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Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= BGIBMGA001554-TA|BGIBMGA001554-PA|IPR007174|Las1-like
         (144 letters)

Database: arabidopsis 
           28,952 sequences; 12,070,560 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

At5g12220.1 68418.m01434 las1-like family protein similar to Las...    90   5e-19
At3g43550.1 68416.m04626 GDSL-motif lipase, putative similar to ...    30   0.70 
At5g66460.1 68418.m08381 (1-4)-beta-mannan endohydrolase, putati...    29   1.6  
At3g43570.1 68416.m04631 GDSL-motif lipase, putative similar to ...    28   2.1  
At1g62660.1 68414.m07071 beta-fructosidase (BFRUCT3) / beta-fruc...    27   4.9  
At2g36010.3 68415.m04422 E2F transcription factor-3 (E2F3) ident...    27   6.5  
At2g36010.1 68415.m04420 E2F transcription factor-3 (E2F3) ident...    27   6.5  
At2g23260.1 68415.m02778 UDP-glucoronosyl/UDP-glucosyl transfera...    27   6.5  
At1g11950.1 68414.m01381 transcription factor jumonji (jmjC) dom...    27   6.5  
At1g59406.1 68414.m06678 GDSL-motif lipase, putative similar to ...    26   8.6  
At1g59030.1 68414.m06668 GDSL-motif lipase, putative similar to ...    26   8.6  

>At5g12220.1 68418.m01434 las1-like family protein similar to Las1p
           [Saccharomyces cerevisiae] GI:495504; contains Pfam
           profile PF04031: Las1-like
          Length = 611

 Score = 90.2 bits (214), Expect = 5e-19
 Identities = 48/149 (32%), Positives = 91/149 (61%), Gaps = 10/149 (6%)

Query: 4   CVVVPWVNTEEWLKVMNLIYSENSDE-NEALKWLLLWKARCPSLPSGIESTLILLQVHIQ 62
           C  VPW++ EEW  V   ++S + D    AL+ +  W++R  SLP+ ++ T  L+++ ++
Sbjct: 28  CKQVPWLSWEEWDSVRESLFSSSPDRIASALERVATWRSR-GSLPAPVDVTCSLIEIQLK 86

Query: 63  D------LNSPDDNGNDHVLRLAYSSAIMRFINHMLDVSITKEN-TLSKAAKNVGIPDWI 115
           D        S D   ++H+L++ Y   I+R +N +++ +  +E+ +++ AA+ +GIP  +
Sbjct: 87  DGFIPREKQSADALYSEHLLQMLYCMGILRLVNCVIEKTRRREDVSIADAARAIGIPRKL 146

Query: 116 VELRHDTAHNNNLPSLSLLRDATQFGLDW 144
           ++LRH+ +H   LP+LS+LRDA    L+W
Sbjct: 147 IDLRHEGSH-RELPALSVLRDAADEALEW 174


>At3g43550.1 68416.m04626 GDSL-motif lipase, putative similar to
           family II lipase EXL3 (GI:15054386), EXL1 (GI:15054382),
           EXL2 (GI:15054384) [Arabidopsis thaliana]; contains Pfam
           profile PF00657: Lipase/Acylhydrolase with GDSL-like
           motif
          Length = 349

 Score = 29.9 bits (64), Expect = 0.70
 Identities = 19/40 (47%), Positives = 22/40 (55%), Gaps = 2/40 (5%)

Query: 94  VSITKENTLSKAAKNVGIPDWIV--ELRHDTAHNNNLPSL 131
           V I  E    K  KNV IP  IV  +   DT +NNNLP+L
Sbjct: 11  VLIAVETYAVKQGKNVTIPALIVFGDSIMDTGNNNNLPTL 50


>At5g66460.1 68418.m08381 (1-4)-beta-mannan endohydrolase, putative
           similar to (1-4)-beta-mannan endohydrolase [Coffea
           arabica] GI:10178872; contains Pfam profile PF00150:
           Cellulase (glycosyl hydrolase family 5)
          Length = 431

 Score = 28.7 bits (61), Expect = 1.6
 Identities = 13/40 (32%), Positives = 24/40 (60%), Gaps = 2/40 (5%)

Query: 28  DENEALKWLLLWKARCPSLPSG--IESTLILLQVHIQDLN 65
           D+   + W L+ + RCPS PSG  I++ +  +  H++ L+
Sbjct: 191 DDPTIMAWELMNEPRCPSDPSGRAIQAWITEMAAHVKSLD 230


>At3g43570.1 68416.m04631 GDSL-motif lipase, putative similar to
           family II lipases EXL3 GI:15054386, EXL1 GI:15054382,
           EXL2 GI:15054384 from [Arabidopsis thaliana]; contains
           Pfam profile PF00657: GDSL-like Lipase/Acylhydrolase
          Length = 320

 Score = 28.3 bits (60), Expect = 2.1
 Identities = 18/40 (45%), Positives = 21/40 (52%), Gaps = 2/40 (5%)

Query: 94  VSITKENTLSKAAKNVGIPDWIV--ELRHDTAHNNNLPSL 131
           V I  E    K  KN  IP  IV  +   DT +NNNLP+L
Sbjct: 11  VLIVVEANAVKQGKNATIPALIVFGDSIMDTGNNNNLPTL 50


>At1g62660.1 68414.m07071 beta-fructosidase (BFRUCT3) /
           beta-fructofuranosidase / invertase, vacuolar identical
           to beta-fructosidase GB:CAA67560 GI:1429209 [Arabidopsis
           thaliana]; supported by full-length cDNA GI:14517549;
           identical to cDNA  Beta-fructosidase GI:3115854
          Length = 648

 Score = 27.1 bits (57), Expect = 4.9
 Identities = 10/26 (38%), Positives = 18/26 (69%)

Query: 11  NTEEWLKVMNLIYSENSDENEALKWL 36
           +T+E+++V NL Y E+  +   LKW+
Sbjct: 198 STDEFVQVQNLAYPEDPSDPLLLKWV 223


>At2g36010.3 68415.m04422 E2F transcription factor-3 (E2F3)
           identical to E2F transcription factor-3 E2F3
           [Arabidopsis thaliana] gi|10443853|gb|AAG17610
          Length = 485

 Score = 26.6 bits (56), Expect = 6.5
 Identities = 24/75 (32%), Positives = 33/75 (44%), Gaps = 4/75 (5%)

Query: 27  SDENEALKWLLLWKARCPSLPSGIESTLILLQV-HIQDLNSPD-DNGNDHVLRLAYSSAI 84
           S+  +  KWL + +    SLP     TLI ++  H   L  PD D   DH  R  Y   I
Sbjct: 278 SENEKNQKWLFVTEEDIKSLPGFQNQTLIAVKAPHGTTLEVPDPDEAADHPQR-RY-RII 335

Query: 85  MRFINHMLDVSITKE 99
           +R     +DV +  E
Sbjct: 336 LRSTMGPIDVYLVSE 350


>At2g36010.1 68415.m04420 E2F transcription factor-3 (E2F3)
           identical to E2F transcription factor-3 E2F3
           [Arabidopsis thaliana] gi|10443853|gb|AAG17610
          Length = 483

 Score = 26.6 bits (56), Expect = 6.5
 Identities = 24/75 (32%), Positives = 33/75 (44%), Gaps = 4/75 (5%)

Query: 27  SDENEALKWLLLWKARCPSLPSGIESTLILLQV-HIQDLNSPD-DNGNDHVLRLAYSSAI 84
           S+  +  KWL + +    SLP     TLI ++  H   L  PD D   DH  R  Y   I
Sbjct: 276 SENEKNQKWLFVTEEDIKSLPGFQNQTLIAVKAPHGTTLEVPDPDEAADHPQR-RY-RII 333

Query: 85  MRFINHMLDVSITKE 99
           +R     +DV +  E
Sbjct: 334 LRSTMGPIDVYLVSE 348


>At2g23260.1 68415.m02778 UDP-glucoronosyl/UDP-glucosyl transferase
           family protein contains Pfam profile: PF00201
           UDP-glucoronosyl and UDP-glucosyl transferase
          Length = 456

 Score = 26.6 bits (56), Expect = 6.5
 Identities = 16/55 (29%), Positives = 27/55 (49%), Gaps = 1/55 (1%)

Query: 63  DLNSPDDNGNDHVLRLAYSSAIMRFINHMLDVSITKENTLSKAAKNVGIPD-WIV 116
           D    DD   + + + A SS +      ML+    +  T++KA KN G+P  W++
Sbjct: 251 DFCKSDDCCMEWLDKQARSSVVYISFGSMLETLENQVETIAKALKNRGLPFLWVI 305


>At1g11950.1 68414.m01381 transcription factor jumonji (jmjC)
           domain-containing protein contains Pfam domain, PF02373:
           jmjC domain; non-consensus TG acceptor splice site at
           exon boundary 79262
          Length = 880

 Score = 26.6 bits (56), Expect = 6.5
 Identities = 12/44 (27%), Positives = 21/44 (47%), Gaps = 2/44 (4%)

Query: 13  EEWLKVMNLIYSENSDENEALKW--LLLWKARCPSLPSGIESTL 54
           E W K   +I     +    L W  +++W+A C ++ S I S +
Sbjct: 498 EHWSKGEPVIVRNALNNTAGLSWEPMVMWRALCENVDSAISSNM 541


>At1g59406.1 68414.m06678 GDSL-motif lipase, putative similar to
           family II lipase EXL3 GI:15054386 from [Arabidopsis
           thaliana]; contains Pfam profile PF00657: GDSL-like
           Lipase/Acylhydrolase
          Length = 349

 Score = 26.2 bits (55), Expect = 8.6
 Identities = 17/40 (42%), Positives = 21/40 (52%), Gaps = 2/40 (5%)

Query: 94  VSITKENTLSKAAKNVGIPDWIV--ELRHDTAHNNNLPSL 131
           V I  E   +   KN  IP  IV  +   DT +NNNLP+L
Sbjct: 11  VLIFVEANAATQGKNTTIPALIVFGDSIMDTGNNNNLPTL 50


>At1g59030.1 68414.m06668 GDSL-motif lipase, putative similar to
           family II lipase EXL3 GI:15054386 from [Arabidopsis
           thaliana]; contains Pfam profile PF00657: GDSL-like
           Lipase/Acylhydrolase
          Length = 349

 Score = 26.2 bits (55), Expect = 8.6
 Identities = 17/40 (42%), Positives = 21/40 (52%), Gaps = 2/40 (5%)

Query: 94  VSITKENTLSKAAKNVGIPDWIV--ELRHDTAHNNNLPSL 131
           V I  E   +   KN  IP  IV  +   DT +NNNLP+L
Sbjct: 11  VLIFVEANAATQGKNTTIPALIVFGDSIMDTGNNNNLPTL 50


  Database: arabidopsis
    Posted date:  Oct 3, 2007  3:31 PM
  Number of letters in database: 12,070,560
  Number of sequences in database:  28,952
  
Lambda     K      H
   0.317    0.132    0.412 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,671,872
Number of Sequences: 28952
Number of extensions: 140519
Number of successful extensions: 356
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 9
Number of HSP's that attempted gapping in prelim test: 351
Number of HSP's gapped (non-prelim): 11
length of query: 144
length of database: 12,070,560
effective HSP length: 75
effective length of query: 69
effective length of database: 9,899,160
effective search space: 683042040
effective search space used: 683042040
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
S2: 55 (26.2 bits)

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