SB_25659| Best HMM Match : No HMM Matches (HMM E-Value=.) 36 0.26
SB_50408| Best HMM Match : No HMM Matches (HMM E-Value=.) 35 0.35
SB_48268| Best HMM Match : No HMM Matches (HMM E-Value=.) 35 0.46
SB_22570| Best HMM Match : Filament (HMM E-Value=0.1) 34 0.80
SB_55369| Best HMM Match : EMP70 (HMM E-Value=0.026) 30 9.9
>SB_19663| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 579
Score = 445 bits (1097), Expect = e-125
Identities = 212/407 (52%), Positives = 274/407 (67%), Gaps = 36/407 (8%)
Query: 105 IKGPEDVHKLGIDKYNAECRKIVMKYAADWENIITRMGRWIDFKNDYKTLYPWFMESIWW 164
IKGP+DV K+GI KYN ECRKIVM+Y+ DWE I++R+GRWIDF+NDYKTLYPWFME++WW
Sbjct: 64 IKGPDDVAKMGIAKYNDECRKIVMRYSTDWERIVSRLGRWIDFENDYKTLYPWFMETVWW 123
Query: 165 VFKELYNKGLVYQGVKVMPYSTACSTPLSNFESGQNYKEVVDPAVVVSFPTDKG--YSXX 222
VFK+L+ K +VY+G KVMPYST C+TPLSNFES QNYKEV DPAV VSFP D+ S
Sbjct: 124 VFKQLHEKNMVYRGFKVMPYSTVCNTPLSNFESNQNYKEVQDPAVFVSFPLDESPDVSMI 183
Query: 223 XXXXXXXXXXSNLALCVNPKLIYIKIQENKTGTCYVIQESRFPVIFKNTTDFMILEKFPG 282
SNLAL VN + Y+K+++N T Y++ +R +FK ++ +LE+F G
Sbjct: 184 AWTTTPWTLPSNLALVVNKDMEYVKVKDNSTEKVYIMMAARLDALFKTPEEYTVLERFKG 243
Query: 283 EKLKGIKYTPVFDYFVG-QCPTAYQVLTDGYVTDDSGTGVVHQAPYFGEDDFRVCLAAGL 341
L+G Y P+F YF + A+++LTD YVT++SGTGVVH AP FGEDD+RV +A G+
Sbjct: 244 ASLEGKTYKPMFPYFEQLKSSGAFRILTDSYVTEESGTGVVHSAPAFGEDDYRVSMANGV 303
Query: 342 ITKDQEIICPVDSSGRFTEPVKDFLGQHVKDADKNIIANLKARNRLVQLGQVKHSYPFCW 401
I K + CPVD++G+FT V DF GQ+VKDADK+II LK RLV +KH+YPFCW
Sbjct: 304 IHKGGSLPCPVDANGKFTAEVTDFAGQYVKDADKHIIKWLKQHRRLVHQSTIKHNYPFCW 363
Query: 402 RSETPLIYKAVPSWFVRVEQMSLDLLKSSEATNWVPEYVKEKRFGNWLKEARDWAISRNR 461
R VP + +K NW L+ A DW +SRNR
Sbjct: 364 R---------VP-----------EFVKEKRFHNW-------------LQNAHDWNVSRNR 390
Query: 462 YWGTPIPLWISTDKQEVVCIGSIAELQALTGKEITDLHRESIDHLEI 508
YWGTPIPLW+S D QEVVC+GS+ EL L+G +TDLHRE++ + ++
Sbjct: 391 YWGTPIPLWVSDDYQEVVCVGSVDELAELSGVRLTDLHRENMPYAQV 437
Score = 169 bits (411), Expect = 1e-41
Identities = 73/94 (77%), Positives = 87/94 (92%)
Query: 533 SMPYAQCHYPFENKKEFEEIFPANFIAEGIDQTRGWFYTLIVLSTALFKKPPFKNLIANG 592
+MPYAQ HYPFE K++FE+ FPA+FIAEGIDQTRGWFYTL+V+STALF KPPFKNLI NG
Sbjct: 431 NMPYAQVHYPFERKEDFEKTFPADFIAEGIDQTRGWFYTLLVISTALFDKPPFKNLIVNG 490
Query: 593 LVLASDGQKMSKRKKNYPDPLEVVIQYGADALRL 626
LVLA+DG KMSKRKKNYPDP+E++ ++G+DALRL
Sbjct: 491 LVLAADGAKMSKRKKNYPDPMEIINKHGSDALRL 524
Score = 109 bits (263), Expect = 1e-23
Identities = 46/61 (75%), Positives = 54/61 (88%)
Query: 8 VPESINFPAEEDKILEFWKKIDAFQTCLKQSKSRPKYSFYDGPPFATGLPHYGHILAGTI 67
V E NFP EE+KILE WKK+DAF++ L+QS+ RP+Y+FYDGPPFATGLPHYGHILAGTI
Sbjct: 5 VSEQHNFPKEEEKILELWKKLDAFKSSLRQSEGRPRYTFYDGPPFATGLPHYGHILAGTI 64
Query: 68 K 68
K
Sbjct: 65 K 65
>SB_14622| Best HMM Match : DUF1441 (HMM E-Value=1.6)
Length = 426
Score = 339 bits (834), Expect = 7e-93
Identities = 198/434 (45%), Positives = 270/434 (62%), Gaps = 57/434 (13%)
Query: 626 LYLINSPVVKAENLRFKEEGVRDIVKDVFLPWYNAFRFLMQNVERLVQEDHVDYRFNEKA 685
LYLINSPVV+AE L+F+E+GV+D+VKDVFLPWYNA+RFL Q++++ + +V++ ++E
Sbjct: 2 LYLINSPVVRAETLKFQEKGVKDVVKDVFLPWYNAYRFLSQSIDQFQRVHNVNFAYDENN 61
Query: 686 IRE--NVMDKWITSFTQSLIQFVKKEMAAYRLYTVVPRLTKFIDHLTNWYVRMNRKRLKG 743
E N+MDKWI SFTQSL+QFV +EM AYRLYTVVPRL KFI++LTNWYVR NRKRLKG
Sbjct: 62 TMELHNIMDKWILSFTQSLVQFVHQEMDAYRLYTVVPRLVKFIEYLTNWYVRFNRKRLKG 121
Query: 744 DNGVQDCQVALDTLFGVLFDMVRVMAPFTPFLTEFMYKTLRKLLPGDSL----DSVHFNM 799
D G +D A+ TLFGV+ + ++MAPFTPFLTE +Y+ ++ + D DS+H+ M
Sbjct: 122 DQGQEDAMQAIQTLFGVVLTITKLMAPFTPFLTEHIYQNMKPFIVFDESEKENDSIHYLM 181
Query: 800 IPDPRIELVDTNIERAVQRMQSVIELGRVLRDRKTIPIKYPLPEMIVIHRDTTYLDDVKS 859
+P R L+ T IERAV RMQ+ IELGRV+RDR TIP K E+ V R T D
Sbjct: 182 VPKTRENLIHTEIERAVSRMQATIELGRVVRDRMTIPTK----ELNV--RKVTVSSD--- 232
Query: 860 LEKYVLEEMNVKKLELTSDKEKHGITLRAEPDHKILGARLKGDFKA----------VTQA 909
DK G+ LRAEPD + LG RLKG FK + Q
Sbjct: 233 -----------------KDK--FGLRLRAEPDSQTLGRRLKGAFKQAPFTPFLTEHIYQN 273
Query: 910 LKS---LDNNQCEK------IITNGFVELVGQKID--VSEIRLIFQATGSDQYEAHSDND 958
+K D ++ E ++ L+ +I+ VS ++ + +
Sbjct: 274 MKPFIVFDESEKENDSIHYLMVPKTRENLIHTEIERAVSRMQATIELGRVVRDRMTIPTK 333
Query: 959 VLILLNVTPDQDMLDEGFSREIINRVQKLRKKAHLVPTDEVDVYFSVAKTSD--ILRIIN 1016
+LILL+VTPDQ MLDEG +RE +NR+QKLRKKA L P D+V + + V + + IIN
Sbjct: 334 ILILLDVTPDQKMLDEGLAREFVNRIQKLRKKAGLQPVDDVSIVYEVEPSGSQRLGEIIN 393
Query: 1017 LHREFIESTVRAPL 1030
H+E+I+++V+ P+
Sbjct: 394 AHKEYIQTSVKQPV 407
>SB_9076| Best HMM Match : Gas_vesicle_C (HMM E-Value=0.9)
Length = 305
Score = 61.7 bits (143), Expect = 4e-09
Identities = 66/318 (20%), Positives = 137/318 (43%), Gaps = 24/318 (7%)
Query: 584 PFKNLIANGLVLASDGQKMSKRKKNYPDPLEVVIQYGADALRL-YLINSPVVKAENLRFK 642
PF + G+V G+KMSK+ N PD L ++ ++GADA+R +I+SP A N
Sbjct: 11 PFDKVYFTGIVRDKQGRKMSKQLGNSPDLLALIEEHGADAVRFSVMISSP---AGNDLLY 67
Query: 643 EEGVRDIVKDVFLPWYNAFRFLMQNVERLVQEDHVDYRFNEKAIRENVMDKWITSFTQSL 702
+E + ++ +NA + + R + D E+ + M++ + +Q +
Sbjct: 68 DESSLEQGRNFSNKMWNALKLIKSWEGRTTK----DVAIGEQQFAVDWMEQRVAKASQQI 123
Query: 703 IQFVKKEMAAYRLYTVVPRLTKFIDHLTNWYVRMNRKRLKGDNGVQDCQVALDTLFGVLF 762
+ +K + L T+ + D +WY+ +K Q +
Sbjct: 124 EELMKDFRLSEGLKTIYSLIW---DDFCSWYLEW----VKPAQEASMSQELYERTVATFE 176
Query: 763 DMVRVMAPFTPFLTEFMYKTLRKLLPGDSLDSVHFNMIPDPRIELVDTNIERAVQRMQSV 822
+++++ P+ PF+TE MY + + GD L P V+ N+ + +Q +
Sbjct: 177 QLLQLLHPYMPFVTEEMYHLMNERKDGDDLILKQL-----PSFSTVNENLLKEGALLQQL 231
Query: 823 IELGRVLRDRKTIPIKYPLPEMIVIHRDTTYLDDVKSLEKYVLEEMNVKKLELTSDKEKH 882
I R R++ + K + I + DTT ++ + ++N +++ T++ ++
Sbjct: 232 ITSVRDARNKNQLKPK----DTIKLWVDTTNEQFYTLVKPMLSRQINAEEIAFTTEAKQG 287
Query: 883 GITLRAEPDHKILGARLK 900
I++ D + A ++
Sbjct: 288 TISVVVGTDKLYIEANVE 305
>SB_18770| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 154
Score = 55.2 bits (127), Expect = 3e-07
Identities = 27/94 (28%), Positives = 47/94 (50%), Gaps = 1/94 (1%)
Query: 523 EVFDCWFESGSMPYAQCHYPFENKKEFEEIFPANFIAEGIDQTRGWFYTLIVLSTALFKK 582
+V D WF SG P++ +P E + + +P + G D W ++ + L K
Sbjct: 27 DVLDTWFSSGLFPFSIFGWP-EKTDDLQVFYPGTLLETGHDILFFWVARMVKMGLKLTGK 85
Query: 583 PPFKNLIANGLVLASDGQKMSKRKKNYPDPLEVV 616
PF+ + + +V + G+KMSK N DP++V+
Sbjct: 86 LPFREVYLHAMVRDAHGRKMSKSLGNVIDPVDVI 119
>SB_609| Best HMM Match : tRNA-synt_1 (HMM E-Value=2.2e-09)
Length = 250
Score = 51.6 bits (118), Expect = 4e-06
Identities = 59/231 (25%), Positives = 96/231 (41%), Gaps = 22/231 (9%)
Query: 115 GIDKYNAECRKIVMKYAADWENI--------ITRMGRWIDFKNDYKTLYPWFMESIWWVF 166
GIDK NA R+ ++YA +W+ + ++G +D+ + T+ + ++ VF
Sbjct: 31 GIDK-NALSREEFLEYAWEWKEKYGGIILQQLKKLGCALDWNRTHFTMDDEYYAAVIRVF 89
Query: 167 KELYNKGLVYQGVKVMPYSTACSTPLSNFESGQNYKEVVDPAVVVSFPTDKGYSXXXXXX 226
+LYNKG +Y+GVK++ + T LS+ E V DK
Sbjct: 90 VDLYNKGQIYRGVKMINWDPKAKTALSDEEVIHKQTNSKLYYVRYKLDGDKEEYITIATV 149
Query: 227 XXXXXXSNLALCVNPK-LIYIKIQENKTGTCYV-IQESRFPVIFKNTTDFMILEKFPGEK 284
+ A+CV+P Y ++ K C+V + P+IF + D +F
Sbjct: 150 RPETILGDTAICVHPNDERYAHLKGRK---CFVPLVNREIPIIFDDYIDI----EFGTGA 202
Query: 285 LKGIKYTPVFDYFVGQCPTAYQVLTDGYVTDDSGTGVVHQAPYFGEDDFRV 335
LK + DY +GQ + T +D GT Y G+D F V
Sbjct: 203 LKVTPAHDINDYNLGQKHNLEVIDT----LNDDGTLSEAAGMYVGQDRFVV 249
>SB_57601| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 161
Score = 39.1 bits (87), Expect = 0.021
Identities = 38/151 (25%), Positives = 70/151 (46%), Gaps = 19/151 (12%)
Query: 600 QKMSKRKK-NYPDPLEVVIQYGADALRLYLINSPVVKAENLRF---KEEGVRDIVKDVFL 655
+K++K+ K +P E + YG DALR + + +++F + EG R+ +
Sbjct: 7 EKIAKQTKAEFP---EGIAAYGTDALRFTNL-ALASTGRDIKFDMGRVEGYRNFCNKI-- 60
Query: 656 PWYNAFRFLMQNVERLVQEDHVDYRFNEKAIRENVMDKWITSFTQSLIQFVKKEMAAYRL 715
+NA F+++N D D N + + + +D+WI S Q V +++ A+R
Sbjct: 61 --WNAANFVIENT------DGQDTGINGEEVELSSVDRWIISALQRTEAEVTRQLDAFRF 112
Query: 716 YTVVPRLTKFI-DHLTNWYVRMNRKRLKGDN 745
L +FI D WY+ + + L +N
Sbjct: 113 DLAAHALYEFIWDEYCAWYLELVKPVLWDEN 143
>SB_25659| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 314
Score = 35.5 bits (78), Expect = 0.26
Identities = 30/85 (35%), Positives = 42/85 (49%), Gaps = 16/85 (18%)
Query: 1120 GLKYTLWGDGSEVKDTNN--LNTKTI---IASAAAPTPHDSKTVPA--CKFVNV------ 1166
G +T++ S V DTN + T+ + + AP P D TVP C+FVNV
Sbjct: 179 GKPFTVFSAQSMVSDTNTRPYSAPTVNGHVTQSKAPIPADKPTVPTPVCRFVNVQLVGVS 238
Query: 1167 -ANGTKS--FTLFLENPLGKETLDR 1188
A G++ T+ LENP G+ L R
Sbjct: 239 PACGSRGQCATVLLENPRGEFILSR 263
>SB_50408| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 550
Score = 35.1 bits (77), Expect = 0.35
Identities = 19/49 (38%), Positives = 28/49 (57%), Gaps = 3/49 (6%)
Query: 428 KSSEATNWVPEYVKEKRFGNWLKEA-RDWAISRNRYWGTPIPLWISTDK 475
KS+ NW P + R +W++E + I+R+ WGTP+PL TDK
Sbjct: 130 KSTNEGNWSPNARQITR--SWIQEGLKPRCITRDLKWGTPVPLEGYTDK 176
>SB_48268| Best HMM Match : No HMM Matches (HMM E-Value=.)
Length = 4527
Score = 34.7 bits (76), Expect = 0.46
Identities = 16/44 (36%), Positives = 24/44 (54%)
Query: 277 LEKFPGEKLKGIKYTPVFDYFVGQCPTAYQVLTDGYVTDDSGTG 320
+EKF ++ TPVF++ G+ P QV TD Y +G+G
Sbjct: 8 VEKFDEKRYFLKNITPVFNFAAGESPLMSQVFTDAYSATTTGSG 51
>SB_22570| Best HMM Match : Filament (HMM E-Value=0.1)
Length = 601
Score = 33.9 bits (74), Expect = 0.80
Identities = 20/85 (23%), Positives = 48/85 (56%), Gaps = 9/85 (10%)
Query: 804 RIELVDTNIERAVQRMQSVIELGRVLRDRKTIPIKYPLP------EMIVIHRDTTYLDDV 857
+++ +D E+ +++M++ + + LR+R + I+Y E++ ++ YLD
Sbjct: 29 KLQDIDDEYEQRIEKMKA--DHAKALRERSQV-IEYQTDSTRKATEILEKSKELEYLDKE 85
Query: 858 KSLEKYVLEEMNVKKLELTSDKEKH 882
+ +KYVL+E+ + +LT ++E +
Sbjct: 86 YTKQKYVLDELQERLNDLTQERENY 110
>SB_55369| Best HMM Match : EMP70 (HMM E-Value=0.026)
Length = 370
Score = 30.3 bits (65), Expect = 9.9
Identities = 24/99 (24%), Positives = 46/99 (46%), Gaps = 6/99 (6%)
Query: 353 DSSGRFTEPVKD---FLGQHVKDADKNIIANLKAR---NRLVQLGQVKHSYPFCWRSETP 406
DS+G FT+ +++ + ++ K +++ + + + ++ HS P C S TP
Sbjct: 230 DSAGSFTKRIRETHFLIDMNLSSKVKKCLSSQDEKQPSSGTQGVAKIGHSLPVCPISATP 289
Query: 407 LIYKAVPSWFVRVEQMSLDLLKSSEATNWVPEYVKEKRF 445
+ + VRV Q S DLL + + Y++ RF
Sbjct: 290 WVPEDAVQREVRVIQKSRDLLAHVLYSTPISSYMQGPRF 328
Database: nematostella
Posted date: Oct 22, 2007 1:22 PM
Number of letters in database: 16,821,457
Number of sequences in database: 59,808
Lambda K H
0.320 0.138 0.416
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 41,090,067
Number of Sequences: 59808
Number of extensions: 1786160
Number of successful extensions: 3389
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 6
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 3368
Number of HSP's gapped (non-prelim): 17
length of query: 1212
length of database: 16,821,457
effective HSP length: 90
effective length of query: 1122
effective length of database: 11,438,737
effective search space: 12834262914
effective search space used: 12834262914
T: 11
A: 40
X1: 16 ( 7.4 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.8 bits)
S2: 65 (30.3 bits)
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