Last updated: 2022/11/18
BLASTP 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= BGIBMGA001525-TA|BGIBMGA001525-PA|IPR008994|Nucleic
acid-binding, OB-fold, IPR002307|Tyrosyl-tRNA synthetase, class Ib,
IPR002547|t-RNA-binding region, IPR002305|Aminoacyl-tRNA synthetase,
class Ib
(365 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1672.05c |||tyrosine-tRNA ligase |Schizosaccharomyces pombe|... 231 1e-61
SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA syntheta... 143 4e-35
SPBC16G5.08 |trp4||phosphoribosylanthranilate transferase Trp4|S... 30 0.45
SPAC23A1.04c |mnl1||alpha mannosidase-like protein|Schizosacchar... 30 0.59
SPAC17H9.07 |||signal recognition particle subunit Srp21 |Schizo... 28 1.8
SPBC428.01c |nup107|SPBC582.11c|nucleoporin Nup107|Schizosacchar... 27 4.2
SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6 |Schizosaccharom... 27 4.2
SPCC663.03 |pmd1||leptomycin efflux transporter Pmd1|Schizosacch... 26 7.3
SPAC16C9.06c |upf1||ATP-dependent RNA helicase Upf1|Schizosaccha... 26 9.6
SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain |Schizosaccha... 26 9.6
>SPCC1672.05c |||tyrosine-tRNA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 401
Score = 231 bits (565), Expect = 1e-61
Identities = 113/200 (56%), Positives = 145/200 (72%), Gaps = 15/200 (7%)
Query: 3 TWQEKKHLITRNLQEVLGDDKLTEILKQRDLKIYWGTATTGRPHVAYFVPMLKIADFLKA 62
T EK LITRNLQEVLG + EIL +RD+ +YWG+A TGRPH YFVPM+K+ADFLKA
Sbjct: 4 TPDEKYELITRNLQEVLGAKMIREILNERDISLYWGSAPTGRPHCGYFVPMMKLADFLKA 63
Query: 63 GCEVTILFADLHAYLDNMKAPWELLALRTQYYEAAIKAMLTSIGVPLEKLKFVRGTEYQL 122
G VT+L AD+HA+LDNMKAP EL+ R +YYE +K++L S+ +P+EKL+FV G+ YQL
Sbjct: 64 GVHVTVLLADVHAFLDNMKAPMELVQHRVRYYEIIVKSILKSLNIPIEKLRFVIGSSYQL 123
Query: 123 SKEYTLDVYRLSSVVTEHDAKKAGAEVVK---------------PTLDEEYLKVDAQFGG 167
++YTLD +RL++ TEH A++AGAEVVK LDE+YL VD QFGG
Sbjct: 124 KEDYTLDNFRLAASTTEHTARRAGAEVVKQVASPLLSSLIYPGMQALDEQYLGVDVQFGG 183
Query: 168 VDQRKIFAMSEKFLRLIRVK 187
VDQRKIF M+E+ L ++ K
Sbjct: 184 VDQRKIFVMAEEVLPVLGYK 203
>SPAC30C2.04 |||cofactor for methionyl-and glutamyl-tRNA synthetases
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 450
Score = 143 bits (346), Expect = 4e-35
Identities = 64/137 (46%), Positives = 93/137 (67%), Gaps = 1/137 (0%)
Query: 199 PSKLDIRVGHIVEVSRHPDADALYVEKIDVGEDE-PRTIVSGLVNFVPIEEMQDRDVVVL 257
PS +D R+G I + +HP+AD+LYV I G+ E PRT+ SGLV ++P+E+MQ R V+V+
Sbjct: 279 PSMIDFRIGFIEKAVKHPNADSLYVSTIHCGDAEGPRTVCSGLVKYIPLEQMQQRKVIVV 338
Query: 258 CNLKAAKMRGVESKGMVLCASVDEPKQVEPLLPPKGTKAGDRIVVEEYDTGEPDEVLNPK 317
NLK MR V+S+ MV CAS + VE +LPP+ + GDR+ E +DT EP+ LNPK
Sbjct: 339 ANLKPVNMRSVKSQAMVFCASSPDKSVVEFVLPPENAEIGDRLTFEGFDTEEPEAQLNPK 398
Query: 318 KKVWEKLQADLKTNDNL 334
+K+WE +Q + ++L
Sbjct: 399 RKIWEAIQPGFTSGEDL 415
>SPBC16G5.08 |trp4||phosphoribosylanthranilate transferase
Trp4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 354
Score = 30.3 bits (65), Expect = 0.45
Identities = 16/65 (24%), Positives = 31/65 (47%)
Query: 88 ALRTQYYEAAIKAMLTSIGVPLEKLKFVRGTEYQLSKEYTLDVYRLSSVVTEHDAKKAGA 147
A+ + YEAA++A+LT P+E F+ ++E + + ++ + A
Sbjct: 22 AIPLENYEAALRAILTGKASPVETASFLASLHLTKAEEVPDILMQTVQILKSYSTPIANI 81
Query: 148 EVVKP 152
E+V P
Sbjct: 82 EMVSP 86
>SPAC23A1.04c |mnl1||alpha mannosidase-like
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 787
Score = 29.9 bits (64), Expect = 0.59
Identities = 12/37 (32%), Positives = 20/37 (54%)
Query: 85 ELLALRTQYYEAAIKAMLTSIGVPLEKLKFVRGTEYQ 121
ELL L T+ E + A T G+P ++ ++G Y+
Sbjct: 161 ELLTLATELAERLLPAFRTPTGIPFARINLMKGVAYR 197
>SPAC17H9.07 |||signal recognition particle subunit Srp21
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 120
Score = 28.3 bits (60), Expect = 1.8
Identities = 13/31 (41%), Positives = 18/31 (58%)
Query: 269 ESKGMVLCASVDEPKQVEPLLPPKGTKAGDR 299
E + V+ + V E K+ EP PPKG+K R
Sbjct: 86 EPEQEVVASPVTEQKKAEPSAPPKGSKKKKR 116
>SPBC428.01c |nup107|SPBC582.11c|nucleoporin
Nup107|Schizosaccharomyces pombe|chr 2|||Manual
Length = 794
Score = 27.1 bits (57), Expect = 4.2
Identities = 36/147 (24%), Positives = 69/147 (46%), Gaps = 13/147 (8%)
Query: 5 QEKKHLITRNLQEVLGDDKLTEILKQRDLKIYWGTATTGRPHVAYFV--PMLKIAD---- 58
+E H + + LQ + +K++EIL +++ T P V P+L+I
Sbjct: 389 EEASHPLIK-LQTHIICNKISEILSSAHIQLE-AIRTGNAPESGDLVTPPLLRILTHIIL 446
Query: 59 FLK-AGCEVTILFAD--LHAYLDNMKAPWE--LLALRTQYYEAAIKAMLTSIGVPLEKLK 113
FLK +G V +D + AY++ + + + L+ L QY ++ S + L +
Sbjct: 447 FLKISGLAVDEYTSDSIIQAYIELLASAKKVNLVPLYIQYLSNQVQYEAYSRFLILVDEE 506
Query: 114 FVRGTEYQLSKEYTLDVYRLSSVVTEH 140
R + QL+K+Y+LD+ + + E+
Sbjct: 507 SARSEQLQLAKKYSLDINHAALLAVEY 533
>SPBC211.04c |mcm6|mis5|MCM complex subunit Mcm6
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 892
Score = 27.1 bits (57), Expect = 4.2
Identities = 31/127 (24%), Positives = 54/127 (42%), Gaps = 8/127 (6%)
Query: 147 AEVVKPTLDEEYLKVDAQFGGVDQRKIFAMS-EKFLRLIRVKGNINQAVDDVTPSKLDIR 205
AE+VK ++ DAQ G + +I E +RL N VDD+TP+ ++
Sbjct: 672 AEIVKKY--KQLRMDDAQGAGKNSYRITVRQLESMIRLSEAIARAN-CVDDITPAFVNEA 728
Query: 206 VGHIVEVSRHPDADALYVEKIDVG----EDEPRTIVSGLVNFVPIEEMQDRDVVVLCNLK 261
+ + H + D + VE+ D E++ +G N E +Q V + +
Sbjct: 729 YSLLRQSIIHVERDDIEVEEDDAEAQELENDNTNTTNGNDNVSSEEALQKPKVKITYDKY 788
Query: 262 AAKMRGV 268
+ M G+
Sbjct: 789 VSIMNGI 795
>SPCC663.03 |pmd1||leptomycin efflux transporter
Pmd1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1362
Score = 26.2 bits (55), Expect = 7.3
Identities = 15/42 (35%), Positives = 25/42 (59%), Gaps = 4/42 (9%)
Query: 306 DTGEPDEVLNPKKKVWEKLQADLKTNDNLTAVWQVHDAIRTV 347
D GE +E PKK +K + + ++LTA+W +H +RT+
Sbjct: 752 DVGELNE--EPKKS--KKSKKNNHEINSLTALWFIHSFVRTM 789
>SPAC16C9.06c |upf1||ATP-dependent RNA helicase
Upf1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 925
Score = 25.8 bits (54), Expect = 9.6
Identities = 17/65 (26%), Positives = 29/65 (44%), Gaps = 7/65 (10%)
Query: 21 DDKLTEILKQRDLKIYWGTATTGRPHVAYFVPMLKIADF-LKAGCEVTILFADLHAYLDN 79
D +L E Q+D+ + W A R + +P L+ + L G E+ + Y
Sbjct: 238 DKRLKESQTQKDVVVRWDQAINKRYTAWFLLPKLESGEIRLAIGDEMKL------TYEGE 291
Query: 80 MKAPW 84
++APW
Sbjct: 292 LRAPW 296
>SPAC1093.06c |dhc1|SPAC30C2.01c|dynein heavy chain
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 4196
Score = 25.8 bits (54), Expect = 9.6
Identities = 17/60 (28%), Positives = 31/60 (51%), Gaps = 3/60 (5%)
Query: 170 QRKIFAMSEKFLRLIRVKGNINQAVDDVTPSKLDIRVGHIVE---VSRHPDADALYVEKI 226
Q+K F ++EK +++ AV + S DI+ H++E +SR P A + +E +
Sbjct: 3179 QKKSFLLNEKKNSVMKEVSYAKPAVIEARKSVSDIKKAHLIELRSLSRPPMAIRITMEVV 3238
Database: spombe
Posted date: Oct 3, 2007 3:31 PM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.317 0.135 0.389
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,655,283
Number of Sequences: 5004
Number of extensions: 68846
Number of successful extensions: 166
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5
Number of HSP's successfully gapped in prelim test: 5
Number of HSP's that attempted gapping in prelim test: 159
Number of HSP's gapped (non-prelim): 10
length of query: 365
length of database: 2,362,478
effective HSP length: 74
effective length of query: 291
effective length of database: 1,992,182
effective search space: 579724962
effective search space used: 579724962
T: 11
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.6 bits)
S2: 54 (25.8 bits)
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